Methods for diagnosing and treating heart disease

ABSTRACT

The invention provides methods of diagnosing heart disease, such as heart failure, screening methods for identifying compounds that can be used to treat or to prevent heart disease, and methods of using these compounds to treat or to prevent heart disease. The invention also provides animal model systems for carrying out the screening methods.

CROSS REFERENCE TO RELATED APPLICATION

[0001] This application is a continuation of and claims priority from U.S. Ser. No. 09/759,508, filed Jan. 12, 2001, which claims benefit of U.S. provisional patent application No. 60/175,787, filed Jan. 12, 2000.

FIELD OF THE INVENTION

[0002] This invention relates to methods for diagnosing and treating heart disease.

BACKGROUND OF THE INVENTION

[0003] Heart disease is a general term used to describe many different heart conditions. For example, coronary artery disease, which is the most common heart disease, is characterized by constriction or narrowing of the arteries supplying the heart with oxygen-rich blood, and can lead to myocardial infarction, which is the death of a portion of the heart muscle. Heart failure is a condition resulting from the inability of the heart to pump an adequate amount of blood through the body. Heart failure is not a sudden, abrupt stop of heart activity, but, rather, typically develops slowly over many years, as the heart gradually loses its ability to pump blood efficiently. Risk factors for heart failure include coronary artery disease, hypertension, valvular heart disease, cardiomyopathy, disease of the heart muscle, obesity, diabetes, and a family history of heart failure.

SUMMARY OF THE INVENTION

[0004] The invention provides diagnostic, drug screening, and therapeutic methods based on the observation that mutation of the titin gene leads to a phenotype in zebrafish that is similar to mammalian heart failure.

[0005] In one aspect, the invention provides a method of determining whether a test subject (e.g., a mammal, such as a human) has, or is at risk of developing, a titin-related disease or condition (e.g., heart failure). This method involves analyzing a nucleic acid molecule of a sample from the test subject to determine whether the test subject has a mutation (for example, a mutation in a cardiac-specific exon, such as the N2B exon; e.g., the pickwick mutation; see below) in a titin gene. The presence of such a mutation is an indication that the test subject has, or is at risk of developing, a titin-related disease. This method can further involve using nucleic acid molecule primers specific for the titin gene for nucleic acid molecule amplification of the titin gene by the polymerase chain reaction, or sequencing titin nucleic acid molecules from the test subject.

[0006] In another aspect, the invention provides a screening method for identifying a compound that can be used to treat or to prevent heart failure. This method involves contacting an organism (e.g., a zebrafish) having a titin mutation (for example, a mutation in a cardiac-specific exon, such as the N2B exon; e.g., the pickwick mutation) and a phenotype characteristic of heart failure with the compound, and determining the effect of the compound on the phenotype. Detection of an improvement in the phenotype indicates the identification of a compound that can be used to treat or to prevent heart failure.

[0007] In another aspect, the invention provides a method of treating or preventing heart disease, such as heart failure, in a patient. This method involves administering to the patient a compound identified using the screening method described above. A patient treated using this method can have a mutation in the titin gene.

[0008] In a further aspect, the invention provides a non-human animal (e.g., a zebrafish or a mouse) that has a mutation in a titin gene. The mutation can be, for example, in a cardiac-specific exon of the titin gene, such as the N2B exon, and can result in production of a truncated titin product, for example, by the introduction of a stop codon.

[0009] By “polypeptide” or “polypeptide fragment” is meant a chain of two or more amino acids, regardless of any post-translational modification (e.g., glycosylation or phosphorylation), constituting all or part of a naturally or non-naturally occurring polypeptide. By “post-translational modification” is meant any change to a polypeptide or polypeptide fragment during or after synthesis. Post-translational modifications can be produced naturally (such as during synthesis within a cell) or generated artificially (such as by recombinant or chemical means). A “protein” can be made up of one or more polypeptides.

[0010] By “titin,” “titin protein,” or “titin polypeptide” is meant a polypeptide that has at least 45%, preferably at least 60%, more preferably at least 75%, and most preferably at least 90% amino acid sequence identity to the sequence of the human (see below) or the zebrafish titin polypeptides. Polypeptide products from splice variants of titin gene sequences and titin genes containing mutations are also included in this definition. A titin polypeptide as defined herein plays a role in heart development, modeling, structure, and contractility. It can be used as a marker of heart disease, such as heart failure.

[0011] By a “titin nucleic acid molecule” is meant a nucleic acid molecule, such as a genomic DNA, cDNA, or RNA (e.g., mRNA) molecule, that encodes titin, a titin protein, a titin polypeptide, or a portion thereof, as defined above.

[0012] The term “identity” is used herein to describe the relationship of the sequence of a particular nucleic acid molecule or polypeptide to the sequence of a reference molecule of the same type. For example, if a polypeptide or nucleic acid molecule has the same amino acid or nucleotide residue at a given position, compared to a reference molecule to which it is aligned, there is said to be “identity” at that position. The level of sequence identity of a nucleic acid molecule or a polypeptide to a reference molecule is typically measured using sequence analysis software with the default parameters specified therein, such as the introduction of gaps to achieve an optimal alignment (e.g., Sequence Analysis Software Package of the Genetics Computer Group, University of Wisconsin Biotechnology Center, 1710 University Avenue, Madison, Wis. 53705, BLAST, or PILEUP/PRETTYBOX programs). These software programs match identical or similar sequences by assigning degrees of identity to various substitutions, deletions, or other modifications. Conservative substitutions typically include substitutions within the following groups: glycine, alanine, valine, isoleucine, and leucine; aspartic acid, glutamic acid, asparagine, and giutamine; serine and threonine; lysine and arginine; and phenylalanine and tyrosine.

[0013] A nucleic acid molecule or polypeptide is said to be “substantially identical” to a reference molecule if it exhibits, over its entire length, at least 51%, preferably at least 55%, 60%, or 65%, and most preferably 75%, 85%, 90%, or 95% identity to the sequence of the reference molecule. For polypeptides, the length of comparison sequences is at least 16 amino acids, preferably at least 20 amino acids, more preferably at least 25 amino acids, and most preferably at least 35 amino acids. For nucleic acid molecules, the length of comparison sequences is at least 50 nucleotides, preferably at least 60 nucleotides, more preferably at least 75 nucleotides, and most preferably at least 110 nucleotides.

[0014] A titin nucleic acid molecule or titin polypeptide is “analyzed” or subject to “analysis” if a test procedure is carried out on it that allows the determination of its biological activity or whether it is wild type or mutated. For example, one can analyze the titin genes of an animal (e.g., a human or a zebrafish) by amplifying genomic DNA of the animal using the polymerase chain reaction, and then determining whether the amplified DNA contains a mutation, for example, the pickwick mutation, by, e.g., nucleotide sequence or restriction fragment analysis.

[0015] By “probe” or “primer” is meant a single-stranded DNA or RNA molecule of defined sequence that can base pair to a second DNA or RNA molecule that contains a complementary sequence (“target”). The stability of the resulting hybrid depends upon the extent of the base pairing that occurs. This stability is affected by parameters such as the degree of complementarity between the probe and target molecule, and the degree of stringency of the hybridization conditions. The degree of hybridization stringency is affected by parameters such as the temperature, salt concentration, and concentration of organic molecules, such as formamide, and is determined by methods that are well known to those skilled in the art. Probes or primers specific for titin nucleic acid molecules, preferably, have greater than 45% sequence identity, more preferably at least 55-75% sequence identity, still more preferably at least 75-85% sequence identity, yet more preferably at least 85-99% sequence identity, and most preferably 100% sequence identity to the sequences of human (see below) or zebrafish titin.

[0016] Probes can be detectably-labeled, either radioactively or non-radioactively, by methods that are well-known to those skilled in the art. Probes can be used for methods involving nucleic acid hybridization, such as nucleic acid sequencing, nucleic acid amplification by the polymerase chain reaction, single stranded confornational polymorphism (SSCP) analysis, restriction fragment polymorphism (RFLP) analysis, Southern hybridization, northern hybridization, in situ hybridization, electrophoretic mobility shift assay (EMSA), and other methods that are well known to those skilled in the art.

[0017] A molecule, e.g., an oligonucleotide probe or primer, a gene or fragment thereof, a cDNA molecule, a polypeptide, or an antibody, can be said to be “detectably-labeled” if it is marked in such a way that its presence can be directly identified in a sample. Methods for detectably-labeling molecules are well known in the art and include, without limitation, radioactive labeling (e.g., with an isotope, such as ³²P or ³⁵S) and nonradioactive labeling (e.g., with a fluorescent label, such as fluorescein).

[0018] By a “substantially pure polypeptide” is meant a polypeptide (or a fragment thereof) that has been separated from proteins and organic molecules that naturally accompany it. Typically, a polypeptide is substantially pure when it is at least 60%, by weight, free from the proteins and naturally-occurring organic molecules with which it is naturally associated. Preferably, the polypeptide is a titin polypeptide that is at least 75%, more preferably at least 90%, and most preferably at least 99%, by weight, pure. A substantially pure titin polypeptide can be obtained, for example, by extraction from a natural source (e.g., isolated heart tissue), by expression of a recombinant nucleic acid molecule encoding a titin polypeptide, or by chemical synthesis. Purity can be measured by any appropriate method, e.g., by column chromatography, polyacrylamide gel electrophoresis, or HPLC analysis.

[0019] A polypeptide is substantially free of naturally associated components when it is separated from those proteins and organic molecules that accompany it in its natural state. Thus, a protein that is chemically synthesized or produced in a cellular system different from the cell in which it is naturally produced is substantially free from its naturally associated components. Accordingly, substantially pure polypeptides not only include those derived from eukaryotic organisms, but also those synthesized in E. coli or other prokaryotes.

[0020] An antibody is said to “specifically bind” to a polypeptide if it recognizes and binds to the polypeptide (e.g., a titin polypeptidc), but does not substantially recognize and bind to other molecules (e.g., non-titin related polypeptides) in a sample, e.g., a biological sample that naturally includes the polypeptide.

[0021] By “high stringency conditions” is meant conditions that allow hybridization comparable with the hybridization that occurs using a DNA probe of at least 500 nucleotides in length, in a buffer containing 0.5 M NaHPO₄, pH 7.2, 7% SDS, 1 mM EDTA, and 1% BSA (fraction V), at a temperature of 65° C., or a buffer containing 48% formamide, 4.8×SSC, 0.2 M Tris-Cl, pH 7.6, 1× Denhardt's solution, 10% dextran sulfate, and 0.1% SDS, at a temperature of 42° C. (These are typical conditions for high stringency northern or Southern hybridizations.) High stringency hybridization is also relied upon for the success of numerous techniques routinely performed by molecular biologists, such as high stringency PCR, DNA sequencing, single strand conformational polymorphism analysis, and in situ hybridization. In contrast to northern and Southern hybridizations, these techniques are usually performed with relatively short probes (e.g., usually 16 nucleotides or longer for PCR or sequencing and 40 nucleotides or longer for in situ hybridization). The high stringency conditions used in these techniques are well known to those skilled in the art of molecular biology, and examples of them can be found, for example, in Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, New York, N.Y., 1998, which is hereby incorporated by reference.

[0022] By “sample” is meant a tissue biopsy, amniotic fluid, cell, blood, serum, urine, stool, or other specimen obtained from a patient or test subject. The sample can be analyzed to detect a mutation in a titin gene, or expression levels of a titin gene, by methods that are known in the art. For example, methods such as sequencing, single-strand conformational polymorphism (SSCP) analysis, or restriction fragment length polymorphism (RFLP) analysis of PCR products derived from a patient sample can be used to detect a mutation in a titin gene; ELISA can be used to measure levels of titin polypeptide; and PCR can be used to measure the level of a titin nucleic acid molecule.

[0023] By “titin-related disease” or “titin-related condition” is meant a disease or condition that results from inappropriately high or low expression of a titin gene, or a mutation in a titin gene that alters the biological activity of a titin nucleic acid molecule or polypeptide. Titin-related diseases and conditions can arise in any tissue in which titin is expressed during prenatal or post-natal life. Titin-related diseases and conditions can include heart diseases, such as heart failure. Specific examples of different types of heart failure are provided below.

[0024] The invention provides several advantages. For example, using the diagnostic methods of the invention, it is possible to detect an increased likelihood of heart disease, such as heart failure, in a patient, so that appropriate intervention can be instituted before any symptoms occur. This may be useful, for example, with patients in high-risk groups for heart failure (see above). Also, the diagnostic methods of the invention facilitate determination of the etiology of an existing heart condition, such as heart failure, in a patient, so that an appropriate approach to treatment can be selected. In addition, the screening methods of the invention can be used to identify compounds that can be used to treat or to prevent heart conditions, such as heart failure.

[0025] Other features and advantages of the invention will be apparent from the following detailed description and the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

[0026]FIG. 1 is a schematic representation of the domain structure of the human titin filament.

[0027] The nucleotide and amino acid sequences of human titin are provided in SEQ ID NOs:1 and 2, respectively. The modular architecture of cardiac titin as predicted by its full-length cDNA is shown.

[0028] A total of 244 copies of 100 residue repeats, as indicated by vertical rectangles, are contained in the molecule. One hundred and twelve of these belong to the Ig domain, and 132 belong to the FN3 superfamily. The titin kinase domain, as well as the PEVK element N2-B 163-residue variant are also shown. Within the A-band , the D-zone contains six tandem repeats of the seven domains shown (A1 through A42), and the C-zone contains 11 tandem repeats of the 11 domains shown (A43 through A163). The positions of the tandemly repeated RMSP and VKSP motifs in the Z-disc and M-line region are also shown

DETAILED DESCRIPTION

[0029] The invention provides methods of diagnosing heart disease, screening methods for identifying compounds that can be used to treat or to prevent heart disease, and methods of treating or preventing heart disease using these compounds. The invention also provides animal model systems that can be used in the screening methods of the invention.

[0030] In particular, we have discovered that a mutation (the pickwick mutation) in the titin gene is associated with heart disease, such as heart failure. Titin, which is also known as “connectin,” is the largest known single-chain protein, having a molecular weight of about 3,000 kDa. Titin is a structural protein, and plays a central role in the assembly and elasticity of vertebrate skeletal and cardiac muscle. Thus, the diagnostic methods of the invention involve detection of mutations in the titin gene, while the compound identification methods of the invention involve screening for compounds that affect the phenotype of titin mutants or other models of heart disease, such as heart failure. Compounds identified in this manner can be used in methods to treat or to prevent heart disease (e.g., heart failure). The diagnostic, screening, and therapeutic methods of the invention, as well as the animal model systems of the invention, are described further, as follows.

[0031] Diagnostic Methods

[0032] Titin nucleic acid molecules, polypeptides, and antibodies can be used in methods to diagnose or to monitor diseases and conditions involving mutations in, or inappropriate expression of, titin genes. As discussed further below, the pickwick mutation in zebrafish, which is present in the titin gene, is characterized by a phenotype that is similar to that of heart failure in humans. Thus, detection of abnormalities in titin genes or their expression can be used in methods to diagnose, or to monitor treatment or development of, human heart disease, such as heart failure. For use as references, the human cardiac titin cDNA sequence can be found at: http://www.embl-heidelberg.de/ExternalInfo/Titin/cardiacpep.html (SEQ ID NO:1), while the corresponding protein sequence can be found at:

[0033] http://www.embl-heidelberg.de/ExternalInfo/Titin/cardiacpep.html (SEQ ID NO:2).

[0034] As noted above, the diagnostic methods of the invention can be used, for example with patients that have heart failure, in an effort to determine its etiology and, thus, to facilitate selection of an appropriate course of treatment. The diagnostic methods can also be used with patients that have not yet developed heart failure, but who are at risk of developing such a disease, or with patients that are at an early stage of developing such a disease. Also, the diagnostic methods of the invention can be used in prenatal genetic screening, for example, to identify parents who may be carriers of a recessive titin mutation.

[0035] Examples of heart failure that can be diagnosed (and treated) using the methods of the invention include congestive heart failure, which is characterized by fluid in the lungs or body, resulting from failure of the heart in acting as a pump; right sided heart failure (right ventricular), which is characterized by failure of the pumping action of the right ventricle, resulting in swelling of the body, especially the legs and abdomen; left sided heart failure (left ventricular), which is caused by failure of the pumping action of the left side of the heart, resulting in congestion of the lungs; forward heart failure, which is characterized by the inability of the heart to pump blood forward at a sufficient rate to meet the oxygen needs of the body at rest or during exercise; backward heart failure, which is characterized by the ability of the heart to meet the needs of the body only if heart filling pressures are abnormally high; low-output, which is characterized by failure to maintain blood output; and high-output, which is characterized by heart failure symptoms, even when cardiac output is high.

[0036] Titin may also play a role in cardiovascular diseases other than heart failure, such as coronary artery disease or conditions associated with valve formation defects, and, thus, detection of abnormalities in titin genes or their expression can be used in methods to diagnose and monitor these conditions as well. The methods of the invention can be used to diagnose (or to treat) the disorders described herein in any mammal, for example, humans, domestic pets, or livestock.

[0037] Titin abnormalities that can be detected using the diagnostic methods of the invention include those characterized by, for example, (i) abnormal titin polypeptides, (ii) titin genes containing mutations that result in the production of such polypeptides, and (iii) titin mutations that result in production of abnormal amounts of titin. Detection of such abnormalities, thus, can be used in methods to diagnose human heart disease, such as heart failure. Exemplary of the titin mutations that can be detected using the methods of the invention is the pickwick mutation (see below).

[0038] Detection of titin mutations can be carried out using any diagnostic technique. For example, a biological sample obtained from a patient can be analyzed for one or more mutations in titin nucleic acid molecules (e.g., the pickwick mutation) using a mismatch detection approach. Generally, this approach involves polymerase chain reaction (PCR) amplification of nucleic acid molecules from a patient sample, followed by identification of a mutation (i.e., a mismatch) by detection of altered hybridization, aberrant electrophoretic gel migration, binding, or cleavage mediated by mismatch binding proteins, or by direct nucleic acid molecule sequencing. Any of these techniques can be used to facilitate detection of mutant titin genes, and each is well known in the art. Examples of these techniques are described by Orita et al. (Proc. Natl. Acad. Sci. U.S.A. 86:2766-2770, 1989) and Sheffield et al. (Proc. Natl. Acad. Sci. U.S.A. 86:232-236, 1989).

[0039] Mutation detection assays also provide an opportunity to diagnose a titin-mediated predisposition to heart disease before the onset of symptoms. For example, a patient heterozygous for a titin mutation that suppresses normal titin biological activity or expression may show no clinical symptoms of a titin-related disease, and yet possess a higher than normal probability of developing heart disease, such as heart failure. Given such a diagnosis, a patient can take precautions to minimize exposure to adverse environmental factors, and can carefully monitor their medical condition, for example, through frequent physical examinations. As mentioned above, this type of diagnostic approach can also be used to detect titin mutations in prenatal screens.

[0040] The titin diagnostic assays described above can be carried out using any biological sample (for example, a muscle tissue sample) in which titin is normally expressed. Because of the limited number of tissues in which titin is expressed, as well as the relative difficulties involved in obtaining samples of these tissues, it may be preferable to detect mutant titin genes in another, more easily obtained sample type, such as blood or amniotic fluid samples using, for example, mismatch detection techniques. Preferably, the DNA in such a sample is subjected to PCR amplification prior to analysis.

[0041] Levels of titin expression in a patient sample can be determined by using any of a number of standard techniques that are well known in the art. For example, titin expression in a biological sample (e.g., a blood or tissue sample, or amniotic fluid) from a patient can be monitored by standard northern blot analysis or by quantitative PCR (see, e.g., Ausubel et al., supra; PCR Technology: Principles and Applications for DNA Amplification, H. A. Ehrlich, Ed., Stockton Press, NY; Yap et al., Nucl. Acids. Res. 19:4294, 1991).

[0042] In yet another diagnostic approach of the invention, an immunoassay is used to detect or to monitor titin protein levels in a biological sample. Titin-specific polyclonal or monoclonal antibodies can be used in any standard immunoassay format (e.g., ELISA, Western blot, or RIA; see, e.g., Ausubel et al., supra) to measure titin polypeptide levels. These levels are compared to wild-type titin levels. For example, a decrease in titin production may be indicative of a condition or a predisposition to a condition involving insufficient titin biological activity.

[0043] Immunohistochemical techniques can also be utilized for titin detection. For example, a tissue sample can be obtained from a patient, sectioned, and stained for the presence of titin using an anti-titin antibody and any standard detection system (e.g., one that includes a secondary antibody conjugated to horseradish peroxidase). General guidance regarding such techniques can be found in, e.g., Bancroft et al., Theory and Practice of Histological Techniques, Churchill Livingstone, 1982, and in Ausubel et al., supra.

[0044] Identification of Molecules That Can Be Used to Treat or to Prevent Heart Failure

[0045] Identification of a mutation in titin as resulting in a phenotype that is related to heart failure facilitates the identification of molecules (e.g., small organic molecules, peptides, or nucleic acid molecules) that can be used to treat or to prevent heart failure. The effects of candidate compounds on heart failure can be investigated using, for example, the zebrafish system. The zebrafish, Danio rerio, is a convenient organism to use in genetic analysis of vascular development. In addition to its short generation time and fecundity, it has an accessible and transparent embryo, allowing direct observation of blood vessel finction from the earliest stages. As discussed further below, zcbrafish and other animals having mutations in the titin gene, which can be used in these methods, are also included in the invention.

[0046] In one example of the screening methods of the invention, a zebrafish having a mutation in the titin gene (e.g., a zebrafish having the pickwick mutation; see below) is contacted with a candidate compound, and the effect of the compound on the development of a heart abnormality that is characteristic of heart failure, or on the status of such an existing heart abnormality, is monitored, relative to an untreated, identically mutant control. As discussed further below, zebrafish having the pickwick mutation are characterized by, for example, reduction of peak systolic pressures, stretched and thin myocardium, excess cardiac jelly, absent A-V cushions, and an obstructed ventricular outflow tract. Thus, these characteristics (in addition to other characteristics of heart failure) can be monitored using the screening methods of the invention.

[0047] After a compound has been shown to have a desired effect in the zebrafish system, it can be tested in other models of heart disease, for example, in mice or other animals having a mutation in the titin gene. Alternatively, testing in such animal model systems can be carried out in the absence of zebrafish testing.

[0048] Candidate compounds can be purified (or substantially purified) molecules or can be one component of a mixture of compounds (e.g., an extract or supernatant obtained from cells; Ausubel et al., supra). In a mixed compound assay, the effect on a phenotype of heart failure is tested against progressively smaller subsets of the candidate compound pool (e.g., produced by standard purification techniques, e.g., HPLC or FPLC) until a single compound or minimal compound mixture is demonstrated to have the desired effect.

[0049] Test compounds that can be screened in the methods described above can be chemicals that are naturally occurring or artificially derived. Such compounds can include, for example, polypeptides, synthesized organic molecules, naturally occurring organic molecules, nucleic acid molecules, and components thereof. Candidate compound can be found, for example, in a cell extract, mammalian serum, or growth medium in which mammalian cells have been cultured.

[0050] In general, novel drugs for prevention or treatment of mutant titin-related heart diseases can be identified from large libraries of both natural products, synthetic (or semi-synthetic) extracts, and chemical libraries using methods that are well known in the art. Those skilled in the field of drug discovery and development will understand that the precise source of test extracts or compounds is not critical to the screening methods of the invention. Accordingly, virtually any number of chemical extracts or compounds can be screened using these methods. Examples of such extracts or compounds include, but are not limited to, plant-, fungal-, prokaryotic-, or animal-based extracts, fermentation broths, and synthetic compounds, as well as modifications of existing compounds.

[0051] Numerous methods are also available for generating random or directed synthesis (e.g., semi-synthesis or total synthesis) of any number of chemical compounds, including, but not limited to, saccharide-, lipid-, peptide-, and nucleic acid molecule-based compounds. Synthetic compound libraries are commercially available from Brandon Associates (Merrimack, N.H.) and Aldrich Chemical (Milwaukee, Wis.). Alternatively, libraries of natural compounds in the form of bacterial, fungal, plant, and animal extracts are commercially available from a number of sources, including Biotics (Sussex, UK), Xenova (Slough, UK), Harbor Branch Oceanographic Institute (Ft. Pierce, Fla.), and PharmaMar, U.S.A. (Cambridge, Mass.). In addition, natural and synthetically produced libraries can be produced, if desired, according to methods known in the art, e.g., by standard extraction and fractionation. Furthermore, if desired, any library or compound can be readily modified using standard chemical, physical, or biochemical methods.

[0052] In addition, those skilled in the art of drug discovery and development readily understand that methods for dereplication (e.g., taxonomic dereplication, biological dereplication, and chemical dereplication, or any combination thereof) or the elimination of replicates or repeats of materials already known for their therapeutic activities for heart failure can be employed whenever possible.

[0053] When a crude extract is found to have an effect on the development or persistence of heart failure, further fractionation of the positive lead extract can be carried out to isolate chemical constituents responsible for the observed effect. Thus, the goal of the extraction, fractionation, and purification process is the careful characterization and identification of a chemical entity within the crude extract having a desired activity. The same assays described herein for the detection of activities in mixtures of compounds can be used to purify the active component and to test derivatives of these compounds. Methods of fractionation and purification of such heterogeneous extracts are well known in the art. If desired, compounds shown to be useful agents for treatment can be chemically modified according to methods known in the art.

[0054] Treatment or Prevention of Heart Failure

[0055] Compounds identified using the screening methods described above can be used to treat patients that have or are at risk of developing heart disease, such as heart failure. Such treatment may be required only for a short period of time, or may, in some form, be required throughout a patient's lifetime. Any continued need for treatment, however, can be determined using, for example, the diagnostic methods described above. In considering various therapies, it is understood that such therapies are, preferably, targeted to the affected or potentially affected organ, that is, the heart.

[0056] Treatment or prevention of diseases resulting from a mutated titin gene can be accomplished, for example, by modulating the function of a mutant titin protein, delivering normal titin protein to the appropriate cells, altering the levels of normal or mutant titin protein, replacing a mutant titin gene with a normal titin gene or, administering a normal titin gene. It is also possible to correct a titin defect by modifying the physiological pathway (e.g., a signal transduction pathway) in which the titin protein participates.

[0057] In a patient diagnosed as heterozygous for a titin mutation, or as susceptible to titin mutations or aberrant titin expression (even if those mutations or expression patterns do not yet result in alterations in titin expression or biological activity), any of the above-described therapies can be administered before the occurrence of the disease phenotype. In particular, compounds shown to modulate titin expression or to have an effect on the phenotype of titin mutants can be administered to patients diagnosed with potential or actual heart disease by any standard dosage and route of administration.

[0058] Any appropriate route of administration can be employed to administer a compound found to be effective in treating or preventing heart failure, according to the invention. For example, administration can be parenteral, intravenous, intra-arterial, subcutaneous, intramuscular, intraventricular, intracapsular, intraspinal, intracistemal, intraperitoneal, intranasal, aerosol, or oral. However, as noted above, preferably, the adminstration is local to the afflicted tissue, that is, the heart. Therapeutic formulations can be in the form of liquid solutions or suspensions; for oral administration, formulations can be in the form of tablets or capsules; and for intranasal formulations, in the form of powders, nasal drops, or aerosols.

[0059] A therapeutic compound of the invention can be administered within a pharmaceutically acceptable diluent, carrier, or excipient, in unit dosage form. Administration can begin before or after the patient is symptomatic. Methods that are well known in the art for making formulations are found, for example, in Remington's Pharmaceutical Sciences, (18 ^(th) edition), ed. A. Gennaro, 1990, Mack Publishing Company, Easton, Pa. Formulations for parenteral administration can, for example, contain excipients; sterile water; or saline; polyalkylene glycols, such as polyethylene glycol; oils of vegetable origin; or hydrogenated napthalenes. Biocompatible, biodegradable lactide polymer, lactide/glycolide copolymer, or polyoxyethylene-polyoxypropylene copolymers can be used to control the release of the compounds. Other potentially useful parenteral delivery systems for compounds identified using the methods of the invention include ethylene-vinyl acetate copolymer particles, osmotic pumps, implantable infusion systems, and liposomes. Formulations for inhalation can contain excipients, for example, lactose, or can be aqueous solutions containing, for example, polyoxyethylene-9-lauryl ether, glycocholate, and deoxycholate, or can be oily solutions for administration in the form of nasal drops, or as a gel.

[0060] Titin nucleic acid molecules and polypeptides can also be used in tissue engineering, for example, in the manufacture of artificial or partially artificial hearts. As mentioned above, titin plays a role in cardiovascular elasticity, integrity, and contractility. Thus, a titin nucleic acid molecule or polypeptide, as described above, can be used to impart such characteristics on an artificial or partially artificial heart.

[0061] Animal Model Systems

[0062] The invention also provides animal model systems for use in carrying out the screening methods described above. Examples of these model systems include zebrafish and other animals, such as mice, that have mutations in a titin gene. For example, a zebrafish model that can be used in the invention can include a mutation that results in a lack of titin production or production of a truncated (e.g., by introduction of a stop codon) or otherwise altered titin gene product. The mutation can, for example, result in the presence of a stop codon in a cardiac-specific exon, such as the N2B exon (e.g., in the IS3 region; see below). The mutation can be in a region encoding the I band, resulting in the production of a protein in which the I band is truncated and the A band and M line are absent, or can be in a region encoding another portion of the molecule, such as the A band or M line region. As a specific example, a zebrafish having the pickwick mutation can be used.

[0063] Experimental Results

[0064] During a large-scale mutagenesis screening of the zebrafish genome, a group of mutations were identified that affect cardiac contractility. One of these mutations, called pickwick (pik), dramatically reduces the function of both chambers, causing a recessive, lethal form of heart failure in the zebrafish embryo.

[0065] Direct in vivo recording of ventricular pressures by the null balance feedback system shows that pickwick causes a 5.8 fold reduction of peak systolic pressures, compared to age-matched controls (0.084±0.008 vs. 0.49±0.06 mm Hg). Morphological analysis of pickwick revealed a stretched and thin myocardium, excess cardiac jelly, absent A-V cushions, and an obstructed ventricular outflow tract. Extensive ultrastructural defects were found by transmission electron microscopy, affecting the assembly of Z-discs and the organization of myofilaments. Reciprocal blastomere transplants identified pickwick as a cell autonomously acting mutation of the myoblast lineage.

[0066] A positional cloning approach was adopted for gene identification. The pickwick mutation has been assigned to a small chromosomal interval, which is covered by BAC clones. The titin gene spans this interval, and thus the pickwick phenotype is due to a mutation in the titin gene. In particular, the pickwick gene was mapped to linkage group (LG) 9 by bulk segregant analysis using the pikm242 allele, which is one of five cardiac specific alleles (pikm242, pikm171,pikm740,pikm186,pikmnm2). A panel of Z-markers in the linkage group was tested for simple sequence length polymorphisms (SSLPs) using 931 homozygous mutant embryos. Markers Z8363 and Z26463 were shown to flank the pickwick locus, defining a 1.2 cM interval containing the gene. It is estimated that 1 cM corresponds to 500-600 kb DNA in the zebrafish genome (Postlethwait et al., Science 264:699-703, 1994). We thus initiated chromosomal walking from the Z8363 marker, which is 0.7 cM from the mutation (12 recombinants out of 1750 meioses).

[0067] A positive YAC clone (YAC5) was identified that had a T7 end that is highly homologous to human titin coding sequences. As the titin genomic region was estimated to be over 300 kb in humans, we decided to identify BAC clones based on sequence information of the zebrafish titin EST clones. A physical contig was constructed based on these sequences, which covers the whole titin genomic area. Single stranded conformational polymorphisms (SSCPs) were developed from the ends and internal sequences of these clones were used for fine recombinational mapping. One SSCP marker inside the titin genomic area (B9F2) picked up one recombinant from the Z26463 side. The other four SSCP markers inside the titin genomic area (B4SP1, B2T7, B7SP, and B6SP) picked up zero recombinants out of 1750 meioses. These genetic data indicated that the pickwick locus is very close to or within the titin genomic region.

[0068] As the titin cDNA alone is around 82 kb, rescuing the phenotype by RNA injection could prove to be quite difficult. However, we found evidence that the pickwick locus is within the titin gene by identification of point mutations in one of the pickwick alleles. As most of the alleles ofpickwick have a cardiac-specific phenotype, we presumed that the point mutation is located in a cardiac-specific exon. We focused on the N2B domain in the I-band of titin, as all of the cardiac isoforms are N2B domain based. The zebrafish N2B domain was cloned by RT-PCR. It is a 4.3 kb cDNA encoding infrastructures similar to those in humans and mice, and contains 4 IG repeats and three unique sequences, including longer IS3 and shorter IS1.

[0069] N2B domains from pickwick mutant embryos were then cloned. RNA mismatch analysis was performed to identify the location of the point mutation. One mismatch between the PCR products from pikm171 and pikm242 was identified. Sequencing of the PCR product resulted in the identification of a T→G transition in the pikm171 allele. This mutation resulted in a change of leucine in the IS3 fragment of N2B domain (N2B-IS3) into a stop codon. The mutation was confirmed in all of the seven homozygous pikm171 mutant embryos, but none of the four pikm242 homozygous embryos. A truncated version of titin is predicted to be in the pikm171 mutant, only as a cardiac specific isoform. It should contain the Z-disc and part of the I-band and be sized around 4,000 amino acids, based on comparison to the homologous human titin sequence, which has a full length of 27,000 amino acids. The identification of a non-sense mutation in the cardiac specific N2B domain in pikm171 allele confirmed the hypothesis that titin is the pickwick gene.

[0070] Titin was expressed in the zebrafish embryo during the period when the pickwick phenotype was first detected. Whole mount in situ hybridization analysis indicated that titin was expressed strongly in both the heart and the somites at 24 hpf. Titin mRNA expression in the heart is normal in pikm171. We confirmed the notion that N2B is a cardiac-specific exon in zebrafish by labeling a probe in the IS3 domain for the whole mount in situ hybridization.

[0071] The identification of a point mutation in the N2B domain thus establishes pickwick as the first in vivo vertebrate system to study the functions of titin in the heart. If titin functions as a spring, as proposed, it is expected that the contraction will be much weaker. This is exactly what we observed in pickwick mutant embryos. If titin functions as a template during the sarcomere assembly, a “silent heart” phenotype would be expected in titin null mutation. According to the current model of the myofibrillogenesis (Dabiri et al., Proc. Natl. Acad. Sci. U.S.A. 94:9493-9498, 1997), the thick element and/or the sarcomere could not assemble into a beating machine. In contrast, the hearts in the homozygous pikm171 mutant embryos and all of the other pickwick alleles still beat, despite being weaker.

[0072] The mutation in pikm171 predicts a truncated protein in which most of the elastic I-band is deleted and the C-terminal A-band and M-line regions eliminated. It thus could be considered as a null mutation in terms of function as a spring and a potential dominant negative mutation in terms of its function as a template for sarcomere assembly (Tumacioglu et al., Mol. Biol. Cell 8:705-717, 1997). The observation of the weak beating in thepickwick mutant embryos suggested the existence of primary contractile machinery without titin. Indeed, thick and thin elements can be detected in the ventricular myocardium cells. They have the capacity to assemble into a functional beating structure in the absence of titin.

[0073] We thus have carried out a detailed physiological and morphological analysis of pickwick, a zebrafish heart function mutation that reduces the contractility of both chambers. Several pieces of evidence pointed out that titin is the pickwick gene. Genetic analysis linked the pickwick locus closely to the titin genomic area. The identification of the pikmVO62H, a pickwick allele that has an additional somite phenotype, can be explained by the titin hypothesis. The point mutation is expected to be in the common exons that are shared between the cardiac and somatic isoforms of titin. Evidence confirming this hypothesis came from the identification of a point mutation in the cardiac specific N2B domain of titin in one of the pickwick alleles, pikm171. We went on to show that sarcomere structure is disrupted in the myocardium cells of pikm171, but not the somatic muscle cells. The expression pattern of titin is consistent with this phenotype. Strong expression in both cardiac and skeletal muscles was detected at the onset of the pickwick phenotype.

[0074] Thus, our observation in zebrafish is in consistent with the notion that titin functions as a spring during the muscle contraction. As titin is a sarcomere structure protein, it is conceivable that myocardium is affected cell-autonomously in pickwick mutant embryos. The thin and stretched morphology could be due to the mechanic tension generated from the failure to form higher-order sarcomere structure and the loss of spring. The mechanic tension may also be the reason for the separation between the myocardium cells and endocardium cells, generating the excess cardiac jelly. However, there is a possibility that the differentiation program of the myocardium was affected in the titin mutation. The valve formation phenotype in pickwick mutant embryos could be a secondary defect. It has been suggested that the process of endothelial invasion during valve formation is under control of a localized myocardial signal. (For a review, see Fishman et al., Development 124:2099-2117, 1997.) The physical distance between myocardium and endocardium and/or the stalked differential program in the myocardium could be the reason that prevents a normal cushion formation.

[0075] Material and Methods

[0076] Zebrafish Strains and Maintenance

[0077] Zebrafish were maintained and staged as described. pikm242, pikm171, pikm740, pikm186were generated in a screen on the AB background (Stainier et. al., Development 123:285-292, 1996). pikm VO62H and pikmnml2 were generated in a screen on the TL background. Mapping strains were constructed by crossing pikm242 into india strain. pikm171 embryos used in expression analysis and EM were obtained from pair wise matings of pikm171/TL heterozygotes.

[0078] In situ Hybridization

[0079] Whole mount in situ hybridization was performed as described (Thisse et al., Development 119:1203-1215, 1993). T5 probe was generated by digestion of the EST clone AI629069 (Research Genetics). The N2B and N2A probe were generated through PCR with a tagged T7 promoter. The primer pairs are: P238F: 5′-AGGGACACTCAGAGACCATAG; (SEQ ID NO:3) and P3785RT: 5′-TAATACGACTCACTATAGGGGTCTGAGGATACTCGCCTTC. (SEQ ID NO:4)

[0080] Mapping of Pickwick

[0081] Linkage was established using DNA from 16 homozygous mutations and 16 heterozygous or wild type pick pikm242/indian embryos in bulk segregation analysis (Michelmore et al., Proc. Natl. Acad. Sci. U.S.A. 88:9828-9832, 1991). Z-markers (simple sequence length polymorphisms, SSLP) were developed in this lab (Knapik et al., Nat. Genet. 18:338-343, 1998; Shimoda et al., Genomics 58:219-232, 1999). Genotyping products were resolved in 6% PAGE gels.

[0082] Chromosomal Walking

[0083] YAC and BAC clones were screened by PCR as pools of clones (Research Genetics) according to the manufacturer's instruction. YAC ends were cloned by plasmid rescue (Zhong et al., Genomics 48:136-138, 1998). Chimeric ends were determined by the RH panel (Geisler et al., Nat. Genet. 23:86-89, 1999). BAC DNA was extracted using the QIAgene kit and the end sequences were determined by direct sequencing. BLAST-X was performed to search for homologous sequence in Genebank. EST clones were generated by Washington University zebrafish EST project and obtained from Research Genetics. Oligonucleotides derived from the end sequences of YAC and BAC clones were used in standard PCR reactions to determine clone overlap. Primer pair B9F2 was derived from a fragment that was generated by digesting BAC5 with BamHI and then subcloning into the pUC19 vector. This clone can be hit by a primer pair derived form the T7 end of BAC7. Single-strand conformation polymorphisms (SSCP) were tested on 6% MDE acrylamide (FMC Bioproducts) gels at 40° C.

[0084] Cloning of the Zebrafish N2B Domain

[0085] Long RT-PCR was performed to amplify the N2B domain from adult zebrafish heart mRNA extracts. mRNA was extracted from pools of ten zebrafish adult hearts using TRIzole reagent (GibcolBRL), as described. The cDNA was synthesized using SuperScripTMII RNase H-Reverse Transcriptase (GibcoBRL) and then treated with RNase H. The primer is derived from EST3:I19R1: 5′-TTTGAACCACTTGAAGGTCACACCAGG. (SEQ ID NO:5)

[0086] Long-PCR was performed using Expand 20 kbPlus PCR System (Roch) as described. The primer pairs are: I14F1: 5′-GCTAAGAATGACTATGGAGTTGCCACAAGC (SEQ ID NO:6) I19R2: 5′-TGAACCACTTGAAGGTCACACCAGGAG (SEQ ID NO:7)

[0087] The 4.6 kb product was subcloned using TOPO TA Cloning Kit (Invitrogen) as described. The sequence was determined by primer walking. Forty eight reads were aligned by Phred/Phrad software to get a large contig that contains only one reading frame with around three fold coverage.

[0088] To amplify the N2B region from the homozygous mutant zebrafish embryos, three overlapping primer pairs were designed according to the adult N2B sequence. The contamination from the skeletal muscle specific titin isoform was thus eliminated. mRNA was extracted from pools of ten zebrafish day 2 embryos using TRIzole reagent (GibcoBRL) as described. Sequencing results indicated that the embryonic heart titin contain one less IG domain

[0089] Identification of the Point Mutation

[0090] N2B domains from the homozygous mutant embryos were further amplified by primer pairs that generate six overlapping PCR products sized between 0.6 kb and about 1 kb. RNA mismatch analysis was performed using MutationScreenerTM (Ambion) according to the manufacture's protocol. A mismatch was identified between pikm171 and pikm242 using the primer pairs: P238FT: 5′-TAATACGACTCACTATAGGGAGGGACACTCAGAGACCATAG (SEQ ID NO:8) P3785RT: 5′-TAATACGACTCACTATAGGGGTCTGAGGATACTCGCCTTC. (SEQ ID NO:9)

[0091] Sequencing results indicate a T→G non-sense mutation in the cDNA from homozygous pikm171 embryos.

[0092] Genomic sequence in this region was amplified using the primer pair: P238F: 5′-AGGGACACTCAGAGACCATAG (SEQ ID NO:10) P341R: 5′-GGCAATGTTACTCTCTGTTGAG (SEQ ID NO:11)

[0093] and sent out directly for sequencing after purification using the Geneclean Spin Kit (BIO101).

[0094] Electron Microscopy

[0095] Forty eight hour embryos were fixed overnight at 4° C. in 1.5% glutaraldehyde, 1% paraformaldehyde, 70 mM NaPO₄ pH 7.2, 3% Sucrose. They were then washed 3 times for 5 minutes each in 0.1M cacodylate buffer, pH 7.4.

OTHER EMBODIMENTS

[0096] Although the present invention has been described with reference to preferred embodiments, one skilled in the art can easily ascertain its essential characteristics and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed in the scope of the present invention.

[0097] All publications and patents mentioned in this specification are hereby incorporated by reference.

1 11 1 81940 DNA Homo sapiens 1 attagaggct caccgattca tgtcggagat ggtcagaaaa accaactctc cataggacgt 60 cgtttcagaa gcaaccttgg gcttagtccc acccttttta ggcactcttg agaaatcaga 120 gtgcctagaa agatgacaac tcaagcaccg acgtttacgc agccgttaca aagcgttgtg 180 gtactggagg gtagtaccgc aacctttgag gctcacatta gtggttttcc agttcctgag 240 gtgagctggt ttagggatgg ccaggtgatt tccacttcca ctctgcccgg cgtgcagatc 300 tcctttagcg atggccgcgc taaactgacg atccccgccg tgactaaagc caacagtgga 360 cgatattccc tgaaagccac caatggatct ggacaagcga ctagtactgc tgagcttctc 420 gtgaaagctg agacagcacc acccaacttc gttcaacgac tgcagagcat gaccgtgaga 480 caaggaagcc aagtgagact ccaagtgaga gtgactggaa tccctacacc tgtggtgaag 540 ttctaccggg atggagccga aatccagagc tcccttgatt tccaaatttc acaagaaggc 600 gacctctaca gcttactgat tgcagaagca taccctgagg actcagggac ctattcagta 660 aatgccacca atagcgttgg aagagctact tcgactgctg aattactggt tcaaggtgaa 720 gaagaagtac ctgctaaaaa gacaaagaca attgtttcga ctgctcagat ctcagaatca 780 agacaaaccc gaattgaaaa gaagattgaa gcccactttg atgccagatc aattgcaaca 840 gttgagatgg tcatagatgg tgccgctggg caacagctgc cacataaaac acctcccagg 900 attcctccga agccaaagtc aagatcccca acaccaccgt ctattgctgc caaagcacag 960 ctggctcggc agcagtcccc atcgcccata agacactccc cttccccggt cagacacgtg 1020 cgggcaccga ccccatctcc ggtcaggtcc gtgtctccag cagcaagaat ctccacatcc 1080 cccatcaggt ctgttaggtc tccattgctc atgcgtaaga ctcaggcatc caccgtggcc 1140 acaggtcctg aagtgcctcc cccttggaag caagagggct acgtggcctc ctcatctgag 1200 gctgagatga gagagacaac gctgacaacc tctactcaga tcaggacaga agagagatgg 1260 gaagggagat acggtgtcca ggagcaagtg accatcagtg gtgctgcggg tgctgccgcc 1320 agtgtgtcgg ccagtgctag ctacgcagca gaggctgttg ccactggtgc taaagaggtg 1380 aaacaagatg ctgacaaaag tgcagctgtt gcgactgttg ttgctgccgt tgatatggcc 1440 agagtgagag aaccagtgat cagcgctgta gagcagactg ctcagaggac aaccacgact 1500 gctgtgcaca tccaacctgc tcaagaacag gtaagaaagg aagcggagaa gactgctgta 1560 actaaggtag tagtggccgc cgataaagcc aaggaacaag aattaaaatc aagaaccaaa 1620 gaaataatta ccacaaagca agagcagatg cacgtaactc atgagcagat aagaaaagaa 1680 actgaaaaaa catttgtacc aaaggtagta atttccgcag ctaaagccaa agaacaagaa 1740 actagaattt ctgaagaaat tactaagaaa cagaaacaag taactcaaga agcaataatg 1800 aaggaaacta ggaaaacagt tgtacctaaa gtcatagttg ccacacccaa agtcaaagaa 1860 caagatttag tatcaagagg tagagaaggc attactacca aaagagaaca agtgcaaata 1920 actcaggaga agatgagaaa ggaagccgag aaaactgcct tgtctacaat agcagttgct 1980 actgctaaag ccaaagaaca agaaacaata ctgagaacta gagaaactat ggctactaga 2040 caagaacaaa tccaagttac ccatggaaag gtggacgttg gaaaaaaggc tgaagctgta 2100 gcaacagttg ttgctgcagt agaccaggcc cgagtcagag agcccagaga gcctgggcat 2160 cttgaagaat cctatgctca gcagaccact ttggagtacg gatataagga acgcatttcc 2220 gccgcaaagg tagctgagcc tccccaacgt ccagcctcag aaccccacgt tgtccctaaa 2280 gcagtcaagc ctagagtaat ccaggctcct tctgagactc atatcaaaac tactgatcaa 2340 aagggaatgc acatatcatc acagatcaag aaaactacag atctaacaac ggaaagatta 2400 gtccatgtgg ataaacgccc ccgcacagct agccctcact ttactgtttc aaaaatttct 2460 gttcctaaga cagaacatgg atatgaggca tcaatagccg gtagtgctat tgccacatta 2520 caaaaagagt tgtcagccac atcttctgct cagaagatca ccaaatcggt gaaggctcct 2580 actgtgaagc ccagtgagac tagagtaagg gcagagccca cacccttgcc acagttcccc 2640 ttcgctgaca caccagatac ttacaagagt gaagctggcg ttgaggtgaa aaaggaagta 2700 ggggtgagca tcactggcac caccgtccgt gaagagcgct ttgaagtact gcacggacgc 2760 gaagccaagg taacagaaac agcaagagta ccagcacctg ttgaaattcc tgttactcca 2820 ccaactttgg tctcgggctt aaaaaatgtg actgtcatag aaggtgaatc tgtcaccttg 2880 gagtgccaca tctctggata cccatccccg acagtgacat ggtacaggga agactaccaa 2940 atcgaaagtt ccattgactt ccagataacc ttccagagtg gaattgctcg tcttatgatt 3000 cgcgaagcat ttgcggaaga cagcgggcga tttacttgca gtgctgtaaa tgaggctgga 3060 accgtcagca catcctgcta tctggctgtg caggtgtcag aagaatttga aaaggaaacc 3120 acagccgtga ctgagaaatt tactacagaa gagaaacgct ttgttgagtc aagagatgtg 3180 gttatgactg atactagcct cacagaggaa caagcagggc ctggagaacc tgccgcgcct 3240 tactttatta caaaaccagt ggtccagaaa ctggtggaag gtgggagcgt ggtgtttgga 3300 tgccaagttg gcggcaaccc aaagccccat gtatactgga aaaaatctgg tgttcctcta 3360 accactggat acagatacaa agtgagttac aacaaacaaa ccggtgaatg caagctggtg 3420 atttctatga cttttgctga tgatgctgga gaatacacta ttgttgttcg caataagcat 3480 ggagaaactt ctgcatctgc ttccttgctt gaagaagctg attatgagtt actgatgaag 3540 tcccagcaag aaatgcttta tcagacacaa gtgactgcat ttgttcaaga acctgaagtt 3600 ggagaaacag cacctggatt tgtatactct gagtatgaaa aagagtatga aaaagaacaa 3660 gccttaatta ggaagaaaat ggccaaagat actgtagtgg tcagaactta tgtagaagat 3720 caggaattcc atatttcttc ctttgaagag agacttatta aagaaattga atatagaata 3780 ataaagacta cattagaaga acttcttgaa gaagatggag aagaaaagat ggcagttgac 3840 atttctgaat ctgaagctgt tgaatcagga tttgatttaa gaatcaagaa ttatagaatt 3900 cttgagggga tgggtgtcac ttttcattgc aagatgtctg gatatccatt accaaagatt 3960 gcttggtaca aagatggcaa gcgcatcaaa catggagaaa gataccaaat ggactttcta 4020 caagatggca gagctagtct gcgtatacct gttgttcttc cagaagatga aggaatctac 4080 actgcatttg ccagcaatat taaaggaaat gcaatttgct cagggaaatt gtatgtggag 4140 cctgctgcac cacttggagc tccgacttac attcccacac tagagccagt gagcagaatc 4200 agatctctct ctccacgttc agtgagcagg tctcctatac gcatgtctcc tgcacggatg 4260 tcacctgcaa ggatgtctcc tgcacggatg tcccctgcaa gaatgtcccc tggacgtagg 4320 ctggaggaga cagatgagtc acaacttgag agactatata aaccagtctt tgtgttaaaa 4380 cctgtttctt tcaaatgttt agaagggcaa actgccagat ttgacttaaa ggttgttggt 4440 agacctatgc cagagacgtt ctggtttcat gatggccagc aaattgtcaa tgactatacc 4500 cataaagtag tcattaaaga agatggtact caatcactaa ttattgtccc tgccacaccc 4560 agtgattctg gggaatggac tgtggttgcc caaaacaggg caggcagatc ttcaatttca 4620 gtgattttaa ctgtggaagc tgtggaacat caggtaaaac cgatgtttgt agaaaaactg 4680 aaaaatgtca atataaagga aggttcccga cttgaaatga aagtcagagc tacgggtaac 4740 cccaaccctg acattgtatg gttgaaaaac agtgacatca ttgtgcctca taaatatccc 4800 aaaatcagaa ttgaaggaac caagggagaa gctgccctta aaatcgattc cactgtcagc 4860 caagattctg cctggtatac tgcgactgct attaataaag ctggcagaga cactacaaga 4920 tgcaaagtaa atgttgaagt tgagtttgca gagcctgagc cagagagaaa gttaatcatc 4980 ccacggggga catatagagc aaaggagatt gcagccccag aactggagcc cctccatttg 5040 cgatatggcc aagagcaatg ggaagaaggt gatctctatg acaaagagaa acaacagaaa 5100 ccatttttca agaaaaaact cacttcctta agacttaagc gctttgggcc tgcccacttt 5160 gaatgcaggc taacacccat tagtgaccca acgatggtgg tggagtggct ccatgatgga 5220 aagccacttg aagcagccaa caggctccgt atgatcaatg aatttgggta ctgcagcctt 5280 gattatggcg ttgcatattc tagagacagt ggtatcatta cttgcagagc cactaacaaa 5340 tatggaacag atcacacatc tgctaccctt attgttaaag atgagaaaag tcttgtggaa 5400 gaatcccaat tgcctgaggg gaggaaaggc ttacagagaa ttgaagaatt agagagaatg 5460 gctcatgaag gtgcacttac aggtgtaaca acagatcaga aagaaaagca aaagccagac 5520 attgtcttgt acccagagcc agttagagta cttgaagggg agactgcaag gttccgctgc 5580 agggtaacag gctaccctca gcccaaagtc aactggtacc tcaatggaca gctcatccgc 5640 aaaagcaaaa ggttcagagt tcgctatgat ggtatccatt acctggacat cgtggactgc 5700 aaatcatatg acacaggtga agtgaaggtc accgcggaaa atcctgaagg tgtgatagag 5760 cataaagtga agcttgagat tcaacagagg gaagatttta ggtctgtcct taggagagct 5820 cctgaaccaa ggcctgagtt tcacgtacat gaaccaggaa agcttcagtt tgaagtacaa 5880 aaagtggata gacctgttga caccactgaa accaaagaag ttgtgaagtt gaaaagggct 5940 gaaagaatta cccatgaaaa agtgcctgaa gagtcggaag agctgcgcag taaattcaag 6000 cgcagaacag aagagggcta ttatgaagcc attaccgctg tggagctcaa gtctcgaaag 6060 aaggatgaat cctatgagga actcctcagg aagacaaaag atgaacttct ccactggacc 6120 aaagagttaa ctgaagagga aaagaaagct cttgccgaag aaggcaaaat cacgattcca 6180 acttttaaac ctgacaagat tgaactaagt cctagtatgg aggctccaaa aatcttcgaa 6240 agaatccaga gccaaacagt gggccaagga tctgatgcac acttccgggt cagagtcgtg 6300 gggaaaccag accccgaatg tgaatggtac aaaaatggtg tcaaaattga acggtctgac 6360 cggatctact ggtactggcc cgaagacaat gtttgtgaat tggtcataag agatgtgact 6420 gctgaggact ctgccagcat catggtaaaa gccatcaaca tagctggaga aacctccagt 6480 cacgcattct tacttgtcca agccaagcaa ttgatcactt tcacacagga attacaagat 6540 gttgttgcta aggaaaaaga cactatggca acctttgaat gtgaaacttc agaaccattt 6600 gtcaaagtga aatggtataa agatggtatg gaggttcatg agggagataa atacaggatg 6660 cactctgaca gaaaggttca cttcctctcc atactgacca ttgatacgtc tgatgctgaa 6720 gattacagct gtgtacttgt ggaagatgaa aatgtcaaaa cgactgctaa acttattgtt 6780 gaaggtgcag ttgttgagtt tgtgaaagaa cttcaggaca tagaagttcc agaatcatat 6840 tcaggagaat tagagtgcat tgtatcccca gaaaatatag aaggaaaatg gtatcataat 6900 gatgtggagc ttaaatccaa tggcaaatat acaattacat ctcgtcgtgg acgtcagaac 6960 ctcacggtca aggatgtaac caaggaggac cagggagaat acagctttgt catcgacggg 7020 aaaaagacaa cctgtaaatt aaagatgaaa ccccgcccca ttgctatcct acaaggactt 7080 agtgaccaaa aagtctgtga gggtgacatt gttcagcttg aagttaaagt ctccttggaa 7140 agtgtggaag gcgtctggat gaaagacggc caagaagtgc agcccagtga cagggttcac 7200 attgtgatag acaaacaatc tcatatgctg ctcattgaag acatgactaa ggaagatgct 7260 ggaaattact ctttcaccat tccagccctt ggcctctcca ccagtgggcg tgtctctgtc 7320 tatagtgtgg acgtgataac acctctaaaa gatgttaatg tgattgaagg caccaaggct 7380 gtgcttgaat gtaaggtgtc agtccctgat gtgacttctg ttaagtggta cttaaatgat 7440 gaacaaatca agcctgatga ccgtgtacag gccattgtga aaggtactaa acagcgacta 7500 gtcattaacc gaactcatgc ttcagacgaa ggaccttata agctgatagt tggcagagtt 7560 gaaaccaact gtaatctctc tgtagaaaaa attaaaatta tcagaggtct tcgtgacctt 7620 acctgtacag aaactcaaaa tgtggtgttt gaggttgagc tgtcccactc tggaattgat 7680 gtcctgtgga attttaagga caaggaaatc aagcccagtt ctaaatataa aattgaagca 7740 catggaaaaa tatataaatt gacagttcta aatatgatga aagatgatga aggaaaatac 7800 acattttacg cgggagaaaa tatgacatct ggaaaactta ctgtggcagg tggggccatc 7860 tccaagccac tcacagatca gaccgtagct gaatcccagg aagctgtgtt tgaatgtgaa 7920 gttgccaacc cagattccaa aggcgaatgg ttgagggatg gcaaacacct accactgact 7980 aacaacatca gaagtgagtc tgatggccac aaaaggagac ttatcattgc tgccaccaaa 8040 ttagatgaca ttggagaata tacctacaag gtggccacct ccaaaacatc tgccaaactc 8100 aaagttgaag ctgtcaaaat taagaagact ctgaagaacc tcacagtgac agaaacacag 8160 gatgctgttt tcactgtcga gcttacacac cctaatgtca aaggtgtcca gtggatcaaa 8220 aatggagttg tgctggaatc caatgaaaag tatgctatct ctgtcaaagg aacaatttac 8280 tctctgagga ttaaaaactg tgccatcgtg gatgagtctg tttatggctt caggcttgga 8340 aggcttggag ccagtgccag actgcacgtg gagactgtca agatcattaa aaagccaaag 8400 gatgtgacag ccttggaaaa tgccactgtt gcctttgaag ttagtgtttc ccatgacact 8460 gttccagtaa aatggttcca taagagtgtg gaaattaagc caagtgacaa acacagactg 8520 gtctcagaaa ggaaagtcca caagctgatg ctgcagaaca tctccccctc agatgctggg 8580 gaatacacag ctgtggtcgg gcaattggaa tgcaaagcaa aactgtttgt ggagacatta 8640 catattacaa aaaccatgaa aaatatcgag gtgcctgaga ccaaaactgc ctcttttgag 8700 tgtgaggtgt cccacttcaa tgtcccttcc atgtggctga agaatggtgt ggaaattgag 8760 atgagtgaaa agttcaagat agttgtgcag ggaaaactcc atcagctgat catcatgaac 8820 accagcacag aggactcggc agaatacaca tttgtctgtg gcaatgacca agtcagtgcc 8880 accctgacag tcactccaat catgattact tccatgctga aagacatcaa cgctgaagaa 8940 aaagacacta ttacttttga ggtgacagtg aactatgaag gcatctctta caaatggtta 9000 aagaatggtg tggaaatcaa atcaactgac aagtgccaga tgagaaccaa aaagctcaca 9060 cactcactga acatcaggaa tgttcacttt ggggatgctg ctgactacac ctttgtggct 9120 ggaaaagcaa catcaacagc cacactttat gtggaagctc gtcatataga atttaggaaa 9180 cacattaagg acattaaggt actggagaag aagcgagcca tgtttgaatg tgaagtttct 9240 gaacctgaca tcactgtaca gtggatgaaa gatgaccagg aactgcagat cacagacaga 9300 ataaagattc agaaggagaa atatgtccac cgccttctga tcccatccac ccggatgtct 9360 gatgctggga agtacacagt ggtggcagga ggcaacgtgt caactgcaaa actctttgta 9420 gaaggcagag atgttcgcat ccgaagtatt aaaaaggagg ttcaggtcat tgagaaacag 9480 cgtgctgttg ttgaatttga ggtcaatgaa gacgatgttg atgcccactg gtataaagat 9540 ggcattgaaa tcaatttcca agttcaagaa cgacacaaat atgtagtgga aagaagaatc 9600 caccgaatgt ttatctctga gaccagacag agcgatgcag gagaatacac ctttgtggca 9660 ggaaggaaca ggagttctgt cactctctat gtcaatgctc ctgaaccgcc ccaagttctg 9720 caggagctcc agcctgtcac tgtgcagtct ggcaagcctg cccgcttctg tgccatgata 9780 tccggaagac cacagcccaa aatttcctgg tacaaggaag agcagctgct ttccactggc 9840 ttcaagtgca aatttcttca tgatgggcaa gagtacacgc ttttgctaat tgaagccttc 9900 ccagaggatg cggcagtcta tacctgtgaa gccaagaatg actatggtgt tgccacaaca 9960 tcagcttcac tctcagtgga agttccagaa gttgtgtctc ctgatcagga aatgcctgtt 10020 tatccacctg ccatcatcac cccgcttcag gacactgtca cttctgaagg gcagccagcc 10080 cgttttcaat gccgggtttc tggaacagat ctaaaagtgt cgtggtacag caaagacaag 10140 aaaatcaagc catctcggtt ctttagaatg actcaatttg aagacactta tcaactggaa 10200 attgccgaag cttatccaga agatgaagga acttacacgt ttgttgctaa taatgctgta 10260 ggccaagtat caagcacagc caacctgagt ctggaagctc ctgaatcaat tttgcatgag 10320 aggattgaac aagagattga gatggaaatg aaagagtttt ctagttcttt tctgtctgcc 10380 gaggaagaag gacttcatag cgccgaactt caattatcta aaataaatga aacacttgaa 10440 cttttgtctg aatctccagt ttacccaact aaatttgatt ccgaaaagga aggcactggc 10500 ccaattttca tcaaagaagt gtcaaatgct gatataagca tgggggatgt ggctacactg 10560 tctgtaactg tcattggcat ccccaaacct aaaattcagt ggttctttaa tggagtgcta 10620 ttaacccctt ctgctgacta caaatttgtt tttgacggtg atgatcatag cctgatcatt 10680 ctgttcacca aattggagga tgagggagag tatacatgta tggccagtaa tgactatgga 10740 aagacaatat gtagtgccta tctaaaaatt aattccaaag gagagggtca caaagacact 10800 gaaacagaat cagcagtggc aaaatctctg gaaaagctgg gaggtccttg tcctcctcac 10860 ttccttaagg agttaaaacc aattcgctgt gctcaagggc ttcctgccat ctttgagtac 10920 acagtggttg gagagcctgc ccctactgtt acatggttca aagaaaacaa gcagctttgc 10980 accagtgttt attacactat cattcataac cctaatggct ctggaacttt cattgtcaat 11040 gaccctcaga gggaagacag tggcctctat atctgtaaag cagagaatat gttgggtgag 11100 tccacctgtg cagcagagct gcttgtgctt ctggaagaca cagacatgac tgataccccc 11160 tgcaaagcaa agtccacacc agaggctcct gaggattttc cacagacacc cttaaagggt 11220 cccgcagttg aagcacttga ctcggagcag gaaattgcaa cgtttgtaaa agacaccatt 11280 ttgaaagctg ctttaattac agaagaaaac cagcaactat cttatgagca tattgctaaa 11340 gccaatgaat tgagcagtca gcttcctttg ggagctcagg aattgcaatc cattttggag 11400 caagacaagc tcactcctga aagcaccagg gaatttcttt gcatcaatgg cagtattcac 11460 tttcagcctc tcaaggaacc atctcccaac ctacagctgc agattgtaca gtcccagaaa 11520 accttctcca aagaaggtat tctaatgcct gaagagcctg agacacaggc agttctatca 11580 gataccgaga aaatcttccc aagtgccatg tccatagaac aaattaattc attaacagtt 11640 gagcctctga aaactttatt agctgaacct gaagggaatt atccacagtc ttcaatagaa 11700 cctccaatgc attcttatct aacctctgtg gctgaggaag tactttcact aaaagaaaag 11760 acagtatctg acaccaacag agagcaaaga gtgactcttc aaaagcaaga ggcacaaagt 11820 gcgctcatct tgagtcagag cttagctgag ggacacgtgg agagtctcca gagtcctgat 11880 gtcatgatct ctcaggtaaa ctatgagccc ctagtccctt cagaacactc atgcacagaa 11940 ggaggtaaaa ttttgataga aagtgcaaat ccactggaaa atgcagggca agattctgcg 12000 gtcagaattg aggaaggcaa gtccttaaga tttccactag cacttgaaga aaagcaggta 12060 ctgctcaaag aagagcattc tgacaacgtg gtgatgcccc cagaccaaat cattgagtct 12120 aaaagagagc ccgtggcaat aaagaaagtg caggaggtac agggaaggga ccttctttct 12180 aaggaaagct tgctttctgg tattccagaa gagcagagat taaacctgaa aattcaaatc 12240 tgccgggctt tgcaagcagc cgtggccagc gagcagccag gtcttttctc tgagtggcta 12300 agaaatattg aaaaggtgga ggtcgaggct gtaaacatca cccaagagcc cagacacatc 12360 atgtgcatgt accttgttac ttcggcaaag tctgtaacag aagaagtaac catcattatt 12420 gaagatgttg atcctcaaat ggctaacctg aaaatggaac ttagggatgc tttgtgtgct 12480 attatatatg aggaaataga catcctaaca gctgagggtc ctagaattca gcaaggagcc 12540 aaaacaagtt tgcaagaaga aatggattct ttttcaggtt cacagaaggt tgaacccatt 12600 actgaaccag aagttgaatc taaatatctg atctcaactg aagaggtcag ttattttaac 12660 gtgcaaagta gggttaaata tttggatgcc acacctgtca ctaaaggggt tgcttcagct 12720 gttgtctctg acgaaaaaca agatgagagt ctgaaaccat cagaggaaaa agaggagtct 12780 tcctctgaaa gtggtactga ggaggttgct acagtaaaga tacaggaagc tgagggtggc 12840 ttaatcaaag aggatggccc catgatacat acacctttag tggacactgt ttctgaggaa 12900 ggtgatattg tacacctcac aacatccata acaaatgcta aagaggtgaa ttggtatttt 12960 gagaataaac tggtgccttc agatgaaaag ttcaagtgtt tacaagatca aaatacatat 13020 acgctagtca tcgacaaagt aaataccgaa gaccatcaag gagagtatgt ctgtgaggcc 13080 ttgaatgaca gcggaaaaac agcaacttca gccaaactca ctgtagtaaa aagagctgcc 13140 ccagtgatca agaggaaaat cgaacccctg gaagtagcac tgggccacct agccaaattc 13200 acctgtgaga tccaaagtgc tcccaatgtc cggttccagt ggtttaaagc tggccgagaa 13260 atttatgaga gtgacaagtg ttctattcga tcttcaaagt atatctccag ccttgaaatc 13320 ctgagaaccc aggtggttga ctgcggcgag tatacatgca aagcttccaa tgagtatggc 13380 agtgtcagct gtacagccac actaactgtg acagtgcctg gaggtgaaaa gaaagttcgc 13440 aaattacttc cggaacgtaa acctgaacca aaggaagaag ttgttctgaa aagcgttcta 13500 agaaaaagac ctgaagaaga agaacctaaa gtagaaccta aaaaactaga aaaagttaaa 13560 aaacctgcag taccagaacc accacctcca aaacctgttg aagaggttga agtacctact 13620 gttacaaaaa gggaaaggaa gattcctgaa ccaacaaaag tgcctgaaat caagccagca 13680 atacctctcc ctgcacctga accgaaacca aagcccgaag cagaagtgaa aacaatcaaa 13740 ccacctcctg tggaacctga accaaccccc atcgctgccc cagtaacagt gccagtggtt 13800 ggaaagaaag cagaagccaa agcacctaag gaagaggctg ccaagccaaa aggtcctatc 13860 aaaggtgtac ccaaaaagac tccttcacca atagaagccg aaaggagaaa gttaaggcca 13920 ggaagtggtg gagagaaacc tcctgatgaa gccccgttca cctaccagct aaaggctgtg 13980 ccactgaagt ttgtgaaaga aatcaaagac atcatcttga cagaatcaga gttcgttggc 14040 tcttcagcaa tctttgaatg tttggtctcc ccttccactg caattacaac ctggatgaaa 14100 gacggtagca atatccgtga gagtcccaag cacaggttta ttgcagatgg taaagacaga 14160 aagctgcaca tcattgatgt tcaactttcc gatgctggtg aatacacctg tgttttacgt 14220 ttgggaaaca aagaaaagac ctccacggct aaacttgttg tagaagaact tcctgtgcgt 14280 tttgtaaaaa cactggaaga ggaagtcaca gtggtcaaag gacagccatt gtacttgagc 14340 tgcgagttaa acaaagagcg tgacgtggtc tggaggaagg atggcaagat tgtggtggag 14400 aaacctggcc gaattgtgcc aggcgtcatt ggcttgatgc gggctctgac catcaacgat 14460 gcagatgaca cagatgctgg aacatacaca gttactgtgg aaaacgccaa caacctggag 14520 tgttcatctt gcgtaaaagt agtagaagtc attagagatt ggctggtgaa acctatacga 14580 gaccagcatg tgaaacccaa ggggacagct atttttgcct gtgatatagc aaaagatact 14640 ccaaacatta agtggttcaa aggatatgat gaaatccctg cggaaccaaa tgataagact 14700 gaaatactga gagatggaaa tcatctgtac ctcaaaatta agaatgctat gccagaagat 14760 attgctgagt atgcagtgga aattgaagga aaaagatacc ctgcaaagct gacacttgga 14820 gagcgtgaag ttgaactgct taaaccaata gaggacgtta ccatttatga gaaagaaagt 14880 gcaagctttg atgcagaaat ctcagaggca gacattcctg gacaatggaa actgaaagga 14940 gaacttctaa ggccctcacc tacttgtgaa atcaaagcag aaggtggaaa acgcttctta 15000 actttgcaca aagtcaaact ggaccaagct ggtgaagtcc tctaccaggc ccttaatgca 15060 attacaactg ccattttgac agtaaaagaa atcgaacttg actttgctgt gcccctgaag 15120 gatgtcactg ttccagaaag gcgacaggct cgattcgaat gtgtcctcac ccgagaggca 15180 aatgttatat ggtccaaagg acctgatata attaagtcat ctgacaaatt tgatatcatc 15240 gctgatggaa agaaacatat tcttgttatt aatgattctc aatttgatga tgaaggggtc 15300 tatactgctg aggtggaggg caagaagacc tcagctcggt tgtttgtcac aggtataaga 15360 ctgaaattca tgtcacctct tgaagatcaa acagtaaaag aaggtgaaac agcaactttt 15420 gtttgtgaac tttctcatga aaaaatgcat gtagtctggt tcaaaaatga tgccaaactc 15480 catacaagca gaacagtact catctcttct gagggcaaga ctcacaaatt ggaaatgaaa 15540 gaagtgacat tggatgatat atctcagata aaagctcaag tcaaggagct gagctccaca 15600 gcacagctga aggtcttaga ggccgatccc tacttcactg tgaaattaca tgacaaaact 15660 gcagtggaga aggatgagat tactttgaag tgtgaagtga gcaaagatgt accagtgaaa 15720 tggttcaaag atggtgaaga gattgtccct tcacccaaat attctatcaa ggcagatggc 15780 ctgcgccgca tcttaaaaat caaaaaggcg gaccttaaag ataaaggcga atatgtgtgt 15840 gactgtggca cagacaagac caaggcaaat gttactgttg aggctcgact aatagaagtg 15900 gaaaagcctc tgtacggagt agaggtgttt gttggtgaaa cagcccactt tgaaattgaa 15960 ctttctgaac ctgatgttca cggccagtgg aagctgaaag gacagccttt gacagcttcc 16020 cctgactgtg aaatcattga ggatggaaag aagcatattc tgatccttca taactgtcag 16080 ctgggtatga caggagaggt ttccttccag gctgctaatg ccaaatctgc agccaatctg 16140 aaagtgaaag aattgcctct tatcttcatc acacctctca gtgatgttaa agtcttcgag 16200 aaagatgagg ctaagtttga gtgtgaagta tccagggagc ccaaaacatt ccgttggcta 16260 aaaggaaccc aggaaatcac aggtgatgac agatttgagc ttataaagga tggcactaag 16320 cattcaatgg tgatcaagtc agctgctttt gaagatgaag caaaatacat gtttgaagct 16380 gaagataagc acacaagtgg caaactgatc attgaaggaa tccggctcaa attcctcacc 16440 cctctcaaag atgtaactgc caaagagaag gaaagtgctg tatttactgt ggagttatct 16500 catgataaca tccgagttaa atggttcaag aatgaccagc gcctacacac caccaggtcg 16560 gtctcaatgc aagacgaagg gaaaactcat tcgatcacat tcaaagacct gtctattgat 16620 gacacctccc aaattagagt agaagctatg gggatgagtt cagaagctaa actcactgtg 16680 cttgagggag acccatattt tacaggaaaa cttcaagatt atactggtgt agagaaagat 16740 gaagttattc tacagtgtga aattagcaaa gcagatgcac cagtgaaatg gtttaaggat 16800 gggaaggaaa taaagccatc caaaaatgct gttattaaga cagatggcaa gaaacgcatg 16860 ctaatcctaa agaaagcctt gaaatcagat attggacagt acacctgtga ctgtgggaca 16920 gataagacct caggaaaact tgacattgag gatcgggaaa ttaaactggt gcgacccctg 16980 cacagtgtgg aggtgatgga gactgagaca gcacgctttg aaaccgaaat ctctgaagat 17040 gatatccacg ccaactggaa actcaaggga gaggccctac tccaaacacc tgattgtgaa 17100 attaaggaag aaggcaaaat acactccctt gttttgcaca actgtcgcct ggaccagacg 17160 ggtggggtgg atttccaagc tgccaatgtt aaatctagtg cccacctccg agttaagcca 17220 cgagtaattg gtcttctgag gcctttaaag gatgtcaccg tgactgcagg ggaaacagcc 17280 accttcgact gcgagctctc ctacgaagat atcccagtgg aatggtatct caaagggaag 17340 aaactagagc ccagcgataa ggtggtccca cgttcagaag gaaaagttca tacacttact 17400 ctgagggatg taaagttaga agatgctggg gaagtccaac taacagcaaa agatttcaaa 17460 actcacgcca acctctttgt gaaagaaccc ccagttgaat tcactaagcc tcttgaggac 17520 cagacggtcg aagagggagc cactgcagtg ctggagtgtg aagtctccag agaaaatgct 17580 aaggtgaaat ggttcaaaaa tgggacagaa atcctcaaaa gcaagaagta tgaaattgtt 17640 gctgatggca gggtcagaaa acttgttata catgactgta ccccagagga tattaaaaca 17700 tacacttgtg atgctaagga ttttaagact tcctgtaacc tgaatgtcgt gcctcctcat 17760 gtggaattct taagaccact caccgacctt caagttagag aaaaagaaat ggctcgattt 17820 gagtgtgaac tttcccgaga aaatgctaag gttaagtggt ttaaagatgg tgctgaaatt 17880 aaaaagggca aaaaatatga catcatatcc aagggagcag tgcgcattct tgtcatcaac 17940 aaatgtctac tggatgatga agctgaatat tcctgtgaag taaggacagc gagaacttct 18000 ggcatgctga cagttctgga agaagaagct gtctttacca aaaatcttgc caacattgaa 18060 gttagtgaaa cagacactat aaaactggtt tgtgaagtct ccaaacctgg cgcagaagtg 18120 atttggtata aaggggatga ggagatcatt gaaacaggaa gatatgaaat actgactgaa 18180 ggacggaaga gaatcctggt cattcagaac gctcaccttg aggatgctgg caactacaac 18240 tgtcgactcc caagctctcg aaccgatggc aaagtcaaag tacatgaact ggctgctgaa 18300 tttatctcaa agcctcaaaa ccttgaaata cttgaaggag aaaaggctga atttgtctgc 18360 tctatatcaa aagaaagctt tccagtccag tggaagaggg atgataagac acttgaatct 18420 ggagataaat atgacgttat tgctgatggt aaaaagaggg tcctagttgt gaaagatgcc 18480 acattacaag atatgggcac ttacgttgtc atggtagggg ccgccagagc agcagctcac 18540 ttgacagtca ttgaaaaact caggatcgta gttcctctta aggacacccg ggtgaaggaa 18600 caacaggaag ttgtcttcaa ctgtgaagtc aatactgaag gtgccaaagc caaatggttc 18660 agaaatgaag aagctatatt tgatagttca aaatacatca ttctccaaaa agacctagtc 18720 tacaccctca gaattagaga tgcacactta gatgaccaag ccaactataa tgtgtctttg 18780 accaatcaca gaggtgaaaa tgttaaaagt gcagccaatc taatagtaga agaggaagac 18840 cttaggattg ttgagcctct taaagatatt gaaacaatgg agaagaaatc tgtcacattc 18900 tggtgcaagg tgaatcgtct caatgtaaca ctgaagtgga ccaaaaatgg tgaagaagtg 18960 ccttttgaca accgtgtctc atacagagtt gataagtaca agcacatgtt aaccattaaa 19020 gactgtggct tcccagatga aggtgaatac attgtcactg ctggacaaga taaatctgtt 19080 gctgagcttc tcatcataga agccccgaca gaatttgtgg aacacttgga agatcagaca 19140 gtcactgagt tcgatgacgc tgtcttctcc tgccagctct ccagagagaa agccaatgta 19200 aaatggtaca gaaatgggag agaaatcaaa gaaggcaaaa aatacaaatt tgaaaaagat 19260 ggaagtatac acagactcat tataaaagat tgcaggctgg atgatgagtg tgaatatgct 19320 tgcggggtag aagacaggaa gtctcgtgct agactttttg tggaagaaat tcctgttgag 19380 atcatcaggc ctccacaaga tattcttgaa gcccctggtg ctgatgttgt ctttttagca 19440 gaactcaata aagataaggt ggaagtccaa tggctaagaa ataacatggt tgttgtccag 19500 ggtgataaac accagatgat gagtgaagga aagatacatc gactacagat ttgtgatatt 19560 aagccccgtg accagggtga atacagattt attgccaaag acaaagaagc cagagctaag 19620 cttgaactgg cagctgcacc aaaaatcaag acagctgacc aagaccttgt ggttgatgtt 19680 ggcaagcctc tgacaatggt ggtgccatat gatgcctacc ccaaagcaga agctgaatgg 19740 tttaaagaaa atgaaccttt atctacaaaa accattgata ctacggctga acaaacttct 19800 ttcagaattt tagaagccaa gaaaggagac aaagggaggt ataaaattgt gcttcagaac 19860 aaacatggaa aagcagaagg attcatcaat ttaaaagtta ttgatgttcc tgggccagta 19920 cgtaacttag aagtgacaga aacatttgat ggtgaagtga gccttgcttg ggaagaacct 19980 ttaactgatg gtggaagcaa aatcataggt tacgttgttg aaagacgtga cattaagaga 20040 aagacctggg ttctggccac agaccgtgca gagagttgtg agtttactgt cactggtcta 20100 cagaaaggag gagttgagta cctattccgt gtgagtgcaa gaaacagagt tggcactggt 20160 gagccagtag aaactgacaa tcctgtagaa gcaaggagta aatatgatgt tccaggccct 20220 cctttgaatg taaccatcac tgatgtgaat cgatttggtg tctcactgac atgggaacca 20280 ccagagtatg atggaggtgc tgagatcaca aactacgtca ttgaattaag agacaagact 20340 tctatcaggt gggatactgc catgactgtg agagctgaag acctgtctgc aactgttact 20400 gatgtggtag aaggacagga gtacagtttc cgagtgagag cccaaaatcg aattggagtt 20460 ggaaaaccaa gtgcagccac acccttcgtc aaagttgctg atccaattga gagaccaagt 20520 cctcctgtaa acctaacttc ctcagatcag actcagtcat cagttcagct caaatgggaa 20580 cctcctctga aagatggagg aagcccaata ttaggctata taattgagcg atgcgaagaa 20640 ggaaaagata attggattcg ttgcaatatg aaacttgtcc ctgaactgac ttacaaggtt 20700 accggattgg aaaaaggaaa taaatattta tatagagtat ctgcagaaaa taaagctggt 20760 gtttcagatc catctgaaat tcttggtcct ctcaccgctg acgatgcatt tgttgaacca 20820 acaatggatt taagtgcatt taaagatggt ctggaagtta ttgtcccaaa tcctatcacg 20880 atcctggttc caagtacagg ctatccaagg ccaactgcaa cctggtgttt tggagataaa 20940 gtactagaaa caggggaccg ggtgaaaatg aagaccttgt ctgcctatgc cgaacttgtc 21000 atttctccaa gtgaacgttc agacaagggc atttatacac tgaaattaga aaaccgtgtg 21060 aaaacaattt ctggggaaat tgatgtcaat gtaattgctc gcccaagtgc acccaaagaa 21120 ttgaaatttg gtgatataac caaggactca gtacatttga cttgggaacc acctgatgat 21180 gatggaggaa gtccgttaac tggatacgtt gttgaaaaac gagaagtcag ccggaaaaca 21240 tggactaaag ttatggactt tgtgactgat ctagaattca cagttcctga tcttgttcaa 21300 ggaaaagagt acttatttaa agtttgtgct cgtaacaaat gtggccctgg agaacctgca 21360 tatgttgatg aacctgtaaa tatgtcaact cctgcaacgg tacctgaccc accagagaat 21420 gttaaatgga gagatcgaac agccaatagc atcttcttaa catgggatcc acctaaaaat 21480 gatggtggtt cacgcatcaa aggatatata gttgaaagat gtccacgtgg ttctgataaa 21540 tgggttgcct gtggagaacc tgttgcagaa acaaaaatgg aagtgacagg tcttgaggaa 21600 ggcaaatggt atgcctaccg cgtgaagacc ttaaacaggc agggtgctag caaaccaagc 21660 agacccacag aggaaatcca ggctgtggac acacaagagg ccccagaaat cttcctcgat 21720 gtgaagctcc ttgctggtct cactgtaaaa gctgggacca agattgaact tcctgccacc 21780 gtaaccggaa aacctgaacc taaaataact tggacaaagg ctgatatgat tctgaagcag 21840 gacaaaagaa ttaccattga aaatgtccct aagaaatcca cagtgactat tgttgatagt 21900 aagagaagtg acactggcac atatatcatt gaggctgtga atgtgtgtgg ccgggccact 21960 gctgtggtgg aagtgaacgt cttagataaa cccggaccac cagctgcctt tgacatcaca 22020 gatgtaacca atgagtcatg tcttctaaca tggaacccac cacgcgatga tggtggatct 22080 aagatcacaa actatgttgt ggagagacga gcaactgata gtgaagtgtg gcacaagctc 22140 tcatccaccg tcaaggatac aaacttcaag gccaccaaat taatccccaa taaagagtac 22200 atcttcagag ttgctgcaga aaacatgtat ggtgctggtg aaccagttca ggcctctcca 22260 ataacagcca aatatcagtt tgatccacct ggtcctccaa ctcgcctaga accttctgat 22320 atcactaaag acgcagtgac tctcacatgg tgtgagccag atgatgatgg tggcagccca 22380 atcacaggat actgggttga aagactggat cctgatacag ataaatgggt tagatgcaat 22440 aagatgccag taaaggacac aacatacaga gtgaaaggtc tcactaataa gaaaaaatac 22500 agattccgtg tgttggctga aaatcttgct ggacctggaa aaccaagcaa atcaactgaa 22560 ccaatcttaa taaaggatcc catagatcct ccatggcccc ctggaaaacc aactgtaaaa 22620 gatgtaggca aaacatcagt aaggttgaat tggacaaaac cagaacatga tggaggtgca 22680 aagattgagt cttatgtcat tgaaatgctg aagactggaa cagatgagtg ggtcagagtg 22740 gcggaagggg ttcccaccac tcagcacttg ctcccagggc tcatggaagg acaggaatac 22800 tcattccgag ttagagctgt gaataaggct ggggaaagtg aacccagtga acccagtgac 22860 cctgtgcttt gccgggagaa gctatatcct ccatcaccac cacgctggct tgaagttatt 22920 aatatcacaa aaaatacagc agacctaaaa tggacagttc ctgagaaaga tggagggtcc 22980 cccatcacca actacattgt ggaaaagaga gacgtcaggc gaaaaggctg gcaaacagtg 23040 gataccactg tcaaggacac caagtgcaca gtcaccccac tgactgaggg ctctttatat 23100 gtgttccgag ttgctgcaga aaatgctata ggacaaagcg actacaccga aattgaggac 23160 tctgtgctgg ccaaagacac ctttaccact cctggaccac cctacgccct ggcagtggtt 23220 gatgtgacaa aacgacatgt tgacctaaag tgggagccac ctaaaaatga tggtggaaga 23280 ccaatacaga gatatgtcat tgagaagaaa gaaaggttag gtacccgttg ggtgaaagct 23340 ggaaagactg caggacctga ctgtaacttc agagtaactg atgtcatcga aggaacagag 23400 gtccagtttc aggttcgggc tgaaaatgaa gctggagttg gccacccaag tgaacccaca 23460 gaaatcctat ccattgaaga tccaacaagt cctccctcac caccccttga cctacatgtg 23520 actgatgctg ggagaaaaca cattgccatt gcttggaagc ctccagagaa aaatggtgga 23580 agtcctatca taggatacca tgttgaaatg tgtccagtag gcactgagaa atggatgaga 23640 gttaattctc gcccaataaa ggacttgaaa ttcaaggttg aagaaggtgt tgttcctgac 23700 aaagaatatg tcctgagagt gagagcagtc aatgctattg gtgtcagcga gccatctgaa 23760 atctctgaaa atgtggttgc caaagaccca gactgcaagc caacaattga cctggagact 23820 catgacatta ttgttattga aggtgaaaag ttaagcattc ctgttccctt cagagctgtc 23880 ccagttccaa ctgttagttg gcataaagat ggcaaagaag ttaaagcaag tgatagatta 23940 acaatgaaga atgatcacat ctctgcacac cttgaagttc ccaagagtgt ccgtgcagat 24000 gccggaattt ataccattac actggagaat aagctcggct cagcaacagc ctcaatcaat 24060 gtcaaagtca taggcctacc tggaccatgc aaagatatta aagcaagtga cattaccaag 24120 agttcttgta agttaacttg ggaacctcca gaatttgatg gtggaacccc aattcttcat 24180 tatgtcctgg agcgcagaga agctgggagg agaacatata taccagtcat gtctggtgag 24240 aacaaactgt catggactgt gaaggatctc ataccaaatg gtgaatactt cttccgtgtt 24300 aaagcagtca acaaggttgg tggaggagaa tatattgaac tgaaaaatcc agtcattgct 24360 caagatccaa agcaaccccc tgatccacct gtagatgtag aggttcataa tcctacagcg 24420 gaggcaatga ctattacatg gaagccacct ttgtatgatg gagggagcaa gataatgggc 24480 tacatcatag agaagattgc taagggtgaa gaaaggtgga agagatgcaa tgaacacctg 24540 gtaccaatcc tgacctatac agcaaaagga cttgaagagg ggaaagagta ccaattccgt 24600 gtgcgagcag agaacgccgc gggtattagt gaaccttctc gggctactcc tccaaccaaa 24660 gctgtagatc ccattgatgc ccccaaagtc attctgagaa caagcctaga agtgaaacga 24720 ggtgatgaaa tagcacttga tgcaagtatt tctggatcac cttacccaac tattacatgg 24780 ataaaggatg aaaatgttat tgtaccagag gaaattaaga agcgtgcagc acccttggtt 24840 aggagaagga agggtgaagt tcaagaagaa gaaccatttg tcctgcctct gacacagcgt 24900 ttgagtattg acaacagcaa aaagggagaa tctcagctac gcgtccgaga ttctctccga 24960 cctgaccatg gtctgtatat gatcaaagtt gaaaatgacc acggtattgc aaaagctcct 25020 tgtactgtca gtgtgttaga tacaccggga ccaccaatca actttgtatt tgaagatatc 25080 agaaagacct cagtcctttg taaatgggaa ccaccccttg atgatggtgg cagtgaaatc 25140 ataaactaca ctttggaaaa gaaagacaag acaaaacccg actcagaatg gattgttgtc 25200 acttcaacac ttagacattg caaatattca gtaacaaaac tgattgaagg aaaagagtac 25260 ctcttccgtg taagagctga aaacagattt gggccaggtc caccatgtgt ttcaaagcca 25320 cttgtggcta aagatccatt tggaccacct gatgcaccag ataagcccat tgtggaagat 25380 gttaccagca acagtatgct agtgaaatgg aatgaaccaa aagataatgg aagccccatt 25440 ttgggttact ggcttgaaaa acgtgaagtt aacagtacac attggtctcg tgtcaacaaa 25500 agccttctga atgccttgaa agccaatgta gatggcttat tagaaggact cacctatgtc 25560 ttcagagtat gtgctgaaaa tgcagctgga cctggaaagt tcagtccacc ttcagatccc 25620 aaaacagcac atgatccaat ctctcctcct gggccaccta tcccaagagt cactgacaca 25680 agctctacaa ctattgaact agaatgggaa cccccagctt tcaatggtgg tggggaaatt 25740 gttggctatt ttgttgataa gcagttggtt ggcacaaata aatggtcacg ctgcacagag 25800 aagatgatca aggtccgtca gtacaccgtc aaagaaatcc gagagggtgc tgattacaaa 25860 cttcgggtga gtgctgtcaa tgccgcaggg gaaggaccgc ctggagaaac acaacctgtt 25920 actgtggctg aaccacaaga gcctccagct gtggaactgg atgtttctgt caagggtgga 25980 atacaaataa tggctgggaa gactcttaga attccagctg tggtgactgg tcgccctgta 26040 cctacaaaag tatggaccaa agaagaaggg gagctggata aagaccgtgt tgtaatagac 26100 aacgttggaa ccaaatctga actaattatc aaggatgcac tgcgaaaaga ccatggcaga 26160 tatgtgatta cagctacaaa tagctgtggt tccaaatttg cagcagccag ggtagaagtt 26220 tttgatgtcc ctggtccagt tcttgactta aaacctgttg taacaaacag aaaaatgtgt 26280 ctacttaact ggtctgatcc agaagatgat ggaggaagtg aaataacagg ctttatcatt 26340 gaaagaaaag atgccaagat gcatacttgg agacaaccaa tagagactga gagatctaaa 26400 tgtgacatca caggtctgct tgagggacaa gaatataagt tccgtgttat tgccaagaac 26460 aagtttggct gtggccctcc tgttgaaata ggaccaattc ttgcagttga tccactaggt 26520 cctccaacat ctccagagag gctcacatac actgaaagac aaaggtccac tatcacactt 26580 gactggaaag agccccgcag taatggtggc agtcccatcc aaggatatat cattgaaaaa 26640 cggcgtcatg acaaacctga ctttgaaaga gttaacaagc gactctgccc aaccacatct 26700 tttctggttg aaaatcttga tgaacaccaa atgtatgagt tccgtgtcaa agctgtcaat 26760 gaaattggtg aaagtgaacc atccctacct cttaatgtag tcatacaaga tgatgaagtg 26820 cctccaacta ttaagttgcg tctgagtgtt cgaggagaca ctatcaaagt taaggcagga 26880 gagcctgtcc acatccctgc agatgtgaca ggccttccaa tgcctaagat tgaatggtcc 26940 aaaaatgaaa ctgtaattga aaaacccact gatgcacttc agataaccaa ggaagaggta 27000 tcccgaagtg aggcaaaaac tgagcttagc attcccaaag cggtccggga ggacaaaggc 27060 acttacacag ttactgcttc caatcgcctt ggctcagtgt tccgaaatgt tcacgttgaa 27120 gtatatgacc gcccatcccc accaagaaat cttgctgtta ctgacattaa agctgaatct 27180 tgctacttga catgggatgc ccctcttgat aatggtggca gtgaaatcac ccattatgtt 27240 attgacaaac gtgatgcaag taggaagaaa gcagaatggg aggaagtcac caacactgct 27300 gtagagaaaa gatatgggat ctggaaactt atccccaatg gtcagtatga gttccgagtc 27360 agggcagtga ataaatatgg aatcagtgat gagtgcaaat cagataaagt agtcattcaa 27420 gatccttatc gccttcctgg acctccagga aaaccaaaag ttttggcacg caccaaagga 27480 tcaatgctag tgagctggac tcctcctttg gacaatggtg gctctccaat tactggctac 27540 tggctggaga aaagagaaga gggaagtcct tattggtcac gtgttagccg agcaccaata 27600 accaaagtgg gattgaaagg cgtggaattt aatgttcctc gtttgcttga aggcgttaaa 27660 taccagttca gagccatggc aataaatgct gcaggaattg gtcctcccag tgaaccatca 27720 gatccagagg ttgcaggaga tcccatattt ccaccggggc caccttcttg cccagaagtt 27780 aaagataaaa cgaagtcaag catctcacta ggatggaaac ctccagccaa agatggtggc 27840 agcccaatca aaggatacat tgtagaaatg caagaagaag gtactactga ctggaaaaga 27900 gtaaatgaac cagacaaact tataactacc tgtgaatgtg tggtgcctaa tctgaaagag 27960 ctcaggaagt acagattcag agtgaaagct gtcaatgaag ctggtgaatc tgaaccaagt 28020 gatacaactg gggagatccc tgccactgat attcaagagg aaccagaagt tttcattgac 28080 attggagcac aggactgtct ggtttgtaaa gctggctcac agattaggat tcctgctgtc 28140 atcaagggac gcccaacacc aaaatcatct tgggaatttg atggaaaggc aaagaaagca 28200 atgaaggatg gagttcatga catacccgaa gatgcacagc tggagactgc tgaaaactcc 28260 tcagtaatta ttattccgga gtgtaaacga tctcatacag gcaaatacag catcacagcc 28320 aagaataaag caggacaaaa gactgcaaat tgcagagtta aagtcatgga tgtaccaggc 28380 ccacccaaag atctgaaagt cagtgatatc acaaggggta gttgcagact ttcatggaag 28440 atgccagacg acgatggagg agacaggatc aaaggctatg ttattgagaa gaggactatt 28500 gatggaaaag cctggaccaa agtcaatcca gactgtggaa gcaccacatt tgtagtgcct 28560 gatctcctct ctgaacagca atatttcttc cgtgtgcgag cagaaaaccg ttttggtatt 28620 ggcccacctg tggaaaccat tcagaggacc actgccagag atccgatata tcctcctgat 28680 cctcctatta aactcaagat tggcctcatc acaaagaaca cagtgcatct gtcatggaaa 28740 cccccgaaga atgatggggg ctcccctgtt acccactata ttgttgagtg ccttgcatgg 28800 gaccctactg ggacaaagaa agaagcctgg aggcagtgca ataagcgtga tgtggaagaa 28860 ctgcaattta ctgttgaaga cctagtagaa ggtggggaat atgaattccg agtcaaagct 28920 gtcaatgctg caggagtcag caagccttca gccactgttg gcccctgtga ctgtcaaaga 28980 ccagacatgc caccatcaat tgatctaaaa gaattcatgg aggttgaaga aggaaccaat 29040 gttaacattg tggccaaaat taaaggtgtg ccattcccga cactaacctg gtttaaagct 29100 cctccaaaga agcctgataa caaagaacct gttctctatg acacccatgt caacaaactg 29160 gtggtagatg atacttgcac tttagttatt ccgcagtctc gcaggagtga cactggctta 29220 tataccatca cagctgtaaa taatctggga acagcatcaa aggagatgag actgaatgtc 29280 ctgggtcgtc ctggccctcc agtgggaccc ataaaatttg aatctgtttc agcagatcaa 29340 atgacactat cttggtttcc acctaaagat gatggtgggt ctaagattac aaactatgta 29400 attgagaaaa gagaagctaa caggaagaca tgggtccatg tctccagtga acctaaggag 29460 tgcacgtaca cgattcccaa attgctagaa ggccatgaat atgtattccg aatcatggcc 29520 cagaataaat atggcattgg agaacctctt gacagtgaac ctgaaacagc aagaaacctc 29580 ttctctgtcc ctggagcacc agataaacca acagttagca gcgtgactcg taactccatg 29640 actgtcaact gggaagagcc agaatatgat ggaggctctc ctgtgacagg gtactggctg 29700 gaaatgaaag acaccacttc aaagagatgg aagagagtta accgagatcc tatcaaagcc 29760 atgactttgg gtgtttctta taaagtgact ggtcttattg aaggttccga ctatcaattc 29820 cgggtatatg caatcaatgc tgctggcgtg ggtccagcaa gtctgccatc agacccagcg 29880 actgctagag atccaattgc ccctcctggt cctccatttc ccaaagtgac agattggact 29940 aaatcatctg cagatctgga gtggtctccc ccactaaaag atggtggatc caaagtaact 30000 ggatacatcg ttgaatataa agaagaagga aaagaagaat gggaaaaggg taaagataaa 30060 gaagtgagag gaacaaagct cgttgtgaca ggattaaagg aaggagcatt ctacaaattt 30120 agagttagtg cagtcaacat tgctggcatt ggagaacctg gagaggtcac agatgtcatt 30180 gaaatgaagg acagacttgt ttcacctgac cttcagctag atgccagtgt cagagataga 30240 attgttgtcc atgctggagg ggtgatccga atcattgcct atgtgtctgg aaagcctcct 30300 ccaaccgtca cctggaacat gaatgaaaga accttacctc aagaagccac cattgagacc 30360 acagccatta gctcatccat ggtcatcaag aactgccaga ggagccatca aggcgtctat 30420 tctcttcttg ccaaaaatga agccggagaa agaaagaaga caattattgt tgatgtatta 30480 gatgttccag gtcccgttgg aacaccattc ctagctcaca acctaaccaa tgagtcctgc 30540 aaactgacat ggttttctcc agaagatgat ggaggctctc caatcaccaa ttatgtcatt 30600 gaaaagcgtg aatctgaccg cagagcatgg accccagtga catatacagt tacccgacaa 30660 aatgctactg tccagggtct cattcaagga aaagcctact ttttccgaat tgcggctgaa 30720 aatagtattg gcatgggtcc atttgttgag acatcagagg cacttgttat cagagagcca 30780 ataactgtac cagagcgtcc tgaagacctg gaagtcaaag aagttactaa aaatactgta 30840 actttgactt ggaatcctcc taagtatgat ggtgggtcag aaattattaa ctatgtccta 30900 gaaagtcggc tcattgggac tgagaagttc cacaaagtta caaatgacaa cttgcttagc 30960 agaaaataca ctgttaaagg cttaaaagaa ggtgatacct atgagtaccg tgtcagtgct 31020 gtcaacattg ttggacaagg caaaccatca ttttgcacca aaccaattac ttgcaaggat 31080 gagctggcac ccccaacgct tcacctcgac ttcagagata agctcacgat tcgagttggt 31140 gaagcttttg ccctcactgg ccgttactca ggcaaaccaa agcctaaggt ttcctggttc 31200 aaagatgaag ctgatgtgct ggaagatgat cgcactcata taaagactac accagcaaca 31260 cttgctttag agaagatcaa ggccaaacgt tcagattccg gcaaatactg tgtggttgtg 31320 gagaacagta caggctctag gaaaggtttc tgtcaagtta atgttgttga ccatcctgga 31380 ccaccagtag gaccagttag ttttgatgag gtgaccaaag attacatggt tatctcttgg 31440 aagcctcctt tagatgatgg aggcagtaaa atcaccaatt atattattga gaagaaggaa 31500 gtgggtaaag acgtctggat gccagtgaca tctgcaagtg ctaaaacaac atgcaaagtt 31560 tctaaactac ttgaaggaaa agattatatt ttccggatac atgctgaaaa tctgtatgga 31620 ataagtgatc ctctggtgtc tgattcaatg aaagccaaag atcgtttcag ggttcctgat 31680 gcacctgatc agccaattgt tacagaagtt accaaagact ctgcattagt aacctggaat 31740 aagccacatg atggaggaaa acccatcaca aactacatcc tggaaaagag agaaactatg 31800 tctaaacgat gggctagagt taccaaagat cctattcatc catacactaa atttagggtt 31860 cctgatcttc tagaaggatg tcagtatgaa ttccgggttt ctgcagaaaa tgaaattggt 31920 attggagatc caagcccacc atccaaacca gtctttgcta aagatccaat tgctaaacca 31980 agtccacctg ttaatcctga agcaatagat acaacatgca attcagtcga tctaacttgg 32040 cagccaccac gtcatgatgg tgggagcaag attctgggtt atattgttga gtaccagaaa 32100 gttggagatg aagagtggag aagagccaat cacacccctg agtcatgtcc tgaaactaaa 32160 tataaagtca ccggtcttcg ggacggtcaa acctataagt ttagagtgtt agcagtcaat 32220 gcagctggtg aatcagatcc agctcatgtt ccggagccag tcctagtaaa agacaggctt 32280 gaaccccctg agttgattct tgatgccaac atggcaagag aacaacacat taaagttggt 32340 gatactctaa gacttagtgc catcatcaaa ggagtgccat tcccaaaagt aacttggaaa 32400 aaagaagaca gagatgctcc aactaaagca agaattgatg tgactccagt tggtagcaag 32460 cttgaaattc gtaatgctgc ccatgaagat ggtggaattt attctttaac agtggagaat 32520 ccagctggtt caaaaactgt ctcagtaaaa gtacttgtat tagataaacc tgggccacct 32580 agagatctgg aagtcagtga aattaggaaa gattcatgtt accttacttg gaaagaacca 32640 ctagatgatg gtggttctgt tattaccaat tatgtggttg agaggagaga tgttgccagc 32700 gcccagtggt cacctctctc agctacatca aagaaaaaga gtcacttcgc taagcatctg 32760 aatgaaggca accagtacct cttccgagta gctgcggaga accagtatgg acgtggtcct 32820 tttgttgaaa caccaaaacc aatcaaggct ttggatcctc tccatccccc agggccaccc 32880 aaggacctgc accatgtaga tgttgacaag actgaagtct ccctagtctg gaataagccg 32940 gatcgtgatg gtggttctcc aatcactgga tatttggtag aatatcaaga agaaggcacc 33000 caggactgga ttaaatttaa gactgtgaca aacttagagt gtgtggttac tggactacaa 33060 caaggaaaga cctatagatt ccgtgtaaaa gctgaaaaca ttgtgggtct tggtctccct 33120 gacacaacta tcccgataga atgtcaagaa aaactagtgc ctccatccgt ggagctagat 33180 gtgaaattaa ttgaaggtct tgtggtaaag gctggaacca cagtcagatt ccctgctatt 33240 ataagaggtg tgcctgttcc tactgcaaag tggacaaccg atgggagtga gattaaaacc 33300 gatgagcact acacagttga aacagacaac ttctcatcag tacttaccat taagaactgc 33360 ttaaggagag acactgggga atatcaaatc acagtttcca atgcagccgg tagcaaaaca 33420 gtagccgtac atcttactgt tcttgatgtt cctgggccac caacaggtcc tattaatatt 33480 ctggatgtta ctcctgaaca catgactatc tcatggcagc cacctaagga tgatggagga 33540 agccctgtga taaattatat tgttgagaaa caagatacaa ggaaagacac gtggggtgtt 33600 gtctcttccg gaagcagtaa gacaaagctg aaaatcccac atctgcagaa gggctgtgaa 33660 tatgttttcc gagttagagc agagaataag ataggtgttg gtcctcccct tgactccaca 33720 cctactgttg ctaagcataa atttagtcct ccgtctcctc ctggtaaacc agtggttact 33780 gacattactg aaaatgcagc aacagtgtct tggaccctgc caaaatctga tggtggcagt 33840 ccaataactg gctactatat ggaacgtcga gaagtaactg gcaaatgggt gagggtcaac 33900 aaaacaccta tcgctgacct gaagttcaga gtgactggac tctatgaagg aaatacatat 33960 gagtttagag tttttgctga aaatcttgca ggactaagca aaccatcccc aagttctgat 34020 ccaataaaag cttgccggcc catcaaacca cctggaccac ctattaatcc taaactgaaa 34080 gacaagagca gagaaacagc tgatttggtg tggacaaagc ctctcagtga tggtggtagc 34140 cccattctag gatatgtagt ggaatgtcag aaacctggca cggcacaatg gaacaggatt 34200 aataaagatg aactcattag gcaatgtgcc tttagggtac ctggactaat tgaaggaaat 34260 gagtacagat tccgtataaa ggcagctaat attgtaggag agggtgagcc aagagaacta 34320 gcagaatctg tgattgcaaa agatatcctt catcctccag aagtagaact tgatgttact 34380 tgtcgtgatg ttattaccgt gagagtaggc caaactatcc gcattctagc tcgagtcaaa 34440 ggcagacctg aaccagacat aacttggact aaggaaggca aagtattggt ccgagaaaag 34500 agggtggacc ttattcagga tctacctcgt gttgagttac aaattaaaga agctgttaga 34560 gctgatcatg gcaagtatat catctcagct aagaacagca gtggacatgc ccaaggttca 34620 gccatcgtta acgtccttga cagacctggg ccttgccaga atttgaaggt taccaatgta 34680 accaaagaga actgtacaat ttcttgggaa aacccactag ataatggtgg ctcagaaata 34740 acaaacttca tagtagaata tcgcaaacca aaccagaaag gctggtcaat tgttgcatca 34800 gatgtcacta aacgattaat caaggccaac cttttagcca acaatgaata ctatttccga 34860 gtttgtgcag agaataaagt aggtgttggg ccaaccatcg aaacaaaaac tcccattctg 34920 gctattaacc ctattgacag accaggtgag cctgaaaacc ttcacattgc agataaagga 34980 aagacatttg tctacctaaa gtggcggagg cctgactatg atggtggcag tccaaatctg 35040 tcatatcatg ttgagagaag gcttaagggc tccgatgact gggaaagagt gcataaagga 35100 agcattaaag aaactcacta catggttgac agatgtgttg aaaaccagat ttatgagttc 35160 agagtgcaaa caaagaatga aggtggggaa agtgactggg tgaagacaga ggaagttgtt 35220 gtgaaagaag acttacaaaa accagtactt gatctgaaat taagtggggt cctaactgtc 35280 aaagcagggg acaccattag gcttgaggca ggggttagag gcaaaccatt cccagaagtt 35340 gcatggacca aggacaaaga cgctacagac ttaacaagat caccaagggt caagattgat 35400 acccgtgctg attcatctaa attttctctt actaaagcaa agcgaagtga tgggggtaaa 35460 tatgtagtta cggcaactaa cacggctggc agttttgtgg cctatgccac tgtcaatgtt 35520 ttagataagc ctggtcctgt gagaaatctg aaaattgttg atgtgtccag tgataggtgt 35580 actgtttgct gggatccacc agaagatgat ggtggctgtg aaatccaaaa ttatattcta 35640 gaaaaatgtg agacaaagcg aatggtttgg tctacctatt ctgctactgt cttgacacct 35700 ggtactacag taacacgtct catagaagga aatgaatata ttttcagagt ccgtgcagaa 35760 aataaaatag gcacagggcc tccaacagaa agtaaaccag tcatagccaa aaccaagtat 35820 gataaacctg gtcgccctga tcccccagaa gtcactaaag taagcaaaga agagatgact 35880 gtggtttgga atccacctga atatgatggt ggaaagtcta taactggata ctttttggag 35940 aaaaaggaaa agcattcaac acgatgggtc cctgtcaaca agagtgcaat ccctgagaga 36000 cgtatgaaag tacagaatct cctcccagac catgaatatc agttccgtgt caaggcagaa 36060 aatgaaattg gaattggaga accaagcttg ccttcaagac cggtggtggc aaaagacccc 36120 atagagccac ctggtccacc aaccaatttc agagtggttg atacaaccaa acattccata 36180 actcttgggt ggggaaaacc agtctatgat ggtggtgcac cgatcattgg atatgttgtg 36240 gaaatgagac caaaaatagc agatgcgtct cctgatgaag gctggaaacg gtgtaatgct 36300 gcagcacagc ttgtacgcaa ggaattcact gttaccagct tggatgaaaa ccaggaatat 36360 gagttcaggg tgtgtgccca aaaccaagtt ggtattgggc gccctgcaga gctaaaggaa 36420 gctatcaaac ctaaagaaat actagaacct ccggagattg atttggatgc cagcatgagg 36480 aaactggtca tagtgagagc aggatgccct attcgtctct ttgctatagt gagaggacga 36540 ccagccccta aagtcacttg gcgaaaagtt ggcattgata atgtggtcag aaaaggacaa 36600 gttgatctgg ttgacactat ggccttcctt gtcatcccca attctacccg tgatgactca 36660 ggaaaatatt ccttaacact tgtgaaccca gcaggagaaa aggctgtatt cgtaaatgtc 36720 agagtattag acactcctgg gcctgtgtct gatttaaaag tttcagatgt cactaaaaca 36780 tcatgccatg tgtcctgggc ccctcctgaa aacgacggtg ggagccaagt gacacattat 36840 atcgtggaga aacgtgaggc agacagaaag acatggtcga ccgttacccc agaagttaag 36900 aaaacaagct tccatgtaac caatcttgtc cctgggaatg agtattactt cagagtaact 36960 gctgtcaacg aatatggccc tggcgtccca acagatgtcc caaaaccagt gcttgcatca 37020 gatcctctaa gtgagccgga tcccccaagg aaattagaag cgactgaaat gaccaagaac 37080 agtgccacct tagcctggtt acctccccta cgtgatggag gtgctaaaat cgatggctac 37140 atcattagtt acagagaaga agagcagcct gcagatcgct ggacagagta ctcagtggta 37200 aaagatctga gccttgttgt cactggccta aaggaaggaa agaaatacaa atttagagta 37260 gcggccagaa atgctgttgg agtcagtttg ccaagagaag ctgaaggagt gtatgaagcc 37320 aaagagcaac tgttgccacc aaagatcctt atgccagagc aaataactat caaagctggg 37380 aaaaaactcc gaattgaagc ccatgtgtat ggaaagcctc atcccacctg taaatggaaa 37440 aaaggagaag atgaagttgt cacatccagc cacctggcag tgcataaagc agacagctct 37500 tcaattctga tcataaaaga tgtgactagg aaagacagtg gttactacag cctcacagca 37560 gagaacagtt ctgggacaga cactcagaaa atcaaagttg tagtcatgga tgcccccggc 37620 ccccctcagc ctccatttga catttctgat atagacgctg atgcttgctc cctgtcatgg 37680 cacatccctc tggaggacgg aggcagtaac atcaccaatt atatagtgga gaagtgtgat 37740 gtaagccgag gtgactgggt cacggctcta gcttcagtca caaaaacttc ctgcagggtt 37800 ggaaagctga tcccaggcca agagtacatc ttccgggtcc gtgctgaaaa ccgatttggc 37860 atttcagagc ctctcacatc tccaaagatg gttgcgcagt tcccatttgg tgttcctagt 37920 gaaccaaaga atgcacgagt caccaaagtc aacaaggact gtatttttgt tgcttgggac 37980 agaccagata gtgatggagg gagccccatt attggttatc tgattgaacg caaggaaaga 38040 aacagtttgc tgtgggtgaa agccaatgat actcttgtcc ggtcaactga atatccttgt 38100 gctggccttg tagaaggtct tgagtattca ttcagaatct atgccctaaa caaagctgga 38160 tccagcccac ccagcaaacc cacagaatat gtaactgcaa gaatgccagt tgatcctcct 38220 gggaaacctg aggttattga tgtcaccaag agtactgtat ctctgatctg ggctcgtcca 38280 aagcatgatg gaggcagtaa aattattggc tatttcgtag aagcttgcaa acttcctggt 38340 gataaatggg tacggtgcaa tactgcacct caccagattc cccaggaaga gtacacagct 38400 actggcctag aagagaaagc tcagtatcaa tttagagcta ttgccaggac cgcggtaaac 38460 attagcccac cttctgaacc ttctgatcca gtgactatcc tcgcagaaaa tgtccctccc 38520 aggatagacc tgagtgtggc tatgaaatct ttgcttactg tgaaagctgg aactaatgtc 38580 tgcttggatg ctactgtttt tggtaaaccg atgccaacag tttcttggaa aaaagatggc 38640 acactgctaa aaccagcaga aggcataaag atggccatgc agcggaatct gtgcaccttg 38700 gagctattca gcgtgaaccg gaaggactca ggagactata ccattactgc tgaaaattca 38760 agtggttcta aatcagccac cattaagctt aaagtgttag ataaaccggg tcctccagca 38820 tctgttaaaa tcaacaaaat gtattcagat cgtgctatgc tttcttggga accgcctctt 38880 gaagatggag gctcagaaat caccaactat attgttgaca aacgtgaaac aagcaggccc 38940 aactgggctc aagtctctgc aactgtgcct atcaccagct gcagcgtgga gaaacttata 39000 gagggccatg agtatcagtt ccgtatttgt gctgaaaata aatatggagt aggcgatcca 39060 gtcttcactg aaccagcaat tgccaaaaac ccatatgacc caccaggacg ctgtgatcct 39120 cctgttatta gcaacataac caaagatcac atgacagtca gctggaagcc accagcagat 39180 gatgggggct cacccatcac tggctatttg cttgaaaagc gggaaaccca ggctgttaac 39240 tggactaagg tcaacagaaa acctattata gaaagaacat taaaagcaac aggtcttcaa 39300 gaaggtaccg aatatgagtt ccgtgttaca gctataaata aagctggacc aggcaaaccc 39360 agtgacgcat ccaaggccgc ttatgctcgg gaccctcagt atcctcctgc gccaccggct 39420 ttccctaaag tatatgatac aactcgcagc tctgtgagtc tatcttgggg caagccagcc 39480 tatgacggcg gcagccctat cattggttat ctcgttgaag taaaacgggc tgactccgat 39540 aactgggtga ggtgcaactt accacagaat ctacagaaaa cccgctttga ggttactggc 39600 ctgatggaag acacacaata tcaattccgt gtgtatgccg ttaataagat tggatacagt 39660 gaccccagtg atgtgccaga taaacactat cccaaggaca tcttaattcc acctgaggga 39720 gaacatgatg cggacttaag gaagacactc atattacgtg ctggagttac tatgagacta 39780 tatgtaccag taaaaggacg cccacctcca aagattactt ggtctaaacc aaatgtcaat 39840 ctaagagaca ggattggact ggacataaag tcaactgact ttgacacttt cttgcgctgt 39900 gaaaatgtga acaaatatga tgcaggaaaa tatatcttaa ccctggagaa cagctgtggt 39960 aaaaaggaat ataccattgt tgtgaaagtg cttgatactc ctgggccacc tatcaatgtg 40020 actgttaagg aaatatccaa agactctgct tatgttacct gggagcctcc cattattgat 40080 ggcggaagcc ccatcataaa ctatgtggta caaaaacgtg atgcagagag gaaatcctgg 40140 tctacagtga caactgagtg ctccaaaaca agcttcagag tacctaattt ggaggaggga 40200 aaatcctact tcttccgagt gtttgctgaa aatgagtatg gcattggtga tcccggtgaa 40260 actcgtgatg ctgtcaaagc ttcccaaact cctggaccag ttgtggacct gaaagtgagg 40320 tctgtatcta agtcatcctg tagcattggc tggaaaaagc ctcacagtga tggtggaagt 40380 cggattattg gatatgtagt tgatttcctg actgaagaaa ataagtggca acgagttatg 40440 aaatccttaa gcctacagta ctctgcaaaa gatttgactg aagggaagga atataccttc 40500 agagtgagtg ctgagaatga aaatggagaa ggaaccccaa gcgaaatcac tgttgtggca 40560 agggatgatg ttgtggctcc tgatcttgac ttaaagggtc tacctgattt gtgctacttg 40620 gctaaagaaa acagcaactt ccggcttaag atccccataa aaggcaagcc agctccatca 40680 gtctcctgga agaaagggga agatcctcta gcaactgaca ctagagtcag tgttgagtca 40740 tctgcggtta acacaactct tatagtgtac gattgccaaa aatctgatgc tggaaaatac 40800 acaatcacac ttaagaatgt tgctggcacc aaggaaggaa ctatctccat aaaggttgtt 40860 ggcaagcctg gcatccccac tggaccaatc aaatttgatg aagtcacagc agaagccatg 40920 accttaaagt gggctcctcc aaaggatgat ggaggttctg aaatcaccaa ctatatccta 40980 gagaagaggg attctgtgaa caacaagtgg gtgacgtgcg cctcagctgt ccagaaaacc 41040 acctttagag taaccagact tcatgagggc atggaatata ccttcagggt cagtgccgaa 41100 aataaatatg gtgtagggga aggcctgaaa tcggagccaa ttgttgcgag acatccattt 41160 gatgtgcctg atgctccccc acctcccaat attgtggatg tcagacacga ttcagtatct 41220 ctaacttgga ctgaccccaa gaaaactggt ggttctccaa ttacagggta tcatctcgag 41280 ttcaaggaaa gaaacagcct tttgtggaag agagctaaca agactccgat aaggatgaga 41340 gactttaaag tgacaggatt aactgaaggt cttgaatatg aattccgagt tatggcaatc 41400 aatttagcag gtgtgggcaa gccaagccta ccatcagagc ctgttgtggc actggaccca 41460 attgatcctc ctggaaaacc tgaggttatt aacataacaa ggaattcagt gactctcatt 41520 tggactgaac ctaaatatga cggtggtcat aagttaactg gatatatagt ggagaagcga 41580 gatctacctt cgaagtcttg gatgaaagcc aaccatgtta atgtcccaga atgtgccttt 41640 actgtaactg accttgttga gggtggaaaa tatgaattca gaattagagc aaagaataca 41700 gcaggtgcta tcagtgctcc atcagaaagt acagaaacca ttatttgcaa ggatgaatac 41760 gaggcaccaa caattgtcct tgatcccaca ataaaagatg ggctaacaat taaagcaggg 41820 gataccattg ttttgaatgc cattagcatt cttggcaaac cccttccaaa atcaagttgg 41880 tccaaggcag gaaaagacat tagaccatca gatatcactc agataacttc aaccccaaca 41940 tcttccatgc ttactatcaa gtatgccact agaaaagatg cgggtgaata taccatcact 42000 gctaccaatc cttttggcac gaaggtggaa catgtgaagg taacagtcct tgatgtacct 42060 ggtcccccag gtcctgttga aatcagtaat gtttctgctg aaaaagcaac acttacatgg 42120 acacctccct tggaagatgg cggctcacca attaagtcct atatacttga aaagagagaa 42180 accagccgac ttttgtggac agtggtttct gaagatattc agtcttgcag gcatgtggca 42240 accaaactta tccaaggaaa tgagtacatc ttccgggtct cagctgtaaa ccactatggc 42300 aaaggagaac ctgtacagtc tgaacctgtc aaaatggtag acagatttgg tccccctggc 42360 cctcctgaaa aaccagaggt atcaaatgtc actaagaaca ctgccactgt cagctggaaa 42420 aggccagtgg atgatggtgg cagcgaaatt acaggatatc atgtagaaag gagagaaaag 42480 aaaagcctgc gatgggtgag agcaataaaa acaccagttt ccgatctcag gtgcaaagta 42540 acaggactgc aagaaggaag cacctacgaa ttccgtgtca gtgcagaaaa cagagcagga 42600 attggtccac ccagtgaggc ttcagattct gttctgatga aagatgcagc atatcctcca 42660 ggaccacctt caaatccgca tgtcactgat actaccaaga aatctgcttc tttggcatgg 42720 ggcaagcctc attatgatgg tggacttgaa atcactggct atgtcgtgga gcatcaaaaa 42780 gtaggagacg aggcctggat aaaagatacc acaggaaccg ccctcagaat cactcagttc 42840 gttgttcctg atcttcagac taaagaaaaa tacaacttca gaatcagtgc catcaacgat 42900 gcaggtgttg gggagccagc ggtgattcca gatgttgaaa tcgtagaacg ggagatggct 42960 cctgattttg aactagatgc cgagcttcga agaacacttg ttgttagagc aggactcagt 43020 attaggatat ttgtgccaat taaaggtcgt cctgctcctg aagtgacatg gaccaaagat 43080 aacatcaacc tgaaaaaccg agccaacatt gaaaatacgg aatcatttac tcttctgatt 43140 atcccagaat gtaacagata tgataccggt aaatttgtca tgaccattga aaacccggct 43200 gggaagaaaa gtggctttgt gaacgtcaga gtcttggaca cgccaggccc agtcctcaac 43260 ctgcggccta cagacatcac aaaggacagt gtcaccctgc actgggacct ccctctgata 43320 gatggaggct cacgtataac aaactacatt gtagagaaac gtgaagcaac acggaaatct 43380 tattccacag ccaccactaa gtgccataaa tgcacatata aagttaccgg cttgtctgaa 43440 gggtgtgaat atttcttcag agtgatggca gagaatgaat atggaattgg tgagccaaca 43500 gaaactacag agcccgtaaa agcctctgaa gcaccatctc caccagacag ccttaacatc 43560 atggacataa ctaagagcac cgtcagcctg gcatggccta agcccaaaca cgatggtggc 43620 agcaagatca ctggctatgt gattgaagcc caaagaaaag gctctgacca gtggacccac 43680 atcacaaccg tgaaagggtt agaatgtgtt gtgaggaatc taactgaagg agaggaatat 43740 accttccaag tgatggcagt gaacagcgcg gggagaagtg cccctagaga aagcagaccc 43800 gtcattgtca aggagcagac aatgcttcca gagctggatc tccgtggcat ctatcagaaa 43860 ctggtcattg ccaaagctgg tgacaacatc aaagttgaaa ttccagtgct cggtcgaccg 43920 aagcccacag tgacatggaa aaaaggagac caaattctta aacagacaca gagagttaat 43980 tttgaaacca cagcgacttc aaccatttta aatatcaatg agtgtgtcag aagtgatagt 44040 gggccctatc cattaacagc aaggaacatt gtaggagagg ttggtgatgt catcaccatt 44100 caagtccatg atatcccagg gccacctact ggaccaatca aatttgatga agtttcatct 44160 gattttgtaa ccttctcttg ggacccacct gagaacgatg gtggtgtacc aataagcaac 44220 tatgtagtgg aaatgcggca gactgacagt actacctggg ttgagttagc aaccaccgtt 44280 atacgtacta cctataaagc cacccgcctt actactggat tagagtatca gttccgtgta 44340 aaagctcaga atagatatgg agttggacca ggcatcacat cagcatggat agttgccaac 44400 tatccattta aggttcctgg acctcctggt acccctcagg taactgcagt taccaaggat 44460 tcaatgacaa ttagctggca tgagccactt tctgatggtg gaagccccat tttaggatat 44520 catgttgaaa gaaaagaacg aaatggtatt ctctggcaga ctgtgagcaa agctttagta 44580 ccaggcaaca ttttcaaatc aagtggactt acagatggta ttgcttatga gttccgggtg 44640 attgcagaaa acatggcagg caaaagtaag ccaagcaagc catcagaacc tatgttggct 44700 ctggatccca ttgacccacc tggaaaacca gtacctctaa atattacaag acacacagta 44760 acacttaaat gggctaagcc tgaatatact gggggcttta aaattaccag ttatatcgtt 44820 gaaaagagag accttcctaa tggacggtgg ctgaaggcca acttcagcaa cattttggag 44880 aatgaattta cagtcagtgg cctaacagaa gatgctgcat atgaattccg tgtgatcgcc 44940 aaaaatgctg caggtgccat cagtccacca tctgagccat ctgatgctat cacttgcagg 45000 gatgatgttg aggcaccaaa gataaaggtg gatgttaaat ttaaggacac ggttatatta 45060 aaagcaggtg aagcattcag actggaagct gatgtttcag gccgcccacc tccaacaatg 45120 gaatggagca aagatggaaa agagctggaa ggcacagcaa agttagaaat aaaaattgca 45180 gatttctcta ctaatctggt aaacaaagat tcaacaagaa gggatagtgg tgcctatacc 45240 cttacagcga ctaatcctgg tggctttgct aaacacattt tcaatgtcaa agttcttgac 45300 agaccaggcc cacctgaagg acctttggct gtaactgaag tgacatcaga aaagtgtgta 45360 ctatcatggt tccctccact ggatgatgga ggtgccaaaa ttgatcatta catagtacag 45420 aaacgtgaaa ccagcagatt ggcatggaca aatgtagcct cagaagtcca agtaacaaag 45480 ctaaaggtca ctaaactctt gaaaggcaat gaatacatat tccgtgtcat ggctgtaaat 45540 aaatatggag tgggagagcc actggaatca gagcctgtgc ttgcagtgaa tccttatgga 45600 ccccctgatc cgcccaaaaa ccctgaagtg acaactatta ctaaagattc gatggttgtc 45660 tgctggggac atcctgattc tgatggtgga agtgaaatca tcaattatat tgtggaacgg 45720 cgtgataaag ctggccaacg ctggattaaa tgcaacaaaa aaactcttac tgatttaaga 45780 tataaagtgt ctggactgac agaaggacat gaatatgagt tcaggattat ggctgaaaat 45840 gctgctggaa ttagtgcacc aagtcctacc agtccatttt acaaggcttg tgacactgtg 45900 tttaaacctg gaccaccagg taacccacgt gttctggata caagcagatc atccatttca 45960 atcgcttgga ataaacctat ctatgatggt ggttcagaaa tcactgggta tatggttgag 46020 attgccctgc cagaggaaga tgaatggcag attgtcactc caccagcagg actcaaggca 46080 acttcgtata ctatcactgg cctcacagag aatcaggaat ataagatccg catctatgcc 46140 atgaattccg aaggacttgg ggaacctgcc cttgttcctg gaactccaaa ggctgaagac 46200 agaatgctgc ctccagaaat tgaactggat gctgacctgc gcaaagttgt tactataagg 46260 gcctgctgca ccctgagact ttttgttccc atcaaaggaa ggcctgaccc tgaggtgaag 46320 tgggcccggg accatggaga atctttagat aaagctagca tcgaatccgc aagctcttac 46380 accctgctta ttgttggaaa tgtaaacaga tttgacagtg gcaaatatat actaactgta 46440 gaaaatagtt caggcagcaa gtctgcattt gtcaatgtta gagttctcga tacaccaggc 46500 cccccacagg atctgaaggt aaaagaggtc actaagacat ctgtcacact cacatgggac 46560 ccacctctcc ttgatggagg ttcaaaaatc aagaactata ttgttgaaaa gcgggaatca 46620 acaagaaaag catattcaac tgttgcaaca aactgccaca agacttcctg gaaggtagac 46680 cagcttcaag aaggctgtag ctactatttc agggttctcg cagaaaatga atatggcatt 46740 gggctgcctg ctgaaaccgc agaatctgtg aaagcatcag aacgacctct tcctccagga 46800 aaaataactt tgatggatgt cacaagaaat agtgtgtcac tctcttggga gaaaccagag 46860 catgatggag gcagccgaat tctaggctac attgtggaga tgcagaccaa aggcagtgac 46920 aaatgggcca cgtgtgccac agtcaaggtc actgaagcca ctatcactgg attaattcag 46980 ggtgaagaat actctttccg tgtttcagct cagaatgaaa agggcatcag tgatcctaga 47040 caactgagtg tgccagtgat cgccaaagat cttgtcattc caccagcctt caaactcctg 47100 ttcaatactt tcactgtact ggcaggtgaa gacctaaaag ttgatgttcc attcattggc 47160 cgccctaccc cagctgtaac ctggcataaa gataatgtac cactgaagca gacaactaga 47220 gtaaatgcag agagcacaga aaataattca ctactgacaa taaaggacgc ctgccgagaa 47280 gatgttggcc attatgtggt taaactgact aactcagctg gtgaagctat tgaaaccctt 47340 aatgttatcg ttcttgacaa accagggcct ccaactggac cagttaaaat ggatgaagtg 47400 acagctgata gtattactct ttcctggggc ccacccaagt atgatggcgg aagttctatc 47460 aataattaca ttgttgagaa acgggacact tccacaacca cctggcaaat tgtatcagct 47520 acagttgcaa ggacaacaat aaaggcttgc agactgaaga ctggatgtga atatcagttt 47580 agaattgcag ctgaaaacag atatgggaag agtacctacc tcaattcaga gcctactgta 47640 gcccaatatc cattcaaagt tcctggtcct cctggcactc cagttgtcac actgtcctcc 47700 agggacagca tggaagtaca atggaatgag ccaatcagtg atggaggaag tagagtcatt 47760 ggctatcatc tagaacgcaa ggaaagaaat agcatcctct gggttaagtt gaataaaaca 47820 cctattcctc aaaccaagtt taagacaact ggccttgaag aaggtgttga atatgaattt 47880 agagtctctg cagagaacat cgtgggcatt ggcaagccga gtaaagtatc agaatgttat 47940 gtggctcgtg acccatgtga tccaccagga cggccagagg caatcattgt cacaaggaat 48000 tctgtgactc ttcagtggaa gaaacccacc tatgacggtg gaagcaagat cactggttat 48060 attgttgaga agaaagaatt acctgagggc cgttggatga aagccagttt tacaaatatt 48120 attgacactc attttgaagt aactggccta gttgaagatc acagatatga gttccgggtt 48180 atagcccgaa atgccgcagg agtgtttagt gagccttcag aaagcacagg agcaataaca 48240 gctagagatg aggtagatcc accacgaata agtatggatc caaaatacaa agacacaatc 48300 gtggttcatg ctggtgaatc attcaaggtt gatgcagata tttatggcaa accaatacca 48360 accattcagt ggataaaagg tgatcaggag ctttcaaaca cagctcgatt agaaataaag 48420 agcaccgact ttgccaccag tctcagtgta aaagatgcag tacgtgtcga cagtggaaat 48480 tacatactga aggccaaaaa tgttgcagga gaaagatcag ttactgtgaa tgtcaaggtt 48540 cttgacagac cagggccacc tgaaggacct gttgttatct caggagttac agcagaaaaa 48600 tgcacactag cttggaaacc cccacttcag gatggtggga gtgacatcat aaattatatt 48660 gtggaaagga gagaaaccag ccgcttagtt tggactgtgg ttgatgccaa tgtgcagact 48720 ctcagctgca aggttactaa gcttcttgaa ggcaatgaat atactttccg tataatggca 48780 gtaaacaaat atggtgttgg tgaacctctt gaatctgagc cagtagttgc caagaatcca 48840 tttgtagtac cagatgcacc aaaagctcca gaagtcacaa cagtgaccaa ggactcaatg 48900 attgttgtat gggaaagacc agcatctgat ggtggtagtg aaattcttgg atatgttctt 48960 gagaaacggg ataaagaagg cattagatgg acaagatgcc ataagcgtct gattggagag 49020 ttgcgcctga gagtaactgg actcatagaa aatcacgatt atgagttcag agtttctgct 49080 gagaatgctg ctggacttag tgaaccaagc cctccttctg cttaccaaaa ggcttgtgat 49140 cctatttata aaccaggacc cccaaacaac cccaaagtca tagacataac cagatcttca 49200 gtattccttt cttggagcaa accaatatat gatggtggct gtgaaattca aggatacatt 49260 gttgaaaaat gtgatgtgaa tgttggtgaa tggacaatgt gcactccacc aacaggaatt 49320 aataaaacaa acatagaagt agagaagctg ttggaaaagc atgaatacaa cttccgtatc 49380 tgtgctatta ataaagctgg agttggagaa catgctgacg tccctggacc tattatagtt 49440 gaagaaaaat tagaagcacc agacattgat cttgacctag aactaaggaa aatcataaat 49500 ataagggcag gtggctcctt aaggttattt gttcctataa aaggtcgtcc tacaccagaa 49560 gttaaatggg gaaaggtgga tggtgaaatc cgagatgcag ctataattga tgtcactagc 49620 agtttcacct ctcttgttct tgacaatgtc aaccgatatg atagtggaaa atatacgctt 49680 acattagaaa acagcagtgg aacaaagtct gcctttgtta ctgtgagagt tctggacacg 49740 ccaagtccac ctgttaacct gaaagtcaca gaaatcacca aagactcagt atcaattaca 49800 tgggaacctc ctttgttgga tgggggatcc aaaataaaaa attacattgt tgagaaacgt 49860 gaagccacaa gaaaatcata tgctgctgtt gtaactaact gccataagaa ttcttggaaa 49920 atcgatcagc tccaagaagg ttgcagttat tactttagag tcacagctga gaatgagtat 49980 ggtattggcc ttcctgccca gactgctgat ccaattaagg ttgcagaagt gccacaacct 50040 cctggaaaaa taactgtgga tgatgtcacc agaaacagtg tctctctgag ttggacaaaa 50100 cctgaacatg atggtggcag taaaatcatt cagtatattg tggaaatgca agctaaacac 50160 agtgagaaat ggtcagagtg tgctcgagta aagtctcttc aggcagtaat taccaaccta 50220 actcaagggg aagaatatct ttttagagtt gttgctgtaa atgaaaaggg gagaagtgat 50280 cctcggtccc ttgcagttcc aatagttgcc aaagatctgg taattgagcc agatgtaaaa 50340 cctgcattca gtagttacag tgtacaggtt ggccaagatt tgaaaataga agtgccaatt 50400 tctggacgtc ctaagccaac cattacctgg actaaagatg gtctcccact gaagcagacc 50460 acaagaatca atgttaccga ttcactggat ctcaccacac tcagtattaa agaaactcat 50520 aaggatgatg gtggacaata tggaatcaca gttgccaatg ttgttggtca gaagacagca 50580 tccatcgaaa ttgtaactct agataaacct gatcctccaa aaggacctgt taaatttgat 50640 gacgtcagtg ctgaaagtat tacattatct tggaaccctc cattatatac agggggctgc 50700 caaatcacca actacattgt tcagaaaaga gatacaacca ccacagtatg ggatgttgtt 50760 tctgctactg ttgctagaac tacactcaaa gtgaccaaac tgaaaactgg tacagaatac 50820 caatttagaa tatttgccga aaacagatat ggacaaagct ttgccttaga gtctgatcca 50880 attgtagctc aatatcccta caaagaacca ggccctccag gtacaccatt tgccacagcc 50940 atttccaaag actccatggt catacagtgg catgaaccag tcaacaatgg tggaagcccc 51000 gtcataggtt accacctgga gagaaaagaa agaaacagta ttttgtggac aaaggtcaac 51060 aaaactatta ttcatgacac ccaattcaaa gcacagaatc ttgaagaagg cattgaatat 51120 gaattcagag tgtatgctga aaatattgtt ggtgtaggca aagcaagcaa gaattctgaa 51180 tgctatgtag ccagagatcc ctgtgaccca ccaggaaccc cagaaccaat aatggttaaa 51240 agaaatgaaa tcactttaca gtggaccaaa cctgtgtatg atggtggaag tatgattaca 51300 ggctacattg tagagaaacg tgatttgcct gatggtcgtt ggatgaaagc tagctttaca 51360 aatgtcattg aaactcaatt tactgtgtca ggtcttactg aagatcaaag atatgaattc 51420 agagtcattg caaagaatgc agctggtgca ataagtaaac cctctgacag tactggacca 51480 ataactgcca aggatgaggt tgaactccca agaatttcaa tggatccaaa attcagagac 51540 acaattgtgg taaatgctgg agaaacattc agacttgagg ctgatgtcca tggaaagccc 51600 ctacctacca ttgagtggtt aagaggagat aaggaaattg aagaatctgc tagatgtgaa 51660 ataaagaaca cagatttcaa ggctttactt attgtaaaag atgcaattag aattgatggt 51720 gggcagtata ttttaagagc ttccaatgtt gcaggttcta agtcattccc agtaaatgta 51780 aaagtattag atagaccagg acctccagaa gggccagtcc aggttactgg agtcacttct 51840 gaaaaatgct ctttaacatg gtctccacca cttcaagatg gtggcagtga catttctcac 51900 tatgttgttg aaaagcgaga aaccagtcga cttgcctgga ctgttgttgc ttcagaagtt 51960 gtgaccaatt ctctgaaagt taccaaactc ttagaaggta atgaatatgt tttccgtata 52020 atggctgtca acaaatatgg tgttggagag cctttggaat ctgcaccagt actaatgaaa 52080 aatccatttg tgcttcctgg accaccaaaa agcttggaag tcacaaatat tgccaaagac 52140 tccatgaccg tctgttggaa ccgtccagat agtgatggtg gaagtgagat tattggttac 52200 attgtagaga aaagagacag aagtggcatt cgatggataa aatgtaataa acgccgcatt 52260 acagatttgc gtctaagagt gacaggatta acagaagatc atgagtatga attcagggtc 52320 tctgcagaaa atgctgctgg agttggggaa ccaagtccag ctacagttta ttataaagcc 52380 tgtgatcctg tgttcaaacc tggcccacct accaatgcac acattgtaga caccactaaa 52440 aattcaatca cacttgcctg gggtaaaccc atctatgatg gcggcagtga gatcttggga 52500 tatgtagtag aaatctgtaa agcagatgaa gaagaatggc aaatagttac tccacagact 52560 ggcctgagag tcactcgatt tgaaatttca aaactcactg aacaccaaga gtataaaata 52620 cgagtctgtg ccctcaacaa agttggttta ggtgaggcta catcagttcc tggtactgtg 52680 aaaccagaag ataaacttga agcacctgaa cttgaccttg actccgaatt aagaaaagga 52740 attgttgtaa gagctggtgg atctgccaga attcacattc cattcaaagg tcgtccaatg 52800 cctgagatca cttggtctcg agaggaaggt gaattcacag ataaggtcca aattgaaaag 52860 ggagtaaact atacccaact atcaatagat aactgtgata gaaatgatgc tggaaaatac 52920 attcttaagt tggaaaacag cagtggatca aagtctgctt ttgtaactgt gaaagttctt 52980 gacactccag gaccaccaca gaatttggca gtcaaagaag tgagaaaaga ttctgccttc 53040 ctggtatggg agccacccat cattgatgga ggggcaaagg tcaagaacta tgtgattgac 53100 aaacgtgagt caaccagaaa agcgtatgct aatgtgagta gtaaatgcag caaaacaagt 53160 tttaaagtgg aaaaccttac agaaggagcc atttattact tcagagtcat ggctgaaaat 53220 gaatttggag ttggtgttcc agtggaaact gttgatgccg tgaaagctgc tgaacctcct 53280 tccccaccag gaaaggttac actcactgat gtgtcccaga ccagtgcatc acttatgtgg 53340 gagaaacctg aacatgatgg cggtagcaga gtcctggggt acgttgttga aatgcagccc 53400 aaaggaactg aaaaatggag cattgtggct gaatccaaag tctgtaatgc agttgttact 53460 ggtttgagtt ctggacaaga atatcagttc cgtgtcaagg cttataatga gaaaggaaaa 53520 agcgatccaa gagtgttggg tgttcctgtc atagccaagg acttgactat acagcctagt 53580 ttaaagttac catttaacac atatagtatc caagctggag aagatcttaa aatagaaatt 53640 ccagttatag gccgaccaag acctaacatt tcttgggtca aagatggtga gcctcttaaa 53700 cagacaacaa gagtaaacgt tgaagaaaca gctacctcaa ctgttttgca cattaaagaa 53760 ggtaacaaag atgactttgg aaaatacacc gtaacggcaa caaatagtgc aggcacagca 53820 acagaaaatc tcagtgttat cgttttagaa aagcctggac ctccagttgg cccagttcgg 53880 tttgatgaag ttagtgcaga ctttgtagtc atatcttggg aacctccagc ctatactggt 53940 ggctgccaaa taagcaacta cattgtagag aagcgagata caaccaccac cacttggcac 54000 atggtatcag caacagttgc aagaacaaca attaaaataa ccaaactgaa aacaggcacg 54060 gagtaccagt ttagaatttt tgctgaaaac aggtatggaa aaagtgcccc actggattct 54120 aaggcagtta ttgtacaata tccatttaaa gaacctggac cacctggaac tccttttgtg 54180 acatcaatct caaaagatca gatgcttgtg caatggcatg agccagtgaa tgatggaggc 54240 accaaaatta ttggctacca tcttgaacag aaagaaaaga acagtatttt atgggtcaag 54300 ttaaataaga cccccattca ggacaccaaa ttcaaaacaa ctgggcttga tgagggcctt 54360 gagtatgagt tcaaagtttc tgctgaaaat attgttggca ttggcaagcc tagcaaagtg 54420 tcagaatgct ttgttgctcg tgatccatgt gacccacctg gtcgccctga agccattgtt 54480 attacaagaa acaatgtcac actgaaatgg aagaaacctg cctatgatgg tggtagcaaa 54540 ataacaggtt atattgtaga aaagaaagat ctacctgatg gccgctggat gaaagccagc 54600 tttaccaacg tattagaaac tgaatttaca gtgagtggac ttgtagaaga ccaaagatat 54660 gaatttagag taattgcaag aaatgcagct ggaaacttta gtgaaccatc tgatagtagt 54720 ggtgccatta ctgcaagaga tgaaattgat gcaccaaatg cctctctgga tccaaaatat 54780 aaagatgtca tcgttgttca tgcaggagag acttttgttc ttgaagccga catccgtggc 54840 aaacctatac ctgatgttgt ttggtcaaaa gatggaaaag aacttgaaga aacagctgct 54900 agaatggaaa ttaaatctac tattcagaaa acaactcttg ttgtcaaaga ctgtatacgg 54960 actgatggag gacaatatat tctgaaactc agcaatgttg gtggtacaaa gtctataccc 55020 atcactgtaa aggtacttga caggccaggg tctcctgaag ggcctctgaa agttactgga 55080 gttactgcgg aaaaatgtta cctggcatgg aacccacctt tgcaagatgg tggtgctaat 55140 atttcacatt acatcattga aaagagggag acaagccgac tctcttggac ccaggtttca 55200 actgaggtac aggcccttaa ctacaaagtt actaaacttc ttcctggtaa tgagtacatt 55260 ttccgtgtca tggctgtgaa taaatatgga attggagagc ccttggaatc tgggcctgtt 55320 acggcctgta atccttataa gccaccaggt cctccctcaa cacctgaagt ctcagcaatc 55380 accaaagatt ctatggtagt aacatgggca cgcccagtag acgacggagg taccgaaatt 55440 gagggctaca ttcttgaaaa acgagataag gaaggcgtta gatggaccaa gtgcaacaag 55500 aaaacattaa cggatctgcg gctcagggta actggtctta ccgaaggcca ttcctatgaa 55560 ttcagagttg ctgctgaaaa tgcagctggt gtgggagaac ctagtgagcc atctgttttc 55620 taccgtgcgt gtgatgcctt gtatccacca ggtcccccaa gcaatccaaa agtgacggac 55680 acttccagat cttctgtctc cctggcatgg agtaagccaa tttatgatgg tggcgcacct 55740 gttaaaggct atgttgtaga ggtcaaagaa gctgctgcgg atgaatggac aacctgcact 55800 ccaccaacag gattacaagg aaagcagttc acagtgacca agcttaaaga aaacactgaa 55860 tataacttcc gtatttgtgc catcaattct gaaggtgtag gtgaacctgc aactctacct 55920 ggctcagtgg ttgctcagga gaggatagag ccaccagaaa tagaactcga tgctgatctc 55980 agaaaggtgg tcgttctgcg tgcaagtgct actttacgct tatttgtcac tatcaaaggt 56040 cgaccagaac ccgaagttaa atgggaaaag gcagaaggca ttctcactga cagggctcag 56100 atagaggtga ccagctcatt tacaatgttg gtgattgata atgttaccag atttgacagt 56160 ggtcggtata atctgacatt agaaaataat agtggctcca aaacagcttt tgttaacgtc 56220 agagttcttg actcaccaag tgcccctgtg aatttgacca taagagaagt gaagaaagac 56280 tcagtgacgt tgtcctggga accaccactt attgatggtg gagctaagat tacaaactac 56340 attgtcgaaa aacgagaaac tacaagaaaa gcctatgcta ccattacaaa taattgcact 56400 aaaactactt tcagaattga aaatctacaa gaaggatgtt cttactactt ccgagtcttg 56460 gcttccaatg aatatgggat tggtttgcca gctgaaacaa cagaacccgt taaagtgtct 56520 gaaccacccc tcccacctgg aagagtaact cttgttgatg tgacccgtaa tacagctaca 56580 attaagtggg agaaaccaga aagtgatggt ggcagcaaaa ttactggtta tgtggttgaa 56640 atgcagacta aagggagtga aaagtggagc acctgcacac aagttaagac tctagaagca 56700 actatatctg gcttaactgc aggagaagag tatgtcttca gggtagctgc agttaacgaa 56760 aagggaagaa gtgatccaag acaacttgga gtgccagtaa ttgcaaggga tattgaaata 56820 aagccttcag ttgagcttcc tttccatact ttcaatgtaa aggctagaga acaacttaag 56880 attgatgtgc cattcaaagg aagacctcaa gctactgtga actggagaaa agatggtcag 56940 actcttaaag agacaactag agtcaatgtt tcttcttcaa agactgtaac atcactatct 57000 attaaggaag cttcaaagga agatgttgga acttatgaat tatgtgtttc aaacagtgct 57060 ggatccataa cagttcctat tactataatt gtccttgaca gaccaggacc tccaggtcct 57120 atacgtattg atgaggttag ttgtgacagc ataaccattt cttggaatcc tccagaatat 57180 gatggtggct gccaaattag caattacatt gttgaaaaga aagaaaccac ctctacaaca 57240 tggcacatag tttcacaagc agttgcaaga acatccatta aaatagttcg cctgacaaca 57300 ggaagtgagt atcagttccg tgtttgtgca gaaaaccgct atggaaagag ctcctacagt 57360 gaatcttcag ctgttgttgc agagtatcca ttcagtcccc caggtcctcc tggtactcct 57420 aaagttgtgc atgccacaaa atctaccatg cttgtaacct ggcaagtgcc agttaatgat 57480 ggaggaagtc gagtaattgg ctatcatctt gagtataaag aaagaagcag cattctttgg 57540 tcaaaagcaa ataaaatcct cattgctgat actcaagtga aagtctccgg ccttgatgaa 57600 ggactgatgt atgagtatcg tgtatatgct gaaaatattg ctggaattgg taaatgcagt 57660 aaatcttgtg aaccagtccc tgcaagagat ccttgtgacc ctcctggaca acctgaagtc 57720 acaaatatca caagaaaatc agtgtcactt aaatggtcta aaccacatta tgatggtgga 57780 gctaagatca caggatacat tgttgaacgc agagaactac cagatggccg gtggctaaag 57840 tgcaattata ctaatataca agaaacatac tttgaagtaa ctgaacttac tgaagatcag 57900 cgttatgaat tccgggtttt tgcaaggaat gctgctgact cagttagtga gccatctgaa 57960 tccactgggc ctattatagt taaagatgat gttgagcctc caagagttat gatggatgtc 58020 aagttccgag acgttattgt tgtcaaagct ggagaggtcc ttaagataaa tgcagacatt 58080 gcagggcgac ctctgccagt aatttcctgg gccaaggatg gtatagaaat tgaagaaaga 58140 gcaagaacag aaatcatctc aacagacaat catactttgt taacagttaa agactgtata 58200 agacgagaca ctgggcaata tgtactaaca ctgaagaatg ttgccggcac tcggtctgtg 58260 gccgttaatt gcaaagtact tgataagcct ggtccaccag caggaccact tgaaataaat 58320 ggcctcactg ctgagaaatg ctctctttcc tggggacgtc cccaagaaga tggtggtgca 58380 gatatcgact attaccatcg taaaaaacgt gaaacaagcc accttgcatg gacaatatgt 58440 gaaggagagt tacagatgac atcctgtaaa gtaaccaagt tactcaaagg caatgaatat 58500 atatttagag taactggtgt taataaatat ggtgttggtg agcccctaga gagtgtagct 58560 ataaaggcac tagatccatt tacagttcca agtccaccca cgtctttgga aattacttct 58620 gtgaccaaag aatctatgac actttgctgg tcaagacccg agagtgatgg aggtagtgaa 58680 atatctggat atataattga aaggcgagag aaaaatagcc taagatgggt gcgtgtaaac 58740 aaaaaaccag tttatgatct aagagtgaaa tcaacaggac ttcgggaagg atgtgaatat 58800 gaatatcgtg tttatgcaga aaatgctgct ggcctaagtc ttccaagtga aacctctccc 58860 ttaattaggg cagaagatcc agtgttccta ccatctcctc catccaaacc caaaattgtg 58920 gactcaggca agacaactat aactattgcc tgggttaagc cgctgtttga tggtggggcc 58980 ccgataactg gatatactgt agaatacaaa aaatctgatg acactgactg gaaaacttcc 59040 attcagagct tacgagggac agaatataca ataagcggac taacaacagg agctgaatat 59100 gttttcagag taaaatctgt caataaggtt ggtgctagtg accccagtga tagctctgac 59160 cctcagatag caaaggaaag agaagaagaa cctttatttg atattgacag tgaaatgagg 59220 aagaccttga ttgtcaaggc tggtgcctca tttaccatga ctgtgccttt ccgaggaaga 59280 ccagtaccca atgtcttgtg gagtaagcca gacactgacc tccgtactag agcttatgtt 59340 gataccacag actctcgtac atcactgacc attgaaaatg ccaacagaaa tgactctgga 59400 aagtacacat taacaattca gaatgttttg agtgctgctt cactgacctt agttgtcaaa 59460 gttttagata ccccaggtcc tccaaccaac attactgtgc aagatgtaac caaagagtct 59520 gcagtgttat cctgggatgt tcctgaaaac gatggtggag caccagtgaa gaattaccac 59580 atagaaaaac gtgaggccag caagaaagca tgggtctctg tgaccaacaa ctgtaaccgc 59640 ctctcctaca aagttaccaa tttacaagaa ggagctatct attacttcag agtctctgga 59700 gaaaatgagt ttggtgttgg tataccagct gaaacaaagg aaggagtaaa aataacagaa 59760 aaaccaagcc cacctgaaaa acttggagta acaagtatat ccaaagacag tgtttccctg 59820 acctggctga agcctgaaca tgatggcgga agcagaattg tacactatgt cgttgaagca 59880 ctagaaaaag gacagaaaaa ctgggttaaa tgtgcagtgg caaagtcaac ccatcacgtt 59940 gtttccggtc tgagagagaa ttctgaatac tttttccgag tgtttgctga aaatcaagct 60000 ggcctgagtg acccgagaga gcttctgctt cctgttctta ttaaggagca actagaacca 60060 cctgaaattg atatgaagaa tttcccaagt cacactgtat atgttagagc tggttcaaac 60120 cttaaagttg acattccaat ctctggaaaa ccacttccca aagtgacctt atcaagagat 60180 ggtgtccccc ttaaggcaac catgagattt aataccgaaa ttactgctga gaacctgacc 60240 atcaatctca aagaaagtgt tacagctgac gctgggagat atgaaatcac tgctgccaac 60300 tccagtggta caaccaaagc tttcattaac attgttgtgc tagacaggcc tggtcctcca 60360 actggccctg ttgttattag tgatataact gaagaaagtg tgactctcaa atgggagcca 60420 cctaagtatg acggtggaag tcaagttacc aactacattc tactcaaaag agaaacaagt 60480 actgcagtgt ggactgaagt gtctgcaaca gttgcaagaa ccatgatgaa agtcatgaaa 60540 ctgaccacag gagaagaata ccaattccgc atcaaggcag aaaaccgctt tggcatcagt 60600 gatcatatag attcagcttg tgtgactgtc aaactaccat acacaacacc tggaccacca 60660 tctacaccat gggtcactaa tgttactcga gaaagcatca ctgtgggctg gcatgaacca 60720 gtgtcaaatg gaggcagtgc agtcgtaggc tatcacctgg aaatgaaaga cagaaacagt 60780 attttatggc aaaaagccaa caaactggtc atccgcacaa ctcacttcaa agtcacaaca 60840 atcagtgctg gacttattta tgaattcagg gtgtatgcag aaaatgctgc tggagttgga 60900 aaacctagcc atccttctga accagtcttg gcaattgatg cttgtgaacc cccaagaaat 60960 gttcgtatca ctgatatttc aaagaactct gtcagccttt catggcaaca accagctttc 61020 gatggaggta gcaagattac aggctacatt gttgagagac gtgaccttcc agatggcaga 61080 tggaccaagg ccagcttcac caatgttact gaaactcaat tcaccatctc tggcttgact 61140 cagaattccc agtatgaatt ccgtgtcttt gctaggaatg ctgttggttc cattagcaat 61200 ccatctgagg ttgtagggcc cattacttgc atcgattctt atggtggtcc tgtaattgat 61260 ttgcctctag aatatacaga agttgtcaaa tacagagcag gtacatctgt gaagctcaga 61320 gctggcattt ctggcaaacc tgcgcctact attgagtggt ataaagatga taaagaatta 61380 caaaccaatg cactggtgtg tgttgaaaat accacggacc tcgcatctat actcatcaaa 61440 gatgccgatc gccttaatag tggatgctat gaattaaaac taaggaatgc catggcgtca 61500 gcctcagcca ccatcagagt acagatcctt gacaaaccag gcccacctgg tggaccaatt 61560 gaatttaaga ctgtaactgc tgagaagatc acccttctct ggcggcctcc agctgatgat 61620 ggtggtgcaa aaatcactca ctacattgtg gaaaagcgtg agacaagccg cgttgtgtgg 61680 tctatggtgt ctgaacattt ggaagagtgc atcattacaa ccaccaaaat tatcaaagga 61740 aatgaataca tcttccgggt ccgagccgtg aacaaatatg gaattggcga gccactggaa 61800 tctgattccg ttgtagccaa gaacgcattt gttacacctg ggccaccagg cataccagaa 61860 gtgacaaaga ttaccaagaa ttcgatgact gttgtatgga gcaggccaat tgcagatggc 61920 ggtagtgata taagtggcta tttccttgaa aaacgagaca agaagagcct aggatggttt 61980 aaagtactaa aagagactat ccgtgacacc agacaaaaag taacaggact cacagaaaac 62040 agtgactatc aatacagagt ttgtgctgta aacgctgctg gacagggtcc attttctgaa 62100 ccatctgaat tctacaaagc tgctgatcct attgatcctc caggtccacc tgctaagata 62160 agaatcgcag attcaaccaa gtcatccatc acccttggct ggagtaagcc tgtctatgat 62220 gggggcagtg ctgttactgg gtatgttgtc gagataagac aaggagagga agaggaatgg 62280 actactgtct ctaccaaagg agaggtcaga actacagaat atgtggtatc caacctgaaa 62340 cctggagtca attactactt ccgggtatct gctgtaaact gtgctggaca aggagaacct 62400 atagaaatga atgaacctgt acaagctaaa gatatacttg aggcaccaga gattgacctg 62460 gatgtggctc tcagaacttc tgttattgcc aaagctggtg aagatgtaca agtgttgatt 62520 ccctttaaag gcagacctcc acctactgtc acatggagaa aagatgagaa gaatcttggc 62580 agtgatgcca gatacagcat tgaaaacact gattcatcct cattactcac cattcctcaa 62640 gttactcgca atgatacagg aaaatatatt ctcacaatag aaaatggagt tggtgaacct 62700 aagtcttcaa ctgtgagtgt taaagtgctt gacacaccag ctgcctgcca gaaactacag 62760 gttaaacatg tttctcgagg cacagtcact ttgctctggg atcctcctct cattgatgga 62820 ggatctccaa taattaatta tgtcattgaa aagagagatg ccaccaagag aacatggtct 62880 gtcgtgtcac acaaatgttc tagcacatcc ttcaagctaa tagatttgtc ggagaagact 62940 ccattcttct tcagagttct tgcagaaaat gaaattggaa ttggggaacc ctgtgaaact 63000 acagagccag tgaaggctgc tgaagtacca gctcctatac gtgatctctc aatgaaagac 63060 tcaacaaaga catctgtcat cctcagctgg accaaacctg actttgatgg tggtagcgtc 63120 atcacagaat atgttgtaga aaggaaaggt aaaggtgaac agacgtggtc ccacgctggc 63180 ataagtaaga catgtgaaat tgaggttagc caacttaagg agcagtcagt cctggagttc 63240 agagtgtttg ccaaaaatga gaaaggactg agtgatcctg tcactattgg gccaattaca 63300 gtgaaagaac ttattattac acctgaagtt gacctgtcag atatccctgg ggcacaagtc 63360 actgtgagaa ttgggcacaa tgtgcacctt gaattacctt ataagggaaa acccaaacca 63420 tccatcagtt ggctgaaaga tggcttgcca ctgaaagaaa gtgaatttgt tcgcttcagt 63480 aaaactgaaa acaaaattac tttgagtatt aagaatgcca agaaggagca tggaggaaaa 63540 tacactgtta ttcttgataa tgcagtgtgt agaattgcag tccccattac agtcatcacc 63600 cttggcccac catcaaagcc caaaggaccc attcgatttg atgaaatcaa ggctgatagt 63660 gtcatcctgt catgggatgt acctgaagat aatggaggag gagaaattac ttgttacagc 63720 atcgagaagc gggaaacttc acaaactaac tggaagatgg tgtgttcaag tgttgccaga 63780 acgactttca aagttcctaa tctagtcaaa gatgctgaat accagtttag agtgagagca 63840 gaaaacagat acggagtcag ccaaccactt gtctcaagca ttattgtggc aaaacaccag 63900 ttcaggattc ctggtccccc aggaaagcca gttatataca atgtgacttc tgatggcatg 63960 tcactaactt gggatgctcc agtttatgat ggtggttcag aagttactgg attccatgtt 64020 gaaaagaaag aaagaaatag catcctctgg caaaaagtta atacatcacc aatctctgga 64080 agagaatata gagccactgg actggtagaa ggtctggatt accaattccg tgtatatgct 64140 gaaaattctg ctggcctaag ctcacctagt gacccaagca aatttacctt agctgtttct 64200 ccagtagacc cacctggcac tcctgactac attgatgtca cccgggaaac catcacactt 64260 aaatggaacc caccattgcg tgatggaggc agtaagattg tgggctatag cattgagaaa 64320 cggcaaggaa atgaacgctg ggtgagatgc aactttactg acgtcagtga atgtcagtac 64380 acagttacag gactcagtcc tggggatcgc tatgagttca gaataattgc aagaaatgct 64440 gttggaacta taagcccgcc ctcacagtct tctggcatta ttatgacaag agatgaaaat 64500 gttccaccaa tagtagagtt tggccctgaa tactttgatg gtctcattat taagtccgga 64560 gagagcctta gaattaaagc tttggtacaa ggaagaccag tgcctcgagt aacttggttc 64620 aaagatggag tggaaatcga aaagaggatg aatatggaaa taaccaacgt acttggatcc 64680 accagcctat ttgttagaga tgctactcgg gaccatcgtg gtgtatacac agtggaagcc 64740 aaaaatgcat ctggttctgc aaaagcagaa attaaagtga aagtacaaga tacaccagga 64800 aaagtagttg ggccaataag attcaccaat attactgggg agaagatgac tctgtggtgg 64860 gatgccccac tcaatgacgg ttgtgctccc ataacccact acatcattga aaaacgggaa 64920 accagcagac ttgcctgggc actaattgag gataaatgtg aagcccaaag ttacactgcc 64980 attaaactaa taaacggcaa tgaataccaa ttccgtgttt ctgcagttaa caagtttggt 65040 gttggcaggc cacttgattc tgatccagtg gttgctcaaa tacaatatac tgttcctgat 65100 gcccctggca ttccagaacc tagcaacata acaggcaaca gcattaccct gacatgggca 65160 aggccagaat cagatggtgg cagtgaaatt caacagtata tccttgaaag aagagaaaag 65220 aaaagcacaa gatgggtaaa agtgatcagc aaacgaccaa tctctgaaac aagattcaaa 65280 gtcactggtc tgacagaagg caatgagtat gaattccatg tcatggctga aaatgctgca 65340 ggagttggac ctgcaagtgg catctcaaga ctcattaaat gtagagagcc cgtcaaccca 65400 ccaggtcctc ccacagtggt caaagtaaca gacacatcaa agacaactgt gagcttagaa 65460 tggtccaaac cagtgtttga tggtggcatg gaaataattg ggtatattat tgaaatgtgt 65520 aagaccgact taggagactg gcacaaggtg aatgcagagg catgtgtgaa aacaagatat 65580 acagtcactg atctacaagc aggtgaagaa tacaaattcc gagttagtgc tatcaatggt 65640 gctggaaaag gcgacagctg tgaagtgact ggcacaatta aagcagttga ccggttaaca 65700 gctcctgagt tagacataga tgcaaacttc aaacagactc atgttgttag agctggggcc 65760 agtattcgcc tcttcattgc ctaccaaggt agacctactc ctacagctgt gtggagcaaa 65820 ccagactcta accttagcct tcgggctgat atccatacaa cagattcctt cagcaccctc 65880 actgtggaaa actgcaacag aaatgatgca gggaaatata cccttactgt ggaaaacaac 65940 agtggtagta agtcaatcac attcaccgtg aaagtgctag acactccagg cccacctggc 66000 ccaattacct tcaaagatgt gacccgggga tctgctacat tgatgtggga tgcccctctt 66060 cttgacggtg gtgcccgaat ccatcattat gtggtagaga aacgagaggc aagtcgccgt 66120 agttggcagg ttatcagtga aaaatgcact cgtcagatct tcaaggtcaa tgacctggcc 66180 gaaggtgttc cgtactattt ccgtgtttct gcagtaaatg agtatggtgt tggtgagccc 66240 tatgaaatgc cagaaccaat tgtagccacg gaacagcctg ctccacctag gagacttgat 66300 gttgttgata ctagcaaatc ctccgcagtc ttagcttggc ttaaacctga ccacgatgga 66360 ggcagccgga tcactggcta cctgcttgaa atgagacaaa agggatctga cctctgggtt 66420 gaagctggtc acaccaaaca gctaactttc acagtagagc gtcttgttga gaaaactgaa 66480 tatgaattcc gtgtgaaggc caagaatgat gctggctata gtgaacccag agaagccttc 66540 tcttctgtca tcattaagga gcctcaaatc gagcccactg ctgacctcac tggaattacc 66600 aatcagctta taacttgcaa agcaggaagc ccatttacca ttgacgtacc aatcagtggt 66660 cgtcctgccc ccaaagtaac atggaaactg gaagaaatga gacttaaaga gacagatcga 66720 gtgagcatta caacaacaaa agacagaacc acactgactg taaaggacag catgagaggt 66780 gactctggaa gatacttctt gaccctggaa aatacagctg gtgttaaaac atttagcgtc 66840 acagttgtgg tcattggaag gccaggtcca gtaaccggcc ccattgaggt ctcatctgtc 66900 tcagctgaat cgtgtgtcct gtcatgggga gaacctaaag atggaggagg cactgaaatt 66960 actaattaca tagttgaaaa gcgtgaatcg ggtacaacag cttggcagct tgtcaattcc 67020 agtgtcaagc gcactcaaat taaagtcact catctcacaa aatacatgga atattctttc 67080 cgtgtcagtt cagagaacag atttggtgtc agcaaacctc tagaatcagc accaataatt 67140 gctgaacatc catttgtccc accaagcgct cctaccagac ctgaggtcta ccatgtgtct 67200 gccaatgcca tgtctattcg ttgggaagaa ccctaccacg atggtggcag taaaatcatt 67260 ggctactggg ttgagaagaa agaacgtaat acaattcttt gggtgaaaga aaacaaagtg 67320 ccatgcttag agtgcaacta caaagtaact ggtttagtag aaggactgga atatcagttc 67380 agaacttatg cactcaatgc tgcaggtgtt agcaaggcca gcgaagcttc aagacctata 67440 atggctcaaa atccagttga tgcaccaggc agaccagagg tgacagatgt cacaagatca 67500 acagtatcac tgatttggtc tgccccagcg tatgatggag gcagcaaggt tgtgggctac 67560 atcatagagc gtaagccagt cagtgaggta ggagatggtc gctggctgaa gtgcaactac 67620 accattgtat ctgacaattt cttcaccgtg actgctctca gtgaaggaga cacttatgag 67680 ttccgtgtgt tagccaagaa tgcagcaggc gtaattagca aagggtctga atctacaggc 67740 cctgtcactt gccgagatga atacgctcca cccaaagccg aactggatgc ccgattacac 67800 ggtgatctgg ttaccatcag agcaggttct gatcttgttc tggatgctgc agttggtggc 67860 aaacctgaac ccaaaattat ctggaccaaa ggagacaagg agctagatct ctgtgaaaaa 67920 gtctctttgc agtatactgg caaacgagca actgctgtga tcaagttctg tgacagaagt 67980 gacagtggaa aatacacttt aacagtgaaa aatgccagcg ggaccaaggc cgtgtctgtc 68040 atggtcaaag tgcttgattc ccctggccca tgtggaaagc tcaccgtcag cagagtaaca 68100 caggagaagt gcactttagc ctggagcctt ccgcaggaag acggaggagc agaaatcact 68160 cactacatcg tggaaagacg cgagactagc aggctcaact gggtgattgt tgaaggcgaa 68220 tgcccaaccc tatcctatgt cgttaccagg ctcatcaaga acaatgagta catattccga 68280 gtgagggcag taaacaaata tggccctggt gtgcctgttg aatcagagcc aattgtagcc 68340 agaaactcat tcactattcc atcaccaccc ggcatacctg aagaagttgg gactggcaaa 68400 gagcatatca tcattcagtg gacaaaacct gaatctgatg gtggcaatga aatcagcaac 68460 tacctagtag acaaacgtga gaaggagagc ctgcgctgga cacgtgtcaa caaagactat 68520 gtggtgtatg ataccaggct gaaggtgacc agcctgatgg agggttgtga ttaccagttc 68580 cgggtgaccg cagtgaatgc agctggtaac agtgagccca gcgaacgttc caacttcatc 68640 tcatgcagag aaccatcata tacccctgga ccaccttctg ctccaagagt tgtggatacc 68700 accaaacaca gcattagttt ggcatggacc aaacccatgt acgatggtgg tactgacatt 68760 gtaggatatg ttctggaaat gcaagagaag gacactgatc agtggtaccg agtgcatacc 68820 aatgccacaa taagaaatac tgaattcact gtgccagacc ttaaaatggg ccagaaatat 68880 tccttcagag ttgctgccgt gaacgtgaag ggtatgagcg aatacagcga atcaattgct 68940 gaaattgagc ccgtggaaag aatagaaata ccagatcttg agcttgcaga tgatctaaag 69000 aagactgtga ccatcagggc tggggcctcc ttgcgcttga tggtgtctgt atctggaaga 69060 ccacctcctg tcataacgtg gagcaagcag ggcattgacc ttgcaagccg ggcaattatt 69120 gacaccactg agagctactc attgctaata gtggacaaag ttaatcggta cgatgctgga 69180 aaatacacaa ttgaagctga aaaccaatct ggcaagaaat cagcaacagt ccttgttaaa 69240 gtctatgata ctcctggtcc ctgtccttca gtgaaagtta aggaagtatc aagagattct 69300 gtgactataa cttgggaaat tcccacgatt gatggtggag ctccaatcaa caattacatc 69360 gttgagaagc gtgaagctgc tatgagagca ttcaaaacag taactaccaa atgcagcaag 69420 acactttaca gaatttctgg acttgtagaa ggaaccatgc actatttcag agtgctgcca 69480 gaaaatattt atggcattgg agaaccttgt gaaacatctg atgcagtact ggtctcagaa 69540 gtgcctttgg tgcctgcaaa gctagaagtg gtcgatgtca ccaaatccac tgttaccctt 69600 gcctgggaaa aaccactcta cgatggtggt agccgactca ctggatatgt tctcgaggcc 69660 tgcaaagctg gcacagagag atggatgaag gttgtcacct taaaacccac agtcctagag 69720 cacactgtta cttccttaaa tgaaggtgaa caatacttat ttagaataag ggcacaaaat 69780 gagaaaggtg tgtcagaacc aagagagact gtcacagccg tgactgtaca agacctcaga 69840 gtgttgccaa caatcgatct ttctacaatg cctcagaaga ccatccatgt cccagctggc 69900 agaccagtag agctggtgat acctattgct ggccgtccac ctcctgctgc ttcctggttc 69960 tttgctggtt ctaaactgag agaatcagag cgtgtcacag ttgaaactca cactaaagta 70020 gctaaattaa ccatccgtga aaccactatc agagatactg gagaatacac acttgaattg 70080 aagaatgtta ccggaactac ttcagaaacc attaaagtta tcattcttga caagcctggt 70140 ccaccaacag gacctattaa gattgatgaa attgatgcta catcaattac catttcctgg 70200 gaaccacctg aattggacgg tggtgctcca ctgagtggtt atgtggtaga acaacgtgac 70260 gctcatcgtc caggatggct gcccgtttct gaatcagtga ctaggtccac gtttaagttt 70320 accagactca ccgaaggaaa tgagtatgtg ttccgtgtgg ctgcaacaaa ccgcttcggg 70380 attggctctt acttgcagtc tgaggtcata gagtgtcgca gcagcatccg tattcctgga 70440 cccccagaaa cattacagat atttgatgtt tcccgtgatg gcatgacact tacttggtac 70500 ccaccagagg atgacggtgg ctcccaagtg actggatata ttgtggagcg caaagaagtg 70560 agagcagatc gatgggtccg tgtaaataaa gtacctgtga caatgacacg gtaccgctcc 70620 actggcctta ctgaaggctt agaatatgaa caccgtgtca cagccattaa tgcaagaggg 70680 tctgggaaac caagtcgtcc ttccaaaccc atcgttgcca tggatccaat tgctcctcca 70740 ggaaagccac aaaacccaag agttactgat acaacaagga catcagtctc cctggcctgg 70800 agtgttccag aagatgaagg aggatctaaa gtcacaggct acttgattga aatgcaaaaa 70860 gtagatcaac atgaatggac caagtgtaac accactccaa ccaagattcg agagtatact 70920 ctaacacacc tacctcaggg tgcagaatac aggttccgcg tcctagcttg taatgctggt 70980 ggacctggtg agcctgctga ggtaccagga acagtcaaag tcactgaaat gcttgaatat 71040 cctgattatg aacttgatga aagataccaa gaaggtatct ttgtaaggca aggtggcgtc 71100 atcagactta ccataccaat caaaggaaaa ccattcccaa tatgtaaatg gaccaaggaa 71160 ggccaggata ttagtaagcg tgccatgatt gcaacatctg aaacacacac tgagcttgtg 71220 atcaaagaag cagacagggg tgattctggc acttatgacc tggttctgga aaataaatgt 71280 ggcaagaagg ctgtctacat caaggtcagg gtgataggaa gtcccaacag tccagaaggg 71340 ccactggaat atgatgacat ccaagtccgc tctgtgaggg tcagctggag acctcctgct 71400 gatgatggtg gtgctgacat cttaggctac atcctcgaga gacgagaagt gcctaaagcc 71460 gcctggtata ccattgattc cagagtccga ggtacatctc tggtggtaaa aggcctcaaa 71520 gagaatgtag aataccattt ccgtgtttca gcagaaaacc agtttggcat aagcaaaccc 71580 ttgaaatctg aggaaccagt cacaccaaaa acaccattga atcctccaga acctccaagc 71640 aatcctccag aagtactcga tgtaaccaag agttctgtta gcttgtcctg gtcccggccc 71700 aaagatgatg gtggttctag agtcacaggc tactacatcg aacgcaaaga gacatccact 71760 gacaagtggg tcagacacaa caagactcag atcaccacca caatgtacac tgtcacaggg 71820 cttgttcccg atgctgagta tcagttccgc atcatcgcac agaatgatgt tggcctgagt 71880 gagaccagcc ctgcttctga accagttgtt tgcaaagatc catttgataa accaagccaa 71940 ccaggagaac ttgagattct ttcaatatcc aaagatagtg tcactctaca gtgggagaaa 72000 cctgaatgtg atggtggtaa agaaattctt ggatactggg ttgaatatag acagtctgga 72060 gacagtgcct ggaagaagag caataaggaa cgtattaagg acaagcaatt cacaatagga 72120 ggtttgctgg aagctactga gtatgaattc agggtttttg ctgagaatga gactgggctg 72180 agcagacctc gcagaactgc tatgtctata aagactaaac tcacatctgg agaggcccca 72240 ggaatacgca aagaaatgaa ggatgttacc acaaaattgg gtgaagctgc tcaactctca 72300 tgccagattg ttggaaggcc tcttcctgac attaaatggt acagatttgg taaagagctc 72360 atacaaagcc ggaaatacaa aatgtcttca gatggacgca cacacactct tacagtaatg 72420 acagaggaac aggaagatga aggtgtttat acctgcatag ccaccaatga ggttggagaa 72480 gtagaaacca gtagtaagct tctcctgcaa gcaacaccgc agttccatcc tggttaccca 72540 ctgaaagaga aatattatgg agctgtgggt tccacacttc ggcttcatgt tatgtacatt 72600 ggtcgtccag tacctgccat gacttggttc catggtcaga aacttttgca aaactcagaa 72660 aacattacta ttgaaaacac tgagcactat actcatcttg tcatgaagaa tgtccaacgt 72720 aagactcatg ctgggaaata caaagtccag ctcagcaatg tttttggaac agttgatgcc 72780 atccttgatg tggaaataca agataaacca gacaaaccta caggaccaat tgtgatcgaa 72840 gctctattga agaactccgc agtgatcagc tggaaaccac ccgcagatga cggaggctcc 72900 tggatcacca actatgtggt ggaaaaatgt gaggccaagg agggggctga atggcaattg 72960 gtgtcttcag ccatctcagt gacaacctgt agaattgtga acctcacaga aaatgctggc 73020 tattacttcc gggtttcagc tcagaacact ttcggcatca gtgaccctct agaagtgtcc 73080 tcagttgtga tcattaagag tccatttgaa aagccaggtg ctcctggcaa accaactatt 73140 actgctgtca caaaagattc ttgtgttgtg gcctggaagc cacctgccag tgatggaggt 73200 gcaaagatta gaaattacta ccttgagaag cgtgagaaga agcagaataa atggatttct 73260 gtgacaacag aagaaattcg agaaactgtc ttttcagtga aaaaccttat tgaaggtctt 73320 gaatacgagt ttcgtgtgaa atgtgaaaat ctaggtgggg aaagtgaatg gagtgaaata 73380 tcagaaccca tcactcccaa atctgatgtc ccaattcagg caccacactt taaagaggaa 73440 ctgagaaatc taaatgtcag atatcagagc aatgctacct tggtctgcaa agtgactggt 73500 catccaaaac ctatcgtcaa atggtacaga caaggcaaag aaatcattgc agatggatta 73560 aaatatagga ttcaagaatt taagggtggc taccaccagc tcatcattgc aagtgtcaca 73620 gatgatgatg ccacagttta ccaagtcaga gctaccaacc aagggggatc tgtgtctggc 73680 actgcctcct tggaagtgga agttccagct aagatacact tacctaaaac tcttgaaggc 73740 atgggagcag ttcatgctct ccgaggtgaa gtggtcagca tcaagattcc tttcagtggc 73800 aaaccagatc ctgtgatcac ctggcagaaa ggacaagatc tcattgacaa taatggccac 73860 taccaagtta ttgtcacaag atccttcaca tcacttgttt tccccaatgg ggtagagaga 73920 aaagatgctg gtttctatgt ggtctgtgct aaaaacagat ttggaattga tcagaagaca 73980 gttgaactgg atgtggctga tgttcctgac ccacccagag gagtcaaagt tagtgatgcc 74040 tcacgagatt ctgtcaactt aacatggact gagccagcct ctgatggtgg cagcaaaatc 74100 accaactaca ttgttgaaaa atgtgcaact actgcagaaa gatggctccg tgtaggacag 74160 gcccgagaaa cacgttatac cgtgatcaac ttatttggaa aaacaagtta ccagttccgg 74220 gtaatagctg aaaataaatt tggtctgagc aagccttcag agccttcaga accaaccata 74280 accaaagaag ataagaccag agctatgaac tatgatgaag aggtagatga aaccagggaa 74340 gtctccatga ctaaagcatc tcactcttca accaaggaac tctatgagaa atatatgatt 74400 gctgaagatc ttgggcgtgg tgagtttgga attgtccatc gttgtgttga aacatcctca 74460 aagaagacat acatggccaa atttgttaaa gtcaaaggga ctgatcaggt tttggtaaag 74520 aaggaaattt ccattctgaa tattgctagg catagaaaca tcttacacct ccatgaatca 74580 tttgaaagca tggaagaatt agttatgatc tttgagttta tatcaggact tgacatattt 74640 gagcgcatta acacaagtgc ttttgaactt aatgaaagag aaattgtaag ttatgttcac 74700 caggtctgtg aagcacttca gtttttacac agtcataata ttggacactt tgacattaga 74760 ccagaaaata tcatttacca aaccagaaga agctctacca ttaaaatcat agaatttggt 74820 caagcccgtc agctgaaacc aggggacaac ttcaggcttc tattcactgc cccagaatac 74880 tatgcacctg aagtccacca gcatgatgtt gtcagcacag ccacagacat gtggtcactt 74940 ggaacactgg tatatgtgct attgagtggt atcaacccat tcctggctga aactaaccaa 75000 cagatcattg agaatatcat gaatgctgaa tatactttcg atgaggaagc attcaaagag 75060 attagcattg aagccatgga ttttgttgac cggttgttag tgaaagagag gaaatctcgc 75120 atgacagcat cggaggctct ccagcaccca tggttgaagc agaagataga aagagtcagt 75180 actaaagtta tcagaacatt aaaacaccgg cgttattacc acaccctgat caagaaagac 75240 ctcaacatgg ttgtgtcagc agcccggatc tcctgtggtg gtgcaattcg atctcagaag 75300 ggagtgagtg ttgctaaagt taaagtggca tccattgaaa ttggcccagt ttctgggcag 75360 ataatgcatg cagttggtga agaaggagga catgtcaaat atgtatgcaa aattgaaaat 75420 tatgatcagt ctacccaagt gacttggtac tttggtgtcc gacagctgga gaacagtgag 75480 aaatacgaaa tcacctacga agatggagtg gccatcctct atgtcaaaga cattaccaaa 75540 ttagatgatg gtacctacag atgcaaagta gtcaatgact atggtgaaga cagttcttat 75600 gcagagctat ttgttaaagg tgtgagagaa gtctatgact attactgccg tagaaccatg 75660 aagaaaatta agcgcagaac agacacaatg agactcctgg aaaggccacc agaatttacc 75720 ctgcctctct ataataagac agcttatgta ggtgaaaatg tccggtttgg agtaactata 75780 actgtccacc cagagcctca tgtaacatgg tataaatcag gtcagaaaat caaaccaggt 75840 gacaatgaca agaagtacac atttgagtca gacaagggtc tttaccaatt aacaatcaac 75900 agtgtcacta cagatgatga cgctgaatat actgttgtgg caaggaacaa atatggtgaa 75960 gacagctgta aagcaaagct gacagtaacc ctacacccac ctccaacaga tagtacctta 76020 agacccatgt tcaaaaggtt actggcaaat gcagaatgcc aagaaggcca aagtgtctgc 76080 tttgagatca gagtgtctgg catcccccca ccaacattaa aatgggagaa agatggtcag 76140 ccactgtccc tcgggcctaa cattgaaatt atccatgaag gcttggatta ttatgctctg 76200 cacatcaggg acactttgcc tgaagacacg ggttattata gagtcacagc cactaacaca 76260 gctgggtcca ccagctgcca ggctcaccta caagtggaac gcctgaggta caagaaacag 76320 gaattcaaga gtaaggagga gcatgagcga cacgtacaaa aacaaattga caaaaccctc 76380 agaatggctg aaattctttc tggaactgaa agtgtaccac tgacacaggt agctaaagag 76440 gctctgagag aagctgctgt cctttataaa ccggctgtaa gcaccaagac tgtaaaaggg 76500 gaattcagac ttgagataga agaaaagaag gaggagagaa aactccggat gccttatgat 76560 gtaccagagc cacgcaagta taagcagact accatagaag aagaccaacg catcaagcag 76620 ttcgtgccca tgtctgacat gaagtggtat aaaaagatac gtgatcagta tgaaatgcct 76680 gggaaacttg acagagttgt acagaaacga cccaagcgca tccgcctttc aagatgggaa 76740 cagttctatg tgatgcctct tccacgcatt acagatcaat acagacctaa atggcgtatt 76800 cctaaactgt cccaagatga tcttgagata gtgagaccag cccgccggcg tacaccttct 76860 cctgattatg acttttacta ccgacctaga agacgttctc ttggggacat ctctgatgaa 76920 gaattactcc tccccattga tgactactta gcaatgaaaa gaacagagga agagaggctg 76980 cgtcttgaag aagagcttga gttaggtttt tcagcttcac ccccaagtcg aagccctcca 77040 cactttgagc tttctagcct acgttactct tcaccacaag ctcatgtcaa ggtggaggaa 77100 acaagaaaaa acttcaggta ttcaacctat cacatcccaa cgaaggctga agctagtaca 77160 agttatgcag aactgaggga acggcatgcc caggctgcgt acagacagcc aaagcaacgg 77220 caaagaatca tggctgagag ggaggatgaa gagttgcttc gcccagttac gaccacccag 77280 catctctcag aatacaaaag cgaacttgac ttcatgtcaa aggaggaaaa gtctagaaag 77340 aaatcaaggc gacaaagaga agtgacagaa ataacagaaa ttgaggaaga atacgaaatc 77400 tcaaaacatg ctcaaagaga atcatcctca tctgcgtcta gactactgag acgacggcgc 77460 tccctgtctc caacttatat tgagttaatg aggccagtgt ctgagctgat ccggtcacgt 77520 ccacaaccgg ctgaggaata cgaagatgac acagaaagaa ggtcacctac tccagagaga 77580 actcgcccac gatcccccag ccctgtgtct agtgagagat cactctcgag atttgagagg 77640 tctgcaagat ttgatatctt ttccaggtat gagtccatga aagctgcttt aaaaactcag 77700 aagacatcag aaaggaagta tgaagttttg agtcagcagc ctttcacact ggaccatgcc 77760 cctcgaatca cactgagaat gcgctcgcac agggtaccat gtggccaaaa tacacgtttt 77820 attttaaatg ttcagtctaa gccaactgcc gaggttaaat ggtaccacaa tggtgtggaa 77880 ctccaagaaa gcagtaagat tcattacacc aacacgagtg gagtcctcac cctggaaatt 77940 ctggactgtc atactgatga cagtggaacc taccgtgctg tgtgcaccaa ctacaagggc 78000 gaagcttctg actatgcaac gttggacgtg acaggagggg attataccac ctatgcttcc 78060 caacgcagag atgaagaggt ccccagatct gttttccctg agctgacaag aacagaggcg 78120 tatgctgttc catcatttaa gaaaacatct gagatggaag cttcgtcttc tgtcagggaa 78180 gtgaaatcac agatgacgga gacaagggaa agtctctcct catatgaaca ctctgcatct 78240 gcagaaatga aaagtgctgc attagaagaa aagtcactgg aagaaaaatc cacaaccaga 78300 aagatcaaga cgactttggc agcaagaatt ctaacaaagc cacggtccat gaccgtctac 78360 gagggcgagt ctgcaaggtt ttcttgtgac accgatggtg agccggtacc aactgtgacc 78420 tggctgcgta aaggacaagt gctaagtact tctgcccgcc accaagtgac caccacaaag 78480 tacaaatcaa cctttgagat ctcttcagtc caggcttccg atgagggcaa ttacagcgtg 78540 gtggtagaaa acagtgaagg gaaacaagaa gcagagttca ctctgactat tcaaaaggcc 78600 agggtaactg aaaaggctgt gacatcacca ccaagagtca aatccccaga gcctcgggtg 78660 aaatccccag aagcagttaa gtctccaaaa cgagtgaaat ctccagaacc ttctcacccg 78720 aaagccgtat cacccacaga gacaaaacca acaccaagag agaaagttca gcacctccca 78780 gtctctgccc caccaaagat tactcagttc ctgaaagcag aagcttctaa agagattgca 78840 aaactgacct gtgtggttga aagcagtgta ttaagggcaa aagaggtcac ctggtataaa 78900 gatggcaaga aactgaagga aaatgggcat ttccagtttc attattcagc agatggtacc 78960 tatgagctca aaatcaataa cctcactgaa tctgatcaag gagaatatgt ttgtgagatt 79020 tctggtgaag gtggaacgtc taaaaccaac ttacaattta tggggcaagc ctttaagagt 79080 atccatgaga aggtatcaaa aatatcagaa actaagaaat cagatcagaa aaccactgag 79140 tcaacagtaa ccagaaaaac tgaaccaaaa gctcctgaac caatttcctc aaaaccagta 79200 attgttactg ggttgcagga tacaactgtt tcttcagaca gtgttgctaa atttgcagtt 79260 aaggctactg gagaaccccg gccaactgcc atctggacaa aagatggaaa ggccattaca 79320 caaggaggta aatataaact ctctgaagac aagggagggt tcttcttaga aattcataag 79380 actgatactt ctgacagtgg actttatact tgtacagtaa aaaattcagc tggatctgtg 79440 tcctctagct gcaaattaac aataaaagct ataaaagata ctgaggcaca gaaagtctct 79500 acacaaaaga cttctgaaat tacacctcag aagaaagctg ttgtccaaga ggaaatttcc 79560 caaaaagccc taaggtctga agaaattaag atgtcagagg caaaatctca agaaaagtta 79620 gccctcaaag aggaagcttc aaaggttctg atttctgaag aagtcaagaa atcagcagca 79680 acctccctgg aaaaatccat tgtccatgag gaaatcacta aaacatcaca ggcatcagaa 79740 gaagtcagaa ctcatgctga gattaaagca ttttctactc agatgagcat aaacgaaggt 79800 caaagactgg ttttaaaagc caacattgct ggtgccactg atgtgaaatg ggtactgaat 79860 ggcgtagagc ttaccaactc tgaggagtac cgatatggtg tctcaggcag cgatcagacc 79920 ctaaccatca agcaagccag tcacagagat gaaggaatcc tcacctgcat aagcaaaacc 79980 aaggaaggaa tcgtcaagtg tcagtatgat ttgacactga gcaaagaact ctcagatgct 80040 ccagccttca tctcacagcc tagatctcaa aatattaatg aaggacaaaa tgttctcttt 80100 acttgtgaaa tcagtggcga gccatcccct gaaatcgaat ggtttaaaaa caacctgcca 80160 atttctattt cttcaaatgt cagcataagc cgctccagaa atgtatactc ccttgaaatc 80220 cgaaatgcat cagtcagcga cagtggaaag tacacaatta aggccaaaaa tttccgtggc 80280 cagtgttcag ctacagcttc cttaatggtc cttcctctag ttgaagaacc ttccagagag 80340 gtagtattga gaacaagtgg tgacacaagc ttgcaaggaa gcttctcgtc tcagtcagtc 80400 caaatgtctg cctccaagca ggaggcctcc ttcagcagtt tcagcagcag cagtgctagc 80460 agcatgactg agatgaaatt tgcaagcatg tctgcccaaa gcatgtcctc catgcaagag 80520 tcctttgtag aaatgagttc cagcagcttt atgggaatat ctaatatgac acaactggaa 80580 agctcaacta gtaaaatgct taaagcaggc ataagaggaa ttccgcctaa aattgaagct 80640 cttccatctg atatcagcat tgatgaaggc aaagttctaa cagtagcctg tgctttcacg 80700 ggtgagccta ccccagaagt aacatggtcc tgtggtggaa gaaaaatcca cagtcaagaa 80760 caggggaggt tccacattga aaacacagat gacctgacaa ccctgatcat catggacgta 80820 cagaaacaag atggtggact ttataccctg agtttaggga atgaatttgg atctgactct 80880 gccactgtga atatacatat tcgatccatt taagagggcc tgtgccctta tactctacac 80940 tcattcttaa cttttcgcaa acgtttcaca cggactaatc tttctgaact gtaaatattt 81000 aaagaaaaaa agtagttttg tatcaaccta aatgagtcaa agttcaaaaa tattcatttc 81060 aatcttttca taattgttga cctaagaata taatacattt gctagtgaca tgtacatact 81120 gtatatagcc ggattaacgg ttataaagtt ttgtaccatt tattttatga cattttacaa 81180 tgtaagtttt gaaactaact gttggtagga gaaagtttct tatggaacga ataccctgct 81240 caacatttaa tcaatctttg tgcctcaaca tactgttgat gtctaagtat gcctcagtgg 81300 gttgagaaaa tccccattga agatgtcctg tccacctaaa agagaatgat gctgtgcata 81360 tcacttgata tgtgcaccaa tacctactga atcagaaatg taaggcattg gtgatgtttg 81420 catttaccct cctgtaagca acactttaac gtcttacatt ttctctgatg atgtcacaca 81480 aaattatcat gacaaatatt accagagcaa agtgtaacgg ccaacacttt gttcgctcat 81540 tttacgctgt ctctgacata aggagtgcct gaatagcttg gaaaagtaac atctcctggc 81600 catcccttca tttaaccaag ctattcaagt attcctatgc cagagcagtg ccaactcttg 81660 gaggtcccag agtgcagcca atgcctttgt gtggtagttc taaattttaa ttgcacctga 81720 aaaacctggg cacctaagca atgagccaca gcaaaaagta aagaacaaca acaaaataaa 81780 gctgttgtta aattttaaac aatattacta attgcccaaa atgtcaattt gatgtagttc 81840 ttttcatgca agtataaatt caattgttag ttataattgt tggacctcct tgagatagta 81900 acaacaaaat aaagcaagct atctgcacct caaaaaaaaa 81940 2 26926 PRT Homo sapiens 2 Met Thr Thr Gln Ala Pro Thr Phe Thr Gln Pro Leu Gln Ser Val Val 1 5 10 15 Val Leu Glu Gly Ser Thr Ala Thr Phe Glu Ala His Ile Ser Gly Phe 20 25 30 Pro Val Pro Glu Val Ser Trp Phe Arg Asp Gly Gln Val Ile Ser Thr 35 40 45 Ser Thr Leu Pro Gly Val Gln Ile Ser Phe Ser Asp Gly Arg Ala Lys 50 55 60 Leu Thr Ile Pro Ala Val Thr Lys Ala Asn Ser Gly Arg Tyr Ser Leu 65 70 75 80 Lys Ala Thr Asn Gly Ser Gly Gln Ala Thr Ser Thr Ala Glu Leu Leu 85 90 95 Val Lys Ala Glu Thr Ala Pro Pro Asn Phe Val Gln Arg Leu Gln Ser 100 105 110 Met Thr Val Arg Gln Gly Ser Gln Val Arg Leu Gln Val Arg Val Thr 115 120 125 Gly Ile Pro Thr Pro Val Val Lys Phe Tyr Arg Asp Gly Ala Glu Ile 130 135 140 Gln Ser Ser Leu Asp Phe Gln Ile Ser Gln Glu Gly Asp Leu Tyr Ser 145 150 155 160 Leu Leu Ile Ala Glu Ala Tyr Pro Glu Asp Ser Gly Thr Tyr Ser Val 165 170 175 Asn Ala Thr Asn Ser Val Gly Arg Ala Thr Ser Thr Ala Glu Leu Leu 180 185 190 Val Gln Gly Glu Glu Glu Val Pro Ala Lys Lys Thr Lys Thr Ile Val 195 200 205 Ser Thr Ala Gln Ile Ser Glu Ser Arg Gln Thr Arg Ile Glu Lys Lys 210 215 220 Ile Glu Ala His Phe Asp Ala Arg Ser Ile Ala Thr Val Glu Met Val 225 230 235 240 Ile Asp Gly Ala Ala Gly Gln Gln Leu Pro His Lys Thr Pro Pro Arg 245 250 255 Ile Pro Pro Lys Pro Lys Ser Arg Ser Pro Thr Pro Pro Ser Ile Ala 260 265 270 Ala Lys Ala Gln Leu Ala Arg Gln Gln Ser Pro Ser Pro Ile Arg His 275 280 285 Ser Pro Ser Pro Val Arg His Val Arg Ala Pro Thr Pro Ser Pro Val 290 295 300 Arg Ser Val Ser Pro Ala Ala Arg Ile Ser Thr Ser Pro Ile Arg Ser 305 310 315 320 Val Arg Ser Pro Leu Leu Met Arg Lys Thr Gln Ala Ser Thr Val Ala 325 330 335 Thr Gly Pro Glu Val Pro Pro Pro Trp Lys Gln Glu Gly Tyr Val Ala 340 345 350 Ser Ser Ser Glu Ala Glu Met Arg Glu Thr Thr Leu Thr Thr Ser Thr 355 360 365 Gln Ile Arg Thr Glu Glu Arg Trp Glu Gly Arg Tyr Gly Val Gln Glu 370 375 380 Gln Val Thr Ile Ser Gly Ala Ala Gly Ala Ala Ala Ser Val Ser Ala 385 390 395 400 Ser Ala Ser Tyr Ala Ala Glu Ala Val Ala Thr Gly Ala Lys Glu Val 405 410 415 Lys Gln Asp Ala Asp Lys Ser Ala Ala Val Ala Thr Val Val Ala Ala 420 425 430 Val Asp Met Ala Arg Val Arg Glu Pro Val Ile Ser Ala Val Glu Gln 435 440 445 Thr Ala Gln Arg Thr Thr Thr Thr Ala Val His Ile Gln Pro Ala Gln 450 455 460 Glu Gln Val Arg Lys Glu Ala Glu Lys Thr Ala Val Thr Lys Val Val 465 470 475 480 Val Ala Ala Asp Lys Ala Lys Glu Gln Glu Leu Lys Ser Arg Thr Lys 485 490 495 Glu Ile Ile Thr Thr Lys Gln Glu Gln Met His Val Thr His Glu Gln 500 505 510 Ile Arg Lys Glu Thr Glu Lys Thr Phe Val Pro Lys Val Val Ile Ser 515 520 525 Ala Ala Lys Ala Lys Glu Gln Glu Thr Arg Ile Ser Glu Glu Ile Thr 530 535 540 Lys Lys Gln Lys Gln Val Thr Gln Glu Ala Ile Met Lys Glu Thr Arg 545 550 555 560 Lys Thr Val Val Pro Lys Val Ile Val Ala Thr Pro Lys Val Lys Glu 565 570 575 Gln Asp Leu Val Ser Arg Gly Arg Glu Gly Ile Thr Thr Lys Arg Glu 580 585 590 Gln Val Gln Ile Thr Gln Glu Lys Met Arg Lys Glu Ala Glu Lys Thr 595 600 605 Ala Leu Ser Thr Ile Ala Val Ala Thr Ala Lys Ala Lys Glu Gln Glu 610 615 620 Thr Ile Leu Arg Thr Arg Glu Thr Met Ala Thr Arg Gln Glu Gln Ile 625 630 635 640 Gln Val Thr His Gly Lys Val Asp Val Gly Lys Lys Ala Glu Ala Val 645 650 655 Ala Thr Val Val Ala Ala Val Asp Gln Ala Arg Val Arg Glu Pro Arg 660 665 670 Glu Pro Gly His Leu Glu Glu Ser Tyr Ala Gln Gln Thr Thr Leu Glu 675 680 685 Tyr Gly Tyr Lys Glu Arg Ile Ser Ala Ala Lys Val Ala Glu Pro Pro 690 695 700 Gln Arg Pro Ala Ser Glu Pro His Val Val Pro Lys Ala Val Lys Pro 705 710 715 720 Arg Val Ile Gln Ala Pro Ser Glu Thr His Ile Lys Thr Thr Asp Gln 725 730 735 Lys Gly Met His Ile Ser Ser Gln Ile Lys Lys Thr Thr Asp Leu Thr 740 745 750 Thr Glu Arg Leu Val His Val Asp Lys Arg Pro Arg Thr Ala Ser Pro 755 760 765 His Phe Thr Val Ser Lys Ile Ser Val Pro Lys Thr Glu His Gly Tyr 770 775 780 Glu Ala Ser Ile Ala Gly Ser Ala Ile Ala Thr Leu Gln Lys Glu Leu 785 790 795 800 Ser Ala Thr Ser Ser Ala Gln Lys Ile Thr Lys Ser Val Lys Ala Pro 805 810 815 Thr Val Lys Pro Ser Glu Thr Arg Val Arg Ala Glu Pro Thr Pro Leu 820 825 830 Pro Gln Phe Pro Phe Ala Asp Thr Pro Asp Thr Tyr Lys Ser Glu Ala 835 840 845 Gly Val Glu Val Lys Lys Glu Val Gly Val Ser Ile Thr Gly Thr Thr 850 855 860 Val Arg Glu Glu Arg Phe Glu Val Leu His Gly Arg Glu Ala Lys Val 865 870 875 880 Thr Glu Thr Ala Arg Val Pro Ala Pro Val Glu Ile Pro Val Thr Pro 885 890 895 Pro Thr Leu Val Ser Gly Leu Lys Asn Val Thr Val Ile Glu Gly Glu 900 905 910 Ser Val Thr Leu Glu Cys His Ile Ser Gly Tyr Pro Ser Pro Thr Val 915 920 925 Thr Trp Tyr Arg Glu Asp Tyr Gln Ile Glu Ser Ser Ile Asp Phe Gln 930 935 940 Ile Thr Phe Gln Ser Gly Ile Ala Arg Leu Met Ile Arg Glu Ala Phe 945 950 955 960 Ala Glu Asp Ser Gly Arg Phe Thr Cys Ser Ala Val Asn Glu Ala Gly 965 970 975 Thr Val Ser Thr Ser Cys Tyr Leu Ala Val Gln Val Ser Glu Glu Phe 980 985 990 Glu Lys Glu Thr Thr Ala Val Thr Glu Lys Phe Thr Thr Glu Glu Lys 995 1000 1005 Arg Phe Val Glu Ser Arg Asp Val Val Met Thr Asp Thr Ser Leu Thr 1010 1015 1020 Glu Glu Gln Ala Gly Pro Gly Glu Pro Ala Ala Pro Tyr Phe Ile Thr 1025 1030 1035 1040 Lys Pro Val Val Gln Lys Leu Val Glu Gly Gly Ser Val Val Phe Gly 1045 1050 1055 Cys Gln Val Gly Gly Asn Pro Lys Pro His Val Tyr Trp Lys Lys Ser 1060 1065 1070 Gly Val Pro Leu Thr Thr Gly Tyr Arg Tyr Lys Val Ser Tyr Asn Lys 1075 1080 1085 Gln Thr Gly Glu Cys Lys Leu Val Ile Ser Met Thr Phe Ala Asp Asp 1090 1095 1100 Ala Gly Glu Tyr Thr Ile Val Val Arg Asn Lys His Gly Glu Thr Ser 1105 1110 1115 1120 Ala Ser Ala Ser Leu Leu Glu Glu Ala Asp Tyr Glu Leu Leu Met Lys 1125 1130 1135 Ser Gln Gln Glu Met Leu Tyr Gln Thr Gln Val Thr Ala Phe Val Gln 1140 1145 1150 Glu Pro Glu Val Gly Glu Thr Ala Pro Gly Phe Val Tyr Ser Glu Tyr 1155 1160 1165 Glu Lys Glu Tyr Glu Lys Glu Gln Ala Leu Ile Arg Lys Lys Met Ala 1170 1175 1180 Lys Asp Thr Val Val Val Arg Thr Tyr Val Glu Asp Gln Glu Phe His 1185 1190 1195 1200 Ile Ser Ser Phe Glu Glu Arg Leu Ile Lys Glu Ile Glu Tyr Arg Ile 1205 1210 1215 Ile Lys Thr Thr Leu Glu Glu Leu Leu Glu Glu Asp Gly Glu Glu Lys 1220 1225 1230 Met Ala Val Asp Ile Ser Glu Ser Glu Ala Val Glu Ser Gly Phe Asp 1235 1240 1245 Leu Arg Ile Lys Asn Tyr Arg Ile Leu Glu Gly Met Gly Val Thr Phe 1250 1255 1260 His Cys Lys Met Ser Gly Tyr Pro Leu Pro Lys Ile Ala Trp Tyr Lys 1265 1270 1275 1280 Asp Gly Lys Arg Ile Lys His Gly Glu Arg Tyr Gln Met Asp Phe Leu 1285 1290 1295 Gln Asp Gly Arg Ala Ser Leu Arg Ile Pro Val Val Leu Pro Glu Asp 1300 1305 1310 Glu Gly Ile Tyr Thr Ala Phe Ala Ser Asn Ile Lys Gly Asn Ala Ile 1315 1320 1325 Cys Ser Gly Lys Leu Tyr Val Glu Pro Ala Ala Pro Leu Gly Ala Pro 1330 1335 1340 Thr Tyr Ile Pro Thr Leu Glu Pro Val Ser Arg Ile Arg Ser Leu Ser 1345 1350 1355 1360 Pro Arg Ser Val Ser Arg Ser Pro Ile Arg Met Ser Pro Ala Arg Met 1365 1370 1375 Ser Pro Ala Arg Met Ser Pro Ala Arg Met Ser Pro Ala Arg Met Ser 1380 1385 1390 Pro Gly Arg Arg Leu Glu Glu Thr Asp Glu Ser Gln Leu Glu Arg Leu 1395 1400 1405 Tyr Lys Pro Val Phe Val Leu Lys Pro Val Ser Phe Lys Cys Leu Glu 1410 1415 1420 Gly Gln Thr Ala Arg Phe Asp Leu Lys Val Val Gly Arg Pro Met Pro 1425 1430 1435 1440 Glu Thr Phe Trp Phe His Asp Gly Gln Gln Ile Val Asn Asp Tyr Thr 1445 1450 1455 His Lys Val Val Ile Lys Glu Asp Gly Thr Gln Ser Leu Ile Ile Val 1460 1465 1470 Pro Ala Thr Pro Ser Asp Ser Gly Glu Trp Thr Val Val Ala Gln Asn 1475 1480 1485 Arg Ala Gly Arg Ser Ser Ile Ser Val Ile Leu Thr Val Glu Ala Val 1490 1495 1500 Glu His Gln Val Lys Pro Met Phe Val Glu Lys Leu Lys Asn Val Asn 1505 1510 1515 1520 Ile Lys Glu Gly Ser Arg Leu Glu Met Lys Val Arg Ala Thr Gly Asn 1525 1530 1535 Pro Asn Pro Asp Ile Val Trp Leu Lys Asn Ser Asp Ile Ile Val Pro 1540 1545 1550 His Lys Tyr Pro Lys Ile Arg Ile Glu Gly Thr Lys Gly Glu Ala Ala 1555 1560 1565 Leu Lys Ile Asp Ser Thr Val Ser Gln Asp Ser Ala Trp Tyr Thr Ala 1570 1575 1580 Thr Ala Ile Asn Lys Ala Gly Arg Asp Thr Thr Arg Cys Lys Val Asn 1585 1590 1595 1600 Val Glu Val Glu Phe Ala Glu Pro Glu Pro Glu Arg Lys Leu Ile Ile 1605 1610 1615 Pro Arg Gly Thr Tyr Arg Ala Lys Glu Ile Ala Ala Pro Glu Leu Glu 1620 1625 1630 Pro Leu His Leu Arg Tyr Gly Gln Glu Gln Trp Glu Glu Gly Asp Leu 1635 1640 1645 Tyr Asp Lys Glu Lys Gln Gln Lys Pro Phe Phe Lys Lys Lys Leu Thr 1650 1655 1660 Ser Leu Arg Leu Lys Arg Phe Gly Pro Ala His Phe Glu Cys Arg Leu 1665 1670 1675 1680 Thr Pro Ile Ser Asp Pro Thr Met Val Val Glu Trp Leu His Asp Gly 1685 1690 1695 Lys Pro Leu Glu Ala Ala Asn Arg Leu Arg Met Ile Asn Glu Phe Gly 1700 1705 1710 Tyr Cys Ser Leu Asp Tyr Gly Val Ala Tyr Ser Arg Asp Ser Gly Ile 1715 1720 1725 Ile Thr Cys Arg Ala Thr Asn Lys Tyr Gly Thr Asp His Thr Ser Ala 1730 1735 1740 Thr Leu Ile Val Lys Asp Glu Lys Ser Leu Val Glu Glu Ser Gln Leu 1745 1750 1755 1760 Pro Glu Gly Arg Lys Gly Leu Gln Arg Ile Glu Glu Leu Glu Arg Met 1765 1770 1775 Ala His Glu Gly Ala Leu Thr Gly Val Thr Thr Asp Gln Lys Glu Lys 1780 1785 1790 Gln Lys Pro Asp Ile Val Leu Tyr Pro Glu Pro Val Arg Val Leu Glu 1795 1800 1805 Gly Glu Thr Ala Arg Phe Arg Cys Arg Val Thr Gly Tyr Pro Gln Pro 1810 1815 1820 Lys Val Asn Trp Tyr Leu Asn Gly Gln Leu Ile Arg Lys Ser Lys Arg 1825 1830 1835 1840 Phe Arg Val Arg Tyr Asp Gly Ile His Tyr Leu Asp Ile Val Asp Cys 1845 1850 1855 Lys Ser Tyr Asp Thr Gly Glu Val Lys Val Thr Ala Glu Asn Pro Glu 1860 1865 1870 Gly Val Ile Glu His Lys Val Lys Leu Glu Ile Gln Gln Arg Glu Asp 1875 1880 1885 Phe Arg Ser Val Leu Arg Arg Ala Pro Glu Pro Arg Pro Glu Phe His 1890 1895 1900 Val His Glu Pro Gly Lys Leu Gln Phe Glu Val Gln Lys Val Asp Arg 1905 1910 1915 1920 Pro Val Asp Thr Thr Glu Thr Lys Glu Val Val Lys Leu Lys Arg Ala 1925 1930 1935 Glu Arg Ile Thr His Glu Lys Val Pro Glu Glu Ser Glu Glu Leu Arg 1940 1945 1950 Ser Lys Phe Lys Arg Arg Thr Glu Glu Gly Tyr Tyr Glu Ala Ile Thr 1955 1960 1965 Ala Val Glu Leu Lys Ser Arg Lys Lys Asp Glu Ser Tyr Glu Glu Leu 1970 1975 1980 Leu Arg Lys Thr Lys Asp Glu Leu Leu His Trp Thr Lys Glu Leu Thr 1985 1990 1995 2000 Glu Glu Glu Lys Lys Ala Leu Ala Glu Glu Gly Lys Ile Thr Ile Pro 2005 2010 2015 Thr Phe Lys Pro Asp Lys Ile Glu Leu Ser Pro Ser Met Glu Ala Pro 2020 2025 2030 Lys Ile Phe Glu Arg Ile Gln Ser Gln Thr Val Gly Gln Gly Ser Asp 2035 2040 2045 Ala His Phe Arg Val Arg Val Val Gly Lys Pro Asp Pro Glu Cys Glu 2050 2055 2060 Trp Tyr Lys Asn Gly Val Lys Ile Glu Arg Ser Asp Arg Ile Tyr Trp 2065 2070 2075 2080 Tyr Trp Pro Glu Asp Asn Val Cys Glu Leu Val Ile Arg Asp Val Thr 2085 2090 2095 Ala Glu Asp Ser Ala Ser Ile Met Val Lys Ala Ile Asn Ile Ala Gly 2100 2105 2110 Glu Thr Ser Ser His Ala Phe Leu Leu Val Gln Ala Lys Gln Leu Ile 2115 2120 2125 Thr Phe Thr Gln Glu Leu Gln Asp Val Val Ala Lys Glu Lys Asp Thr 2130 2135 2140 Met Ala Thr Phe Glu Cys Glu Thr Ser Glu Pro Phe Val Lys Val Lys 2145 2150 2155 2160 Trp Tyr Lys Asp Gly Met Glu Val His Glu Gly Asp Lys Tyr Arg Met 2165 2170 2175 His Ser Asp Arg Lys Val His Phe Leu Ser Ile Leu Thr Ile Asp Thr 2180 2185 2190 Ser Asp Ala Glu Asp Tyr Ser Cys Val Leu Val Glu Asp Glu Asn Val 2195 2200 2205 Lys Thr Thr Ala Lys Leu Ile Val Glu Gly Ala Val Val Glu Phe Val 2210 2215 2220 Lys Glu Leu Gln Asp Ile Glu Val Pro Glu Ser Tyr Ser Gly Glu Leu 2225 2230 2235 2240 Glu Cys Ile Val Ser Pro Glu Asn Ile Glu Gly Lys Trp Tyr His Asn 2245 2250 2255 Asp Val Glu Leu Lys Ser Asn Gly Lys Tyr Thr Ile Thr Ser Arg Arg 2260 2265 2270 Gly Arg Gln Asn Leu Thr Val Lys Asp Val Thr Lys Glu Asp Gln Gly 2275 2280 2285 Glu Tyr Ser Phe Val Ile Asp Gly Lys Lys Thr Thr Cys Lys Leu Lys 2290 2295 2300 Met Lys Pro Arg Pro Ile Ala Ile Leu Gln Gly Leu Ser Asp Gln Lys 2305 2310 2315 2320 Val Cys Glu Gly Asp Ile Val Gln Leu Glu Val Lys Val Ser Leu Glu 2325 2330 2335 Ser Val Glu Gly Val Trp Met Lys Asp Gly Gln Glu Val Gln Pro Ser 2340 2345 2350 Asp Arg Val His Ile Val Ile Asp Lys Gln Ser His Met Leu Leu Ile 2355 2360 2365 Glu Asp Met Thr Lys Glu Asp Ala Gly Asn Tyr Ser Phe Thr Ile Pro 2370 2375 2380 Ala Leu Gly Leu Ser Thr Ser Gly Arg Val Ser Val Tyr Ser Val Asp 2385 2390 2395 2400 Val Ile Thr Pro Leu Lys Asp Val Asn Val Ile Glu Gly Thr Lys Ala 2405 2410 2415 Val Leu Glu Cys Lys Val Ser Val Pro Asp Val Thr Ser Val Lys Trp 2420 2425 2430 Tyr Leu Asn Asp Glu Gln Ile Lys Pro Asp Asp Arg Val Gln Ala Ile 2435 2440 2445 Val Lys Gly Thr Lys Gln Arg Leu Val Ile Asn Arg Thr His Ala Ser 2450 2455 2460 Asp Glu Gly Pro Tyr Lys Leu Ile Val Gly Arg Val Glu Thr Asn Cys 2465 2470 2475 2480 Asn Leu Ser Val Glu Lys Ile Lys Ile Ile Arg Gly Leu Arg Asp Leu 2485 2490 2495 Thr Cys Thr Glu Thr Gln Asn Val Val Phe Glu Val Glu Leu Ser His 2500 2505 2510 Ser Gly Ile Asp Val Leu Trp Asn Phe Lys Asp Lys Glu Ile Lys Pro 2515 2520 2525 Ser Ser Lys Tyr Lys Ile Glu Ala His Gly Lys Ile Tyr Lys Leu Thr 2530 2535 2540 Val Leu Asn Met Met Lys Asp Asp Glu Gly Lys Tyr Thr Phe Tyr Ala 2545 2550 2555 2560 Gly Glu Asn Met Thr Ser Gly Lys Leu Thr Val Ala Gly Gly Ala Ile 2565 2570 2575 Ser Lys Pro Leu Thr Asp Gln Thr Val Ala Glu Ser Gln Glu Ala Val 2580 2585 2590 Phe Glu Cys Glu Val Ala Asn Pro Asp Ser Lys Gly Glu Trp Leu Arg 2595 2600 2605 Asp Gly Lys His Leu Pro Leu Thr Asn Asn Ile Arg Ser Glu Ser Asp 2610 2615 2620 Gly His Lys Arg Arg Leu Ile Ile Ala Ala Thr Lys Leu Asp Asp Ile 2625 2630 2635 2640 Gly Glu Tyr Thr Tyr Lys Val Ala Thr Ser Lys Thr Ser Ala Lys Leu 2645 2650 2655 Lys Val Glu Ala Val Lys Ile Lys Lys Thr Leu Lys Asn Leu Thr Val 2660 2665 2670 Thr Glu Thr Gln Asp Ala Val Phe Thr Val Glu Leu Thr His Pro Asn 2675 2680 2685 Val Lys Gly Val Gln Trp Ile Lys Asn Gly Val Val Leu Glu Ser Asn 2690 2695 2700 Glu Lys Tyr Ala Ile Ser Val Lys Gly Thr Ile Tyr Ser Leu Arg Ile 2705 2710 2715 2720 Lys Asn Cys Ala Ile Val Asp Glu Ser Val Tyr Gly Phe Arg Leu Gly 2725 2730 2735 Arg Leu Gly Ala Ser Ala Arg Leu His Val Glu Thr Val Lys Ile Ile 2740 2745 2750 Lys Lys Pro Lys Asp Val Thr Ala Leu Glu Asn Ala Thr Val Ala Phe 2755 2760 2765 Glu Val Ser Val Ser His Asp Thr Val Pro Val Lys Trp Phe His Lys 2770 2775 2780 Ser Val Glu Ile Lys Pro Ser Asp Lys His Arg Leu Val Ser Glu Arg 2785 2790 2795 2800 Lys Val His Lys Leu Met Leu Gln Asn Ile Ser Pro Ser Asp Ala Gly 2805 2810 2815 Glu Tyr Thr Ala Val Val Gly Gln Leu Glu Cys Lys Ala Lys Leu Phe 2820 2825 2830 Val Glu Thr Leu His Ile Thr Lys Thr Met Lys Asn Ile Glu Val Pro 2835 2840 2845 Glu Thr Lys Thr Ala Ser Phe Glu Cys Glu Val Ser His Phe Asn Val 2850 2855 2860 Pro Ser Met Trp Leu Lys Asn Gly Val Glu Ile Glu Met Ser Glu Lys 2865 2870 2875 2880 Phe Lys Ile Val Val Gln Gly Lys Leu His Gln Leu Ile Ile Met Asn 2885 2890 2895 Thr Ser Thr Glu Asp Ser Ala Glu Tyr Thr Phe Val Cys Gly Asn Asp 2900 2905 2910 Gln Val Ser Ala Thr Leu Thr Val Thr Pro Ile Met Ile Thr Ser Met 2915 2920 2925 Leu Lys Asp Ile Asn Ala Glu Glu Lys Asp Thr Ile Thr Phe Glu Val 2930 2935 2940 Thr Val Asn Tyr Glu Gly Ile Ser Tyr Lys Trp Leu Lys Asn Gly Val 2945 2950 2955 2960 Glu Ile Lys Ser Thr Asp Lys Cys Gln Met Arg Thr Lys Lys Leu Thr 2965 2970 2975 His Ser Leu Asn Ile Arg Asn Val His Phe Gly Asp Ala Ala Asp Tyr 2980 2985 2990 Thr Phe Val Ala Gly Lys Ala Thr Ser Thr Ala Thr Leu Tyr Val Glu 2995 3000 3005 Ala Arg His Ile Glu Phe Arg Lys His Ile Lys Asp Ile Lys Val Leu 3010 3015 3020 Glu Lys Lys Arg Ala Met Phe Glu Cys Glu Val Ser Glu Pro Asp Ile 3025 3030 3035 3040 Thr Val Gln Trp Met Lys Asp Asp Gln Glu Leu Gln Ile Thr Asp Arg 3045 3050 3055 Ile Lys Ile Gln Lys Glu Lys Tyr Val His Arg Leu Leu Ile Pro Ser 3060 3065 3070 Thr Arg Met Ser Asp Ala Gly Lys Tyr Thr Val Val Ala Gly Gly Asn 3075 3080 3085 Val Ser Thr Ala Lys Leu Phe Val Glu Gly Arg Asp Val Arg Ile Arg 3090 3095 3100 Ser Ile Lys Lys Glu Val Gln Val Ile Glu Lys Gln Arg Ala Val Val 3105 3110 3115 3120 Glu Phe Glu Val Asn Glu Asp Asp Val Asp Ala His Trp Tyr Lys Asp 3125 3130 3135 Gly Ile Glu Ile Asn Phe Gln Val Gln Glu Arg His Lys Tyr Val Val 3140 3145 3150 Glu Arg Arg Ile His Arg Met Phe Ile Ser Glu Thr Arg Gln Ser Asp 3155 3160 3165 Ala Gly Glu Tyr Thr Phe Val Ala Gly Arg Asn Arg Ser Ser Val Thr 3170 3175 3180 Leu Tyr Val Asn Ala Pro Glu Pro Pro Gln Val Leu Gln Glu Leu Gln 3185 3190 3195 3200 Pro Val Thr Val Gln Ser Gly Lys Pro Ala Arg Phe Cys Ala Met Ile 3205 3210 3215 Ser Gly Arg Pro Gln Pro Lys Ile Ser Trp Tyr Lys Glu Glu Gln Leu 3220 3225 3230 Leu Ser Thr Gly Phe Lys Cys Lys Phe Leu His Asp Gly Gln Glu Tyr 3235 3240 3245 Thr Leu Leu Leu Ile Glu Ala Phe Pro Glu Asp Ala Ala Val Tyr Thr 3250 3255 3260 Cys Glu Ala Lys Asn Asp Tyr Gly Val Ala Thr Thr Ser Ala Ser Leu 3265 3270 3275 3280 Ser Val Glu Val Pro Glu Val Val Ser Pro Asp Gln Glu Met Pro Val 3285 3290 3295 Tyr Pro Pro Ala Ile Ile Thr Pro Leu Gln Asp Thr Val Thr Ser Glu 3300 3305 3310 Gly Gln Pro Ala Arg Phe Gln Cys Arg Val Ser Gly Thr Asp Leu Lys 3315 3320 3325 Val Ser Trp Tyr Ser Lys Asp Lys Lys Ile Lys Pro Ser Arg Phe Phe 3330 3335 3340 Arg Met Thr Gln Phe Glu Asp Thr Tyr Gln Leu Glu Ile Ala Glu Ala 3345 3350 3355 3360 Tyr Pro Glu Asp Glu Gly Thr Tyr Thr Phe Val Ala Asn Asn Ala Val 3365 3370 3375 Gly Gln Val Ser Ser Thr Ala Asn Leu Ser Leu Glu Ala Pro Glu Ser 3380 3385 3390 Ile Leu His Glu Arg Ile Glu Gln Glu Ile Glu Met Glu Met Lys Glu 3395 3400 3405 Phe Ser Ser Ser Phe Leu Ser Ala Glu Glu Glu Gly Leu His Ser Ala 3410 3415 3420 Glu Leu Gln Leu Ser Lys Ile Asn Glu Thr Leu Glu Leu Leu Ser Glu 3425 3430 3435 3440 Ser Pro Val Tyr Pro Thr Lys Phe Asp Ser Glu Lys Glu Gly Thr Gly 3445 3450 3455 Pro Ile Phe Ile Lys Glu Val Ser Asn Ala Asp Ile Ser Met Gly Asp 3460 3465 3470 Val Ala Thr Leu Ser Val Thr Val Ile Gly Ile Pro Lys Pro Lys Ile 3475 3480 3485 Gln Trp Phe Phe Asn Gly Val Leu Leu Thr Pro Ser Ala Asp Tyr Lys 3490 3495 3500 Phe Val Phe Asp Gly Asp Asp His Ser Leu Ile Ile Leu Phe Thr Lys 3505 3510 3515 3520 Leu Glu Asp Glu Gly Glu Tyr Thr Cys Met Ala Ser Asn Asp Tyr Gly 3525 3530 3535 Lys Thr Ile Cys Ser Ala Tyr Leu Lys Ile Asn Ser Lys Gly Glu Gly 3540 3545 3550 His Lys Asp Thr Glu Thr Glu Ser Ala Val Ala Lys Ser Leu Glu Lys 3555 3560 3565 Leu Gly Gly Pro Cys Pro Pro His Phe Leu Lys Glu Leu Lys Pro Ile 3570 3575 3580 Arg Cys Ala Gln Gly Leu Pro Ala Ile Phe Glu Tyr Thr Val Val Gly 3585 3590 3595 3600 Glu Pro Ala Pro Thr Val Thr Trp Phe Lys Glu Asn Lys Gln Leu Cys 3605 3610 3615 Thr Ser Val Tyr Tyr Thr Ile Ile His Asn Pro Asn Gly Ser Gly Thr 3620 3625 3630 Phe Ile Val Asn Asp Pro Gln Arg Glu Asp Ser Gly Leu Tyr Ile Cys 3635 3640 3645 Lys Ala Glu Asn Met Leu Gly Glu Ser Thr Cys Ala Ala Glu Leu Leu 3650 3655 3660 Val Leu Leu Glu Asp Thr Asp Met Thr Asp Thr Pro Cys Lys Ala Lys 3665 3670 3675 3680 Ser Thr Pro Glu Ala Pro Glu Asp Phe Pro Gln Thr Pro Leu Lys Gly 3685 3690 3695 Pro Ala Val Glu Ala Leu Asp Ser Glu Gln Glu Ile Ala Thr Phe Val 3700 3705 3710 Lys Asp Thr Ile Leu Lys Ala Ala Leu Ile Thr Glu Glu Asn Gln Gln 3715 3720 3725 Leu Ser Tyr Glu His Ile Ala Lys Ala Asn Glu Leu Ser Ser Gln Leu 3730 3735 3740 Pro Leu Gly Ala Gln Glu Leu Gln Ser Ile Leu Glu Gln Asp Lys Leu 3745 3750 3755 3760 Thr Pro Glu Ser Thr Arg Glu Phe Leu Cys Ile Asn Gly Ser Ile His 3765 3770 3775 Phe Gln Pro Leu Lys Glu Pro Ser Pro Asn Leu Gln Leu Gln Ile Val 3780 3785 3790 Gln Ser Gln Lys Thr Phe Ser Lys Glu Gly Ile Leu Met Pro Glu Glu 3795 3800 3805 Pro Glu Thr Gln Ala Val Leu Ser Asp Thr Glu Lys Ile Phe Pro Ser 3810 3815 3820 Ala Met Ser Ile Glu Gln Ile Asn Ser Leu Thr Val Glu Pro Leu Lys 3825 3830 3835 3840 Thr Leu Leu Ala Glu Pro Glu Gly Asn Tyr Pro Gln Ser Ser Ile Glu 3845 3850 3855 Pro Pro Met His Ser Tyr Leu Thr Ser Val Ala Glu Glu Val Leu Ser 3860 3865 3870 Leu Lys Glu Lys Thr Val Ser Asp Thr Asn Arg Glu Gln Arg Val Thr 3875 3880 3885 Leu Gln Lys Gln Glu Ala Gln Ser Ala Leu Ile Leu Ser Gln Ser Leu 3890 3895 3900 Ala Glu Gly His Val Glu Ser Leu Gln Ser Pro Asp Val Met Ile Ser 3905 3910 3915 3920 Gln Val Asn Tyr Glu Pro Leu Val Pro Ser Glu His Ser Cys Thr Glu 3925 3930 3935 Gly Gly Lys Ile Leu Ile Glu Ser Ala Asn Pro Leu Glu Asn Ala Gly 3940 3945 3950 Gln Asp Ser Ala Val Arg Ile Glu Glu Gly Lys Ser Leu Arg Phe Pro 3955 3960 3965 Leu Ala Leu Glu Glu Lys Gln Val Leu Leu Lys Glu Glu His Ser Asp 3970 3975 3980 Asn Val Val Met Pro Pro Asp Gln Ile Ile Glu Ser Lys Arg Glu Pro 3985 3990 3995 4000 Val Ala Ile Lys Lys Val Gln Glu Val Gln Gly Arg Asp Leu Leu Ser 4005 4010 4015 Lys Glu Ser Leu Leu Ser Gly Ile Pro Glu Glu Gln Arg Leu Asn Leu 4020 4025 4030 Lys Ile Gln Ile Cys Arg Ala Leu Gln Ala Ala Val Ala Ser Glu Gln 4035 4040 4045 Pro Gly Leu Phe Ser Glu Trp Leu Arg Asn Ile Glu Lys Val Glu Val 4050 4055 4060 Glu Ala Val Asn Ile Thr Gln Glu Pro Arg His Ile Met Cys Met Tyr 4065 4070 4075 4080 Leu Val Thr Ser Ala Lys Ser Val Thr Glu Glu Val Thr Ile Ile Ile 4085 4090 4095 Glu Asp Val Asp Pro Gln Met Ala Asn Leu Lys Met Glu Leu Arg Asp 4100 4105 4110 Ala Leu Cys Ala Ile Ile Tyr Glu Glu Ile Asp Ile Leu Thr Ala Glu 4115 4120 4125 Gly Pro Arg Ile Gln Gln Gly Ala Lys Thr Ser Leu Gln Glu Glu Met 4130 4135 4140 Asp Ser Phe Ser Gly Ser Gln Lys Val Glu Pro Ile Thr Glu Pro Glu 4145 4150 4155 4160 Val Glu Ser Lys Tyr Leu Ile Ser Thr Glu Glu Val Ser Tyr Phe Asn 4165 4170 4175 Val Gln Ser Arg Val Lys Tyr Leu Asp Ala Thr Pro Val Thr Lys Gly 4180 4185 4190 Val Ala Ser Ala Val Val Ser Asp Glu Lys Gln Asp Glu Ser Leu Lys 4195 4200 4205 Pro Ser Glu Glu Lys Glu Glu Ser Ser Ser Glu Ser Gly Thr Glu Glu 4210 4215 4220 Val Ala Thr Val Lys Ile Gln Glu Ala Glu Gly Gly Leu Ile Lys Glu 4225 4230 4235 4240 Asp Gly Pro Met Ile His Thr Pro Leu Val Asp Thr Val Ser Glu Glu 4245 4250 4255 Gly Asp Ile Val His Leu Thr Thr Ser Ile Thr Asn Ala Lys Glu Val 4260 4265 4270 Asn Trp Tyr Phe Glu Asn Lys Leu Val Pro Ser Asp Glu Lys Phe Lys 4275 4280 4285 Cys Leu Gln Asp Gln Asn Thr Tyr Thr Leu Val Ile Asp Lys Val Asn 4290 4295 4300 Thr Glu Asp His Gln Gly Glu Tyr Val Cys Glu Ala Leu Asn Asp Ser 4305 4310 4315 4320 Gly Lys Thr Ala Thr Ser Ala Lys Leu Thr Val Val Lys Arg Ala Ala 4325 4330 4335 Pro Val Ile Lys Arg Lys Ile Glu Pro Leu Glu Val Ala Leu Gly His 4340 4345 4350 Leu Ala Lys Phe Thr Cys Glu Ile Gln Ser Ala Pro Asn Val Arg Phe 4355 4360 4365 Gln Trp Phe Lys Ala Gly Arg Glu Ile Tyr Glu Ser Asp Lys Cys Ser 4370 4375 4380 Ile Arg Ser Ser Lys Tyr Ile Ser Ser Leu Glu Ile Leu Arg Thr Gln 4385 4390 4395 4400 Val Val Asp Cys Gly Glu Tyr Thr Cys Lys Ala Ser Asn Glu Tyr Gly 4405 4410 4415 Ser Val Ser Cys Thr Ala Thr Leu Thr Val Thr Val Pro Gly Gly Glu 4420 4425 4430 Lys Lys Val Arg Lys Leu Leu Pro Glu Arg Lys Pro Glu Pro Lys Glu 4435 4440 4445 Glu Val Val Leu Lys Ser Val Leu Arg Lys Arg Pro Glu Glu Glu Glu 4450 4455 4460 Pro Lys Val Glu Pro Lys Lys Leu Glu Lys Val Lys Lys Pro Ala Val 4465 4470 4475 4480 Pro Glu Pro Pro Pro Pro Lys Pro Val Glu Glu Val Glu Val Pro Thr 4485 4490 4495 Val Thr Lys Arg Glu Arg Lys Ile Pro Glu Pro Thr Lys Val Pro Glu 4500 4505 4510 Ile Lys Pro Ala Ile Pro Leu Pro Ala Pro Glu Pro Lys Pro Lys Pro 4515 4520 4525 Glu Ala Glu Val Lys Thr Ile Lys Pro Pro Pro Val Glu Pro Glu Pro 4530 4535 4540 Thr Pro Ile Ala Ala Pro Val Thr Val Pro Val Val Gly Lys Lys Ala 4545 4550 4555 4560 Glu Ala Lys Ala Pro Lys Glu Glu Ala Ala Lys Pro Lys Gly Pro Ile 4565 4570 4575 Lys Gly Val Pro Lys Lys Thr Pro Ser Pro Ile Glu Ala Glu Arg Arg 4580 4585 4590 Lys Leu Arg Pro Gly Ser Gly Gly Glu Lys Pro Pro Asp Glu Ala Pro 4595 4600 4605 Phe Thr Tyr Gln Leu Lys Ala Val Pro Leu Lys Phe Val Lys Glu Ile 4610 4615 4620 Lys Asp Ile Ile Leu Thr Glu Ser Glu Phe Val Gly Ser Ser Ala Ile 4625 4630 4635 4640 Phe Glu Cys Leu Val Ser Pro Ser Thr Ala Ile Thr Thr Trp Met Lys 4645 4650 4655 Asp Gly Ser Asn Ile Arg Glu Ser Pro Lys His Arg Phe Ile Ala Asp 4660 4665 4670 Gly Lys Asp Arg Lys Leu His Ile Ile Asp Val Gln Leu Ser Asp Ala 4675 4680 4685 Gly Glu Tyr Thr Cys Val Leu Arg Leu Gly Asn Lys Glu Lys Thr Ser 4690 4695 4700 Thr Ala Lys Leu Val Val Glu Glu Leu Pro Val Arg Phe Val Lys Thr 4705 4710 4715 4720 Leu Glu Glu Glu Val Thr Val Val Lys Gly Gln Pro Leu Tyr Leu Ser 4725 4730 4735 Cys Glu Leu Asn Lys Glu Arg Asp Val Val Trp Arg Lys Asp Gly Lys 4740 4745 4750 Ile Val Val Glu Lys Pro Gly Arg Ile Val Pro Gly Val Ile Gly Leu 4755 4760 4765 Met Arg Ala Leu Thr Ile Asn Asp Ala Asp Asp Thr Asp Ala Gly Thr 4770 4775 4780 Tyr Thr Val Thr Val Glu Asn Ala Asn Asn Leu Glu Cys Ser Ser Cys 4785 4790 4795 4800 Val Lys Val Val Glu Val Ile Arg Asp Trp Leu Val Lys Pro Ile Arg 4805 4810 4815 Asp Gln His Val Lys Pro Lys Gly Thr Ala Ile Phe Ala Cys Asp Ile 4820 4825 4830 Ala Lys Asp Thr Pro Asn Ile Lys Trp Phe Lys Gly Tyr Asp Glu Ile 4835 4840 4845 Pro Ala Glu Pro Asn Asp Lys Thr Glu Ile Leu Arg Asp Gly Asn His 4850 4855 4860 Leu Tyr Leu Lys Ile Lys Asn Ala Met Pro Glu Asp Ile Ala Glu Tyr 4865 4870 4875 4880 Ala Val Glu Ile Glu Gly Lys Arg Tyr Pro Ala Lys Leu Thr Leu Gly 4885 4890 4895 Glu Arg Glu Val Glu Leu Leu Lys Pro Ile Glu Asp Val Thr Ile Tyr 4900 4905 4910 Glu Lys Glu Ser Ala Ser Phe Asp Ala Glu Ile Ser Glu Ala Asp Ile 4915 4920 4925 Pro Gly Gln Trp Lys Leu Lys Gly Glu Leu Leu Arg Pro Ser Pro Thr 4930 4935 4940 Cys Glu Ile Lys Ala Glu Gly Gly Lys Arg Phe Leu Thr Leu His Lys 4945 4950 4955 4960 Val Lys Leu Asp Gln Ala Gly Glu Val Leu Tyr Gln Ala Leu Asn Ala 4965 4970 4975 Ile Thr Thr Ala Ile Leu Thr Val Lys Glu Ile Glu Leu Asp Phe Ala 4980 4985 4990 Val Pro Leu Lys Asp Val Thr Val Pro Glu Arg Arg Gln Ala Arg Phe 4995 5000 5005 Glu Cys Val Leu Thr Arg Glu Ala Asn Val Ile Trp Ser Lys Gly Pro 5010 5015 5020 Asp Ile Ile Lys Ser Ser Asp Lys Phe Asp Ile Ile Ala Asp Gly Lys 5025 5030 5035 5040 Lys His Ile Leu Val Ile Asn Asp Ser Gln Phe Asp Asp Glu Gly Val 5045 5050 5055 Tyr Thr Ala Glu Val Glu Gly Lys Lys Thr Ser Ala Arg Leu Phe Val 5060 5065 5070 Thr Gly Ile Arg Leu Lys Phe Met Ser Pro Leu Glu Asp Gln Thr Val 5075 5080 5085 Lys Glu Gly Glu Thr Ala Thr Phe Val Cys Glu Leu Ser His Glu Lys 5090 5095 5100 Met His Val Val Trp Phe Lys Asn Asp Ala Lys Leu His Thr Ser Arg 5105 5110 5115 5120 Thr Val Leu Ile Ser Ser Glu Gly Lys Thr His Lys Leu Glu Met Lys 5125 5130 5135 Glu Val Thr Leu Asp Asp Ile Ser Gln Ile Lys Ala Gln Val Lys Glu 5140 5145 5150 Leu Ser Ser Thr Ala Gln Leu Lys Val Leu Glu Ala Asp Pro Tyr Phe 5155 5160 5165 Thr Val Lys Leu His Asp Lys Thr Ala Val Glu Lys Asp Glu Ile Thr 5170 5175 5180 Leu Lys Cys Glu Val Ser Lys Asp Val Pro Val Lys Trp Phe Lys Asp 5185 5190 5195 5200 Gly Glu Glu Ile Val Pro Ser Pro Lys Tyr Ser Ile Lys Ala Asp Gly 5205 5210 5215 Leu Arg Arg Ile Leu Lys Ile Lys Lys Ala Asp Leu Lys Asp Lys Gly 5220 5225 5230 Glu Tyr Val Cys Asp Cys Gly Thr Asp Lys Thr Lys Ala Asn Val Thr 5235 5240 5245 Val Glu Ala Arg Leu Ile Glu Val Glu Lys Pro Leu Tyr Gly Val Glu 5250 5255 5260 Val Phe Val Gly Glu Thr Ala His Phe Glu Ile Glu Leu Ser Glu Pro 5265 5270 5275 5280 Asp Val His Gly Gln Trp Lys Leu Lys Gly Gln Pro Leu Thr Ala Ser 5285 5290 5295 Pro Asp Cys Glu Ile Ile Glu Asp Gly Lys Lys His Ile Leu Ile Leu 5300 5305 5310 His Asn Cys Gln Leu Gly Met Thr Gly Glu Val Ser Phe Gln Ala Ala 5315 5320 5325 Asn Ala Lys Ser Ala Ala Asn Leu Lys Val Lys Glu Leu Pro Leu Ile 5330 5335 5340 Phe Ile Thr Pro Leu Ser Asp Val Lys Val Phe Glu Lys Asp Glu Ala 5345 5350 5355 5360 Lys Phe Glu Cys Glu Val Ser Arg Glu Pro Lys Thr Phe Arg Trp Leu 5365 5370 5375 Lys Gly Thr Gln Glu Ile Thr Gly Asp Asp Arg Phe Glu Leu Ile Lys 5380 5385 5390 Asp Gly Thr Lys His Ser Met Val Ile Lys Ser Ala Ala Phe Glu Asp 5395 5400 5405 Glu Ala Lys Tyr Met Phe Glu Ala Glu Asp Lys His Thr Ser Gly Lys 5410 5415 5420 Leu Ile Ile Glu Gly Ile Arg Leu Lys Phe Leu Thr Pro Leu Lys Asp 5425 5430 5435 5440 Val Thr Ala Lys Glu Lys Glu Ser Ala Val Phe Thr Val Glu Leu Ser 5445 5450 5455 His Asp Asn Ile Arg Val Lys Trp Phe Lys Asn Asp Gln Arg Leu His 5460 5465 5470 Thr Thr Arg Ser Val Ser Met Gln Asp Glu Gly Lys Thr His Ser Ile 5475 5480 5485 Thr Phe Lys Asp Leu Ser Ile Asp Asp Thr Ser Gln Ile Arg Val Glu 5490 5495 5500 Ala Met Gly Met Ser Ser Glu Ala Lys Leu Thr Val Leu Glu Gly Asp 5505 5510 5515 5520 Pro Tyr Phe Thr Gly Lys Leu Gln Asp Tyr Thr Gly Val Glu Lys Asp 5525 5530 5535 Glu Val Ile Leu Gln Cys Glu Ile Ser Lys Ala Asp Ala Pro Val Lys 5540 5545 5550 Trp Phe Lys Asp Gly Lys Glu Ile Lys Pro Ser Lys Asn Ala Val Ile 5555 5560 5565 Lys Thr Asp Gly Lys Lys Arg Met Leu Ile Leu Lys Lys Ala Leu Lys 5570 5575 5580 Ser Asp Ile Gly Gln Tyr Thr Cys Asp Cys Gly Thr Asp Lys Thr Ser 5585 5590 5595 5600 Gly Lys Leu Asp Ile Glu Asp Arg Glu Ile Lys Leu Val Arg Pro Leu 5605 5610 5615 His Ser Val Glu Val Met Glu Thr Glu Thr Ala Arg Phe Glu Thr Glu 5620 5625 5630 Ile Ser Glu Asp Asp Ile His Ala Asn Trp Lys Leu Lys Gly Glu Ala 5635 5640 5645 Leu Leu Gln Thr Pro Asp Cys Glu Ile Lys Glu Glu Gly Lys Ile His 5650 5655 5660 Ser Leu Val Leu His Asn Cys Arg Leu Asp Gln Thr Gly Gly Val Asp 5665 5670 5675 5680 Phe Gln Ala Ala Asn Val Lys Ser Ser Ala His Leu Arg Val Lys Pro 5685 5690 5695 Arg Val Ile Gly Leu Leu Arg Pro Leu Lys Asp Val Thr Val Thr Ala 5700 5705 5710 Gly Glu Thr Ala Thr Phe Asp Cys Glu Leu Ser Tyr Glu Asp Ile Pro 5715 5720 5725 Val Glu Trp Tyr Leu Lys Gly Lys Lys Leu Glu Pro Ser Asp Lys Val 5730 5735 5740 Val Pro Arg Ser Glu Gly Lys Val His Thr Leu Thr Leu Arg Asp Val 5745 5750 5755 5760 Lys Leu Glu Asp Ala Gly Glu Val Gln Leu Thr Ala Lys Asp Phe Lys 5765 5770 5775 Thr His Ala Asn Leu Phe Val Lys Glu Pro Pro Val Glu Phe Thr Lys 5780 5785 5790 Pro Leu Glu Asp Gln Thr Val Glu Glu Gly Ala Thr Ala Val Leu Glu 5795 5800 5805 Cys Glu Val Ser Arg Glu Asn Ala Lys Val Lys Trp Phe Lys Asn Gly 5810 5815 5820 Thr Glu Ile Leu Lys Ser Lys Lys Tyr Glu Ile Val Ala Asp Gly Arg 5825 5830 5835 5840 Val Arg Lys Leu Val Ile His Asp Cys Thr Pro Glu Asp Ile Lys Thr 5845 5850 5855 Tyr Thr Cys Asp Ala Lys Asp Phe Lys Thr Ser Cys Asn Leu Asn Val 5860 5865 5870 Val Pro Pro His Val Glu Phe Leu Arg Pro Leu Thr Asp Leu Gln Val 5875 5880 5885 Arg Glu Lys Glu Met Ala Arg Phe Glu Cys Glu Leu Ser Arg Glu Asn 5890 5895 5900 Ala Lys Val Lys Trp Phe Lys Asp Gly Ala Glu Ile Lys Lys Gly Lys 5905 5910 5915 5920 Lys Tyr Asp Ile Ile Ser Lys Gly Ala Val Arg Ile Leu Val Ile Asn 5925 5930 5935 Lys Cys Leu Leu Asp Asp Glu Ala Glu Tyr Ser Cys Glu Val Arg Thr 5940 5945 5950 Ala Arg Thr Ser Gly Met Leu Thr Val Leu Glu Glu Glu Ala Val Phe 5955 5960 5965 Thr Lys Asn Leu Ala Asn Ile Glu Val Ser Glu Thr Asp Thr Ile Lys 5970 5975 5980 Leu Val Cys Glu Val Ser Lys Pro Gly Ala Glu Val Ile Trp Tyr Lys 5985 5990 5995 6000 Gly Asp Glu Glu Ile Ile Glu Thr Gly Arg Tyr Glu Ile Leu Thr Glu 6005 6010 6015 Gly Arg Lys Arg Ile Leu Val Ile Gln Asn Ala His Leu Glu Asp Ala 6020 6025 6030 Gly Asn Tyr Asn Cys Arg Leu Pro Ser Ser Arg Thr Asp Gly Lys Val 6035 6040 6045 Lys Val His Glu Leu Ala Ala Glu Phe Ile Ser Lys Pro Gln Asn Leu 6050 6055 6060 Glu Ile Leu Glu Gly Glu Lys Ala Glu Phe Val Cys Ser Ile Ser Lys 6065 6070 6075 6080 Glu Ser Phe Pro Val Gln Trp Lys Arg Asp Asp Lys Thr Leu Glu Ser 6085 6090 6095 Gly Asp Lys Tyr Asp Val Ile Ala Asp Gly Lys Lys Arg Val Leu Val 6100 6105 6110 Val Lys Asp Ala Thr Leu Gln Asp Met Gly Thr Tyr Val Val Met Val 6115 6120 6125 Gly Ala Ala Arg Ala Ala Ala His Leu Thr Val Ile Glu Lys Leu Arg 6130 6135 6140 Ile Val Val Pro Leu Lys Asp Thr Arg Val Lys Glu Gln Gln Glu Val 6145 6150 6155 6160 Val Phe Asn Cys Glu Val Asn Thr Glu Gly Ala Lys Ala Lys Trp Phe 6165 6170 6175 Arg Asn Glu Glu Ala Ile Phe Asp Ser Ser Lys Tyr Ile Ile Leu Gln 6180 6185 6190 Lys Asp Leu Val Tyr Thr Leu Arg Ile Arg Asp Ala His Leu Asp Asp 6195 6200 6205 Gln Ala Asn Tyr Asn Val Ser Leu Thr Asn His Arg Gly Glu Asn Val 6210 6215 6220 Lys Ser Ala Ala Asn Leu Ile Val Glu Glu Glu Asp Leu Arg Ile Val 6225 6230 6235 6240 Glu Pro Leu Lys Asp Ile Glu Thr Met Glu Lys Lys Ser Val Thr Phe 6245 6250 6255 Trp Cys Lys Val Asn Arg Leu Asn Val Thr Leu Lys Trp Thr Lys Asn 6260 6265 6270 Gly Glu Glu Val Pro Phe Asp Asn Arg Val Ser Tyr Arg Val Asp Lys 6275 6280 6285 Tyr Lys His Met Leu Thr Ile Lys Asp Cys Gly Phe Pro Asp Glu Gly 6290 6295 6300 Glu Tyr Ile Val Thr Ala Gly Gln Asp Lys Ser Val Ala Glu Leu Leu 6305 6310 6315 6320 Ile Ile Glu Ala Pro Thr Glu Phe Val Glu His Leu Glu Asp Gln Thr 6325 6330 6335 Val Thr Glu Phe Asp Asp Ala Val Phe Ser Cys Gln Leu Ser Arg Glu 6340 6345 6350 Lys Ala Asn Val Lys Trp Tyr Arg Asn Gly Arg Glu Ile Lys Glu Gly 6355 6360 6365 Lys Lys Tyr Lys Phe Glu Lys Asp Gly Ser Ile His Arg Leu Ile Ile 6370 6375 6380 Lys Asp Cys Arg Leu Asp Asp Glu Cys Glu Tyr Ala Cys Gly Val Glu 6385 6390 6395 6400 Asp Arg Lys Ser Arg Ala Arg Leu Phe Val Glu Glu Ile Pro Val Glu 6405 6410 6415 Ile Ile Arg Pro Pro Gln Asp Ile Leu Glu Ala Pro Gly Ala Asp Val 6420 6425 6430 Val Phe Leu Ala Glu Leu Asn Lys Asp Lys Val Glu Val Gln Trp Leu 6435 6440 6445 Arg Asn Asn Met Val Val Val Gln Gly Asp Lys His Gln Met Met Ser 6450 6455 6460 Glu Gly Lys Ile His Arg Leu Gln Ile Cys Asp Ile Lys Pro Arg Asp 6465 6470 6475 6480 Gln Gly Glu Tyr Arg Phe Ile Ala Lys Asp Lys Glu Ala Arg Ala Lys 6485 6490 6495 Leu Glu Leu Ala Ala Ala Pro Lys Ile Lys Thr Ala Asp Gln Asp Leu 6500 6505 6510 Val Val Asp Val Gly Lys Pro Leu Thr Met Val Val Pro Tyr Asp Ala 6515 6520 6525 Tyr Pro Lys Ala Glu Ala Glu Trp Phe Lys Glu Asn Glu Pro Leu Ser 6530 6535 6540 Thr Lys Thr Ile Asp Thr Thr Ala Glu Gln Thr Ser Phe Arg Ile Leu 6545 6550 6555 6560 Glu Ala Lys Lys Gly Asp Lys Gly Arg Tyr Lys Ile Val Leu Gln Asn 6565 6570 6575 Lys His Gly Lys Ala Glu Gly Phe Ile Asn Leu Lys Val Ile Asp Val 6580 6585 6590 Pro Gly Pro Val Arg Asn Leu Glu Val Thr Glu Thr Phe Asp Gly Glu 6595 6600 6605 Val Ser Leu Ala Trp Glu Glu Pro Leu Thr Asp Gly Gly Ser Lys Ile 6610 6615 6620 Ile Gly Tyr Val Val Glu Arg Arg Asp Ile Lys Arg Lys Thr Trp Val 6625 6630 6635 6640 Leu Ala Thr Asp Arg Ala Glu Ser Cys Glu Phe Thr Val Thr Gly Leu 6645 6650 6655 Gln Lys Gly Gly Val Glu Tyr Leu Phe Arg Val Ser Ala Arg Asn Arg 6660 6665 6670 Val Gly Thr Gly Glu Pro Val Glu Thr Asp Asn Pro Val Glu Ala Arg 6675 6680 6685 Ser Lys Tyr Asp Val Pro Gly Pro Pro Leu Asn Val Thr Ile Thr Asp 6690 6695 6700 Val Asn Arg Phe Gly Val Ser Leu Thr Trp Glu Pro Pro Glu Tyr Asp 6705 6710 6715 6720 Gly Gly Ala Glu Ile Thr Asn Tyr Val Ile Glu Leu Arg Asp Lys Thr 6725 6730 6735 Ser Ile Arg Trp Asp Thr Ala Met Thr Val Arg Ala Glu Asp Leu Ser 6740 6745 6750 Ala Thr Val Thr Asp Val Val Glu Gly Gln Glu Tyr Ser Phe Arg Val 6755 6760 6765 Arg Ala Gln Asn Arg Ile Gly Val Gly Lys Pro Ser Ala Ala Thr Pro 6770 6775 6780 Phe Val Lys Val Ala Asp Pro Ile Glu Arg Pro Ser Pro Pro Val Asn 6785 6790 6795 6800 Leu Thr Ser Ser Asp Gln Thr Gln Ser Ser Val Gln Leu Lys Trp Glu 6805 6810 6815 Pro Pro Leu Lys Asp Gly Gly Ser Pro Ile Leu Gly Tyr Ile Ile Glu 6820 6825 6830 Arg Cys Glu Glu Gly Lys Asp Asn Trp Ile Arg Cys Asn Met Lys Leu 6835 6840 6845 Val Pro Glu Leu Thr Tyr Lys Val Thr Gly Leu Glu Lys Gly Asn Lys 6850 6855 6860 Tyr Leu Tyr Arg Val Ser Ala Glu Asn Lys Ala Gly Val Ser Asp Pro 6865 6870 6875 6880 Ser Glu Ile Leu Gly Pro Leu Thr Ala Asp Asp Ala Phe Val Glu Pro 6885 6890 6895 Thr Met Asp Leu Ser Ala Phe Lys Asp Gly Leu Glu Val Ile Val Pro 6900 6905 6910 Asn Pro Ile Thr Ile Leu Val Pro Ser Thr Gly Tyr Pro Arg Pro Thr 6915 6920 6925 Ala Thr Trp Cys Phe Gly Asp Lys Val Leu Glu Thr Gly Asp Arg Val 6930 6935 6940 Lys Met Lys Thr Leu Ser Ala Tyr Ala Glu Leu Val Ile Ser Pro Ser 6945 6950 6955 6960 Glu Arg Ser Asp Lys Gly Ile Tyr Thr Leu Lys Leu Glu Asn Arg Val 6965 6970 6975 Lys Thr Ile Ser Gly Glu Ile Asp Val Asn Val Ile Ala Arg Pro Ser 6980 6985 6990 Ala Pro Lys Glu Leu Lys Phe Gly Asp Ile Thr Lys Asp Ser Val His 6995 7000 7005 Leu Thr Trp Glu Pro Pro Asp Asp Asp Gly Gly Ser Pro Leu Thr Gly 7010 7015 7020 Tyr Val Val Glu Lys Arg Glu Val Ser Arg Lys Thr Trp Thr Lys Val 7025 7030 7035 7040 Met Asp Phe Val Thr Asp Leu Glu Phe Thr Val Pro Asp Leu Val Gln 7045 7050 7055 Gly Lys Glu Tyr Leu Phe Lys Val Cys Ala Arg Asn Lys Cys Gly Pro 7060 7065 7070 Gly Glu Pro Ala Tyr Val Asp Glu Pro Val Asn Met Ser Thr Pro Ala 7075 7080 7085 Thr Val Pro Asp Pro Pro Glu Asn Val Lys Trp Arg Asp Arg Thr Ala 7090 7095 7100 Asn Ser Ile Phe Leu Thr Trp Asp Pro Pro Lys Asn Asp Gly Gly Ser 7105 7110 7115 7120 Arg Ile Lys Gly Tyr Ile Val Glu Arg Cys Pro Arg Gly Ser Asp Lys 7125 7130 7135 Trp Val Ala Cys Gly Glu Pro Val Ala Glu Thr Lys Met Glu Val Thr 7140 7145 7150 Gly Leu Glu Glu Gly Lys Trp Tyr Ala Tyr Arg Val Lys Thr Leu Asn 7155 7160 7165 Arg Gln Gly Ala Ser Lys Pro Ser Arg Pro Thr Glu Glu Ile Gln Ala 7170 7175 7180 Val Asp Thr Gln Glu Ala Pro Glu Ile Phe Leu Asp Val Lys Leu Leu 7185 7190 7195 7200 Ala Gly Leu Thr Val Lys Ala Gly Thr Lys Ile Glu Leu Pro Ala Thr 7205 7210 7215 Val Thr Gly Lys Pro Glu Pro Lys Ile Thr Trp Thr Lys Ala Asp Met 7220 7225 7230 Ile Leu Lys Gln Asp Lys Arg Ile Thr Ile Glu Asn Val Pro Lys Lys 7235 7240 7245 Ser Thr Val Thr Ile Val Asp Ser Lys Arg Ser Asp Thr Gly Thr Tyr 7250 7255 7260 Ile Ile Glu Ala Val Asn Val Cys Gly Arg Ala Thr Ala Val Val Glu 7265 7270 7275 7280 Val Asn Val Leu Asp Lys Pro Gly Pro Pro Ala Ala Phe Asp Ile Thr 7285 7290 7295 Asp Val Thr Asn Glu Ser Cys Leu Leu Thr Trp Asn Pro Pro Arg Asp 7300 7305 7310 Asp Gly Gly Ser Lys Ile Thr Asn Tyr Val Val Glu Arg Arg Ala Thr 7315 7320 7325 Asp Ser Glu Val Trp His Lys Leu Ser Ser Thr Val Lys Asp Thr Asn 7330 7335 7340 Phe Lys Ala Thr Lys Leu Ile Pro Asn Lys Glu Tyr Ile Phe Arg Val 7345 7350 7355 7360 Ala Ala Glu Asn Met Tyr Gly Ala Gly Glu Pro Val Gln Ala Ser Pro 7365 7370 7375 Ile Thr Ala Lys Tyr Gln Phe Asp Pro Pro Gly Pro Pro Thr Arg Leu 7380 7385 7390 Glu Pro Ser Asp Ile Thr Lys Asp Ala Val Thr Leu Thr Trp Cys Glu 7395 7400 7405 Pro Asp Asp Asp Gly Gly Ser Pro Ile Thr Gly Tyr Trp Val Glu Arg 7410 7415 7420 Leu Asp Pro Asp Thr Asp Lys Trp Val Arg Cys Asn Lys Met Pro Val 7425 7430 7435 7440 Lys Asp Thr Thr Tyr Arg Val Lys Gly Leu Thr Asn Lys Lys Lys Tyr 7445 7450 7455 Arg Phe Arg Val Leu Ala Glu Asn Leu Ala Gly Pro Gly Lys Pro Ser 7460 7465 7470 Lys Ser Thr Glu Pro Ile Leu Ile Lys Asp Pro Ile Asp Pro Pro Trp 7475 7480 7485 Pro Pro Gly Lys Pro Thr Val Lys Asp Val Gly Lys Thr Ser Val Arg 7490 7495 7500 Leu Asn Trp Thr Lys Pro Glu His Asp Gly Gly Ala Lys Ile Glu Ser 7505 7510 7515 7520 Tyr Val Ile Glu Met Leu Lys Thr Gly Thr Asp Glu Trp Val Arg Val 7525 7530 7535 Ala Glu Gly Val Pro Thr Thr Gln His Leu Leu Pro Gly Leu Met Glu 7540 7545 7550 Gly Gln Glu Tyr Ser Phe Arg Val Arg Ala Val Asn Lys Ala Gly Glu 7555 7560 7565 Ser Glu Pro Ser Glu Pro Ser Asp Pro Val Leu Cys Arg Glu Lys Leu 7570 7575 7580 Tyr Pro Pro Ser Pro Pro Arg Trp Leu Glu Val Ile Asn Ile Thr Lys 7585 7590 7595 7600 Asn Thr Ala Asp Leu Lys Trp Thr Val Pro Glu Lys Asp Gly Gly Ser 7605 7610 7615 Pro Ile Thr Asn Tyr Ile Val Glu Lys Arg Asp Val Arg Arg Lys Gly 7620 7625 7630 Trp Gln Thr Val Asp Thr Thr Val Lys Asp Thr Lys Cys Thr Val Thr 7635 7640 7645 Pro Leu Thr Glu Gly Ser Leu Tyr Val Phe Arg Val Ala Ala Glu Asn 7650 7655 7660 Ala Ile Gly Gln Ser Asp Tyr Thr Glu Ile Glu Asp Ser Val Leu Ala 7665 7670 7675 7680 Lys Asp Thr Phe Thr Thr Pro Gly Pro Pro Tyr Ala Leu Ala Val Val 7685 7690 7695 Asp Val Thr Lys Arg His Val Asp Leu Lys Trp Glu Pro Pro Lys Asn 7700 7705 7710 Asp Gly Gly Arg Pro Ile Gln Arg Tyr Val Ile Glu Lys Lys Glu Arg 7715 7720 7725 Leu Gly Thr Arg Trp Val Lys Ala Gly Lys Thr Ala Gly Pro Asp Cys 7730 7735 7740 Asn Phe Arg Val Thr Asp Val Ile Glu Gly Thr Glu Val Gln Phe Gln 7745 7750 7755 7760 Val Arg Ala Glu Asn Glu Ala Gly Val Gly His Pro Ser Glu Pro Thr 7765 7770 7775 Glu Ile Leu Ser Ile Glu Asp Pro Thr Ser Pro Pro Ser Pro Pro Leu 7780 7785 7790 Asp Leu His Val Thr Asp Ala Gly Arg Lys His Ile Ala Ile Ala Trp 7795 7800 7805 Lys Pro Pro Glu Lys Asn Gly Gly Ser Pro Ile Ile Gly Tyr His Val 7810 7815 7820 Glu Met Cys Pro Val Gly Thr Glu Lys Trp Met Arg Val Asn Ser Arg 7825 7830 7835 7840 Pro Ile Lys Asp Leu Lys Phe Lys Val Glu Glu Gly Val Val Pro Asp 7845 7850 7855 Lys Glu Tyr Val Leu Arg Val Arg Ala Val Asn Ala Ile Gly Val Ser 7860 7865 7870 Glu Pro Ser Glu Ile Ser Glu Asn Val Val Ala Lys Asp Pro Asp Cys 7875 7880 7885 Lys Pro Thr Ile Asp Leu Glu Thr His Asp Ile Ile Val Ile Glu Gly 7890 7895 7900 Glu Lys Leu Ser Ile Pro Val Pro Phe Arg Ala Val Pro Val Pro Thr 7905 7910 7915 7920 Val Ser Trp His Lys Asp Gly Lys Glu Val Lys Ala Ser Asp Arg Leu 7925 7930 7935 Thr Met Lys Asn Asp His Ile Ser Ala His Leu Glu Val Pro Lys Ser 7940 7945 7950 Val Arg Ala Asp Ala Gly Ile Tyr Thr Ile Thr Leu Glu Asn Lys Leu 7955 7960 7965 Gly Ser Ala Thr Ala Ser Ile Asn Val Lys Val Ile Gly Leu Pro Gly 7970 7975 7980 Pro Cys Lys Asp Ile Lys Ala Ser Asp Ile Thr Lys Ser Ser Cys Lys 7985 7990 7995 8000 Leu Thr Trp Glu Pro Pro Glu Phe Asp Gly Gly Thr Pro Ile Leu His 8005 8010 8015 Tyr Val Leu Glu Arg Arg Glu Ala Gly Arg Arg Thr Tyr Ile Pro Val 8020 8025 8030 Met Ser Gly Glu Asn Lys Leu Ser Trp Thr Val Lys Asp Leu Ile Pro 8035 8040 8045 Asn Gly Glu Tyr Phe Phe Arg Val Lys Ala Val Asn Lys Val Gly Gly 8050 8055 8060 Gly Glu Tyr Ile Glu Leu Lys Asn Pro Val Ile Ala Gln Asp Pro Lys 8065 8070 8075 8080 Gln Pro Pro Asp Pro Pro Val Asp Val Glu Val His Asn Pro Thr Ala 8085 8090 8095 Glu Ala Met Thr Ile Thr Trp Lys Pro Pro Leu Tyr Asp Gly Gly Ser 8100 8105 8110 Lys Ile Met Gly Tyr Ile Ile Glu Lys Ile Ala Lys Gly Glu Glu Arg 8115 8120 8125 Trp Lys Arg Cys Asn Glu His Leu Val Pro Ile Leu Thr Tyr Thr Ala 8130 8135 8140 Lys Gly Leu Glu Glu Gly Lys Glu Tyr Gln Phe Arg Val Arg Ala Glu 8145 8150 8155 8160 Asn Ala Ala Gly Ile Ser Glu Pro Ser Arg Ala Thr Pro Pro Thr Lys 8165 8170 8175 Ala Val Asp Pro Ile Asp Ala Pro Lys Val Ile Leu Arg Thr Ser Leu 8180 8185 8190 Glu Val Lys Arg Gly Asp Glu Ile Ala Leu Asp Ala Ser Ile Ser Gly 8195 8200 8205 Ser Pro Tyr Pro Thr Ile Thr Trp Ile Lys Asp Glu Asn Val Ile Val 8210 8215 8220 Pro Glu Glu Ile Lys Lys Arg Ala Ala Pro Leu Val Arg Arg Arg Lys 8225 8230 8235 8240 Gly Glu Val Gln Glu Glu Glu Pro Phe Val Leu Pro Leu Thr Gln Arg 8245 8250 8255 Leu Ser Ile Asp Asn Ser Lys Lys Gly Glu Ser Gln Leu Arg Val Arg 8260 8265 8270 Asp Ser Leu Arg Pro Asp His Gly Leu Tyr Met Ile Lys Val Glu Asn 8275 8280 8285 Asp His Gly Ile Ala Lys Ala Pro Cys Thr Val Ser Val Leu Asp Thr 8290 8295 8300 Pro Gly Pro Pro Ile Asn Phe Val Phe Glu Asp Ile Arg Lys Thr Ser 8305 8310 8315 8320 Val Leu Cys Lys Trp Glu Pro Pro Leu Asp Asp Gly Gly Ser Glu Ile 8325 8330 8335 Ile Asn Tyr Thr Leu Glu Lys Lys Asp Lys Thr Lys Pro Asp Ser Glu 8340 8345 8350 Trp Ile Val Val Thr Ser Thr Leu Arg His Cys Lys Tyr Ser Val Thr 8355 8360 8365 Lys Leu Ile Glu Gly Lys Glu Tyr Leu Phe Arg Val Arg Ala Glu Asn 8370 8375 8380 Arg Phe Gly Pro Gly Pro Pro Cys Val Ser Lys Pro Leu Val Ala Lys 8385 8390 8395 8400 Asp Pro Phe Gly Pro Pro Asp Ala Pro Asp Lys Pro Ile Val Glu Asp 8405 8410 8415 Val Thr Ser Asn Ser Met Leu Val Lys Trp Asn Glu Pro Lys Asp Asn 8420 8425 8430 Gly Ser Pro Ile Leu Gly Tyr Trp Leu Glu Lys Arg Glu Val Asn Ser 8435 8440 8445 Thr His Trp Ser Arg Val Asn Lys Ser Leu Leu Asn Ala Leu Lys Ala 8450 8455 8460 Asn Val Asp Gly Leu Leu Glu Gly Leu Thr Tyr Val Phe Arg Val Cys 8465 8470 8475 8480 Ala Glu Asn Ala Ala Gly Pro Gly Lys Phe Ser Pro Pro Ser Asp Pro 8485 8490 8495 Lys Thr Ala His Asp Pro Ile Ser Pro Pro Gly Pro Pro Ile Pro Arg 8500 8505 8510 Val Thr Asp Thr Ser Ser Thr Thr Ile Glu Leu Glu Trp Glu Pro Pro 8515 8520 8525 Ala Phe Asn Gly Gly Gly Glu Ile Val Gly Tyr Phe Val Asp Lys Gln 8530 8535 8540 Leu Val Gly Thr Asn Lys Trp Ser Arg Cys Thr Glu Lys Met Ile Lys 8545 8550 8555 8560 Val Arg Gln Tyr Thr Val Lys Glu Ile Arg Glu Gly Ala Asp Tyr Lys 8565 8570 8575 Leu Arg Val Ser Ala Val Asn Ala Ala Gly Glu Gly Pro Pro Gly Glu 8580 8585 8590 Thr Gln Pro Val Thr Val Ala Glu Pro Gln Glu Pro Pro Ala Val Glu 8595 8600 8605 Leu Asp Val Ser Val Lys Gly Gly Ile Gln Ile Met Ala Gly Lys Thr 8610 8615 8620 Leu Arg Ile Pro Ala Val Val Thr Gly Arg Pro Val Pro Thr Lys Val 8625 8630 8635 8640 Trp Thr Lys Glu Glu Gly Glu Leu Asp Lys Asp Arg Val Val Ile Asp 8645 8650 8655 Asn Val Gly Thr Lys Ser Glu Leu Ile Ile Lys Asp Ala Leu Arg Lys 8660 8665 8670 Asp His Gly Arg Tyr Val Ile Thr Ala Thr Asn Ser Cys Gly Ser Lys 8675 8680 8685 Phe Ala Ala Ala Arg Val Glu Val Phe Asp Val Pro Gly Pro Val Leu 8690 8695 8700 Asp Leu Lys Pro Val Val Thr Asn Arg Lys Met Cys Leu Leu Asn Trp 8705 8710 8715 8720 Ser Asp Pro Glu Asp Asp Gly Gly Ser Glu Ile Thr Gly Phe Ile Ile 8725 8730 8735 Glu Arg Lys Asp Ala Lys Met His Thr Trp Arg Gln Pro Ile Glu Thr 8740 8745 8750 Glu Arg Ser Lys Cys Asp Ile Thr Gly Leu Leu Glu Gly Gln Glu Tyr 8755 8760 8765 Lys Phe Arg Val Ile Ala Lys Asn Lys Phe Gly Cys Gly Pro Pro Val 8770 8775 8780 Glu Ile Gly Pro Ile Leu Ala Val Asp Pro Leu Gly Pro Pro Thr Ser 8785 8790 8795 8800 Pro Glu Arg Leu Thr Tyr Thr Glu Arg Gln Arg Ser Thr Ile Thr Leu 8805 8810 8815 Asp Trp Lys Glu Pro Arg Ser Asn Gly Gly Ser Pro Ile Gln Gly Tyr 8820 8825 8830 Ile Ile Glu Lys Arg Arg His Asp Lys Pro Asp Phe Glu Arg Val Asn 8835 8840 8845 Lys Arg Leu Cys Pro Thr Thr Ser Phe Leu Val Glu Asn Leu Asp Glu 8850 8855 8860 His Gln Met Tyr Glu Phe Arg Val Lys Ala Val Asn Glu Ile Gly Glu 8865 8870 8875 8880 Ser Glu Pro Ser Leu Pro Leu Asn Val Val Ile Gln Asp Asp Glu Val 8885 8890 8895 Pro Pro Thr Ile Lys Leu Arg Leu Ser Val Arg Gly Asp Thr Ile Lys 8900 8905 8910 Val Lys Ala Gly Glu Pro Val His Ile Pro Ala Asp Val Thr Gly Leu 8915 8920 8925 Pro Met Pro Lys Ile Glu Trp Ser Lys Asn Glu Thr Val Ile Glu Lys 8930 8935 8940 Pro Thr Asp Ala Leu Gln Ile Thr Lys Glu Glu Val Ser Arg Ser Glu 8945 8950 8955 8960 Ala Lys Thr Glu Leu Ser Ile Pro Lys Ala Val Arg Glu Asp Lys Gly 8965 8970 8975 Thr Tyr Thr Val Thr Ala Ser Asn Arg Leu Gly Ser Val Phe Arg Asn 8980 8985 8990 Val His Val Glu Val Tyr Asp Arg Pro Ser Pro Pro Arg Asn Leu Ala 8995 9000 9005 Val Thr Asp Ile Lys Ala Glu Ser Cys Tyr Leu Thr Trp Asp Ala Pro 9010 9015 9020 Leu Asp Asn Gly Gly Ser Glu Ile Thr His Tyr Val Ile Asp Lys Arg 9025 9030 9035 9040 Asp Ala Ser Arg Lys Lys Ala Glu Trp Glu Glu Val Thr Asn Thr Ala 9045 9050 9055 Val Glu Lys Arg Tyr Gly Ile Trp Lys Leu Ile Pro Asn Gly Gln Tyr 9060 9065 9070 Glu Phe Arg Val Arg Ala Val Asn Lys Tyr Gly Ile Ser Asp Glu Cys 9075 9080 9085 Lys Ser Asp Lys Val Val Ile Gln Asp Pro Tyr Arg Leu Pro Gly Pro 9090 9095 9100 Pro Gly Lys Pro Lys Val Leu Ala Arg Thr Lys Gly Ser Met Leu Val 9105 9110 9115 9120 Ser Trp Thr Pro Pro Leu Asp Asn Gly Gly Ser Pro Ile Thr Gly Tyr 9125 9130 9135 Trp Leu Glu Lys Arg Glu Glu Gly Ser Pro Tyr Trp Ser Arg Val Ser 9140 9145 9150 Arg Ala Pro Ile Thr Lys Val Gly Leu Lys Gly Val Glu Phe Asn Val 9155 9160 9165 Pro Arg Leu Leu Glu Gly Val Lys Tyr Gln Phe Arg Ala Met Ala Ile 9170 9175 9180 Asn Ala Ala Gly Ile Gly Pro Pro Ser Glu Pro Ser Asp Pro Glu Val 9185 9190 9195 9200 Ala Gly Asp Pro Ile Phe Pro Pro Gly Pro Pro Ser Cys Pro Glu Val 9205 9210 9215 Lys Asp Lys Thr Lys Ser Ser Ile Ser Leu Gly Trp Lys Pro Pro Ala 9220 9225 9230 Lys Asp Gly Gly Ser Pro Ile Lys Gly Tyr Ile Val Glu Met Gln Glu 9235 9240 9245 Glu Gly Thr Thr Asp Trp Lys Arg Val Asn Glu Pro Asp Lys Leu Ile 9250 9255 9260 Thr Thr Cys Glu Cys Val Val Pro Asn Leu Lys Glu Leu Arg Lys Tyr 9265 9270 9275 9280 Arg Phe Arg Val Lys Ala Val Asn Glu Ala Gly Glu Ser Glu Pro Ser 9285 9290 9295 Asp Thr Thr Gly Glu Ile Pro Ala Thr Asp Ile Gln Glu Glu Pro Glu 9300 9305 9310 Val Phe Ile Asp Ile Gly Ala Gln Asp Cys Leu Val Cys Lys Ala Gly 9315 9320 9325 Ser Gln Ile Arg Ile Pro Ala Val Ile Lys Gly Arg Pro Thr Pro Lys 9330 9335 9340 Ser Ser Trp Glu Phe Asp Gly Lys Ala Lys Lys Ala Met Lys Asp Gly 9345 9350 9355 9360 Val His Asp Ile Pro Glu Asp Ala Gln Leu Glu Thr Ala Glu Asn Ser 9365 9370 9375 Ser Val Ile Ile Ile Pro Glu Cys Lys Arg Ser His Thr Gly Lys Tyr 9380 9385 9390 Ser Ile Thr Ala Lys Asn Lys Ala Gly Gln Lys Thr Ala Asn Cys Arg 9395 9400 9405 Val Lys Val Met Asp Val Pro Gly Pro Pro Lys Asp Leu Lys Val Ser 9410 9415 9420 Asp Ile Thr Arg Gly Ser Cys Arg Leu Ser Trp Lys Met Pro Asp Asp 9425 9430 9435 9440 Asp Gly Gly Asp Arg Ile Lys Gly Tyr Val Ile Glu Lys Arg Thr Ile 9445 9450 9455 Asp Gly Lys Ala Trp Thr Lys Val Asn Pro Asp Cys Gly Ser Thr Thr 9460 9465 9470 Phe Val Val Pro Asp Leu Leu Ser Glu Gln Gln Tyr Phe Phe Arg Val 9475 9480 9485 Arg Ala Glu Asn Arg Phe Gly Ile Gly Pro Pro Val Glu Thr Ile Gln 9490 9495 9500 Arg Thr Thr Ala Arg Asp Pro Ile Tyr Pro Pro Asp Pro Pro Ile Lys 9505 9510 9515 9520 Leu Lys Ile Gly Leu Ile Thr Lys Asn Thr Val His Leu Ser Trp Lys 9525 9530 9535 Pro Pro Lys Asn Asp Gly Gly Ser Pro Val Thr His Tyr Ile Val Glu 9540 9545 9550 Cys Leu Ala Trp Asp Pro Thr Gly Thr Lys Lys Glu Ala Trp Arg Gln 9555 9560 9565 Cys Asn Lys Arg Asp Val Glu Glu Leu Gln Phe Thr Val Glu Asp Leu 9570 9575 9580 Val Glu Gly Gly Glu Tyr Glu Phe Arg Val Lys Ala Val Asn Ala Ala 9585 9590 9595 9600 Gly Val Ser Lys Pro Ser Ala Thr Val Gly Pro Cys Asp Cys Gln Arg 9605 9610 9615 Pro Asp Met Pro Pro Ser Ile Asp Leu Lys Glu Phe Met Glu Val Glu 9620 9625 9630 Glu Gly Thr Asn Val Asn Ile Val Ala Lys Ile Lys Gly Val Pro Phe 9635 9640 9645 Pro Thr Leu Thr Trp Phe Lys Ala Pro Pro Lys Lys Pro Asp Asn Lys 9650 9655 9660 Glu Pro Val Leu Tyr Asp Thr His Val Asn Lys Leu Val Val Asp Asp 9665 9670 9675 9680 Thr Cys Thr Leu Val Ile Pro Gln Ser Arg Arg Ser Asp Thr Gly Leu 9685 9690 9695 Tyr Thr Ile Thr Ala Val Asn Asn Leu Gly Thr Ala Ser Lys Glu Met 9700 9705 9710 Arg Leu Asn Val Leu Gly Arg Pro Gly Pro Pro Val Gly Pro Ile Lys 9715 9720 9725 Phe Glu Ser Val Ser Ala Asp Gln Met Thr Leu Ser Trp Phe Pro Pro 9730 9735 9740 Lys Asp Asp Gly Gly Ser Lys Ile Thr Asn Tyr Val Ile Glu Lys Arg 9745 9750 9755 9760 Glu Ala Asn Arg Lys Thr Trp Val His Val Ser Ser Glu Pro Lys Glu 9765 9770 9775 Cys Thr Tyr Thr Ile Pro Lys Leu Leu Glu Gly His Glu Tyr Val Phe 9780 9785 9790 Arg Ile Met Ala Gln Asn Lys Tyr Gly Ile Gly Glu Pro Leu Asp Ser 9795 9800 9805 Glu Pro Glu Thr Ala Arg Asn Leu Phe Ser Val Pro Gly Ala Pro Asp 9810 9815 9820 Lys Pro Thr Val Ser Ser Val Thr Arg Asn Ser Met Thr Val Asn Trp 9825 9830 9835 9840 Glu Glu Pro Glu Tyr Asp Gly Gly Ser Pro Val Thr Gly Tyr Trp Leu 9845 9850 9855 Glu Met Lys Asp Thr Thr Ser Lys Arg Trp Lys Arg Val Asn Arg Asp 9860 9865 9870 Pro Ile Lys Ala Met Thr Leu Gly Val Ser Tyr Lys Val Thr Gly Leu 9875 9880 9885 Ile Glu Gly Ser Asp Tyr Gln Phe Arg Val Tyr Ala Ile Asn Ala Ala 9890 9895 9900 Gly Val Gly Pro Ala Ser Leu Pro Ser Asp Pro Ala Thr Ala Arg Asp 9905 9910 9915 9920 Pro Ile Ala Pro Pro Gly Pro Pro Phe Pro Lys Val Thr Asp Trp Thr 9925 9930 9935 Lys Ser Ser Ala Asp Leu Glu Trp Ser Pro Pro Leu Lys Asp Gly Gly 9940 9945 9950 Ser Lys Val Thr Gly Tyr Ile Val Glu Tyr Lys Glu Glu Gly Lys Glu 9955 9960 9965 Glu Trp Glu Lys Gly Lys Asp Lys Glu Val Arg Gly Thr Lys Leu Val 9970 9975 9980 Val Thr Gly Leu Lys Glu Gly Ala Phe Tyr Lys Phe Arg Val Ser 9985 9990 9995 Ala Val Asn Ile Ala Gly Ile Gly Glu Pro Gly Glu Val Thr Asp 10000 10005 10010 Val Ile Glu Met Lys Asp Arg Leu Val Ser Pro Asp Leu Gln Leu 10015 10020 10025 Asp Ala Ser Val Arg Asp Arg Ile Val Val His Ala Gly Gly Val 10030 10035 10040 Ile Arg Ile Ile Ala Tyr Val Ser Gly Lys Pro Pro Pro Thr Val 10045 10050 10055 Thr Trp Asn Met Asn Glu Arg Thr Leu Pro Gln Glu Ala Thr Ile 10060 10065 10070 Glu Thr Thr Ala Ile Ser Ser Ser Met Val Ile Lys Asn Cys Gln 10075 10080 10085 Arg Ser His Gln Gly Val Tyr Ser Leu Leu Ala Lys Asn Glu Ala 10090 10095 10100 Gly Glu Arg Lys Lys Thr Ile Ile Val Asp Val Leu Asp Val Pro 10105 10110 10115 Gly Pro Val Gly Thr Pro Phe Leu Ala His Asn Leu Thr Asn Glu 10120 10125 10130 Ser Cys Lys Leu Thr Trp Phe Ser Pro Glu Asp Asp Gly Gly Ser 10135 10140 10145 Pro Ile Thr Asn Tyr Val Ile Glu Lys Arg Glu Ser Asp Arg Arg 10150 10155 10160 Ala Trp Thr Pro Val Thr Tyr Thr Val Thr Arg Gln Asn Ala Thr 10165 10170 10175 Val Gln Gly Leu Ile Gln Gly Lys Ala Tyr Phe Phe Arg Ile Ala 10180 10185 10190 Ala Glu Asn Ser Ile Gly Met Gly Pro Phe Val Glu Thr Ser Glu 10195 10200 10205 Ala Leu Val Ile Arg Glu Pro Ile Thr Val Pro Glu Arg Pro Glu 10210 10215 10220 Asp Leu Glu Val Lys Glu Val Thr Lys Asn Thr Val Thr Leu Thr 10225 10230 10235 Trp Asn Pro Pro Lys Tyr Asp Gly Gly Ser Glu Ile Ile Asn Tyr 10240 10245 10250 Val Leu Glu Ser Arg Leu Ile Gly Thr Glu Lys Phe His Lys Val 10255 10260 10265 Thr Asn Asp Asn Leu Leu Ser Arg Lys Tyr Thr Val Lys Gly Leu 10270 10275 10280 Lys Glu Gly Asp Thr Tyr Glu Tyr Arg Val Ser Ala Val Asn Ile 10285 10290 10295 Val Gly Gln Gly Lys Pro Ser Phe Cys Thr Lys Pro Ile Thr Cys 10300 10305 10310 Lys Asp Glu Leu Ala Pro Pro Thr Leu His Leu Asp Phe Arg Asp 10315 10320 10325 Lys Leu Thr Ile Arg Val Gly Glu Ala Phe Ala Leu Thr Gly Arg 10330 10335 10340 Tyr Ser Gly Lys Pro Lys Pro Lys Val Ser Trp Phe Lys Asp Glu 10345 10350 10355 Ala Asp Val Leu Glu Asp Asp Arg Thr His Ile Lys Thr Thr Pro 10360 10365 10370 Ala Thr Leu Ala Leu Glu Lys Ile Lys Ala Lys Arg Ser Asp Ser 10375 10380 10385 Gly Lys Tyr Cys Val Val Val Glu Asn Ser Thr Gly Ser Arg Lys 10390 10395 10400 Gly Phe Cys Gln Val Asn Val Val Asp His Pro Gly Pro Pro Val 10405 10410 10415 Gly Pro Val Ser Phe Asp Glu Val Thr Lys Asp Tyr Met Val Ile 10420 10425 10430 Ser Trp Lys Pro Pro Leu Asp Asp Gly Gly Ser Lys Ile Thr Asn 10435 10440 10445 Tyr Ile Ile Glu Lys Lys Glu Val Gly Lys Asp Val Trp Met Pro 10450 10455 10460 Val Thr Ser Ala Ser Ala Lys Thr Thr Cys Lys Val Ser Lys Leu 10465 10470 10475 Leu Glu Gly Lys Asp Tyr Ile Phe Arg Ile His Ala Glu Asn Leu 10480 10485 10490 Tyr Gly Ile Ser Asp Pro Leu Val Ser Asp Ser Met Lys Ala Lys 10495 10500 10505 Asp Arg Phe Arg Val Pro Asp Ala Pro Asp Gln Pro Ile Val Thr 10510 10515 10520 Glu Val Thr Lys Asp Ser Ala Leu Val Thr Trp Asn Lys Pro His 10525 10530 10535 Asp Gly Gly Lys Pro Ile Thr Asn Tyr Ile Leu Glu Lys Arg Glu 10540 10545 10550 Thr Met Ser Lys Arg Trp Ala Arg Val Thr Lys Asp Pro Ile His 10555 10560 10565 Pro Tyr Thr Lys Phe Arg Val Pro Asp Leu Leu Glu Gly Cys Gln 10570 10575 10580 Tyr Glu Phe Arg Val Ser Ala Glu Asn Glu Ile Gly Ile Gly Asp 10585 10590 10595 Pro Ser Pro Pro Ser Lys Pro Val Phe Ala Lys Asp Pro Ile Ala 10600 10605 10610 Lys Pro Ser Pro Pro Val Asn Pro Glu Ala Ile Asp Thr Thr Cys 10615 10620 10625 Asn Ser Val Asp Leu Thr Trp Gln Pro Pro Arg His Asp Gly Gly 10630 10635 10640 Ser Lys Ile Leu Gly Tyr Ile Val Glu Tyr Gln Lys Val Gly Asp 10645 10650 10655 Glu Glu Trp Arg Arg Ala Asn His Thr Pro Glu Ser Cys Pro Glu 10660 10665 10670 Thr Lys Tyr Lys Val Thr Gly Leu Arg Asp Gly Gln Thr Tyr Lys 10675 10680 10685 Phe Arg Val Leu Ala Val Asn Ala Ala Gly Glu Ser Asp Pro Ala 10690 10695 10700 His Val Pro Glu Pro Val Leu Val Lys Asp Arg Leu Glu Pro Pro 10705 10710 10715 Glu Leu Ile Leu Asp Ala Asn Met Ala Arg Glu Gln His Ile Lys 10720 10725 10730 Val Gly Asp Thr Leu Arg Leu Ser Ala Ile Ile Lys Gly Val Pro 10735 10740 10745 Phe Pro Lys Val Thr Trp Lys Lys Glu Asp Arg Asp Ala Pro Thr 10750 10755 10760 Lys Ala Arg Ile Asp Val Thr Pro Val Gly Ser Lys Leu Glu Ile 10765 10770 10775 Arg Asn Ala Ala His Glu Asp Gly Gly Ile Tyr Ser Leu Thr Val 10780 10785 10790 Glu Asn Pro Ala Gly Ser Lys Thr Val Ser Val Lys Val Leu Val 10795 10800 10805 Leu Asp Lys Pro Gly Pro Pro Arg Asp Leu Glu Val Ser Glu Ile 10810 10815 10820 Arg Lys Asp Ser Cys Tyr Leu Thr Trp Lys Glu Pro Leu Asp Asp 10825 10830 10835 Gly Gly Ser Val Ile Thr Asn Tyr Val Val Glu Arg Arg Asp Val 10840 10845 10850 Ala Ser Ala Gln Trp Ser Pro Leu Ser Ala Thr Ser Lys Lys Lys 10855 10860 10865 Ser His Phe Ala Lys His Leu Asn Glu Gly Asn Gln Tyr Leu Phe 10870 10875 10880 Arg Val Ala Ala Glu Asn Gln Tyr Gly Arg Gly Pro Phe Val Glu 10885 10890 10895 Thr Pro Lys Pro Ile Lys Ala Leu Asp Pro Leu His Pro Pro Gly 10900 10905 10910 Pro Pro Lys Asp Leu His His Val Asp Val Asp Lys Thr Glu Val 10915 10920 10925 Ser Leu Val Trp Asn Lys Pro Asp Arg Asp Gly Gly Ser Pro Ile 10930 10935 10940 Thr Gly Tyr Leu Val Glu Tyr Gln Glu Glu Gly Thr Gln Asp Trp 10945 10950 10955 Ile Lys Phe Lys Thr Val Thr Asn Leu Glu Cys Val Val Thr Gly 10960 10965 10970 Leu Gln Gln Gly Lys Thr Tyr Arg Phe Arg Val Lys Ala Glu Asn 10975 10980 10985 Ile Val Gly Leu Gly Leu Pro Asp Thr Thr Ile Pro Ile Glu Cys 10990 10995 11000 Gln Glu Lys Leu Val Pro Pro Ser Val Glu Leu Asp Val Lys Leu 11005 11010 11015 Ile Glu Gly Leu Val Val Lys Ala Gly Thr Thr Val Arg Phe Pro 11020 11025 11030 Ala Ile Ile Arg Gly Val Pro Val Pro Thr Ala Lys Trp Thr Thr 11035 11040 11045 Asp Gly Ser Glu Ile Lys Thr Asp Glu His Tyr Thr Val Glu Thr 11050 11055 11060 Asp Asn Phe Ser Ser Val Leu Thr Ile Lys Asn Cys Leu Arg Arg 11065 11070 11075 Asp Thr Gly Glu Tyr Gln Ile Thr Val Ser Asn Ala Ala Gly Ser 11080 11085 11090 Lys Thr Val Ala Val His Leu Thr Val Leu Asp Val Pro Gly Pro 11095 11100 11105 Pro Thr Gly Pro Ile Asn Ile Leu Asp Val Thr Pro Glu His Met 11110 11115 11120 Thr Ile Ser Trp Gln Pro Pro Lys Asp Asp Gly Gly Ser Pro Val 11125 11130 11135 Ile Asn Tyr Ile Val Glu Lys Gln Asp Thr Arg Lys Asp Thr Trp 11140 11145 11150 Gly Val Val Ser Ser Gly Ser Ser Lys Thr Lys Leu Lys Ile Pro 11155 11160 11165 His Leu Gln Lys Gly Cys Glu Tyr Val Phe Arg Val Arg Ala Glu 11170 11175 11180 Asn Lys Ile Gly Val Gly Pro Pro Leu Asp Ser Thr Pro Thr Val 11185 11190 11195 Ala Lys His Lys Phe Ser Pro Pro Ser Pro Pro Gly Lys Pro Val 11200 11205 11210 Val Thr Asp Ile Thr Glu Asn Ala Ala Thr Val Ser Trp Thr Leu 11215 11220 11225 Pro Lys Ser Asp Gly Gly Ser Pro Ile Thr Gly Tyr Tyr Met Glu 11230 11235 11240 Arg Arg Glu Val Thr Gly Lys Trp Val Arg Val Asn Lys Thr Pro 11245 11250 11255 Ile Ala Asp Leu Lys Phe Arg Val Thr Gly Leu Tyr Glu Gly Asn 11260 11265 11270 Thr Tyr Glu Phe Arg Val Phe Ala Glu Asn Leu Ala Gly Leu Ser 11275 11280 11285 Lys Pro Ser Pro Ser Ser Asp Pro Ile Lys Ala Cys Arg Pro Ile 11290 11295 11300 Lys Pro Pro Gly Pro Pro Ile Asn Pro Lys Leu Lys Asp Lys Ser 11305 11310 11315 Arg Glu Thr Ala Asp Leu Val Trp Thr Lys Pro Leu Ser Asp Gly 11320 11325 11330 Gly Ser Pro Ile Leu Gly Tyr Val Val Glu Cys Gln Lys Pro Gly 11335 11340 11345 Thr Ala Gln Trp Asn Arg Ile Asn Lys Asp Glu Leu Ile Arg Gln 11350 11355 11360 Cys Ala Phe Arg Val Pro Gly Leu Ile Glu Gly Asn Glu Tyr Arg 11365 11370 11375 Phe Arg Ile Lys Ala Ala Asn Ile Val Gly Glu Gly Glu Pro Arg 11380 11385 11390 Glu Leu Ala Glu Ser Val Ile Ala Lys Asp Ile Leu His Pro Pro 11395 11400 11405 Glu Val Glu Leu Asp Val Thr Cys Arg Asp Val Ile Thr Val Arg 11410 11415 11420 Val Gly Gln Thr Ile Arg Ile Leu Ala Arg Val Lys Gly Arg Pro 11425 11430 11435 Glu Pro Asp Ile Thr Trp Thr Lys Glu Gly Lys Val Leu Val Arg 11440 11445 11450 Glu Lys Arg Val Asp Leu Ile Gln Asp Leu Pro Arg Val Glu Leu 11455 11460 11465 Gln Ile Lys Glu Ala Val Arg Ala Asp His Gly Lys Tyr Ile Ile 11470 11475 11480 Ser Ala Lys Asn Ser Ser Gly His Ala Gln Gly Ser Ala Ile Val 11485 11490 11495 Asn Val Leu Asp Arg Pro Gly Pro Cys Gln Asn Leu Lys Val Thr 11500 11505 11510 Asn Val Thr Lys Glu Asn Cys Thr Ile Ser Trp Glu Asn Pro Leu 11515 11520 11525 Asp Asn Gly Gly Ser Glu Ile Thr Asn Phe Ile Val Glu Tyr Arg 11530 11535 11540 Lys Pro Asn Gln Lys Gly Trp Ser Ile Val Ala Ser Asp Val Thr 11545 11550 11555 Lys Arg Leu Ile Lys Ala Asn Leu Leu Ala Asn Asn Glu Tyr Tyr 11560 11565 11570 Phe Arg Val Cys Ala Glu Asn Lys Val Gly Val Gly Pro Thr Ile 11575 11580 11585 Glu Thr Lys Thr Pro Ile Leu Ala Ile Asn Pro Ile Asp Arg Pro 11590 11595 11600 Gly Glu Pro Glu Asn Leu His Ile Ala Asp Lys Gly Lys Thr Phe 11605 11610 11615 Val Tyr Leu Lys Trp Arg Arg Pro Asp Tyr Asp Gly Gly Ser Pro 11620 11625 11630 Asn Leu Ser Tyr His Val Glu Arg Arg Leu Lys Gly Ser Asp Asp 11635 11640 11645 Trp Glu Arg Val His Lys Gly Ser Ile Lys Glu Thr His Tyr Met 11650 11655 11660 Val Asp Arg Cys Val Glu Asn Gln Ile Tyr Glu Phe Arg Val Gln 11665 11670 11675 Thr Lys Asn Glu Gly Gly Glu Ser Asp Trp Val Lys Thr Glu Glu 11680 11685 11690 Val Val Val Lys Glu Asp Leu Gln Lys Pro Val Leu Asp Leu Lys 11695 11700 11705 Leu Ser Gly Val Leu Thr Val Lys Ala Gly Asp Thr Ile Arg Leu 11710 11715 11720 Glu Ala Gly Val Arg Gly Lys Pro Phe Pro Glu Val Ala Trp Thr 11725 11730 11735 Lys Asp Lys Asp Ala Thr Asp Leu Thr Arg Ser Pro Arg Val Lys 11740 11745 11750 Ile Asp Thr Arg Ala Asp Ser Ser Lys Phe Ser Leu Thr Lys Ala 11755 11760 11765 Lys Arg Ser Asp Gly Gly Lys Tyr Val Val Thr Ala Thr Asn Thr 11770 11775 11780 Ala Gly Ser Phe Val Ala Tyr Ala Thr Val Asn Val Leu Asp Lys 11785 11790 11795 Pro Gly Pro Val Arg Asn Leu Lys Ile Val Asp Val Ser Ser Asp 11800 11805 11810 Arg Cys Thr Val Cys Trp Asp Pro Pro Glu Asp Asp Gly Gly Cys 11815 11820 11825 Glu Ile Gln Asn Tyr Ile Leu Glu Lys Cys Glu Thr Lys Arg Met 11830 11835 11840 Val Trp Ser Thr Tyr Ser Ala Thr Val Leu Thr Pro Gly Thr Thr 11845 11850 11855 Val Thr Arg Leu Ile Glu Gly Asn Glu Tyr Ile Phe Arg Val Arg 11860 11865 11870 Ala Glu Asn Lys Ile Gly Thr Gly Pro Pro Thr Glu Ser Lys Pro 11875 11880 11885 Val Ile Ala Lys Thr Lys Tyr Asp Lys Pro Gly Arg Pro Asp Pro 11890 11895 11900 Pro Glu Val Thr Lys Val Ser Lys Glu Glu Met Thr Val Val Trp 11905 11910 11915 Asn Pro Pro Glu Tyr Asp Gly Gly Lys Ser Ile Thr Gly Tyr Phe 11920 11925 11930 Leu Glu Lys Lys Glu Lys His Ser Thr Arg Trp Val Pro Val Asn 11935 11940 11945 Lys Ser Ala Ile Pro Glu Arg Arg Met Lys Val Gln Asn Leu Leu 11950 11955 11960 Pro Asp His Glu Tyr Gln Phe Arg Val Lys Ala Glu Asn Glu Ile 11965 11970 11975 Gly Ile Gly Glu Pro Ser Leu Pro Ser Arg Pro Val Val Ala Lys 11980 11985 11990 Asp Pro Ile Glu Pro Pro Gly Pro Pro Thr Asn Phe Arg Val Val 11995 12000 12005 Asp Thr Thr Lys His Ser Ile Thr Leu Gly Trp Gly Lys Pro Val 12010 12015 12020 Tyr Asp Gly Gly Ala Pro Ile Ile Gly Tyr Val Val Glu Met Arg 12025 12030 12035 Pro Lys Ile Ala Asp Ala Ser Pro Asp Glu Gly Trp Lys Arg Cys 12040 12045 12050 Asn Ala Ala Ala Gln Leu Val Arg Lys Glu Phe Thr Val Thr Ser 12055 12060 12065 Leu Asp Glu Asn Gln Glu Tyr Glu Phe Arg Val Cys Ala Gln Asn 12070 12075 12080 Gln Val Gly Ile Gly Arg Pro Ala Glu Leu Lys Glu Ala Ile Lys 12085 12090 12095 Pro Lys Glu Ile Leu Glu Pro Pro Glu Ile Asp Leu Asp Ala Ser 12100 12105 12110 Met Arg Lys Leu Val Ile Val Arg Ala Gly Cys Pro Ile Arg Leu 12115 12120 12125 Phe Ala Ile Val Arg Gly Arg Pro Ala Pro Lys Val Thr Trp Arg 12130 12135 12140 Lys Val Gly Ile Asp Asn Val Val Arg Lys Gly Gln Val Asp Leu 12145 12150 12155 Val Asp Thr Met Ala Phe Leu Val Ile Pro Asn Ser Thr Arg Asp 12160 12165 12170 Asp Ser Gly Lys Tyr Ser Leu Thr Leu Val Asn Pro Ala Gly Glu 12175 12180 12185 Lys Ala Val Phe Val Asn Val Arg Val Leu Asp Thr Pro Gly Pro 12190 12195 12200 Val Ser Asp Leu Lys Val Ser Asp Val Thr Lys Thr Ser Cys His 12205 12210 12215 Val Ser Trp Ala Pro Pro Glu Asn Asp Gly Gly Ser Gln Val Thr 12220 12225 12230 His Tyr Ile Val Glu Lys Arg Glu Ala Asp Arg Lys Thr Trp Ser 12235 12240 12245 Thr Val Thr Pro Glu Val Lys Lys Thr Ser Phe His Val Thr Asn 12250 12255 12260 Leu Val Pro Gly Asn Glu Tyr Tyr Phe Arg Val Thr Ala Val Asn 12265 12270 12275 Glu Tyr Gly Pro Gly Val Pro Thr Asp Val Pro Lys Pro Val Leu 12280 12285 12290 Ala Ser Asp Pro Leu Ser Glu Pro Asp Pro Pro Arg Lys Leu Glu 12295 12300 12305 Ala Thr Glu Met Thr Lys Asn Ser Ala Thr Leu Ala Trp Leu Pro 12310 12315 12320 Pro Leu Arg Asp Gly Gly Ala Lys Ile Asp Gly Tyr Ile Ile Ser 12325 12330 12335 Tyr Arg Glu Glu Glu Gln Pro Ala Asp Arg Trp Thr Glu Tyr Ser 12340 12345 12350 Val Val Lys Asp Leu Ser Leu Val Val Thr Gly Leu Lys Glu Gly 12355 12360 12365 Lys Lys Tyr Lys Phe Arg Val Ala Ala Arg Asn Ala Val Gly Val 12370 12375 12380 Ser Leu Pro Arg Glu Ala Glu Gly Val Tyr Glu Ala Lys Glu Gln 12385 12390 12395 Leu Leu Pro Pro Lys Ile Leu Met Pro Glu Gln Ile Thr Ile Lys 12400 12405 12410 Ala Gly Lys Lys Leu Arg Ile Glu Ala His Val Tyr Gly Lys Pro 12415 12420 12425 His Pro Thr Cys Lys Trp Lys Lys Gly Glu Asp Glu Val Val Thr 12430 12435 12440 Ser Ser His Leu Ala Val His Lys Ala Asp Ser Ser Ser Ile Leu 12445 12450 12455 Ile Ile Lys Asp Val Thr Arg Lys Asp Ser Gly Tyr Tyr Ser Leu 12460 12465 12470 Thr Ala Glu Asn Ser Ser Gly Thr Asp Thr Gln Lys Ile Lys Val 12475 12480 12485 Val Val Met Asp Ala Pro Gly Pro Pro Gln Pro Pro Phe Asp Ile 12490 12495 12500 Ser Asp Ile Asp Ala Asp Ala Cys Ser Leu Ser Trp His Ile Pro 12505 12510 12515 Leu Glu Asp Gly Gly Ser Asn Ile Thr Asn Tyr Ile Val Glu Lys 12520 12525 12530 Cys Asp Val Ser Arg Gly Asp Trp Val Thr Ala Leu Ala Ser Val 12535 12540 12545 Thr Lys Thr Ser Cys Arg Val Gly Lys Leu Ile Pro Gly Gln Glu 12550 12555 12560 Tyr Ile Phe Arg Val Arg Ala Glu Asn Arg Phe Gly Ile Ser Glu 12565 12570 12575 Pro Leu Thr Ser Pro Lys Met Val Ala Gln Phe Pro Phe Gly Val 12580 12585 12590 Pro Ser Glu Pro Lys Asn Ala Arg Val Thr Lys Val Asn Lys Asp 12595 12600 12605 Cys Ile Phe Val Ala Trp Asp Arg Pro Asp Ser Asp Gly Gly Ser 12610 12615 12620 Pro Ile Ile Gly Tyr Leu Ile Glu Arg Lys Glu Arg Asn Ser Leu 12625 12630 12635 Leu Trp Val Lys Ala Asn Asp Thr Leu Val Arg Ser Thr Glu Tyr 12640 12645 12650 Pro Cys Ala Gly Leu Val Glu Gly Leu Glu Tyr Ser Phe Arg Ile 12655 12660 12665 Tyr Ala Leu Asn Lys Ala Gly Ser Ser Pro Pro Ser Lys Pro Thr 12670 12675 12680 Glu Tyr Val Thr Ala Arg Met Pro Val Asp Pro Pro Gly Lys Pro 12685 12690 12695 Glu Val Ile Asp Val Thr Lys Ser Thr Val Ser Leu Ile Trp Ala 12700 12705 12710 Arg Pro Lys His Asp Gly Gly Ser Lys Ile Ile Gly Tyr Phe Val 12715 12720 12725 Glu Ala Cys Lys Leu Pro Gly Asp Lys Trp Val Arg Cys Asn Thr 12730 12735 12740 Ala Pro His Gln Ile Pro Gln Glu Glu Tyr Thr Ala Thr Gly Leu 12745 12750 12755 Glu Glu Lys Ala Gln Tyr Gln Phe Arg Ala Ile Ala Arg Thr Ala 12760 12765 12770 Val Asn Ile Ser Pro Pro Ser Glu Pro Ser Asp Pro Val Thr Ile 12775 12780 12785 Leu Ala Glu Asn Val Pro Pro Arg Ile Asp Leu Ser Val Ala Met 12790 12795 12800 Lys Ser Leu Leu Thr Val Lys Ala Gly Thr Asn Val Cys Leu Asp 12805 12810 12815 Ala Thr Val Phe Gly Lys Pro Met Pro Thr Val Ser Trp Lys Lys 12820 12825 12830 Asp Gly Thr Leu Leu Lys Pro Ala Glu Gly Ile Lys Met Ala Met 12835 12840 12845 Gln Arg Asn Leu Cys Thr Leu Glu Leu Phe Ser Val Asn Arg Lys 12850 12855 12860 Asp Ser Gly Asp Tyr Thr Ile Thr Ala Glu Asn Ser Ser Gly Ser 12865 12870 12875 Lys Ser Ala Thr Ile Lys Leu Lys Val Leu Asp Lys Pro Gly Pro 12880 12885 12890 Pro Ala Ser Val Lys Ile Asn Lys Met Tyr Ser Asp Arg Ala Met 12895 12900 12905 Leu Ser Trp Glu Pro Pro Leu Glu Asp Gly Gly Ser Glu Ile Thr 12910 12915 12920 Asn Tyr Ile Val Asp Lys Arg Glu Thr Ser Arg Pro Asn Trp Ala 12925 12930 12935 Gln Val Ser Ala Thr Val Pro Ile Thr Ser Cys Ser Val Glu Lys 12940 12945 12950 Leu Ile Glu Gly His Glu Tyr Gln Phe Arg Ile Cys Ala Glu Asn 12955 12960 12965 Lys Tyr Gly Val Gly Asp Pro Val Phe Thr Glu Pro Ala Ile Ala 12970 12975 12980 Lys Asn Pro Tyr Asp Pro Pro Gly Arg Cys Asp Pro Pro Val Ile 12985 12990 12995 Ser Asn Ile Thr Lys Asp His Met Thr Val Ser Trp Lys Pro Pro 13000 13005 13010 Ala Asp Asp Gly Gly Ser Pro Ile Thr Gly Tyr Leu Leu Glu Lys 13015 13020 13025 Arg Glu Thr Gln Ala Val Asn Trp Thr Lys Val Asn Arg Lys Pro 13030 13035 13040 Ile Ile Glu Arg Thr Leu Lys Ala Thr Gly Leu Gln Glu Gly Thr 13045 13050 13055 Glu Tyr Glu Phe Arg Val Thr Ala Ile Asn Lys Ala Gly Pro Gly 13060 13065 13070 Lys Pro Ser Asp Ala Ser Lys Ala Ala Tyr Ala Arg Asp Pro Gln 13075 13080 13085 Tyr Pro Pro Ala Pro Pro Ala Phe Pro Lys Val Tyr Asp Thr Thr 13090 13095 13100 Arg Ser Ser Val Ser Leu Ser Trp Gly Lys Pro Ala Tyr Asp Gly 13105 13110 13115 Gly Ser Pro Ile Ile Gly Tyr Leu Val Glu Val Lys Arg Ala Asp 13120 13125 13130 Ser Asp Asn Trp Val Arg Cys Asn Leu Pro Gln Asn Leu Gln Lys 13135 13140 13145 Thr Arg Phe Glu Val Thr Gly Leu Met Glu Asp Thr Gln Tyr Gln 13150 13155 13160 Phe Arg Val Tyr Ala Val Asn Lys Ile Gly Tyr Ser Asp Pro Ser 13165 13170 13175 Asp Val Pro Asp Lys His Tyr Pro Lys Asp Ile Leu Ile Pro Pro 13180 13185 13190 Glu Gly Glu His Asp Ala Asp Leu Arg Lys Thr Leu Ile Leu Arg 13195 13200 13205 Ala Gly Val Thr Met Arg Leu Tyr Val Pro Val Lys Gly Arg Pro 13210 13215 13220 Pro Pro Lys Ile Thr Trp Ser Lys Pro Asn Val Asn Leu Arg Asp 13225 13230 13235 Arg Ile Gly Leu Asp Ile Lys Ser Thr Asp Phe Asp Thr Phe Leu 13240 13245 13250 Arg Cys Glu Asn Val Asn Lys Tyr Asp Ala Gly Lys Tyr Ile Leu 13255 13260 13265 Thr Leu Glu Asn Ser Cys Gly Lys Lys Glu Tyr Thr Ile Val Val 13270 13275 13280 Lys Val Leu Asp Thr Pro Gly Pro Pro Ile Asn Val Thr Val Lys 13285 13290 13295 Glu Ile Ser Lys Asp Ser Ala Tyr Val Thr Trp Glu Pro Pro Ile 13300 13305 13310 Ile Asp Gly Gly Ser Pro Ile Ile Asn Tyr Val Val Gln Lys Arg 13315 13320 13325 Asp Ala Glu Arg Lys Ser Trp Ser Thr Val Thr Thr Glu Cys Ser 13330 13335 13340 Lys Thr Ser Phe Arg Val Pro Asn Leu Glu Glu Gly Lys Ser Tyr 13345 13350 13355 Phe Phe Arg Val Phe Ala Glu Asn Glu Tyr Gly Ile Gly Asp Pro 13360 13365 13370 Gly Glu Thr Arg Asp Ala Val Lys Ala Ser Gln Thr Pro Gly Pro 13375 13380 13385 Val Val Asp Leu Lys Val Arg Ser Val Ser Lys Ser Ser Cys Ser 13390 13395 13400 Ile Gly Trp Lys Lys Pro His Ser Asp Gly Gly Ser Arg Ile Ile 13405 13410 13415 Gly Tyr Val Val Asp Phe Leu Thr Glu Glu Asn Lys Trp Gln Arg 13420 13425 13430 Val Met Lys Ser Leu Ser Leu Gln Tyr Ser Ala Lys Asp Leu Thr 13435 13440 13445 Glu Gly Lys Glu Tyr Thr Phe Arg Val Ser Ala Glu Asn Glu Asn 13450 13455 13460 Gly Glu Gly Thr Pro Ser Glu Ile Thr Val Val Ala Arg Asp Asp 13465 13470 13475 Val Val Ala Pro Asp Leu Asp Leu Lys Gly Leu Pro Asp Leu Cys 13480 13485 13490 Tyr Leu Ala Lys Glu Asn Ser Asn Phe Arg Leu Lys Ile Pro Ile 13495 13500 13505 Lys Gly Lys Pro Ala Pro Ser Val Ser Trp Lys Lys Gly Glu Asp 13510 13515 13520 Pro Leu Ala Thr Asp Thr Arg Val Ser Val Glu Ser Ser Ala Val 13525 13530 13535 Asn Thr Thr Leu Ile Val Tyr Asp Cys Gln Lys Ser Asp Ala Gly 13540 13545 13550 Lys Tyr Thr Ile Thr Leu Lys Asn Val Ala Gly Thr Lys Glu Gly 13555 13560 13565 Thr Ile Ser Ile Lys Val Val Gly Lys Pro Gly Ile Pro Thr Gly 13570 13575 13580 Pro Ile Lys Phe Asp Glu Val Thr Ala Glu Ala Met Thr Leu Lys 13585 13590 13595 Trp Ala Pro Pro Lys Asp Asp Gly Gly Ser Glu Ile Thr Asn Tyr 13600 13605 13610 Ile Leu Glu Lys Arg Asp Ser Val Asn Asn Lys Trp Val Thr Cys 13615 13620 13625 Ala Ser Ala Val Gln Lys Thr Thr Phe Arg Val Thr Arg Leu His 13630 13635 13640 Glu Gly Met Glu Tyr Thr Phe Arg Val Ser Ala Glu Asn Lys Tyr 13645 13650 13655 Gly Val Gly Glu Gly Leu Lys Ser Glu Pro Ile Val Ala Arg His 13660 13665 13670 Pro Phe Asp Val Pro Asp Ala Pro Pro Pro Pro Asn Ile Val Asp 13675 13680 13685 Val Arg His Asp Ser Val Ser Leu Thr Trp Thr Asp Pro Lys Lys 13690 13695 13700 Thr Gly Gly Ser Pro Ile Thr Gly Tyr His Leu Glu Phe Lys Glu 13705 13710 13715 Arg Asn Ser Leu Leu Trp Lys Arg Ala Asn Lys Thr Pro Ile Arg 13720 13725 13730 Met Arg Asp Phe Lys Val Thr Gly Leu Thr Glu Gly Leu Glu Tyr 13735 13740 13745 Glu Phe Arg Val Met Ala Ile Asn Leu Ala Gly Val Gly Lys Pro 13750 13755 13760 Ser Leu Pro Ser Glu Pro Val Val Ala Leu Asp Pro Ile Asp Pro 13765 13770 13775 Pro Gly Lys Pro Glu Val Ile Asn Ile Thr Arg Asn Ser Val Thr 13780 13785 13790 Leu Ile Trp Thr Glu Pro Lys Tyr Asp Gly Gly His Lys Leu Thr 13795 13800 13805 Gly Tyr Ile Val Glu Lys Arg Asp Leu Pro Ser Lys Ser Trp Met 13810 13815 13820 Lys Ala Asn His Val Asn Val Pro Glu Cys Ala Phe Thr Val Thr 13825 13830 13835 Asp Leu Val Glu Gly Gly Lys Tyr Glu Phe Arg Ile Arg Ala Lys 13840 13845 13850 Asn Thr Ala Gly Ala Ile Ser Ala Pro Ser Glu Ser Thr Glu Thr 13855 13860 13865 Ile Ile Cys Lys Asp Glu Tyr Glu Ala Pro Thr Ile Val Leu Asp 13870 13875 13880 Pro Thr Ile Lys Asp Gly Leu Thr Ile Lys Ala Gly Asp Thr Ile 13885 13890 13895 Val Leu Asn Ala Ile Ser Ile Leu Gly Lys Pro Leu Pro Lys Ser 13900 13905 13910 Ser Trp Ser Lys Ala Gly Lys Asp Ile Arg Pro Ser Asp Ile Thr 13915 13920 13925 Gln Ile Thr Ser Thr Pro Thr Ser Ser Met Leu Thr Ile Lys Tyr 13930 13935 13940 Ala Thr Arg Lys Asp Ala Gly Glu Tyr Thr Ile Thr Ala Thr Asn 13945 13950 13955 Pro Phe Gly Thr Lys Val Glu His Val Lys Val Thr Val Leu Asp 13960 13965 13970 Val Pro Gly Pro Pro Gly Pro Val Glu Ile Ser Asn Val Ser Ala 13975 13980 13985 Glu Lys Ala Thr Leu Thr Trp Thr Pro Pro Leu Glu Asp Gly Gly 13990 13995 14000 Ser Pro Ile Lys Ser Tyr Ile Leu Glu Lys Arg Glu Thr Ser Arg 14005 14010 14015 Leu Leu Trp Thr Val Val Ser Glu Asp Ile Gln Ser Cys Arg His 14020 14025 14030 Val Ala Thr Lys Leu Ile Gln Gly Asn Glu Tyr Ile Phe Arg Val 14035 14040 14045 Ser Ala Val Asn His Tyr Gly Lys Gly Glu Pro Val Gln Ser Glu 14050 14055 14060 Pro Val Lys Met Val Asp Arg Phe Gly Pro Pro Gly Pro Pro Glu 14065 14070 14075 Lys Pro Glu Val Ser Asn Val Thr Lys Asn Thr Ala Thr Val Ser 14080 14085 14090 Trp Lys Arg Pro Val Asp Asp Gly Gly Ser Glu Ile Thr Gly Tyr 14095 14100 14105 His Val Glu Arg Arg Glu Lys Lys Ser Leu Arg Trp Val Arg Ala 14110 14115 14120 Ile Lys Thr Pro Val Ser Asp Leu Arg Cys Lys Val Thr Gly Leu 14125 14130 14135 Gln Glu Gly Ser Thr Tyr Glu Phe Arg Val Ser Ala Glu Asn Arg 14140 14145 14150 Ala Gly Ile Gly Pro Pro Ser Glu Ala Ser Asp Ser Val Leu Met 14155 14160 14165 Lys Asp Ala Ala Tyr Pro Pro Gly Pro Pro Ser Asn Pro His Val 14170 14175 14180 Thr Asp Thr Thr Lys Lys Ser Ala Ser Leu Ala Trp Gly Lys Pro 14185 14190 14195 His Tyr Asp Gly Gly Leu Glu Ile Thr Gly Tyr Val Val Glu His 14200 14205 14210 Gln Lys Val Gly Asp Glu Ala Trp Ile Lys Asp Thr Thr Gly Thr 14215 14220 14225 Ala Leu Arg Ile Thr Gln Phe Val Val Pro Asp Leu Gln Thr Lys 14230 14235 14240 Glu Lys Tyr Asn Phe Arg Ile Ser Ala Ile Asn Asp Ala Gly Val 14245 14250 14255 Gly Glu Pro Ala Val Ile Pro Asp Val Glu Ile Val Glu Arg Glu 14260 14265 14270 Met Ala Pro Asp Phe Glu Leu Asp Ala Glu Leu Arg Arg Thr Leu 14275 14280 14285 Val Val Arg Ala Gly Leu Ser Ile Arg Ile Phe Val Pro Ile Lys 14290 14295 14300 Gly Arg Pro Ala Pro Glu Val Thr Trp Thr Lys Asp Asn Ile Asn 14305 14310 14315 Leu Lys Asn Arg Ala Asn Ile Glu Asn Thr Glu Ser Phe Thr Leu 14320 14325 14330 Leu Ile Ile Pro Glu Cys Asn Arg Tyr Asp Thr Gly Lys Phe Val 14335 14340 14345 Met Thr Ile Glu Asn Pro Ala Gly Lys Lys Ser Gly Phe Val Asn 14350 14355 14360 Val Arg Val Leu Asp Thr Pro Gly Pro Val Leu Asn Leu Arg Pro 14365 14370 14375 Thr Asp Ile Thr Lys Asp Ser Val Thr Leu His Trp Asp Leu Pro 14380 14385 14390 Leu Ile Asp Gly Gly Ser Arg Ile Thr Asn Tyr Ile Val Glu Lys 14395 14400 14405 Arg Glu Ala Thr Arg Lys Ser Tyr Ser Thr Ala Thr Thr Lys Cys 14410 14415 14420 His Lys Cys Thr Tyr Lys Val Thr Gly Leu Ser Glu Gly Cys Glu 14425 14430 14435 Tyr Phe Phe Arg Val Met Ala Glu Asn Glu Tyr Gly Ile Gly Glu 14440 14445 14450 Pro Thr Glu Thr Thr Glu Pro Val Lys Ala Ser Glu Ala Pro Ser 14455 14460 14465 Pro Pro Asp Ser Leu Asn Ile Met Asp Ile Thr Lys Ser Thr Val 14470 14475 14480 Ser Leu Ala Trp Pro Lys Pro Lys His Asp Gly Gly Ser Lys Ile 14485 14490 14495 Thr Gly Tyr Val Ile Glu Ala Gln Arg Lys Gly Ser Asp Gln Trp 14500 14505 14510 Thr His Ile Thr Thr Val Lys Gly Leu Glu Cys Val Val Arg Asn 14515 14520 14525 Leu Thr Glu Gly Glu Glu Tyr Thr Phe Gln Val Met Ala Val Asn 14530 14535 14540 Ser Ala Gly Arg Ser Ala Pro Arg Glu Ser Arg Pro Val Ile Val 14545 14550 14555 Lys Glu Gln Thr Met Leu Pro Glu Leu Asp Leu Arg Gly Ile Tyr 14560 14565 14570 Gln Lys Leu Val Ile Ala Lys Ala Gly Asp Asn Ile Lys Val Glu 14575 14580 14585 Ile Pro Val Leu Gly Arg Pro Lys Pro Thr Val Thr Trp Lys Lys 14590 14595 14600 Gly Asp Gln Ile Leu Lys Gln Thr Gln Arg Val Asn Phe Glu Thr 14605 14610 14615 Thr Ala Thr Ser Thr Ile Leu Asn Ile Asn Glu Cys Val Arg Ser 14620 14625 14630 Asp Ser Gly Pro Tyr Pro Leu Thr Ala Arg Asn Ile Val Gly Glu 14635 14640 14645 Val Gly Asp Val Ile Thr Ile Gln Val His Asp Ile Pro Gly Pro 14650 14655 14660 Pro Thr Gly Pro Ile Lys Phe Asp Glu Val Ser Ser Asp Phe Val 14665 14670 14675 Thr Phe Ser Trp Asp Pro Pro Glu Asn Asp Gly Gly Val Pro Ile 14680 14685 14690 Ser Asn Tyr Val Val Glu Met Arg Gln Thr Asp Ser Thr Thr Trp 14695 14700 14705 Val Glu Leu Ala Thr Thr Val Ile Arg Thr Thr Tyr Lys Ala Thr 14710 14715 14720 Arg Leu Thr Thr Gly Leu Glu Tyr Gln Phe Arg Val Lys Ala Gln 14725 14730 14735 Asn Arg Tyr Gly Val Gly Pro Gly Ile Thr Ser Ala Trp Ile Val 14740 14745 14750 Ala Asn Tyr Pro Phe Lys Val Pro Gly Pro Pro Gly Thr Pro Gln 14755 14760 14765 Val Thr Ala Val Thr Lys Asp Ser Met Thr Ile Ser Trp His Glu 14770 14775 14780 Pro Leu Ser Asp Gly Gly Ser Pro Ile Leu Gly Tyr His Val Glu 14785 14790 14795 Arg Lys Glu Arg Asn Gly Ile Leu Trp Gln Thr Val Ser Lys Ala 14800 14805 14810 Leu Val Pro Gly Asn Ile Phe Lys Ser Ser Gly Leu Thr Asp Gly 14815 14820 14825 Ile Ala Tyr Glu Phe Arg Val Ile Ala Glu Asn Met Ala Gly Lys 14830 14835 14840 Ser Lys Pro Ser Lys Pro Ser Glu Pro Met Leu Ala Leu Asp Pro 14845 14850 14855 Ile Asp Pro Pro Gly Lys Pro Val Pro Leu Asn Ile Thr Arg His 14860 14865 14870 Thr Val Thr Leu Lys Trp Ala Lys Pro Glu Tyr Thr Gly Gly Phe 14875 14880 14885 Lys Ile Thr Ser Tyr Ile Val Glu Lys Arg Asp Leu Pro Asn Gly 14890 14895 14900 Arg Trp Leu Lys Ala Asn Phe Ser Asn Ile Leu Glu Asn Glu Phe 14905 14910 14915 Thr Val Ser Gly Leu Thr Glu Asp Ala Ala Tyr Glu Phe Arg Val 14920 14925 14930 Ile Ala Lys Asn Ala Ala Gly Ala Ile Ser Pro Pro Ser Glu Pro 14935 14940 14945 Ser Asp Ala Ile Thr Cys Arg Asp Asp Val Glu Ala Pro Lys Ile 14950 14955 14960 Lys Val Asp Val Lys Phe Lys Asp Thr Val Ile Leu Lys Ala Gly 14965 14970 14975 Glu Ala Phe Arg Leu Glu Ala Asp Val Ser Gly Arg Pro Pro Pro 14980 14985 14990 Thr Met Glu Trp Ser Lys Asp Gly Lys Glu Leu Glu Gly Thr Ala 14995 15000 15005 Lys Leu Glu Ile Lys Ile Ala Asp Phe Ser Thr Asn Leu Val Asn 15010 15015 15020 Lys Asp Ser Thr Arg Arg Asp Ser Gly Ala Tyr Thr Leu Thr Ala 15025 15030 15035 Thr Asn Pro Gly Gly Phe Ala Lys His Ile Phe Asn Val Lys Val 15040 15045 15050 Leu Asp Arg Pro Gly Pro Pro Glu Gly Pro Leu Ala Val Thr Glu 15055 15060 15065 Val Thr Ser Glu Lys Cys Val Leu Ser Trp Phe Pro Pro Leu Asp 15070 15075 15080 Asp Gly Gly Ala Lys Ile Asp His Tyr Ile Val Gln Lys Arg Glu 15085 15090 15095 Thr Ser Arg Leu Ala Trp Thr Asn Val Ala Ser Glu Val Gln Val 15100 15105 15110 Thr Lys Leu Lys Val Thr Lys Leu Leu Lys Gly Asn Glu Tyr Ile 15115 15120 15125 Phe Arg Val Met Ala Val Asn Lys Tyr Gly Val Gly Glu Pro Leu 15130 15135 15140 Glu Ser Glu Pro Val Leu Ala Val Asn Pro Tyr Gly Pro Pro Asp 15145 15150 15155 Pro Pro Lys Asn Pro Glu Val Thr Thr Ile Thr Lys Asp Ser Met 15160 15165 15170 Val Val Cys Trp Gly His Pro Asp Ser Asp Gly Gly Ser Glu Ile 15175 15180 15185 Ile Asn Tyr Ile Val Glu Arg Arg Asp Lys Ala Gly Gln Arg Trp 15190 15195 15200 Ile Lys Cys Asn Lys Lys Thr Leu Thr Asp Leu Arg Tyr Lys Val 15205 15210 15215 Ser Gly Leu Thr Glu Gly His Glu Tyr Glu Phe Arg Ile Met Ala 15220 15225 15230 Glu Asn Ala Ala Gly Ile Ser Ala Pro Ser Pro Thr Ser Pro Phe 15235 15240 15245 Tyr Lys Ala Cys Asp Thr Val Phe Lys Pro Gly Pro Pro Gly Asn 15250 15255 15260 Pro Arg Val Leu Asp Thr Ser Arg Ser Ser Ile Ser Ile Ala Trp 15265 15270 15275 Asn Lys Pro Ile Tyr Asp Gly Gly Ser Glu Ile Thr Gly Tyr Met 15280 15285 15290 Val Glu Ile Ala Leu Pro Glu Glu Asp Glu Trp Gln Ile Val Thr 15295 15300 15305 Pro Pro Ala Gly Leu Lys Ala Thr Ser Tyr Thr Ile Thr Gly Leu 15310 15315 15320 Thr Glu Asn Gln Glu Tyr Lys Ile Arg Ile Tyr Ala Met Asn Ser 15325 15330 15335 Glu Gly Leu Gly Glu Pro Ala Leu Val Pro Gly Thr Pro Lys Ala 15340 15345 15350 Glu Asp Arg Met Leu Pro Pro Glu Ile Glu Leu Asp Ala Asp Leu 15355 15360 15365 Arg Lys Val Val Thr Ile Arg Ala Cys Cys Thr Leu Arg Leu Phe 15370 15375 15380 Val Pro Ile Lys Gly Arg Pro Asp Pro Glu Val Lys Trp Ala Arg 15385 15390 15395 Asp His Gly Glu Ser Leu Asp Lys Ala Ser Ile Glu Ser Ala Ser 15400 15405 15410 Ser Tyr Thr Leu Leu Ile Val Gly Asn Val Asn Arg Phe Asp Ser 15415 15420 15425 Gly Lys Tyr Ile Leu Thr Val Glu Asn Ser Ser Gly Ser Lys Ser 15430 15435 15440 Ala Phe Val Asn Val Arg Val Leu Asp Thr Pro Gly Pro Pro Gln 15445 15450 15455 Asp Leu Lys Val Lys Glu Val Thr Lys Thr Ser Val Thr Leu Thr 15460 15465 15470 Trp Asp Pro Pro Leu Leu Asp Gly Gly Ser Lys Ile Lys Asn Tyr 15475 15480 15485 Ile Val Glu Lys Arg Glu Ser Thr Arg Lys Ala Tyr Ser Thr Val 15490 15495 15500 Ala Thr Asn Cys His Lys Thr Ser Trp Lys Val Asp Gln Leu Gln 15505 15510 15515 Glu Gly Cys Ser Tyr Tyr Phe Arg Val Leu Ala Glu Asn Glu Tyr 15520 15525 15530 Gly Ile Gly Leu Pro Ala Glu Thr Ala Glu Ser Val Lys Ala Ser 15535 15540 15545 Glu Arg Pro Leu Pro Pro Gly Lys Ile Thr Leu Met Asp Val Thr 15550 15555 15560 Arg Asn Ser Val Ser Leu Ser Trp Glu Lys Pro Glu His Asp Gly 15565 15570 15575 Gly Ser Arg Ile Leu Gly Tyr Ile Val Glu Met Gln Thr Lys Gly 15580 15585 15590 Ser Asp Lys Trp Ala Thr Cys Ala Thr Val Lys Val Thr Glu Ala 15595 15600 15605 Thr Ile Thr Gly Leu Ile Gln Gly Glu Glu Tyr Ser Phe Arg Val 15610 15615 15620 Ser Ala Gln Asn Glu Lys Gly Ile Ser Asp Pro Arg Gln Leu Ser 15625 15630 15635 Val Pro Val Ile Ala Lys Asp Leu Val Ile Pro Pro Ala Phe Lys 15640 15645 15650 Leu Leu Phe Asn Thr Phe Thr Val Leu Ala Gly Glu Asp Leu Lys 15655 15660 15665 Val Asp Val Pro Phe Ile Gly Arg Pro Thr Pro Ala Val Thr Trp 15670 15675 15680 His Lys Asp Asn Val Pro Leu Lys Gln Thr Thr Arg Val Asn Ala 15685 15690 15695 Glu Ser Thr Glu Asn Asn Ser Leu Leu Thr Ile Lys Asp Ala Cys 15700 15705 15710 Arg Glu Asp Val Gly His Tyr Val Val Lys Leu Thr Asn Ser Ala 15715 15720 15725 Gly Glu Ala Ile Glu Thr Leu Asn Val Ile Val Leu Asp Lys Pro 15730 15735 15740 Gly Pro Pro Thr Gly Pro Val Lys Met Asp Glu Val Thr Ala Asp 15745 15750 15755 Ser Ile Thr Leu Ser Trp Gly Pro Pro Lys Tyr Asp Gly Gly Ser 15760 15765 15770 Ser Ile Asn Asn Tyr Ile Val Glu Lys Arg Asp Thr Ser Thr Thr 15775 15780 15785 Thr Trp Gln Ile Val Ser Ala Thr Val Ala Arg Thr Thr Ile Lys 15790 15795 15800 Ala Cys Arg Leu Lys Thr Gly Cys Glu Tyr Gln Phe Arg Ile Ala 15805 15810 15815 Ala Glu Asn Arg Tyr Gly Lys Ser Thr Tyr Leu Asn Ser Glu Pro 15820 15825 15830 Thr Val Ala Gln Tyr Pro Phe Lys Val Pro Gly Pro Pro Gly Thr 15835 15840 15845 Pro Val Val Thr Leu Ser Ser Arg Asp Ser Met Glu Val Gln Trp 15850 15855 15860 Asn Glu Pro Ile Ser Asp Gly Gly Ser Arg Val Ile Gly Tyr His 15865 15870 15875 Leu Glu Arg Lys Glu Arg Asn Ser Ile Leu Trp Val Lys Leu Asn 15880 15885 15890 Lys Thr Pro Ile Pro Gln Thr Lys Phe Lys Thr Thr Gly Leu Glu 15895 15900 15905 Glu Gly Val Glu Tyr Glu Phe Arg Val Ser Ala Glu Asn Ile Val 15910 15915 15920 Gly Ile Gly Lys Pro Ser Lys Val Ser Glu Cys Tyr Val Ala Arg 15925 15930 15935 Asp Pro Cys Asp Pro Pro Gly Arg Pro Glu Ala Ile Ile Val Thr 15940 15945 15950 Arg Asn Ser Val Thr Leu Gln Trp Lys Lys Pro Thr Tyr Asp Gly 15955 15960 15965 Gly Ser Lys Ile Thr Gly Tyr Ile Val Glu Lys Lys Glu Leu Pro 15970 15975 15980 Glu Gly Arg Trp Met Lys Ala Ser Phe Thr Asn Ile Ile Asp Thr 15985 15990 15995 His Phe Glu Val Thr Gly Leu Val Glu Asp His Arg Tyr Glu Phe 16000 16005 16010 Arg Val Ile Ala Arg Asn Ala Ala Gly Val Phe Ser Glu Pro Ser 16015 16020 16025 Glu Ser Thr Gly Ala Ile Thr Ala Arg Asp Glu Val Asp Pro Pro 16030 16035 16040 Arg Ile Ser Met Asp Pro Lys Tyr Lys Asp Thr Ile Val Val His 16045 16050 16055 Ala Gly Glu Ser Phe Lys Val Asp Ala Asp Ile Tyr Gly Lys Pro 16060 16065 16070 Ile Pro Thr Ile Gln Trp Ile Lys Gly Asp Gln Glu Leu Ser Asn 16075 16080 16085 Thr Ala Arg Leu Glu Ile Lys Ser Thr Asp Phe Ala Thr Ser Leu 16090 16095 16100 Ser Val Lys Asp Ala Val Arg Val Asp Ser Gly Asn Tyr Ile Leu 16105 16110 16115 Lys Ala Lys Asn Val Ala Gly Glu Arg Ser Val Thr Val Asn Val 16120 16125 16130 Lys Val Leu Asp Arg Pro Gly Pro Pro Glu Gly Pro Val Val Ile 16135 16140 16145 Ser Gly Val Thr Ala Glu Lys Cys Thr Leu Ala Trp Lys Pro Pro 16150 16155 16160 Leu Gln Asp Gly Gly Ser Asp Ile Ile Asn Tyr Ile Val Glu Arg 16165 16170 16175 Arg Glu Thr Ser Arg Leu Val Trp Thr Val Val Asp Ala Asn Val 16180 16185 16190 Gln Thr Leu Ser Cys Lys Val Thr Lys Leu Leu Glu Gly Asn Glu 16195 16200 16205 Tyr Thr Phe Arg Ile Met Ala Val Asn Lys Tyr Gly Val Gly Glu 16210 16215 16220 Pro Leu Glu Ser Glu Pro Val Val Ala Lys Asn Pro Phe Val Val 16225 16230 16235 Pro Asp Ala Pro Lys Ala Pro Glu Val Thr Thr Val Thr Lys Asp 16240 16245 16250 Ser Met Ile Val Val Trp Glu Arg Pro Ala Ser Asp Gly Gly Ser 16255 16260 16265 Glu Ile Leu Gly Tyr Val Leu Glu Lys Arg Asp Lys Glu Gly Ile 16270 16275 16280 Arg Trp Thr Arg Cys His Lys Arg Leu Ile Gly Glu Leu Arg Leu 16285 16290 16295 Arg Val Thr Gly Leu Ile Glu Asn His Asp Tyr Glu Phe Arg Val 16300 16305 16310 Ser Ala Glu Asn Ala Ala Gly Leu Ser Glu Pro Ser Pro Pro Ser 16315 16320 16325 Ala Tyr Gln Lys Ala Cys Asp Pro Ile Tyr Lys Pro Gly Pro Pro 16330 16335 16340 Asn Asn Pro Lys Val Ile Asp Ile Thr Arg Ser Ser Val Phe Leu 16345 16350 16355 Ser Trp Ser Lys Pro Ile Tyr Asp Gly Gly Cys Glu Ile Gln Gly 16360 16365 16370 Tyr Ile Val Glu Lys Cys Asp Val Asn Val Gly Glu Trp Thr Met 16375 16380 16385 Cys Thr Pro Pro Thr Gly Ile Asn Lys Thr Asn Ile Glu Val Glu 16390 16395 16400 Lys Leu Leu Glu Lys His Glu Tyr Asn Phe Arg Ile Cys Ala Ile 16405 16410 16415 Asn Lys Ala Gly Val Gly Glu His Ala Asp Val Pro Gly Pro Ile 16420 16425 16430 Ile Val Glu Glu Lys Leu Glu Ala Pro Asp Ile Asp Leu Asp Leu 16435 16440 16445 Glu Leu Arg Lys Ile Ile Asn Ile Arg Ala Gly Gly Ser Leu Arg 16450 16455 16460 Leu Phe Val Pro Ile Lys Gly Arg Pro Thr Pro Glu Val Lys Trp 16465 16470 16475 Gly Lys Val Asp Gly Glu Ile Arg Asp Ala Ala Ile Ile Asp Val 16480 16485 16490 Thr Ser Ser Phe Thr Ser Leu Val Leu Asp Asn Val Asn Arg Tyr 16495 16500 16505 Asp Ser Gly Lys Tyr Thr Leu Thr Leu Glu Asn Ser Ser Gly Thr 16510 16515 16520 Lys Ser Ala Phe Val Thr Val Arg Val Leu Asp Thr Pro Ser Pro 16525 16530 16535 Pro Val Asn Leu Lys Val Thr Glu Ile Thr Lys Asp Ser Val Ser 16540 16545 16550 Ile Thr Trp Glu Pro Pro Leu Leu Asp Gly Gly Ser Lys Ile Lys 16555 16560 16565 Asn Tyr Ile Val Glu Lys Arg Glu Ala Thr Arg Lys Ser Tyr Ala 16570 16575 16580 Ala Val Val Thr Asn Cys His Lys Asn Ser Trp Lys Ile Asp Gln 16585 16590 16595 Leu Gln Glu Gly Cys Ser Tyr Tyr Phe Arg Val Thr Ala Glu Asn 16600 16605 16610 Glu Tyr Gly Ile Gly Leu Pro Ala Gln Thr Ala Asp Pro Ile Lys 16615 16620 16625 Val Ala Glu Val Pro Gln Pro Pro Gly Lys Ile Thr Val Asp Asp 16630 16635 16640 Val Thr Arg Asn Ser Val Ser Leu Ser Trp Thr Lys Pro Glu His 16645 16650 16655 Asp Gly Gly Ser Lys Ile Ile Gln Tyr Ile Val Glu Met Gln Ala 16660 16665 16670 Lys His Ser Glu Lys Trp Ser Glu Cys Ala Arg Val Lys Ser Leu 16675 16680 16685 Gln Ala Val Ile Thr Asn Leu Thr Gln Gly Glu Glu Tyr Leu Phe 16690 16695 16700 Arg Val Val Ala Val Asn Glu Lys Gly Arg Ser Asp Pro Arg Ser 16705 16710 16715 Leu Ala Val Pro Ile Val Ala Lys Asp Leu Val Ile Glu Pro Asp 16720 16725 16730 Val Lys Pro Ala Phe Ser Ser Tyr Ser Val Gln Val Gly Gln Asp 16735 16740 16745 Leu Lys Ile Glu Val Pro Ile Ser Gly Arg Pro Lys Pro Thr Ile 16750 16755 16760 Thr Trp Thr Lys Asp Gly Leu Pro Leu Lys Gln Thr Thr Arg Ile 16765 16770 16775 Asn Val Thr Asp Ser Leu Asp Leu Thr Thr Leu Ser Ile Lys Glu 16780 16785 16790 Thr His Lys Asp Asp Gly Gly Gln Tyr Gly Ile Thr Val Ala Asn 16795 16800 16805 Val Val Gly Gln Lys Thr Ala Ser Ile Glu Ile Val Thr Leu Asp 16810 16815 16820 Lys Pro Asp Pro Pro Lys Gly Pro Val Lys Phe Asp Asp Val Ser 16825 16830 16835 Ala Glu Ser Ile Thr Leu Ser Trp Asn Pro Pro Leu Tyr Thr Gly 16840 16845 16850 Gly Cys Gln Ile Thr Asn Tyr Ile Val Gln Lys Arg Asp Thr Thr 16855 16860 16865 Thr Thr Val Trp Asp Val Val Ser Ala Thr Val Ala Arg Thr Thr 16870 16875 16880 Leu Lys Val Thr Lys Leu Lys Thr Gly Thr Glu Tyr Gln Phe Arg 16885 16890 16895 Ile Phe Ala Glu Asn Arg Tyr Gly Gln Ser Phe Ala Leu Glu Ser 16900 16905 16910 Asp Pro Ile Val Ala Gln Tyr Pro Tyr Lys Glu Pro Gly Pro Pro 16915 16920 16925 Gly Thr Pro Phe Ala Thr Ala Ile Ser Lys Asp Ser Met Val Ile 16930 16935 16940 Gln Trp His Glu Pro Val Asn Asn Gly Gly Ser Pro Val Ile Gly 16945 16950 16955 Tyr His Leu Glu Arg Lys Glu Arg Asn Ser Ile Leu Trp Thr Lys 16960 16965 16970 Val Asn Lys Thr Ile Ile His Asp Thr Gln Phe Lys Ala Gln Asn 16975 16980 16985 Leu Glu Glu Gly Ile Glu Tyr Glu Phe Arg Val Tyr Ala Glu Asn 16990 16995 17000 Ile Val Gly Val Gly Lys Ala Ser Lys Asn Ser Glu Cys Tyr Val 17005 17010 17015 Ala Arg Asp Pro Cys Asp Pro Pro Gly Thr Pro Glu Pro Ile Met 17020 17025 17030 Val Lys Arg Asn Glu Ile Thr Leu Gln Trp Thr Lys Pro Val Tyr 17035 17040 17045 Asp Gly Gly Ser Met Ile Thr Gly Tyr Ile Val Glu Lys Arg Asp 17050 17055 17060 Leu Pro Asp Gly Arg Trp Met Lys Ala Ser Phe Thr Asn Val Ile 17065 17070 17075 Glu Thr Gln Phe Thr Val Ser Gly Leu Thr Glu Asp Gln Arg Tyr 17080 17085 17090 Glu Phe Arg Val Ile Ala Lys Asn Ala Ala Gly Ala Ile Ser Lys 17095 17100 17105 Pro Ser Asp Ser Thr Gly Pro Ile Thr Ala Lys Asp Glu Val Glu 17110 17115 17120 Leu Pro Arg Ile Ser Met Asp Pro Lys Phe Arg Asp Thr Ile Val 17125 17130 17135 Val Asn Ala Gly Glu Thr Phe Arg Leu Glu Ala Asp Val His Gly 17140 17145 17150 Lys Pro Leu Pro Thr Ile Glu Trp Leu Arg Gly Asp Lys Glu Ile 17155 17160 17165 Glu Glu Ser Ala Arg Cys Glu Ile Lys Asn Thr Asp Phe Lys Ala 17170 17175 17180 Leu Leu Ile Val Lys Asp Ala Ile Arg Ile Asp Gly Gly Gln Tyr 17185 17190 17195 Ile Leu Arg Ala Ser Asn Val Ala Gly Ser Lys Ser Phe Pro Val 17200 17205 17210 Asn Val Lys Val Leu Asp Arg Pro Gly Pro Pro Glu Gly Pro Val 17215 17220 17225 Gln Val Thr Gly Val Thr Ser Glu Lys Cys Ser Leu Thr Trp Ser 17230 17235 17240 Pro Pro Leu Gln Asp Gly Gly Ser Asp Ile Ser His Tyr Val Val 17245 17250 17255 Glu Lys Arg Glu Thr Ser Arg Leu Ala Trp Thr Val Val Ala Ser 17260 17265 17270 Glu Val Val Thr Asn Ser Leu Lys Val Thr Lys Leu Leu Glu Gly 17275 17280 17285 Asn Glu Tyr Val Phe Arg Ile Met Ala Val Asn Lys Tyr Gly Val 17290 17295 17300 Gly Glu Pro Leu Glu Ser Ala Pro Val Leu Met Lys Asn Pro Phe 17305 17310 17315 Val Leu Pro Gly Pro Pro Lys Ser Leu Glu Val Thr Asn Ile Ala 17320 17325 17330 Lys Asp Ser Met Thr Val Cys Trp Asn Arg Pro Asp Ser Asp Gly 17335 17340 17345 Gly Ser Glu Ile Ile Gly Tyr Ile Val Glu Lys Arg Asp Arg Ser 17350 17355 17360 Gly Ile Arg Trp Ile Lys Cys Asn Lys Arg Arg Ile Thr Asp Leu 17365 17370 17375 Arg Leu Arg Val Thr Gly Leu Thr Glu Asp His Glu Tyr Glu Phe 17380 17385 17390 Arg Val Ser Ala Glu Asn Ala Ala Gly Val Gly Glu Pro Ser Pro 17395 17400 17405 Ala Thr Val Tyr Tyr Lys Ala Cys Asp Pro Val Phe Lys Pro Gly 17410 17415 17420 Pro Pro Thr Asn Ala His Ile Val Asp Thr Thr Lys Asn Ser Ile 17425 17430 17435 Thr Leu Ala Trp Gly Lys Pro Ile Tyr Asp Gly Gly Ser Glu Ile 17440 17445 17450 Leu Gly Tyr Val Val Glu Ile Cys Lys Ala Asp Glu Glu Glu Trp 17455 17460 17465 Gln Ile Val Thr Pro Gln Thr Gly Leu Arg Val Thr Arg Phe Glu 17470 17475 17480 Ile Ser Lys Leu Thr Glu His Gln Glu Tyr Lys Ile Arg Val Cys 17485 17490 17495 Ala Leu Asn Lys Val Gly Leu Gly Glu Ala Thr Ser Val Pro Gly 17500 17505 17510 Thr Val Lys Pro Glu Asp Lys Leu Glu Ala Pro Glu Leu Asp Leu 17515 17520 17525 Asp Ser Glu Leu Arg Lys Gly Ile Val Val Arg Ala Gly Gly Ser 17530 17535 17540 Ala Arg Ile His Ile Pro Phe Lys Gly Arg Pro Met Pro Glu Ile 17545 17550 17555 Thr Trp Ser Arg Glu Glu Gly Glu Phe Thr Asp Lys Val Gln Ile 17560 17565 17570 Glu Lys Gly Val Asn Tyr Thr Gln Leu Ser Ile Asp Asn Cys Asp 17575 17580 17585 Arg Asn Asp Ala Gly Lys Tyr Ile Leu Lys Leu Glu Asn Ser Ser 17590 17595 17600 Gly Ser Lys Ser Ala Phe Val Thr Val Lys Val Leu Asp Thr Pro 17605 17610 17615 Gly Pro Pro Gln Asn Leu Ala Val Lys Glu Val Arg Lys Asp Ser 17620 17625 17630 Ala Phe Leu Val Trp Glu Pro Pro Ile Ile Asp Gly Gly Ala Lys 17635 17640 17645 Val Lys Asn Tyr Val Ile Asp Lys Arg Glu Ser Thr Arg Lys Ala 17650 17655 17660 Tyr Ala Asn Val Ser Ser Lys Cys Ser Lys Thr Ser Phe Lys Val 17665 17670 17675 Glu Asn Leu Thr Glu Gly Ala Ile Tyr Tyr Phe Arg Val Met Ala 17680 17685 17690 Glu Asn Glu Phe Gly Val Gly Val Pro Val Glu Thr Val Asp Ala 17695 17700 17705 Val Lys Ala Ala Glu Pro Pro Ser Pro Pro Gly Lys Val Thr Leu 17710 17715 17720 Thr Asp Val Ser Gln Thr Ser Ala Ser Leu Met Trp Glu Lys Pro 17725 17730 17735 Glu His Asp Gly Gly Ser Arg Val Leu Gly Tyr Val Val Glu Met 17740 17745 17750 Gln Pro Lys Gly Thr Glu Lys Trp Ser Ile Val Ala Glu Ser Lys 17755 17760 17765 Val Cys Asn Ala Val Val Thr Gly Leu Ser Ser Gly Gln Glu Tyr 17770 17775 17780 Gln Phe Arg Val Lys Ala Tyr Asn Glu Lys Gly Lys Ser Asp Pro 17785 17790 17795 Arg Val Leu Gly Val Pro Val Ile Ala Lys Asp Leu Thr Ile Gln 17800 17805 17810 Pro Ser Leu Lys Leu Pro Phe Asn Thr Tyr Ser Ile Gln Ala Gly 17815 17820 17825 Glu Asp Leu Lys Ile Glu Ile Pro Val Ile Gly Arg Pro Arg Pro 17830 17835 17840 Asn Ile Ser Trp Val Lys Asp Gly Glu Pro Leu Lys Gln Thr Thr 17845 17850 17855 Arg Val Asn Val Glu Glu Thr Ala Thr Ser Thr Val Leu His Ile 17860 17865 17870 Lys Glu Gly Asn Lys Asp Asp Phe Gly Lys Tyr Thr Val Thr Ala 17875 17880 17885 Thr Asn Ser Ala Gly Thr Ala Thr Glu Asn Leu Ser Val Ile Val 17890 17895 17900 Leu Glu Lys Pro Gly Pro Pro Val Gly Pro Val Arg Phe Asp Glu 17905 17910 17915 Val Ser Ala Asp Phe Val Val Ile Ser Trp Glu Pro Pro Ala Tyr 17920 17925 17930 Thr Gly Gly Cys Gln Ile Ser Asn Tyr Ile Val Glu Lys Arg Asp 17935 17940 17945 Thr Thr Thr Thr Thr Trp His Met Val Ser Ala Thr Val Ala Arg 17950 17955 17960 Thr Thr Ile Lys Ile Thr Lys Leu Lys Thr Gly Thr Glu Tyr Gln 17965 17970 17975 Phe Arg Ile Phe Ala Glu Asn Arg Tyr Gly Lys Ser Ala Pro Leu 17980 17985 17990 Asp Ser Lys Ala Val Ile Val Gln Tyr Pro Phe Lys Glu Pro Gly 17995 18000 18005 Pro Pro Gly Thr Pro Phe Val Thr Ser Ile Ser Lys Asp Gln Met 18010 18015 18020 Leu Val Gln Trp His Glu Pro Val Asn Asp Gly Gly Thr Lys Ile 18025 18030 18035 Ile Gly Tyr His Leu Glu Gln Lys Glu Lys Asn Ser Ile Leu Trp 18040 18045 18050 Val Lys Leu Asn Lys Thr Pro Ile Gln Asp Thr Lys Phe Lys Thr 18055 18060 18065 Thr Gly Leu Asp Glu Gly Leu Glu Tyr Glu Phe Lys Val Ser Ala 18070 18075 18080 Glu Asn Ile Val Gly Ile Gly Lys Pro Ser Lys Val Ser Glu Cys 18085 18090 18095 Phe Val Ala Arg Asp Pro Cys Asp Pro Pro Gly Arg Pro Glu Ala 18100 18105 18110 Ile Val Ile Thr Arg Asn Asn Val Thr Leu Lys Trp Lys Lys Pro 18115 18120 18125 Ala Tyr Asp Gly Gly Ser Lys Ile Thr Gly Tyr Ile Val Glu Lys 18130 18135 18140 Lys Asp Leu Pro Asp Gly Arg Trp Met Lys Ala Ser Phe Thr Asn 18145 18150 18155 Val Leu Glu Thr Glu Phe Thr Val Ser Gly Leu Val Glu Asp Gln 18160 18165 18170 Arg Tyr Glu Phe Arg Val Ile Ala Arg Asn Ala Ala Gly Asn Phe 18175 18180 18185 Ser Glu Pro Ser Asp Ser Ser Gly Ala Ile Thr Ala Arg Asp Glu 18190 18195 18200 Ile Asp Ala Pro Asn Ala Ser Leu Asp Pro Lys Tyr Lys Asp Val 18205 18210 18215 Ile Val Val His Ala Gly Glu Thr Phe Val Leu Glu Ala Asp Ile 18220 18225 18230 Arg Gly Lys Pro Ile Pro Asp Val Val Trp Ser Lys Asp Gly Lys 18235 18240 18245 Glu Leu Glu Glu Thr Ala Ala Arg Met Glu Ile Lys Ser Thr Ile 18250 18255 18260 Gln Lys Thr Thr Leu Val Val Lys Asp Cys Ile Arg Thr Asp Gly 18265 18270 18275 Gly Gln Tyr Ile Leu Lys Leu Ser Asn Val Gly Gly Thr Lys Ser 18280 18285 18290 Ile Pro Ile Thr Val Lys Val Leu Asp Arg Pro Gly Ser Pro Glu 18295 18300 18305 Gly Pro Leu Lys Val Thr Gly Val Thr Ala Glu Lys Cys Tyr Leu 18310 18315 18320 Ala Trp Asn Pro Pro Leu Gln Asp Gly Gly Ala Asn Ile Ser His 18325 18330 18335 Tyr Ile Ile Glu Lys Arg Glu Thr Ser Arg Leu Ser Trp Thr Gln 18340 18345 18350 Val Ser Thr Glu Val Gln Ala Leu Asn Tyr Lys Val Thr Lys Leu 18355 18360 18365 Leu Pro Gly Asn Glu Tyr Ile Phe Arg Val Met Ala Val Asn Lys 18370 18375 18380 Tyr Gly Ile Gly Glu Pro Leu Glu Ser Gly Pro Val Thr Ala Cys 18385 18390 18395 Asn Pro Tyr Lys Pro Pro Gly Pro Pro Ser Thr Pro Glu Val Ser 18400 18405 18410 Ala Ile Thr Lys Asp Ser Met Val Val Thr Trp Ala Arg Pro Val 18415 18420 18425 Asp Asp Gly Gly Thr Glu Ile Glu Gly Tyr Ile Leu Glu Lys Arg 18430 18435 18440 Asp Lys Glu Gly Val Arg Trp Thr Lys Cys Asn Lys Lys Thr Leu 18445 18450 18455 Thr Asp Leu Arg Leu Arg Val Thr Gly Leu Thr Glu Gly His Ser 18460 18465 18470 Tyr Glu Phe Arg Val Ala Ala Glu Asn Ala Ala Gly Val Gly Glu 18475 18480 18485 Pro Ser Glu Pro Ser Val Phe Tyr Arg Ala Cys Asp Ala Leu Tyr 18490 18495 18500 Pro Pro Gly Pro Pro Ser Asn Pro Lys Val Thr Asp Thr Ser Arg 18505 18510 18515 Ser Ser Val Ser Leu Ala Trp Ser Lys Pro Ile Tyr Asp Gly Gly 18520 18525 18530 Ala Pro Val Lys Gly Tyr Val Val Glu Val Lys Glu Ala Ala Ala 18535 18540 18545 Asp Glu Trp Thr Thr Cys Thr Pro Pro Thr Gly Leu Gln Gly Lys 18550 18555 18560 Gln Phe Thr Val Thr Lys Leu Lys Glu Asn Thr Glu Tyr Asn Phe 18565 18570 18575 Arg Ile Cys Ala Ile Asn Ser Glu Gly Val Gly Glu Pro Ala Thr 18580 18585 18590 Leu Pro Gly Ser Val Val Ala Gln Glu Arg Ile Glu Pro Pro Glu 18595 18600 18605 Ile Glu Leu Asp Ala Asp Leu Arg Lys Val Val Val Leu Arg Ala 18610 18615 18620 Ser Ala Thr Leu Arg Leu Phe Val Thr Ile Lys Gly Arg Pro Glu 18625 18630 18635 Pro Glu Val Lys Trp Glu Lys Ala Glu Gly Ile Leu Thr Asp Arg 18640 18645 18650 Ala Gln Ile Glu Val Thr Ser Ser Phe Thr Met Leu Val Ile Asp 18655 18660 18665 Asn Val Thr Arg Phe Asp Ser Gly Arg Tyr Asn Leu Thr Leu Glu 18670 18675 18680 Asn Asn Ser Gly Ser Lys Thr Ala Phe Val Asn Val Arg Val Leu 18685 18690 18695 Asp Ser Pro Ser Ala Pro Val Asn Leu Thr Ile Arg Glu Val Lys 18700 18705 18710 Lys Asp Ser Val Thr Leu Ser Trp Glu Pro Pro Leu Ile Asp Gly 18715 18720 18725 Gly Ala Lys Ile Thr Asn Tyr Ile Val Glu Lys Arg Glu Thr Thr 18730 18735 18740 Arg Lys Ala Tyr Ala Thr Ile Thr Asn Asn Cys Thr Lys Thr Thr 18745 18750 18755 Phe Arg Ile Glu Asn Leu Gln Glu Gly Cys Ser Tyr Tyr Phe Arg 18760 18765 18770 Val Leu Ala Ser Asn Glu Tyr Gly Ile Gly Leu Pro Ala Glu Thr 18775 18780 18785 Thr Glu Pro Val Lys Val Ser Glu Pro Pro Leu Pro Pro Gly Arg 18790 18795 18800 Val Thr Leu Val Asp Val Thr Arg Asn Thr Ala Thr Ile Lys Trp 18805 18810 18815 Glu Lys Pro Glu Ser Asp Gly Gly Ser Lys Ile Thr Gly Tyr Val 18820 18825 18830 Val Glu Met Gln Thr Lys Gly Ser Glu Lys Trp Ser Thr Cys Thr 18835 18840 18845 Gln Val Lys Thr Leu Glu Ala Thr Ile Ser Gly Leu Thr Ala Gly 18850 18855 18860 Glu Glu Tyr Val Phe Arg Val Ala Ala Val Asn Glu Lys Gly Arg 18865 18870 18875 Ser Asp Pro Arg Gln Leu Gly Val Pro Val Ile Ala Arg Asp Ile 18880 18885 18890 Glu Ile Lys Pro Ser Val Glu Leu Pro Phe His Thr Phe Asn Val 18895 18900 18905 Lys Ala Arg Glu Gln Leu Lys Ile Asp Val Pro Phe Lys Gly Arg 18910 18915 18920 Pro Gln Ala Thr Val Asn Trp Arg Lys Asp Gly Gln Thr Leu Lys 18925 18930 18935 Glu Thr Thr Arg Val Asn Val Ser Ser Ser Lys Thr Val Thr Ser 18940 18945 18950 Leu Ser Ile Lys Glu Ala Ser Lys Glu Asp Val Gly Thr Tyr Glu 18955 18960 18965 Leu Cys Val Ser Asn Ser Ala Gly Ser Ile Thr Val Pro Ile Thr 18970 18975 18980 Ile Ile Val Leu Asp Arg Pro Gly Pro Pro Gly Pro Ile Arg Ile 18985 18990 18995 Asp Glu Val Ser Cys Asp Ser Ile Thr Ile Ser Trp Asn Pro Pro 19000 19005 19010 Glu Tyr Asp Gly Gly Cys Gln Ile Ser Asn Tyr Ile Val Glu Lys 19015 19020 19025 Lys Glu Thr Thr Ser Thr Thr Trp His Ile Val Ser Gln Ala Val 19030 19035 19040 Ala Arg Thr Ser Ile Lys Ile Val Arg Leu Thr Thr Gly Ser Glu 19045 19050 19055 Tyr Gln Phe Arg Val Cys Ala Glu Asn Arg Tyr Gly Lys Ser Ser 19060 19065 19070 Tyr Ser Glu Ser Ser Ala Val Val Ala Glu Tyr Pro Phe Ser Pro 19075 19080 19085 Pro Gly Pro Pro Gly Thr Pro Lys Val Val His Ala Thr Lys Ser 19090 19095 19100 Thr Met Leu Val Thr Trp Gln Val Pro Val Asn Asp Gly Gly Ser 19105 19110 19115 Arg Val Ile Gly Tyr His Leu Glu Tyr Lys Glu Arg Ser Ser Ile 19120 19125 19130 Leu Trp Ser Lys Ala Asn Lys Ile Leu Ile Ala Asp Thr Gln Val 19135 19140 19145 Lys Val Ser Gly Leu Asp Glu Gly Leu Met Tyr Glu Tyr Arg Val 19150 19155 19160 Tyr Ala Glu Asn Ile Ala Gly Ile Gly Lys Cys Ser Lys Ser Cys 19165 19170 19175 Glu Pro Val Pro Ala Arg Asp Pro Cys Asp Pro Pro Gly Gln Pro 19180 19185 19190 Glu Val Thr Asn Ile Thr Arg Lys Ser Val Ser Leu Lys Trp Ser 19195 19200 19205 Lys Pro His Tyr Asp Gly Gly Ala Lys Ile Thr Gly Tyr Ile Val 19210 19215 19220 Glu Arg Arg Glu Leu Pro Asp Gly Arg Trp Leu Lys Cys Asn Tyr 19225 19230 19235 Thr Asn Ile Gln Glu Thr Tyr Phe Glu Val Thr Glu Leu Thr Glu 19240 19245 19250 Asp Gln Arg Tyr Glu Phe Arg Val Phe Ala Arg Asn Ala Ala Asp 19255 19260 19265 Ser Val Ser Glu Pro Ser Glu Ser Thr Gly Pro Ile Ile Val Lys 19270 19275 19280 Asp Asp Val Glu Pro Pro Arg Val Met Met Asp Val Lys Phe Arg 19285 19290 19295 Asp Val Ile Val Val Lys Ala Gly Glu Val Leu Lys Ile Asn Ala 19300 19305 19310 Asp Ile Ala Gly Arg Pro Leu Pro Val Ile Ser Trp Ala Lys Asp 19315 19320 19325 Gly Ile Glu Ile Glu Glu Arg Ala Arg Thr Glu Ile Ile Ser Thr 19330 19335 19340 Asp Asn His Thr Leu Leu Thr Val Lys Asp Cys Ile Arg Arg Asp 19345 19350 19355 Thr Gly Gln Tyr Val Leu Thr Leu Lys Asn Val Ala Gly Thr Arg 19360 19365 19370 Ser Val Ala Val Asn Cys Lys Val Leu Asp Lys Pro Gly Pro Pro 19375 19380 19385 Ala Gly Pro Leu Glu Ile Asn Gly Leu Thr Ala Glu Lys Cys Ser 19390 19395 19400 Leu Ser Trp Gly Arg Pro Gln Glu Asp Gly Gly Ala Asp Ile Asp 19405 19410 19415 Tyr Tyr His Arg Lys Lys Arg Glu Thr Ser His Leu Ala Trp Thr 19420 19425 19430 Ile Cys Glu Gly Glu Leu Gln Met Thr Ser Cys Lys Val Thr Lys 19435 19440 19445 Leu Leu Lys Gly Asn Glu Tyr Ile Phe Arg Val Thr Gly Val Asn 19450 19455 19460 Lys Tyr Gly Val Gly Glu Pro Leu Glu Ser Val Ala Ile Lys Ala 19465 19470 19475 Leu Asp Pro Phe Thr Val Pro Ser Pro Pro Thr Ser Leu Glu Ile 19480 19485 19490 Thr Ser Val Thr Lys Glu Ser Met Thr Leu Cys Trp Ser Arg Pro 19495 19500 19505 Glu Ser Asp Gly Gly Ser Glu Ile Ser Gly Tyr Ile Ile Glu Arg 19510 19515 19520 Arg Glu Lys Asn Ser Leu Arg Trp Val Arg Val Asn Lys Lys Pro 19525 19530 19535 Val Tyr Asp Leu Arg Val Lys Ser Thr Gly Leu Arg Glu Gly Cys 19540 19545 19550 Glu Tyr Glu Tyr Arg Val Tyr Ala Glu Asn Ala Ala Gly Leu Ser 19555 19560 19565 Leu Pro Ser Glu Thr Ser Pro Leu Ile Arg Ala Glu Asp Pro Val 19570 19575 19580 Phe Leu Pro Ser Pro Pro Ser Lys Pro Lys Ile Val Asp Ser Gly 19585 19590 19595 Lys Thr Thr Ile Thr Ile Ala Trp Val Lys Pro Leu Phe Asp Gly 19600 19605 19610 Gly Ala Pro Ile Thr Gly Tyr Thr Val Glu Tyr Lys Lys Ser Asp 19615 19620 19625 Asp Thr Asp Trp Lys Thr Ser Ile Gln Ser Leu Arg Gly Thr Glu 19630 19635 19640 Tyr Thr Ile Ser Gly Leu Thr Thr Gly Ala Glu Tyr Val Phe Arg 19645 19650 19655 Val Lys Ser Val Asn Lys Val Gly Ala Ser Asp Pro Ser Asp Ser 19660 19665 19670 Ser Asp Pro Gln Ile Ala Lys Glu Arg Glu Glu Glu Pro Leu Phe 19675 19680 19685 Asp Ile Asp Ser Glu Met Arg Lys Thr Leu Ile Val Lys Ala Gly 19690 19695 19700 Ala Ser Phe Thr Met Thr Val Pro Phe Arg Gly Arg Pro Val Pro 19705 19710 19715 Asn Val Leu Trp Ser Lys Pro Asp Thr Asp Leu Arg Thr Arg Ala 19720 19725 19730 Tyr Val Asp Thr Thr Asp Ser Arg Thr Ser Leu Thr Ile Glu Asn 19735 19740 19745 Ala Asn Arg Asn Asp Ser Gly Lys Tyr Thr Leu Thr Ile Gln Asn 19750 19755 19760 Val Leu Ser Ala Ala Ser Leu Thr Leu Val Val Lys Val Leu Asp 19765 19770 19775 Thr Pro Gly Pro Pro Thr Asn Ile Thr Val Gln Asp Val Thr Lys 19780 19785 19790 Glu Ser Ala Val Leu Ser Trp Asp Val Pro Glu Asn Asp Gly Gly 19795 19800 19805 Ala Pro Val Lys Asn Tyr His Ile Glu Lys Arg Glu Ala Ser Lys 19810 19815 19820 Lys Ala Trp Val Ser Val Thr Asn Asn Cys Asn Arg Leu Ser Tyr 19825 19830 19835 Lys Val Thr Asn Leu Gln Glu Gly Ala Ile Tyr Tyr Phe Arg Val 19840 19845 19850 Ser Gly Glu Asn Glu Phe Gly Val Gly Ile Pro Ala Glu Thr Lys 19855 19860 19865 Glu Gly Val Lys Ile Thr Glu Lys Pro Ser Pro Pro Glu Lys Leu 19870 19875 19880 Gly Val Thr Ser Ile Ser Lys Asp Ser Val Ser Leu Thr Trp Leu 19885 19890 19895 Lys Pro Glu His Asp Gly Gly Ser Arg Ile Val His Tyr Val Val 19900 19905 19910 Glu Ala Leu Glu Lys Gly Gln Lys Asn Trp Val Lys Cys Ala Val 19915 19920 19925 Ala Lys Ser Thr His His Val Val Ser Gly Leu Arg Glu Asn Ser 19930 19935 19940 Glu Tyr Phe Phe Arg Val Phe Ala Glu Asn Gln Ala Gly Leu Ser 19945 19950 19955 Asp Pro Arg Glu Leu Leu Leu Pro Val Leu Ile Lys Glu Gln Leu 19960 19965 19970 Glu Pro Pro Glu Ile Asp Met Lys Asn Phe Pro Ser His Thr Val 19975 19980 19985 Tyr Val Arg Ala Gly Ser Asn Leu Lys Val Asp Ile Pro Ile Ser 19990 19995 20000 Gly Lys Pro Leu Pro Lys Val Thr Leu Ser Arg Asp Gly Val Pro 20005 20010 20015 Leu Lys Ala Thr Met Arg Phe Asn Thr Glu Ile Thr Ala Glu Asn 20020 20025 20030 Leu Thr Ile Asn Leu Lys Glu Ser Val Thr Ala Asp Ala Gly Arg 20035 20040 20045 Tyr Glu Ile Thr Ala Ala Asn Ser Ser Gly Thr Thr Lys Ala Phe 20050 20055 20060 Ile Asn Ile Val Val Leu Asp Arg Pro Gly Pro Pro Thr Gly Pro 20065 20070 20075 Val Val Ile Ser Asp Ile Thr Glu Glu Ser Val Thr Leu Lys Trp 20080 20085 20090 Glu Pro Pro Lys Tyr Asp Gly Gly Ser Gln Val Thr Asn Tyr Ile 20095 20100 20105 Leu Leu Lys Arg Glu Thr Ser Thr Ala Val Trp Thr Glu Val Ser 20110 20115 20120 Ala Thr Val Ala Arg Thr Met Met Lys Val Met Lys Leu Thr Thr 20125 20130 20135 Gly Glu Glu Tyr Gln Phe Arg Ile Lys Ala Glu Asn Arg Phe Gly 20140 20145 20150 Ile Ser Asp His Ile Asp Ser Ala Cys Val Thr Val Lys Leu Pro 20155 20160 20165 Tyr Thr Thr Pro Gly Pro Pro Ser Thr Pro Trp Val Thr Asn Val 20170 20175 20180 Thr Arg Glu Ser Ile Thr Val Gly Trp His Glu Pro Val Ser Asn 20185 20190 20195 Gly Gly Ser Ala Val Val Gly Tyr His Leu Glu Met Lys Asp Arg 20200 20205 20210 Asn Ser Ile Leu Trp Gln Lys Ala Asn Lys Leu Val Ile Arg Thr 20215 20220 20225 Thr His Phe Lys Val Thr Thr Ile Ser Ala Gly Leu Ile Tyr Glu 20230 20235 20240 Phe Arg Val Tyr Ala Glu Asn Ala Ala Gly Val Gly Lys Pro Ser 20245 20250 20255 His Pro Ser Glu Pro Val Leu Ala Ile Asp Ala Cys Glu Pro Pro 20260 20265 20270 Arg Asn Val Arg Ile Thr Asp Ile Ser Lys Asn Ser Val Ser Leu 20275 20280 20285 Ser Trp Gln Gln Pro Ala Phe Asp Gly Gly Ser Lys Ile Thr Gly 20290 20295 20300 Tyr Ile Val Glu Arg Arg Asp Leu Pro Asp Gly Arg Trp Thr Lys 20305 20310 20315 Ala Ser Phe Thr Asn Val Thr Glu Thr Gln Phe Thr Ile Ser Gly 20320 20325 20330 Leu Thr Gln Asn Ser Gln Tyr Glu Phe Arg Val Phe Ala Arg Asn 20335 20340 20345 Ala Val Gly Ser Ile Ser Asn Pro Ser Glu Val Val Gly Pro Ile 20350 20355 20360 Thr Cys Ile Asp Ser Tyr Gly Gly Pro Val Ile Asp Leu Pro Leu 20365 20370 20375 Glu Tyr Thr Glu Val Val Lys Tyr Arg Ala Gly Thr Ser Val Lys 20380 20385 20390 Leu Arg Ala Gly Ile Ser Gly Lys Pro Ala Pro Thr Ile Glu Trp 20395 20400 20405 Tyr Lys Asp Asp Lys Glu Leu Gln Thr Asn Ala Leu Val Cys Val 20410 20415 20420 Glu Asn Thr Thr Asp Leu Ala Ser Ile Leu Ile Lys Asp Ala Asp 20425 20430 20435 Arg Leu Asn Ser Gly Cys Tyr Glu Leu Lys Leu Arg Asn Ala Met 20440 20445 20450 Ala Ser Ala Ser Ala Thr Ile Arg Val Gln Ile Leu Asp Lys Pro 20455 20460 20465 Gly Pro Pro Gly Gly Pro Ile Glu Phe Lys Thr Val Thr Ala Glu 20470 20475 20480 Lys Ile Thr Leu Leu Trp Arg Pro Pro Ala Asp Asp Gly Gly Ala 20485 20490 20495 Lys Ile Thr His Tyr Ile Val Glu Lys Arg Glu Thr Ser Arg Val 20500 20505 20510 Val Trp Ser Met Val Ser Glu His Leu Glu Glu Cys Ile Ile Thr 20515 20520 20525 Thr Thr Lys Ile Ile Lys Gly Asn Glu Tyr Ile Phe Arg Val Arg 20530 20535 20540 Ala Val Asn Lys Tyr Gly Ile Gly Glu Pro Leu Glu Ser Asp Ser 20545 20550 20555 Val Val Ala Lys Asn Ala Phe Val Thr Pro Gly Pro Pro Gly Ile 20560 20565 20570 Pro Glu Val Thr Lys Ile Thr Lys Asn Ser Met Thr Val Val Trp 20575 20580 20585 Ser Arg Pro Ile Ala Asp Gly Gly Ser Asp Ile Ser Gly Tyr Phe 20590 20595 20600 Leu Glu Lys Arg Asp Lys Lys Ser Leu Gly Trp Phe Lys Val Leu 20605 20610 20615 Lys Glu Thr Ile Arg Asp Thr Arg Gln Lys Val Thr Gly Leu Thr 20620 20625 20630 Glu Asn Ser Asp Tyr Gln Tyr Arg Val Cys Ala Val Asn Ala Ala 20635 20640 20645 Gly Gln Gly Pro Phe Ser Glu Pro Ser Glu Phe Tyr Lys Ala Ala 20650 20655 20660 Asp Pro Ile Asp Pro Pro Gly Pro Pro Ala Lys Ile Arg Ile Ala 20665 20670 20675 Asp Ser Thr Lys Ser Ser Ile Thr Leu Gly Trp Ser Lys Pro Val 20680 20685 20690 Tyr Asp Gly Gly Ser Ala Val Thr Gly Tyr Val Val Glu Ile Arg 20695 20700 20705 Gln Gly Glu Glu Glu Glu Trp Thr Thr Val Ser Thr Lys Gly Glu 20710 20715 20720 Val Arg Thr Thr Glu Tyr Val Val Ser Asn Leu Lys Pro Gly Val 20725 20730 20735 Asn Tyr Tyr Phe Arg Val Ser Ala Val Asn Cys Ala Gly Gln Gly 20740 20745 20750 Glu Pro Ile Glu Met Asn Glu Pro Val Gln Ala Lys Asp Ile Leu 20755 20760 20765 Glu Ala Pro Glu Ile Asp Leu Asp Val Ala Leu Arg Thr Ser Val 20770 20775 20780 Ile Ala Lys Ala Gly Glu Asp Val Gln Val Leu Ile Pro Phe Lys 20785 20790 20795 Gly Arg Pro Pro Pro Thr Val Thr Trp Arg Lys Asp Glu Lys Asn 20800 20805 20810 Leu Gly Ser Asp Ala Arg Tyr Ser Ile Glu Asn Thr Asp Ser Ser 20815 20820 20825 Ser Leu Leu Thr Ile Pro Gln Val Thr Arg Asn Asp Thr Gly Lys 20830 20835 20840 Tyr Ile Leu Thr Ile Glu Asn Gly Val Gly Glu Pro Lys Ser Ser 20845 20850 20855 Thr Val Ser Val Lys Val Leu Asp Thr Pro Ala Ala Cys Gln Lys 20860 20865 20870 Leu Gln Val Lys His Val Ser Arg Gly Thr Val Thr Leu Leu Trp 20875 20880 20885 Asp Pro Pro Leu Ile Asp Gly Gly Ser Pro Ile Ile Asn Tyr Val 20890 20895 20900 Ile Glu Lys Arg Asp Ala Thr Lys Arg Thr Trp Ser Val Val Ser 20905 20910 20915 His Lys Cys Ser Ser Thr Ser Phe Lys Leu Ile Asp Leu Ser Glu 20920 20925 20930 Lys Thr Pro Phe Phe Phe Arg Val Leu Ala Glu Asn Glu Ile Gly 20935 20940 20945 Ile Gly Glu Pro Cys Glu Thr Thr Glu Pro Val Lys Ala Ala Glu 20950 20955 20960 Val Pro Ala Pro Ile Arg Asp Leu Ser Met Lys Asp Ser Thr Lys 20965 20970 20975 Thr Ser Val Ile Leu Ser Trp Thr Lys Pro Asp Phe Asp Gly Gly 20980 20985 20990 Ser Val Ile Thr Glu Tyr Val Val Glu Arg Lys Gly Lys Gly Glu 20995 21000 21005 Gln Thr Trp Ser His Ala Gly Ile Ser Lys Thr Cys Glu Ile Glu 21010 21015 21020 Val Ser Gln Leu Lys Glu Gln Ser Val Leu Glu Phe Arg Val Phe 21025 21030 21035 Ala Lys Asn Glu Lys Gly Leu Ser Asp Pro Val Thr Ile Gly Pro 21040 21045 21050 Ile Thr Val Lys Glu Leu Ile Ile Thr Pro Glu Val Asp Leu Ser 21055 21060 21065 Asp Ile Pro Gly Ala Gln Val Thr Val Arg Ile Gly His Asn Val 21070 21075 21080 His Leu Glu Leu Pro Tyr Lys Gly Lys Pro Lys Pro Ser Ile Ser 21085 21090 21095 Trp Leu Lys Asp Gly Leu Pro Leu Lys Glu Ser Glu Phe Val Arg 21100 21105 21110 Phe Ser Lys Thr Glu Asn Lys Ile Thr Leu Ser Ile Lys Asn Ala 21115 21120 21125 Lys Lys Glu His Gly Gly Lys Tyr Thr Val Ile Leu Asp Asn Ala 21130 21135 21140 Val Cys Arg Ile Ala Val Pro Ile Thr Val Ile Thr Leu Gly Pro 21145 21150 21155 Pro Ser Lys Pro Lys Gly Pro Ile Arg Phe Asp Glu Ile Lys Ala 21160 21165 21170 Asp Ser Val Ile Leu Ser Trp Asp Val Pro Glu Asp Asn Gly Gly 21175 21180 21185 Gly Glu Ile Thr Cys Tyr Ser Ile Glu Lys Arg Glu Thr Ser Gln 21190 21195 21200 Thr Asn Trp Lys Met Val Cys Ser Ser Val Ala Arg Thr Thr Phe 21205 21210 21215 Lys Val Pro Asn Leu Val Lys Asp Ala Glu Tyr Gln Phe Arg Val 21220 21225 21230 Arg Ala Glu Asn Arg Tyr Gly Val Ser Gln Pro Leu Val Ser Ser 21235 21240 21245 Ile Ile Val Ala Lys His Gln Phe Arg Ile Pro Gly Pro Pro Gly 21250 21255 21260 Lys Pro Val Ile Tyr Asn Val Thr Ser Asp Gly Met Ser Leu Thr 21265 21270 21275 Trp Asp Ala Pro Val Tyr Asp Gly Gly Ser Glu Val Thr Gly Phe 21280 21285 21290 His Val Glu Lys Lys Glu Arg Asn Ser Ile Leu Trp Gln Lys Val 21295 21300 21305 Asn Thr Ser Pro Ile Ser Gly Arg Glu Tyr Arg Ala Thr Gly Leu 21310 21315 21320 Val Glu Gly Leu Asp Tyr Gln Phe Arg Val Tyr Ala Glu Asn Ser 21325 21330 21335 Ala Gly Leu Ser Ser Pro Ser Asp Pro Ser Lys Phe Thr Leu Ala 21340 21345 21350 Val Ser Pro Val Asp Pro Pro Gly Thr Pro Asp Tyr Ile Asp Val 21355 21360 21365 Thr Arg Glu Thr Ile Thr Leu Lys Trp Asn Pro Pro Leu Arg Asp 21370 21375 21380 Gly Gly Ser Lys Ile Val Gly Tyr Ser Ile Glu Lys Arg Gln Gly 21385 21390 21395 Asn Glu Arg Trp Val Arg Cys Asn Phe Thr Asp Val Ser Glu Cys 21400 21405 21410 Gln Tyr Thr Val Thr Gly Leu Ser Pro Gly Asp Arg Tyr Glu Phe 21415 21420 21425 Arg Ile Ile Ala Arg Asn Ala Val Gly Thr Ile Ser Pro Pro Ser 21430 21435 21440 Gln Ser Ser Gly Ile Ile Met Thr Arg Asp Glu Asn Val Pro Pro 21445 21450 21455 Ile Val Glu Phe Gly Pro Glu Tyr Phe Asp Gly Leu Ile Ile Lys 21460 21465 21470 Ser Gly Glu Ser Leu Arg Ile Lys Ala Leu Val Gln Gly Arg Pro 21475 21480 21485 Val Pro Arg Val Thr Trp Phe Lys Asp Gly Val Glu Ile Glu Lys 21490 21495 21500 Arg Met Asn Met Glu Ile Thr Asn Val Leu Gly Ser Thr Ser Leu 21505 21510 21515 Phe Val Arg Asp Ala Thr Arg Asp His Arg Gly Val Tyr Thr Val 21520 21525 21530 Glu Ala Lys Asn Ala Ser Gly Ser Ala Lys Ala Glu Ile Lys Val 21535 21540 21545 Lys Val Gln Asp Thr Pro Gly Lys Val Val Gly Pro Ile Arg Phe 21550 21555 21560 Thr Asn Ile Thr Gly Glu Lys Met Thr Leu Trp Trp Asp Ala Pro 21565 21570 21575 Leu Asn Asp Gly Cys Ala Pro Ile Thr His Tyr Ile Ile Glu Lys 21580 21585 21590 Arg Glu Thr Ser Arg Leu Ala Trp Ala Leu Ile Glu Asp Lys Cys 21595 21600 21605 Glu Ala Gln Ser Tyr Thr Ala Ile Lys Leu Ile Asn Gly Asn Glu 21610 21615 21620 Tyr Gln Phe Arg Val Ser Ala Val Asn Lys Phe Gly Val Gly Arg 21625 21630 21635 Pro Leu Asp Ser Asp Pro Val Val Ala Gln Ile Gln Tyr Thr Val 21640 21645 21650 Pro Asp Ala Pro Gly Ile Pro Glu Pro Ser Asn Ile Thr Gly Asn 21655 21660 21665 Ser Ile Thr Leu Thr Trp Ala Arg Pro Glu Ser Asp Gly Gly Ser 21670 21675 21680 Glu Ile Gln Gln Tyr Ile Leu Glu Arg Arg Glu Lys Lys Ser Thr 21685 21690 21695 Arg Trp Val Lys Val Ile Ser Lys Arg Pro Ile Ser Glu Thr Arg 21700 21705 21710 Phe Lys Val Thr Gly Leu Thr Glu Gly Asn Glu Tyr Glu Phe His 21715 21720 21725 Val Met Ala Glu Asn Ala Ala Gly Val Gly Pro Ala Ser Gly Ile 21730 21735 21740 Ser Arg Leu Ile Lys Cys Arg Glu Pro Val Asn Pro Pro Gly Pro 21745 21750 21755 Pro Thr Val Val Lys Val Thr Asp Thr Ser Lys Thr Thr Val Ser 21760 21765 21770 Leu Glu Trp Ser Lys Pro Val Phe Asp Gly Gly Met Glu Ile Ile 21775 21780 21785 Gly Tyr Ile Ile Glu Met Cys Lys Thr Asp Leu Gly Asp Trp His 21790 21795 21800 Lys Val Asn Ala Glu Ala Cys Val Lys Thr Arg Tyr Thr Val Thr 21805 21810 21815 Asp Leu Gln Ala Gly Glu Glu Tyr Lys Phe Arg Val Ser Ala Ile 21820 21825 21830 Asn Gly Ala Gly Lys Gly Asp Ser Cys Glu Val Thr Gly Thr Ile 21835 21840 21845 Lys Ala Val Asp Arg Leu Thr Ala Pro Glu Leu Asp Ile Asp Ala 21850 21855 21860 Asn Phe Lys Gln Thr His Val Val Arg Ala Gly Ala Ser Ile Arg 21865 21870 21875 Leu Phe Ile Ala Tyr Gln Gly Arg Pro Thr Pro Thr Ala Val Trp 21880 21885 21890 Ser Lys Pro Asp Ser Asn Leu Ser Leu Arg Ala Asp Ile His Thr 21895 21900 21905 Thr Asp Ser Phe Ser Thr Leu Thr Val Glu Asn Cys Asn Arg Asn 21910 21915 21920 Asp Ala Gly Lys Tyr Thr Leu Thr Val Glu Asn Asn Ser Gly Ser 21925 21930 21935 Lys Ser Ile Thr Phe Thr Val Lys Val Leu Asp Thr Pro Gly Pro 21940 21945 21950 Pro Gly Pro Ile Thr Phe Lys Asp Val Thr Arg Gly Ser Ala Thr 21955 21960 21965 Leu Met Trp Asp Ala Pro Leu Leu Asp Gly Gly Ala Arg Ile His 21970 21975 21980 His Tyr Val Val Glu Lys Arg Glu Ala Ser Arg Arg Ser Trp Gln 21985 21990 21995 Val Ile Ser Glu Lys Cys Thr Arg Gln Ile Phe Lys Val Asn Asp 22000 22005 22010 Leu Ala Glu Gly Val Pro Tyr Tyr Phe Arg Val Ser Ala Val Asn 22015 22020 22025 Glu Tyr Gly Val Gly Glu Pro Tyr Glu Met Pro Glu Pro Ile Val 22030 22035 22040 Ala Thr Glu Gln Pro Ala Pro Pro Arg Arg Leu Asp Val Val Asp 22045 22050 22055 Thr Ser Lys Ser Ser Ala Val Leu Ala Trp Leu Lys Pro Asp His 22060 22065 22070 Asp Gly Gly Ser Arg Ile Thr Gly Tyr Leu Leu Glu Met Arg Gln 22075 22080 22085 Lys Gly Ser Asp Leu Trp Val Glu Ala Gly His Thr Lys Gln Leu 22090 22095 22100 Thr Phe Thr Val Glu Arg Leu Val Glu Lys Thr Glu Tyr Glu Phe 22105 22110 22115 Arg Val Lys Ala Lys Asn Asp Ala Gly Tyr Ser Glu Pro Arg Glu 22120 22125 22130 Ala Phe Ser Ser Val Ile Ile Lys Glu Pro Gln Ile Glu Pro Thr 22135 22140 22145 Ala Asp Leu Thr Gly Ile Thr Asn Gln Leu Ile Thr Cys Lys Ala 22150 22155 22160 Gly Ser Pro Phe Thr Ile Asp Val Pro Ile Ser Gly Arg Pro Ala 22165 22170 22175 Pro Lys Val Thr Trp Lys Leu Glu Glu Met Arg Leu Lys Glu Thr 22180 22185 22190 Asp Arg Val Ser Ile Thr Thr Thr Lys Asp Arg Thr Thr Leu Thr 22195 22200 22205 Val Lys Asp Ser Met Arg Gly Asp Ser Gly Arg Tyr Phe Leu Thr 22210 22215 22220 Leu Glu Asn Thr Ala Gly Val Lys Thr Phe Ser Val Thr Val Val 22225 22230 22235 Val Ile Gly Arg Pro Gly Pro Val Thr Gly Pro Ile Glu Val Ser 22240 22245 22250 Ser Val Ser Ala Glu Ser Cys Val Leu Ser Trp Gly Glu Pro Lys 22255 22260 22265 Asp Gly Gly Gly Thr Glu Ile Thr Asn Tyr Ile Val Glu Lys Arg 22270 22275 22280 Glu Ser Gly Thr Thr Ala Trp Gln Leu Val Asn Ser Ser Val Lys 22285 22290 22295 Arg Thr Gln Ile Lys Val Thr His Leu Thr Lys Tyr Met Glu Tyr 22300 22305 22310 Ser Phe Arg Val Ser Ser Glu Asn Arg Phe Gly Val Ser Lys Pro 22315 22320 22325 Leu Glu Ser Ala Pro Ile Ile Ala Glu His Pro Phe Val Pro Pro 22330 22335 22340 Ser Ala Pro Thr Arg Pro Glu Val Tyr His Val Ser Ala Asn Ala 22345 22350 22355 Met Ser Ile Arg Trp Glu Glu Pro Tyr His Asp Gly Gly Ser Lys 22360 22365 22370 Ile Ile Gly Tyr Trp Val Glu Lys Lys Glu Arg Asn Thr Ile Leu 22375 22380 22385 Trp Val Lys Glu Asn Lys Val Pro Cys Leu Glu Cys Asn Tyr Lys 22390 22395 22400 Val Thr Gly Leu Val Glu Gly Leu Glu Tyr Gln Phe Arg Thr Tyr 22405 22410 22415 Ala Leu Asn Ala Ala Gly Val Ser Lys Ala Ser Glu Ala Ser Arg 22420 22425 22430 Pro Ile Met Ala Gln Asn Pro Val Asp Ala Pro Gly Arg Pro Glu 22435 22440 22445 Val Thr Asp Val Thr Arg Ser Thr Val Ser Leu Ile Trp Ser Ala 22450 22455 22460 Pro Ala Tyr Asp Gly Gly Ser Lys Val Val Gly Tyr Ile Ile Glu 22465 22470 22475 Arg Lys Pro Val Ser Glu Val Gly Asp Gly Arg Trp Leu Lys Cys 22480 22485 22490 Asn Tyr Thr Ile Val Ser Asp Asn Phe Phe Thr Val Thr Ala Leu 22495 22500 22505 Ser Glu Gly Asp Thr Tyr Glu Phe Arg Val Leu Ala Lys Asn Ala 22510 22515 22520 Ala Gly Val Ile Ser Lys Gly Ser Glu Ser Thr Gly Pro Val Thr 22525 22530 22535 Cys Arg Asp Glu Tyr Ala Pro Pro Lys Ala Glu Leu Asp Ala Arg 22540 22545 22550 Leu His Gly Asp Leu Val Thr Ile Arg Ala Gly Ser Asp Leu Val 22555 22560 22565 Leu Asp Ala Ala Val Gly Gly Lys Pro Glu Pro Lys Ile Ile Trp 22570 22575 22580 Thr Lys Gly Asp Lys Glu Leu Asp Leu Cys Glu Lys Val Ser Leu 22585 22590 22595 Gln Tyr Thr Gly Lys Arg Ala Thr Ala Val Ile Lys Phe Cys Asp 22600 22605 22610 Arg Ser Asp Ser Gly Lys Tyr Thr Leu Thr Val Lys Asn Ala Ser 22615 22620 22625 Gly Thr Lys Ala Val Ser Val Met Val Lys Val Leu Asp Ser Pro 22630 22635 22640 Gly Pro Cys Gly Lys Leu Thr Val Ser Arg Val Thr Gln Glu Lys 22645 22650 22655 Cys Thr Leu Ala Trp Ser Leu Pro Gln Glu Asp Gly Gly Ala Glu 22660 22665 22670 Ile Thr His Tyr Ile Val Glu Arg Arg Glu Thr Ser Arg Leu Asn 22675 22680 22685 Trp Val Ile Val Glu Gly Glu Cys Pro Thr Leu Ser Tyr Val Val 22690 22695 22700 Thr Arg Leu Ile Lys Asn Asn Glu Tyr Ile Phe Arg Val Arg Ala 22705 22710 22715 Val Asn Lys Tyr Gly Pro Gly Val Pro Val Glu Ser Glu Pro Ile 22720 22725 22730 Val Ala Arg Asn Ser Phe Thr Ile Pro Ser Pro Pro Gly Ile Pro 22735 22740 22745 Glu Glu Val Gly Thr Gly Lys Glu His Ile Ile Ile Gln Trp Thr 22750 22755 22760 Lys Pro Glu Ser Asp Gly Gly Asn Glu Ile Ser Asn Tyr Leu Val 22765 22770 22775 Asp Lys Arg Glu Lys Glu Ser Leu Arg Trp Thr Arg Val Asn Lys 22780 22785 22790 Asp Tyr Val Val Tyr Asp Thr Arg Leu Lys Val Thr Ser Leu Met 22795 22800 22805 Glu Gly Cys Asp Tyr Gln Phe Arg Val Thr Ala Val Asn Ala Ala 22810 22815 22820 Gly Asn Ser Glu Pro Ser Glu Arg Ser Asn Phe Ile Ser Cys Arg 22825 22830 22835 Glu Pro Ser Tyr Thr Pro Gly Pro Pro Ser Ala Pro Arg Val Val 22840 22845 22850 Asp Thr Thr Lys His Ser Ile Ser Leu Ala Trp Thr Lys Pro Met 22855 22860 22865 Tyr Asp Gly Gly Thr Asp Ile Val Gly Tyr Val Leu Glu Met Gln 22870 22875 22880 Glu Lys Asp Thr Asp Gln Trp Tyr Arg Val His Thr Asn Ala Thr 22885 22890 22895 Ile Arg Asn Thr Glu Phe Thr Val Pro Asp Leu Lys Met Gly Gln 22900 22905 22910 Lys Tyr Ser Phe Arg Val Ala Ala Val Asn Val Lys Gly Met Ser 22915 22920 22925 Glu Tyr Ser Glu Ser Ile Ala Glu Ile Glu Pro Val Glu Arg Ile 22930 22935 22940 Glu Ile Pro Asp Leu Glu Leu Ala Asp Asp Leu Lys Lys Thr Val 22945 22950 22955 Thr Ile Arg Ala Gly Ala Ser Leu Arg Leu Met Val Ser Val Ser 22960 22965 22970 Gly Arg Pro Pro Pro Val Ile Thr Trp Ser Lys Gln Gly Ile Asp 22975 22980 22985 Leu Ala Ser Arg Ala Ile Ile Asp Thr Thr Glu Ser Tyr Ser Leu 22990 22995 23000 Leu Ile Val Asp Lys Val Asn Arg Tyr Asp Ala Gly Lys Tyr Thr 23005 23010 23015 Ile Glu Ala Glu Asn Gln Ser Gly Lys Lys Ser Ala Thr Val Leu 23020 23025 23030 Val Lys Val Tyr Asp Thr Pro Gly Pro Cys Pro Ser Val Lys Val 23035 23040 23045 Lys Glu Val Ser Arg Asp Ser Val Thr Ile Thr Trp Glu Ile Pro 23050 23055 23060 Thr Ile Asp Gly Gly Ala Pro Ile Asn Asn Tyr Ile Val Glu Lys 23065 23070 23075 Arg Glu Ala Ala Met Arg Ala Phe Lys Thr Val Thr Thr Lys Cys 23080 23085 23090 Ser Lys Thr Leu Tyr Arg Ile Ser Gly Leu Val Glu Gly Thr Met 23095 23100 23105 His Tyr Phe Arg Val Leu Pro Glu Asn Ile Tyr Gly Ile Gly Glu 23110 23115 23120 Pro Cys Glu Thr Ser Asp Ala Val Leu Val Ser Glu Val Pro Leu 23125 23130 23135 Val Pro Ala Lys Leu Glu Val Val Asp Val Thr Lys Ser Thr Val 23140 23145 23150 Thr Leu Ala Trp Glu Lys Pro Leu Tyr Asp Gly Gly Ser Arg Leu 23155 23160 23165 Thr Gly Tyr Val Leu Glu Ala Cys Lys Ala Gly Thr Glu Arg Trp 23170 23175 23180 Met Lys Val Val Thr Leu Lys Pro Thr Val Leu Glu His Thr Val 23185 23190 23195 Thr Ser Leu Asn Glu Gly Glu Gln Tyr Leu Phe Arg Ile Arg Ala 23200 23205 23210 Gln Asn Glu Lys Gly Val Ser Glu Pro Arg Glu Thr Val Thr Ala 23215 23220 23225 Val Thr Val Gln Asp Leu Arg Val Leu Pro Thr Ile Asp Leu Ser 23230 23235 23240 Thr Met Pro Gln Lys Thr Ile His Val Pro Ala Gly Arg Pro Val 23245 23250 23255 Glu Leu Val Ile Pro Ile Ala Gly Arg Pro Pro Pro Ala Ala Ser 23260 23265 23270 Trp Phe Phe Ala Gly Ser Lys Leu Arg Glu Ser Glu Arg Val Thr 23275 23280 23285 Val Glu Thr His Thr Lys Val Ala Lys Leu Thr Ile Arg Glu Thr 23290 23295 23300 Thr Ile Arg Asp Thr Gly Glu Tyr Thr Leu Glu Leu Lys Asn Val 23305 23310 23315 Thr Gly Thr Thr Ser Glu Thr Ile Lys Val Ile Ile Leu Asp Lys 23320 23325 23330 Pro Gly Pro Pro Thr Gly Pro Ile Lys Ile Asp Glu Ile Asp Ala 23335 23340 23345 Thr Ser Ile Thr Ile Ser Trp Glu Pro Pro Glu Leu Asp Gly Gly 23350 23355 23360 Ala Pro Leu Ser Gly Tyr Val Val Glu Gln Arg Asp Ala His Arg 23365 23370 23375 Pro Gly Trp Leu Pro Val Ser Glu Ser Val Thr Arg Ser Thr Phe 23380 23385 23390 Lys Phe Thr Arg Leu Thr Glu Gly Asn Glu Tyr Val Phe Arg Val 23395 23400 23405 Ala Ala Thr Asn Arg Phe Gly Ile Gly Ser Tyr Leu Gln Ser Glu 23410 23415 23420 Val Ile Glu Cys Arg Ser Ser Ile Arg Ile Pro Gly Pro Pro Glu 23425 23430 23435 Thr Leu Gln Ile Phe Asp Val Ser Arg Asp Gly Met Thr Leu Thr 23440 23445 23450 Trp Tyr Pro Pro Glu Asp Asp Gly Gly Ser Gln Val Thr Gly Tyr 23455 23460 23465 Ile Val Glu Arg Lys Glu Val Arg Ala Asp Arg Trp Val Arg Val 23470 23475 23480 Asn Lys Val Pro Val Thr Met Thr Arg Tyr Arg Ser Thr Gly Leu 23485 23490 23495 Thr Glu Gly Leu Glu Tyr Glu His Arg Val Thr Ala Ile Asn Ala 23500 23505 23510 Arg Gly Ser Gly Lys Pro Ser Arg Pro Ser Lys Pro Ile Val Ala 23515 23520 23525 Met Asp Pro Ile Ala Pro Pro Gly Lys Pro Gln Asn Pro Arg Val 23530 23535 23540 Thr Asp Thr Thr Arg Thr Ser Val Ser Leu Ala Trp Ser Val Pro 23545 23550 23555 Glu Asp Glu Gly Gly Ser Lys Val Thr Gly Tyr Leu Ile Glu Met 23560 23565 23570 Gln Lys Val Asp Gln His Glu Trp Thr Lys Cys Asn Thr Thr Pro 23575 23580 23585 Thr Lys Ile Arg Glu Tyr Thr Leu Thr His Leu Pro Gln Gly Ala 23590 23595 23600 Glu Tyr Arg Phe Arg Val Leu Ala Cys Asn Ala Gly Gly Pro Gly 23605 23610 23615 Glu Pro Ala Glu Val Pro Gly Thr Val Lys Val Thr Glu Met Leu 23620 23625 23630 Glu Tyr Pro Asp Tyr Glu Leu Asp Glu Arg Tyr Gln Glu Gly Ile 23635 23640 23645 Phe Val Arg Gln Gly Gly Val Ile Arg Leu Thr Ile Pro Ile Lys 23650 23655 23660 Gly Lys Pro Phe Pro Ile Cys Lys Trp Thr Lys Glu Gly Gln Asp 23665 23670 23675 Ile Ser Lys Arg Ala Met Ile Ala Thr Ser Glu Thr His Thr Glu 23680 23685 23690 Leu Val Ile Lys Glu Ala Asp Arg Gly Asp Ser Gly Thr Tyr Asp 23695 23700 23705 Leu Val Leu Glu Asn Lys Cys Gly Lys Lys Ala Val Tyr Ile Lys 23710 23715 23720 Val Arg Val Ile Gly Ser Pro Asn Ser Pro Glu Gly Pro Leu Glu 23725 23730 23735 Tyr Asp Asp Ile Gln Val Arg Ser Val Arg Val Ser Trp Arg Pro 23740 23745 23750 Pro Ala Asp Asp Gly Gly Ala Asp Ile Leu Gly Tyr Ile Leu Glu 23755 23760 23765 Arg Arg Glu Val Pro Lys Ala Ala Trp Tyr Thr Ile Asp Ser Arg 23770 23775 23780 Val Arg Gly Thr Ser Leu Val Val Lys Gly Leu Lys Glu Asn Val 23785 23790 23795 Glu Tyr His Phe Arg Val Ser Ala Glu Asn Gln Phe Gly Ile Ser 23800 23805 23810 Lys Pro Leu Lys Ser Glu Glu Pro Val Thr Pro Lys Thr Pro Leu 23815 23820 23825 Asn Pro Pro Glu Pro Pro Ser Asn Pro Pro Glu Val Leu Asp Val 23830 23835 23840 Thr Lys Ser Ser Val Ser Leu Ser Trp Ser Arg Pro Lys Asp Asp 23845 23850 23855 Gly Gly Ser Arg Val Thr Gly Tyr Tyr Ile Glu Arg Lys Glu Thr 23860 23865 23870 Ser Thr Asp Lys Trp Val Arg His Asn Lys Thr Gln Ile Thr Thr 23875 23880 23885 Thr Met Tyr Thr Val Thr Gly Leu Val Pro Asp Ala Glu Tyr Gln 23890 23895 23900 Phe Arg Ile Ile Ala Gln Asn Asp Val Gly Leu Ser Glu Thr Ser 23905 23910 23915 Pro Ala Ser Glu Pro Val Val Cys Lys Asp Pro Phe Asp Lys Pro 23920 23925 23930 Ser Gln Pro Gly Glu Leu Glu Ile Leu Ser Ile Ser Lys Asp Ser 23935 23940 23945 Val Thr Leu Gln Trp Glu Lys Pro Glu Cys Asp Gly Gly Lys Glu 23950 23955 23960 Ile Leu Gly Tyr Trp Val Glu Tyr Arg Gln Ser Gly Asp Ser Ala 23965 23970 23975 Trp Lys Lys Ser Asn Lys Glu Arg Ile Lys Asp Lys Gln Phe Thr 23980 23985 23990 Ile Gly Gly Leu Leu Glu Ala Thr Glu Tyr Glu Phe Arg Val Phe 23995 24000 24005 Ala Glu Asn Glu Thr Gly Leu Ser Arg Pro Arg Arg Thr Ala Met 24010 24015 24020 Ser Ile Lys Thr Lys Leu Thr Ser Gly Glu Ala Pro Gly Ile Arg 24025 24030 24035 Lys Glu Met Lys Asp Val Thr Thr Lys Leu Gly Glu Ala Ala Gln 24040 24045 24050 Leu Ser Cys Gln Ile Val Gly Arg Pro Leu Pro Asp Ile Lys Trp 24055 24060 24065 Tyr Arg Phe Gly Lys Glu Leu Ile Gln Ser Arg Lys Tyr Lys Met 24070 24075 24080 Ser Ser Asp Gly Arg Thr His Thr Leu Thr Val Met Thr Glu Glu 24085 24090 24095 Gln Glu Asp Glu Gly Val Tyr Thr Cys Ile Ala Thr Asn Glu Val 24100 24105 24110 Gly Glu Val Glu Thr Ser Ser Lys Leu Leu Leu Gln Ala Thr Pro 24115 24120 24125 Gln Phe His Pro Gly Tyr Pro Leu Lys Glu Lys Tyr Tyr Gly Ala 24130 24135 24140 Val Gly Ser Thr Leu Arg Leu His Val Met Tyr Ile Gly Arg Pro 24145 24150 24155 Val Pro Ala Met Thr Trp Phe His Gly Gln Lys Leu Leu Gln Asn 24160 24165 24170 Ser Glu Asn Ile Thr Ile Glu Asn Thr Glu His Tyr Thr His Leu 24175 24180 24185 Val Met Lys Asn Val Gln Arg Lys Thr His Ala Gly Lys Tyr Lys 24190 24195 24200 Val Gln Leu Ser Asn Val Phe Gly Thr Val Asp Ala Ile Leu Asp 24205 24210 24215 Val Glu Ile Gln Asp Lys Pro Asp Lys Pro Thr Gly Pro Ile Val 24220 24225 24230 Ile Glu Ala Leu Leu Lys Asn Ser Ala Val Ile Ser Trp Lys Pro 24235 24240 24245 Pro Ala Asp Asp Gly Gly Ser Trp Ile Thr Asn Tyr Val Val Glu 24250 24255 24260 Lys Cys Glu Ala Lys Glu Gly Ala Glu Trp Gln Leu Val Ser Ser 24265 24270 24275 Ala Ile Ser Val Thr Thr Cys Arg Ile Val Asn Leu Thr Glu Asn 24280 24285 24290 Ala Gly Tyr Tyr Phe Arg Val Ser Ala Gln Asn Thr Phe Gly Ile 24295 24300 24305 Ser Asp Pro Leu Glu Val Ser Ser Val Val Ile Ile Lys Ser Pro 24310 24315 24320 Phe Glu Lys Pro Gly Ala Pro Gly Lys Pro Thr Ile Thr Ala Val 24325 24330 24335 Thr Lys Asp Ser Cys Val Val Ala Trp Lys Pro Pro Ala Ser Asp 24340 24345 24350 Gly Gly Ala Lys Ile Arg Asn Tyr Tyr Leu Glu Lys Arg Glu Lys 24355 24360 24365 Lys Gln Asn Lys Trp Ile Ser Val Thr Thr Glu Glu Ile Arg Glu 24370 24375 24380 Thr Val Phe Ser Val Lys Asn Leu Ile Glu Gly Leu Glu Tyr Glu 24385 24390 24395 Phe Arg Val Lys Cys Glu Asn Leu Gly Gly Glu Ser Glu Trp Ser 24400 24405 24410 Glu Ile Ser Glu Pro Ile Thr Pro Lys Ser Asp Val Pro Ile Gln 24415 24420 24425 Ala Pro His Phe Lys Glu Glu Leu Arg Asn Leu Asn Val Arg Tyr 24430 24435 24440 Gln Ser Asn Ala Thr Leu Val Cys Lys Val Thr Gly His Pro Lys 24445 24450 24455 Pro Ile Val Lys Trp Tyr Arg Gln Gly Lys Glu Ile Ile Ala Asp 24460 24465 24470 Gly Leu Lys Tyr Arg Ile Gln Glu Phe Lys Gly Gly Tyr His Gln 24475 24480 24485 Leu Ile Ile Ala Ser Val Thr Asp Asp Asp Ala Thr Val Tyr Gln 24490 24495 24500 Val Arg Ala Thr Asn Gln Gly Gly Ser Val Ser Gly Thr Ala Ser 24505 24510 24515 Leu Glu Val Glu Val Pro Ala Lys Ile His Leu Pro Lys Thr Leu 24520 24525 24530 Glu Gly Met Gly Ala Val His Ala Leu Arg Gly Glu Val Val Ser 24535 24540 24545 Ile Lys Ile Pro Phe Ser Gly Lys Pro Asp Pro Val Ile Thr Trp 24550 24555 24560 Gln Lys Gly Gln Asp Leu Ile Asp Asn Asn Gly His Tyr Gln Val 24565 24570 24575 Ile Val Thr Arg Ser Phe Thr Ser Leu Val Phe Pro Asn Gly Val 24580 24585 24590 Glu Arg Lys Asp Ala Gly Phe Tyr Val Val Cys Ala Lys Asn Arg 24595 24600 24605 Phe Gly Ile Asp Gln Lys Thr Val Glu Leu Asp Val Ala Asp Val 24610 24615 24620 Pro Asp Pro Pro Arg Gly Val Lys Val Ser Asp Ala Ser Arg Asp 24625 24630 24635 Ser Val Asn Leu Thr Trp Thr Glu Pro Ala Ser Asp Gly Gly Ser 24640 24645 24650 Lys Ile Thr Asn Tyr Ile Val Glu Lys Cys Ala Thr Thr Ala Glu 24655 24660 24665 Arg Trp Leu Arg Val Gly Gln Ala Arg Glu Thr Arg Tyr Thr Val 24670 24675 24680 Ile Asn Leu Phe Gly Lys Thr Ser Tyr Gln Phe Arg Val Ile Ala 24685 24690 24695 Glu Asn Lys Phe Gly Leu Ser Lys Pro Ser Glu Pro Ser Glu Pro 24700 24705 24710 Thr Ile Thr Lys Glu Asp Lys Thr Arg Ala Met Asn Tyr Asp Glu 24715 24720 24725 Glu Val Asp Glu Thr Arg Glu Val Ser Met Thr Lys Ala Ser His 24730 24735 24740 Ser Ser Thr Lys Glu Leu Tyr Glu Lys Tyr Met Ile Ala Glu Asp 24745 24750 24755 Leu Gly Arg Gly Glu Phe Gly Ile Val His Arg Cys Val Glu Thr 24760 24765 24770 Ser Ser Lys Lys Thr Tyr Met Ala Lys Phe Val Lys Val Lys Gly 24775 24780 24785 Thr Asp Gln Val Leu Val Lys Lys Glu Ile Ser Ile Leu Asn Ile 24790 24795 24800 Ala Arg His Arg Asn Ile Leu His Leu His Glu Ser Phe Glu Ser 24805 24810 24815 Met Glu Glu Leu Val Met Ile Phe Glu Phe Ile Ser Gly Leu Asp 24820 24825 24830 Ile Phe Glu Arg Ile Asn Thr Ser Ala Phe Glu Leu Asn Glu Arg 24835 24840 24845 Glu Ile Val Ser Tyr Val His Gln Val Cys Glu Ala Leu Gln Phe 24850 24855 24860 Leu His Ser His Asn Ile Gly His Phe Asp Ile Arg Pro Glu Asn 24865 24870 24875 Ile Ile Tyr Gln Thr Arg Arg Ser Ser Thr Ile Lys Ile Ile Glu 24880 24885 24890 Phe Gly Gln Ala Arg Gln Leu Lys Pro Gly Asp Asn Phe Arg Leu 24895 24900 24905 Leu Phe Thr Ala Pro Glu Tyr Tyr Ala Pro Glu Val His Gln His 24910 24915 24920 Asp Val Val Ser Thr Ala Thr Asp Met Trp Ser Leu Gly Thr Leu 24925 24930 24935 Val Tyr Val Leu Leu Ser Gly Ile Asn Pro Phe Leu Ala Glu Thr 24940 24945 24950 Asn Gln Gln Ile Ile Glu Asn Ile Met Asn Ala Glu Tyr Thr Phe 24955 24960 24965 Asp Glu Glu Ala Phe Lys Glu Ile Ser Ile Glu Ala Met Asp Phe 24970 24975 24980 Val Asp Arg Leu Leu Val Lys Glu Arg Lys Ser Arg Met Thr Ala 24985 24990 24995 Ser Glu Ala Leu Gln His Pro Trp Leu Lys Gln Lys Ile Glu Arg 25000 25005 25010 Val Ser Thr Lys Val Ile Arg Thr Leu Lys His Arg Arg Tyr Tyr 25015 25020 25025 His Thr Leu Ile Lys Lys Asp Leu Asn Met Val Val Ser Ala Ala 25030 25035 25040 Arg Ile Ser Cys Gly Gly Ala Ile Arg Ser Gln Lys Gly Val Ser 25045 25050 25055 Val Ala Lys Val Lys Val Ala Ser Ile Glu Ile Gly Pro Val Ser 25060 25065 25070 Gly Gln Ile Met His Ala Val Gly Glu Glu Gly Gly His Val Lys 25075 25080 25085 Tyr Val Cys Lys Ile Glu Asn Tyr Asp Gln Ser Thr Gln Val Thr 25090 25095 25100 Trp Tyr Phe Gly Val Arg Gln Leu Glu Asn Ser Glu Lys Tyr Glu 25105 25110 25115 Ile Thr Tyr Glu Asp Gly Val Ala Ile Leu Tyr Val Lys Asp Ile 25120 25125 25130 Thr Lys Leu Asp Asp Gly Thr Tyr Arg Cys Lys Val Val Asn Asp 25135 25140 25145 Tyr Gly Glu Asp Ser Ser Tyr Ala Glu Leu Phe Val Lys Gly Val 25150 25155 25160 Arg Glu Val Tyr Asp Tyr Tyr Cys Arg Arg Thr Met Lys Lys Ile 25165 25170 25175 Lys Arg Arg Thr Asp Thr Met Arg Leu Leu Glu Arg Pro Pro Glu 25180 25185 25190 Phe Thr Leu Pro Leu Tyr Asn Lys Thr Ala Tyr Val Gly Glu Asn 25195 25200 25205 Val Arg Phe Gly Val Thr Ile Thr Val His Pro Glu Pro His Val 25210 25215 25220 Thr Trp Tyr Lys Ser Gly Gln Lys Ile Lys Pro Gly Asp Asn Asp 25225 25230 25235 Lys Lys Tyr Thr Phe Glu Ser Asp Lys Gly Leu Tyr Gln Leu Thr 25240 25245 25250 Ile Asn Ser Val Thr Thr Asp Asp Asp Ala Glu Tyr Thr Val Val 25255 25260 25265 Ala Arg Asn Lys Tyr Gly Glu Asp Ser Cys Lys Ala Lys Leu Thr 25270 25275 25280 Val Thr Leu His Pro Pro Pro Thr Asp Ser Thr Leu Arg Pro Met 25285 25290 25295 Phe Lys Arg Leu Leu Ala Asn Ala Glu Cys Gln Glu Gly Gln Ser 25300 25305 25310 Val Cys Phe Glu Ile Arg Val Ser Gly Ile Pro Pro Pro Thr Leu 25315 25320 25325 Lys Trp Glu Lys Asp Gly Gln Pro Leu Ser Leu Gly Pro Asn Ile 25330 25335 25340 Glu Ile Ile His Glu Gly Leu Asp Tyr Tyr Ala Leu His Ile Arg 25345 25350 25355 Asp Thr Leu Pro Glu Asp Thr Gly Tyr Tyr Arg Val Thr Ala Thr 25360 25365 25370 Asn Thr Ala Gly Ser Thr Ser Cys Gln Ala His Leu Gln Val Glu 25375 25380 25385 Arg Leu Arg Tyr Lys Lys Gln Glu Phe Lys Ser Lys Glu Glu His 25390 25395 25400 Glu Arg His Val Gln Lys Gln Ile Asp Lys Thr Leu Arg Met Ala 25405 25410 25415 Glu Ile Leu Ser Gly Thr Glu Ser Val Pro Leu Thr Gln Val Ala 25420 25425 25430 Lys Glu Ala Leu Arg Glu Ala Ala Val Leu Tyr Lys Pro Ala Val 25435 25440 25445 Ser Thr Lys Thr Val Lys Gly Glu Phe Arg Leu Glu Ile Glu Glu 25450 25455 25460 Lys Lys Glu Glu Arg Lys Leu Arg Met Pro Tyr Asp Val Pro Glu 25465 25470 25475 Pro Arg Lys Tyr Lys Gln Thr Thr Ile Glu Glu Asp Gln Arg Ile 25480 25485 25490 Lys Gln Phe Val Pro Met Ser Asp Met Lys Trp Tyr Lys Lys Ile 25495 25500 25505 Arg Asp Gln Tyr Glu Met Pro Gly Lys Leu Asp Arg Val Val Gln 25510 25515 25520 Lys Arg Pro Lys Arg Ile Arg Leu Ser Arg Trp Glu Gln Phe Tyr 25525 25530 25535 Val Met Pro Leu Pro Arg Ile Thr Asp Gln Tyr Arg Pro Lys Trp 25540 25545 25550 Arg Ile Pro Lys Leu Ser Gln Asp Asp Leu Glu Ile Val Arg Pro 25555 25560 25565 Ala Arg Arg Arg Thr Pro Ser Pro Asp Tyr Asp Phe Tyr Tyr Arg 25570 25575 25580 Pro Arg Arg Arg Ser Leu Gly Asp Ile Ser Asp Glu Glu Leu Leu 25585 25590 25595 Leu Pro Ile Asp Asp Tyr Leu Ala Met Lys Arg Thr Glu Glu Glu 25600 25605 25610 Arg Leu Arg Leu Glu Glu Glu Leu Glu Leu Gly Phe Ser Ala Ser 25615 25620 25625 Pro Pro Ser Arg Ser Pro Pro His Phe Glu Leu Ser Ser Leu Arg 25630 25635 25640 Tyr Ser Ser Pro Gln Ala His Val Lys Val Glu Glu Thr Arg Lys 25645 25650 25655 Asn Phe Arg Tyr Ser Thr Tyr His Ile Pro Thr Lys Ala Glu Ala 25660 25665 25670 Ser Thr Ser Tyr Ala Glu Leu Arg Glu Arg His Ala Gln Ala Ala 25675 25680 25685 Tyr Arg Gln Pro Lys Gln Arg Gln Arg Ile Met Ala Glu Arg Glu 25690 25695 25700 Asp Glu Glu Leu Leu Arg Pro Val Thr Thr Thr Gln His Leu Ser 25705 25710 25715 Glu Tyr Lys Ser Glu Leu Asp Phe Met Ser Lys Glu Glu Lys Ser 25720 25725 25730 Arg Lys Lys Ser Arg Arg Gln Arg Glu Val Thr Glu Ile Thr Glu 25735 25740 25745 Ile Glu Glu Glu Tyr Glu Ile Ser Lys His Ala Gln Arg Glu Ser 25750 25755 25760 Ser Ser Ser Ala Ser Arg Leu Leu Arg Arg Arg Arg Ser Leu Ser 25765 25770 25775 Pro Thr Tyr Ile Glu Leu Met Arg Pro Val Ser Glu Leu Ile Arg 25780 25785 25790 Ser Arg Pro Gln Pro Ala Glu Glu Tyr Glu Asp Asp Thr Glu Arg 25795 25800 25805 Arg Ser Pro Thr Pro Glu Arg Thr Arg Pro Arg Ser Pro Ser Pro 25810 25815 25820 Val Ser Ser Glu Arg Ser Leu Ser Arg Phe Glu Arg Ser Ala Arg 25825 25830 25835 Phe Asp Ile Phe Ser Arg Tyr Glu Ser Met Lys Ala Ala Leu Lys 25840 25845 25850 Thr Gln Lys Thr Ser Glu Arg Lys Tyr Glu Val Leu Ser Gln Gln 25855 25860 25865 Pro Phe Thr Leu Asp His Ala Pro Arg Ile Thr Leu Arg Met Arg 25870 25875 25880 Ser His Arg Val Pro Cys Gly Gln Asn Thr Arg Phe Ile Leu Asn 25885 25890 25895 Val Gln Ser Lys Pro Thr Ala Glu Val Lys Trp Tyr His Asn Gly 25900 25905 25910 Val Glu Leu Gln Glu Ser Ser Lys Ile His Tyr Thr Asn Thr Ser 25915 25920 25925 Gly Val Leu Thr Leu Glu Ile Leu Asp Cys His Thr Asp Asp Ser 25930 25935 25940 Gly Thr Tyr Arg Ala Val Cys Thr Asn Tyr Lys Gly Glu Ala Ser 25945 25950 25955 Asp Tyr Ala Thr Leu Asp Val Thr Gly Gly Asp Tyr Thr Thr Tyr 25960 25965 25970 Ala Ser Gln Arg Arg Asp Glu Glu Val Pro Arg Ser Val Phe Pro 25975 25980 25985 Glu Leu Thr Arg Thr Glu Ala Tyr Ala Val Pro Ser Phe Lys Lys 25990 25995 26000 Thr Ser Glu Met Glu Ala Ser Ser Ser Val Arg Glu Val Lys Ser 26005 26010 26015 Gln Met Thr Glu Thr Arg Glu Ser Leu Ser Ser Tyr Glu His Ser 26020 26025 26030 Ala Ser Ala Glu Met Lys Ser Ala Ala Leu Glu Glu Lys Ser Leu 26035 26040 26045 Glu Glu Lys Ser Thr Thr Arg Lys Ile Lys Thr Thr Leu Ala Ala 26050 26055 26060 Arg Ile Leu Thr Lys Pro Arg Ser Met Thr Val Tyr Glu Gly Glu 26065 26070 26075 Ser Ala Arg Phe Ser Cys Asp Thr Asp Gly Glu Pro Val Pro Thr 26080 26085 26090 Val Thr Trp Leu Arg Lys Gly Gln Val Leu Ser Thr Ser Ala Arg 26095 26100 26105 His Gln Val Thr Thr Thr Lys Tyr Lys Ser Thr Phe Glu Ile Ser 26110 26115 26120 Ser Val Gln Ala Ser Asp Glu Gly Asn Tyr Ser Val Val Val Glu 26125 26130 26135 Asn Ser Glu Gly Lys Gln Glu Ala Glu Phe Thr Leu Thr Ile Gln 26140 26145 26150 Lys Ala Arg Val Thr Glu Lys Ala Val Thr Ser Pro Pro Arg Val 26155 26160 26165 Lys Ser Pro Glu Pro Arg Val Lys Ser Pro Glu Ala Val Lys Ser 26170 26175 26180 Pro Lys Arg Val Lys Ser Pro Glu Pro Ser His Pro Lys Ala Val 26185 26190 26195 Ser Pro Thr Glu Thr Lys Pro Thr Pro Arg Glu Lys Val Gln His 26200 26205 26210 Leu Pro Val Ser Ala Pro Pro Lys Ile Thr Gln Phe Leu Lys Ala 26215 26220 26225 Glu Ala Ser Lys Glu Ile Ala Lys Leu Thr Cys Val Val Glu Ser 26230 26235 26240 Ser Val Leu Arg Ala Lys Glu Val Thr Trp Tyr Lys Asp Gly Lys 26245 26250 26255 Lys Leu Lys Glu Asn Gly His Phe Gln Phe His Tyr Ser Ala Asp 26260 26265 26270 Gly Thr Tyr Glu Leu Lys Ile Asn Asn Leu Thr Glu Ser Asp Gln 26275 26280 26285 Gly Glu Tyr Val Cys Glu Ile Ser Gly Glu Gly Gly Thr Ser Lys 26290 26295 26300 Thr Asn Leu Gln Phe Met Gly Gln Ala Phe Lys Ser Ile His Glu 26305 26310 26315 Lys Val Ser Lys Ile Ser Glu Thr Lys Lys Ser Asp Gln Lys Thr 26320 26325 26330 Thr Glu Ser Thr Val Thr Arg Lys Thr Glu Pro Lys Ala Pro Glu 26335 26340 26345 Pro Ile Ser Ser Lys Pro Val Ile Val Thr Gly Leu Gln Asp Thr 26350 26355 26360 Thr Val Ser Ser Asp Ser Val Ala Lys Phe Ala Val Lys Ala Thr 26365 26370 26375 Gly Glu Pro Arg Pro Thr Ala Ile Trp Thr Lys Asp Gly Lys Ala 26380 26385 26390 Ile Thr Gln Gly Gly Lys Tyr Lys Leu Ser Glu Asp Lys Gly Gly 26395 26400 26405 Phe Phe Leu Glu Ile His Lys Thr Asp Thr Ser Asp Ser Gly Leu 26410 26415 26420 Tyr Thr Cys Thr Val Lys Asn Ser Ala Gly Ser Val Ser Ser Ser 26425 26430 26435 Cys Lys Leu Thr Ile Lys Ala Ile Lys Asp Thr Glu Ala Gln Lys 26440 26445 26450 Val Ser Thr Gln Lys Thr Ser Glu Ile Thr Pro Gln Lys Lys Ala 26455 26460 26465 Val Val Gln Glu Glu Ile Ser Gln Lys Ala Leu Arg Ser Glu Glu 26470 26475 26480 Ile Lys Met Ser Glu Ala Lys Ser Gln Glu Lys Leu Ala Leu Lys 26485 26490 26495 Glu Glu Ala Ser Lys Val Leu Ile Ser Glu Glu Val Lys Lys Ser 26500 26505 26510 Ala Ala Thr Ser Leu Glu Lys Ser Ile Val His Glu Glu Ile Thr 26515 26520 26525 Lys Thr Ser Gln Ala Ser Glu Glu Val Arg Thr His Ala Glu Ile 26530 26535 26540 Lys Ala Phe Ser Thr Gln Met Ser Ile Asn Glu Gly Gln Arg Leu 26545 26550 26555 Val Leu Lys Ala Asn Ile Ala Gly Ala Thr Asp Val Lys Trp Val 26560 26565 26570 Leu Asn Gly Val Glu Leu Thr Asn Ser Glu Glu Tyr Arg Tyr Gly 26575 26580 26585 Val Ser Gly Ser Asp Gln Thr Leu Thr Ile Lys Gln Ala Ser His 26590 26595 26600 Arg Asp Glu Gly Ile Leu Thr Cys Ile Ser Lys Thr Lys Glu Gly 26605 26610 26615 Ile Val Lys Cys Gln Tyr Asp Leu Thr Leu Ser Lys Glu Leu Ser 26620 26625 26630 Asp Ala Pro Ala Phe Ile Ser Gln Pro Arg Ser Gln Asn Ile Asn 26635 26640 26645 Glu Gly Gln Asn Val Leu Phe Thr Cys Glu Ile Ser Gly Glu Pro 26650 26655 26660 Ser Pro Glu Ile Glu Trp Phe Lys Asn Asn Leu Pro Ile Ser Ile 26665 26670 26675 Ser Ser Asn Val Ser Ile Ser Arg Ser Arg Asn Val Tyr Ser Leu 26680 26685 26690 Glu Ile Arg Asn Ala Ser Val Ser Asp Ser Gly Lys Tyr Thr Ile 26695 26700 26705 Lys Ala Lys Asn Phe Arg Gly Gln Cys Ser Ala Thr Ala Ser Leu 26710 26715 26720 Met Val Leu Pro Leu Val Glu Glu Pro Ser Arg Glu Val Val Leu 26725 26730 26735 Arg Thr Ser Gly Asp Thr Ser Leu Gln Gly Ser Phe Ser Ser Gln 26740 26745 26750 Ser Val Gln Met Ser Ala Ser Lys Gln Glu Ala Ser Phe Ser Ser 26755 26760 26765 Phe Ser Ser Ser Ser Ala Ser Ser Met Thr Glu Met Lys Phe Ala 26770 26775 26780 Ser Met Ser Ala Gln Ser Met Ser Ser Met Gln Glu Ser Phe Val 26785 26790 26795 Glu Met Ser Ser Ser Ser Phe Met Gly Ile Ser Asn Met Thr Gln 26800 26805 26810 Leu Glu Ser Ser Thr Ser Lys Met Leu Lys Ala Gly Ile Arg Gly 26815 26820 26825 Ile Pro Pro Lys Ile Glu Ala Leu Pro Ser Asp Ile Ser Ile Asp 26830 26835 26840 Glu Gly Lys Val Leu Thr Val Ala Cys Ala Phe Thr Gly Glu Pro 26845 26850 26855 Thr Pro Glu Val Thr Trp Ser Cys Gly Gly Arg Lys Ile His Ser 26860 26865 26870 Gln Glu Gln Gly Arg Phe His Ile Glu Asn Thr Asp Asp Leu Thr 26875 26880 26885 Thr Leu Ile Ile Met Asp Val Gln Lys Gln Asp Gly Gly Leu Tyr 26890 26895 26900 Thr Leu Ser Leu Gly Asn Glu Phe Gly Ser Asp Ser Ala Thr Val 26905 26910 26915 Asn Ile His Ile Arg Ser Ile 26920 26925 3 21 DNA Danio rerio 3 agggacactc agagaccata g 21 4 40 DNA Danio rerio 4 taatacgact cactataggg gtctgaggat actcgccttc 40 5 27 DNA Danio rerio 5 tttgaaccac ttgaaggtca caccagg 27 6 30 DNA Danio rerio 6 gctaagaatg actatggagt tgccacaagc 30 7 27 DNA Danio rerio 7 tgaaccactt gaaggtcaca ccaggag 27 8 41 DNA Danio rerio 8 taatacgact cactataggg agggacactc agagaccata g 41 9 40 DNA Danio rerio 9 taatacgact cactataggg gtctgaggat actcgccttc 40 10 21 DNA Danio rerio 10 agggacactc agagaccata g 21 11 22 DNA Danio rerio 11 ggcaatgtta ctctctgttg ag 22 

What is claimed is:
 1. A method of determining whether a test subject has, or is at risk of developing, a titin-related disease or condition, said method comprising analyzing a nucleic acid molecule of a sample from the test subject to determine whether the test subject has a mutation in a titin gene, wherein the presence of said mutation is an indication that said test subject has, or is at risk of developing, a titin-related disease.
 2. The method of claim 1, further comprising the step of using nucleic acid molecule primers specific for the titin gene for nucleic acid molecule amplification of the titin gene by the polymerase chain reaction.
 3. The method of claim 1, further comprising the step of sequencing titin nucleic acid molecules from said test subject.
 4. The method of claim 1, wherein said test subject is a mammal.
 5. The method of claim 1, wherein said test subject is human.
 6. The method of claim 1, wherein said disease or condition is heart failure.
 7. The method of claim 1, wherein said mutation is the pickwick mutation.
 8. A method for identifying a compound that can be used to treat or to prevent heart failure, said method comprising contacting an organism comprising a titin mutation and having a phenotype characteristic of heart failure with said compound, and determining the effect of said compound on said phenotype, wherein detection of an improvement in said phenotype indicates the identification of a compound that can be used to treat or to prevent heart failure
 9. The method of claim 8, wherein said organism is a zebrafish.
 10. The method of claim 8, wherein said titin mutation is the pickwick mutation.
 11. A method of treating or preventing heart failure in a patient, said method comprising administering to said patient a compound identified using the method of claim
 8. 12. The method of claim 11, wherein said patient has a mutation in the titin gene.
 13. The method of claim 12, wherein said mutation is the pickwick mutation.
 14. A non-human animal comprising a mutation in a titin gene.
 15. The non-human animal of claim 14, wherein the non-human animal is a zebrafish.
 16. The non-human animal of claim 14, wherein the mutation is in a cardiac-specific exon of said titin gene.
 17. The non-human animal of claim 16, wherein the mutation is in the N2B exon of said titin gene.
 18. The non-human animal of claim 14, wherein the mutation results in the presence of a stop codon in said titin gene.
 19. The non-human animal of claim 14, wherein the mutation is the pickwick mutation. 